LOCUS       XM_023633506            5309 bp    mRNA    linear   MAM 11-DEC-2024
DEFINITION  PREDICTED: Equus caballus argonaute RISC catalytic component 3
            (AGO3), transcript variant X5, mRNA.
ACCESSION   XM_023633506
VERSION     XM_023633506.2
DBLINK      BioProject: PRJNA1194615
KEYWORDS    RefSeq.
SOURCE      Equus caballus (horse)
  ORGANISM  Equus caballus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_091685) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Dec 11, 2024 this sequence version replaced XM_023633506.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_041296265.1-RS_2024_12
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 12/06/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Equus caballus"
                     /mol_type="mRNA"
                     /isolate="H_3958"
                     /db_xref="taxon:9796"
                     /chromosome="2"
                     /sex="female"
                     /tissue_type="Whole blood in anticoagulant"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Ithaca, New York"
                     /collection_date="2018-03-01"
                     /breed="thoroughbred"
     gene            1..5309
                     /gene="AGO3"
                     /note="argonaute RISC catalytic component 3; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 201 long SRA reads, 1 Protein"
                     /db_xref="GeneID:100069387"
     CDS             218..2098
                     /gene="AGO3"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X3"
                     /protein_id="XP_023489274.1"
                     /db_xref="GeneID:100069387"
                     /translation="

MCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    218..559
                     /gene="AGO3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(353..355,398..400,440..442,452..454,506..508,
                     527..529,533..535)
                     /gene="AGO3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    692..1969
                     /gene="AGO3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1103..1105,1115..1117,1151..1162,1169..1171,
                     1193..1195,1202..1204,1214..1216,1226..1228)
                     /gene="AGO3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1307..1309,1313..1315,1523..1525,1937..1939)
                     /gene="AGO3"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5309
                     /gene="AGO3"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

aaagagacctctggcagcagtgtggagaatagatagaggagaaaaaactaatctgagaagccagttaggaggctttttcaatcactagttcaggtttcagtctacatgttacttccttgagaagcagtatttgacaccttactcccccccatccagccattccccaagtctgatttctgccactgccttctacaaagcacaacctgtaattcagttcatgtgtgaagttcttgacattcataatattgatgagcaaccaagacctctgactgattctcatcgggtaaagttcaccaaagagataaaaggtctgaaggttgaagtgactcattgtggaacaatgagacggaaataccgtgtttgtaatgtaacaagaaggcctgccagtcatcaaacctttcctttacagttagaaaatggccaaactgtggagagaacggtagcacagtatttcagagaaaaatatactcttcagctgaagtacccgcaccttccctgtctgcaagtgggacaggagcagaaacatacctatctgccactagaagtctgtaatattgtggcagggcaacgatgtatcaagaagctaacggacaatcagacttctactatgatcaaagcaacagcaagatctgcgccagatagacaagaggaaattagcagactggtaagaagtgcaaattatgaaacagatccatttgttcaggagtttcaatttaaggttcgagatgaaatggcccatgtaactggacgagtacttccagcacctatgctccaatatggaggacggaatcggacagtagcaacgccaagccacggagtctgggacatgcgagggaaacagttccacacaggagttgaaatcaaaatgtgggctatagcttgttttgccacacagaggcagtgcagagaagaaatattgaagggttttacggaccagctgcgtaagatttctaaagatgcagggatgcccattcagggccagccttgcttctgcaaatatgcgcagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactctgggctccagcttattatcgtcatcttgccggggaagacaccagtgtatgcggaggtgaaacgtgtaggagatacacttttgggtatggctacacagtgtgttcaagtcaagaatgtcataaaaacatcccctcagactctctcaaacctgtgcctaaagataaatgttaaactcggagggataaataatattcttgtacctcatcaaagaccttctgtgttccagcaaccagtgatctttttgggagcagatgtcactcatccaccagctggtgatgggaagaagccttctattgctgctgttgtaggtagtatggatgcccacccaagcagatactgtgctacagtaagagttcagagaccccgacaggagatcatccaggacttggcctccatggtccgagaacttctcattcagttttataagtcaactcggttcaaacctactcgtatcatcttttatcgggatggtgtttcagagggacagtttaggcaggtattatattatgaactactagccattcgagaagcctgcatcagtttggagaaagactatcaacctggaataacctatattgtagttcagaagagacatcacactcgattattttgtgctgataggacagaacgggtcggaagaagtggcaatatcccagctggaacaacagttgataccgatattacacatccgtatgagtttgacttttacctctgtagccatgctggaatacagggtaccagccgtccttcacactatcatgttttatgggatgataactgctttactgcagatgaacttcagctgctgacttaccagctctgccacacttatgtgcgctgtacacgatctgtttctatacctgcaccagcgtattacgctcacctggtggcatttagagccagatatcatctcgtggacaaagaacatgacagtgctgaaggaagccacgtttcaggacagagcaatgggcgagatccacaagctcttgccaaggctgtacagattcaccaagataccttacgcacaatgtactttgcttaaaaagtccaagaatgttctctgagaggaagaaccgaaagatgaatcgacatacaacgtatgtttccagtggagtcagttgagtggtgatgcctccaaccatacagaaaccaacactgtttgggggccagggtctgatctttatgttgatgcaaggaagattgtttacttcatcaaggaacacagcaccattatgcaatatgaaaccagccaactgcttttttgtgcggtctcctgtaggaagtatcgcagttgttttgttttcactttcttgtagtctaacccttttaatgcctttacctcaagttgcttggcagcacaactatctttgcaaaaaaagtaaagaaaaagaaaatgatggttttaaaaaaaaaacacacacctacatgaataatcaaagtgattgttcacaattatgtgtgcaaaaaaaataacgtgcattcatgtattcttgtaaaaggtatctatgtatattttaaatatatacatgcatacttcatatgcatatatatctggatctacattgataatagatatatatgtgtctatgtatatattttatagttcattccattggggaactttctttccctcttattcccccatcacgacttttctttctttctcgacctgccttgccttcaccacctacattttttttcctaatgctatcagtgacattcagatgttcctgtatctggttcctttgaatatgaagcatggcaaactagattttaatttttattttgcacaccctactcctgtctcttgtctcatacacaattctgaatcctcaactcttgatagtgctttcttaaaaaacagaattattttatatatatatatttgtttttgtgagagtgtgtgtctgccccctcctgctctgaacttacatttgccatttgtgcaatgtgtacataagcagtagcaacctaaactattctttttctttagtgagtttgttaaagctgctataaatataaaaccttgtcagaagttgtaactctttctctctataagtgatatgtattctaaagccttctgtttctatgggttatacatggaaatcccttaaagcttttataaagggtaaatattttaaatgattggaagtctgtagggttgtggcctctgaaattggatggaggggcgggggttgttgaggtttttaaaattttttgtttgctagtttacttgctgcctctcataccttaatgtgattttcatatatatatatttttatatatatgtgtgtgtatatataaacatatatgtatgtgttttaaagtatagcttccttttcttttatatttattagaatagtgctttaataattatagaaccatattggccacatcttttatagaaatataatcatgtttattttaagaactgacaacttctttttgctatcttttagtgcaataagcagttttaaaggaaagctgtgtctgacatctttaccacagaaagtgggagaaacaggcacctccataattttaaaagggcaaagtaatacagccttaatttcagaaggaaagttttgtaattttcaagtttaaagcatgtcttttaaaaatcataatgaaaatgaaggaagttaaatttcctaaatgagtctgaccttaaatattagaaataaagttgaattttgatttaagcaggacagtgaatagcttaagatttagaagatatttaaattctttgaaaatagatcatgtttattgaagaatcatactggttaggtgcccattttcatttactaaaatgaatcaggaaaattaaaatgatattttaaaaacatttttgctaacatcatcatcgtaactttccttttaaaattagggttatggtgacaaaggaagttaggactttgataattttaaatgagttaaatcactgaaatgtggaaaaaagttttgcaaaacttttgttttactgacttcacgttaagatattaaaaagtactataaagctgtcaagaccgagttcatcagtaacattggattcaggggagctttgcactttgtaactcatgaaaattgcattttaattttttggtttttgcactggttcagagtatagatgttaaagattgtttccagtgggcattgttcttaacctttcagtattccatctgcacatgaaaatgaatggattagctctttagtttctctcttacctataatccactcttattgccaatttactgtattttgagcctaaaatctgagcccatgtcctccaataggtatgaaatcttttgtcctctgccagaaaactaggaaagtactgtattttgtacgtttattatattgaagtgtctgaaatgcaatttacaacactgtttcaaagctctttacatgagataatatttgtacagtatatacattttttctcccaaccctgcccctaaaaaagattcctagtaagcatggttctggttggttggttgttttttaacattgaaatttcactgaatatagtcaggaacaacggcaagagaagaaagtagtatatcctagatggtagaaacttgattgctctgtgcttgctcagtatgagcaaaacatcttcctttaacggtgagagttgcagtttcatgtgggaaactttagggcccagggaagcactgtagataccctttccctatttactgttttacctaattttcagttacttagtttcacatgtgctatagtgaagccaatttggaaggaaatacagagggttttttaaatataaagctttacctttgaagtgaattcttttggggggtttctttcttagactttcaacagtgctagtttaaggttaagtttgaggtttcagcatacttgaaagagtatgtatagtgtatttctgttttgcttactaataaattggtatatcaaacctaattacaagaaatcttgtgcttagaattttaacatagaagtctaagtggcaaatactttttgaaatttgttttaattattactatacttctggtgtcgaattccgatcttttttattttttttaaaaaaaggttgttttgaatttaatggtcatgaaacacaatagtgacctttctgtgaagagttgggagttttaatgtatattctggatggcaaatgtgtctgtgtaaagtgcggatacatgttattatcttagagtatcaagttactggcattgaggaccatacatttataatactgtagctgctatatttat

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