LOCUS       XM_020501407            4053 bp    mRNA    linear   VRT 08-JUL-2025
DEFINITION  PREDICTED: Oncorhynchus kisutch NRDE-2, necessary for RNA
            interference, domain containing (nrde2), transcript variant X2,
            mRNA.
ACCESSION   XM_020501407
VERSION     XM_020501407.2
DBLINK      BioProject: PRJNA378663
KEYWORDS    RefSeq.
SOURCE      Oncorhynchus kisutch (coho salmon)
  ORGANISM  Oncorhynchus kisutch
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii;
            Salmoniformes; Salmonidae; Salmoninae; Oncorhynchus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_034187.2) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Dec 9, 2019 this sequence version replaced XM_020501407.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_002021735.2-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/03/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Oncorhynchus kisutch"
                     /mol_type="mRNA"
                     /isolate="150728-3"
                     /db_xref="taxon:8019"
                     /sex="female"
                     /tissue_type="muscle"
                     /dev_stage="juvenile"
                     /geo_loc_name="Canada: Vancouver"
                     /linkage_group="LG14"
                     /note="double haploid"
     gene            1..4053
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 24 long SRA reads, 3 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 18 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:109904200"
     CDS             132..3713
                     /gene="nrde2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform X1"
                     /protein_id="XP_020356996.1"
                     /db_xref="GeneID:109904200"
                     /translation="

MTSHSCDCLHAEAINMALFPAFAGLSNTKAEIAPKELEWLSNQSFRTDDALSLHQRATEKANETQSAETSRHVIPEREDKNDVQSNKKKKKEKKKKKKKKKKHKKKSGEPSPASSGSDSDTIYPSDLLKKAESTPPSEKFQVEGSFVWLDDLQTPTDQPFCVDRRADRANWEYKSLYRGDIARYKRKGSSSLGLNLRSQAVSWAESGPEKKRVDRRQRPNRYFSPTVRQLLMSDGKPTLPRPPPEDTTTTTLPFIPVPSCLEGEAEGGGSAKGQGSWVNPLGVYDSSTSLWLQGKGKAEHGKAEEQPSTPRDNAAVMLAERVEDFNRRLRETPTDTHTWLQFVLFQDEVAGAPGVFADISENESERRKRSVRATLEKKVAILDRAVENNPSCVELKLERLRLCRELWEPAALLKEWKKLVFLHPNSAPLWRKYLLFMQSHFSTFSVSKVNTVYGKCLSTLAAVQDGSMVSHQMLPGTDEHMLAIFLQQCHFLRQAGHSEKAVCLFQGLLDFTFFKPDTVKDLPTRQQVEFFEPFWDSGEPRVGERGASGWRAWMTQQERGGWLQPSEQDDGDEEDQEELEVKDKTWPKWRIWLDVETSREAIHWLPWRPDKTKGQSVEDCEDPDRQVLFDDIGPSMIRLVHPDLQLCLLLSFLHFLGLPADTSCYSTPSCSLLLDDQALLGDVHHPDSPLTSYALPVPGVSPVGHMTSLQGARRGVGLCKPGEEFVDNMFQMVLPLFSAQERAVLSLHWIQYEKLKVLRCVRGRNKKRVKSQGKRSKRLAKRLLKEPENRGRLSLWREYAHLEWLLGNLDEAHKVFDTALGLGVAGGLADPALCDLCLLYAQLEVEEAWRRDTGGAGTTVTTAMSSPAVYILTKLAEGSSYAPFLGQLAPVAVLKARKAYEQAVVSSLPGQEEGAAGQGQSKSRQVKGLVGCYALFQYLTVGVDAADAVYNLARKRLEQHSSMSAQESASSEVMCDIQTLSSVSECEALAVQQVALLRYHTSTNVYPLSHIRLALTSALAHFASSTSLWQLYVQVENRYHSAGRARRFFHSVTKASASVVPRLFAISAEQQRKQLVDSVQRSDCYGDTVSSLPENGLSNRIRSLFENAIATEHGVHCPLLWRMYMNFLVSEGKVEKGRGIFYKALQDVPWAKGLYMDAVQLFPEHVQEFLDLMTEKELRLRVPMEEVDILLED"

     misc_feature    606..908
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(606..632,639..644,654..659,663..665,669..713,
                     720..728,732..740,762..770,786..791,795..800,807..827,
                     849..860,879..908)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    1104..2102
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

attggatacagtggacctgcaatattctgcccaatgtacgggagcccacgattggctacgttggatctgcaatgcgtcaaacaatggcgcctaatattacctgagtagtttaggaaaacggcgcgttcgagatgacgtcacattcatgcgactgtttacacgcagaggctatcaacatggccctatttccagcttttgcaggattatcaaatacaaaagctgagattgcacctaaagaactagaatggctgagcaatcaaagtttccgcactgatgacgctctctctcttcaccaacgagccactgagaaagccaacgaaacacaatcggcagaaacaagcagacacgttataccagagagggaggataagaatgatgtccaatccaacaagaagaaaaagaaggagaagaagaagaagaagaagaaaaagaagaagcacaagaagaagagtggagaaccctctccagcaagcagtggctcagattcggatactatctaccccagtgacctcctaaaaaaggctgagagcactcctcccagtgagaagtttcaggtggaggggtcttttgtgtggttggatgacctacagacccccaccgatcagcccttctgcgtggacaggagagcagaccgtgccaactgggagtacaaatcactttacagaggagatatcgctaggtacaaaaggaaaggctcttcctctctgggtttgaacctgcggtctcaggccgtgagctgggctgagtctggcccagagaagaagagagtggacaggagacagagacccaaccgctacttctcccccaccgtccgccagctgctgatgtctgatggcaaacccacgctgcccagacctccaccagaggacaccacaaccaccaccttacccttcatccctgtgccctcctgtctggaaggtgaggcagaggggggtggctctgccaaaggccagggctcctgggtcaaccccctgggggtgtatgactcctccacgtccctgtggctccaggggaaggggaaggcggagcatggcaaggcagaggaacaaccttctactcccagggacaatgctgccgtcatgctggctgaacgggtggaggacttcaataggaggctgagggaaacccccacagacacacacacctggcttcagtttgtcctcttccaggacgaggtagctggggcgcctggggtgtttgctgacatctctgagaacgagagcgagcggcggaagaggtcagtgcgggccacgctggagaagaaggtagccattttggatcgagcagtggagaataacccgagctgcgtggaactgaagctggagcgactgcggctctgtagggagctgtgggagccggcagccctgctgaaggagtggaaaaagctggtgttcctgcaccccaacagcgcccccctgtggaggaagtacctgctgttcatgcagagccacttcagtaccttctccgtgtcaaaggtcaacacggtatacgggaagtgtctgagcacgctggcggcggtgcaggatgggagcatggtttctcaccagatgctgccgggcactgatgaacacatgctggccatcttcctccagcagtgtcatttcctcaggcaggctggtcactcagagaaagctgtgtgtctgttccagggcctactggacttcaccttcttcaagccagacactgtcaaggacctacccaccagacaacaggtggagttctttgagccattctgggacagtggggagccccgtgtcggggagagaggggccagtggatggagagcctggatgacccagcaggagagagggggctggctccaacccagtgaacaagatgatggagatgaagaagatcaggaggaattggaggtgaaggataaaacctggcccaagtggaggatctggttggatgtggagacgtcacgcgaggccatccattggctgccatggaggccggacaaaaccaagggccaatcggtggaggactgtgaggacccggaccgacaggtgctgtttgatgacattgggccgtctatgatccggctggtccacccagacctccagctatgtcttcttctgtccttcctgcacttcctgggtctgcctgctgacacttcctgttacagcaccccctcctgtagcctgctgctggatgaccaggctctactgggggatgttcaccaccctgactcaccactgacctcttatgccctccctgttcctggggtcagccccgtgggtcacatgacctccctgcagggtgccaggaggggggtggggctgtgtaagccaggggaggagtttgtagacaacatgttccagatggttctgcctctgttctctgctcaggagagagctgtcctctcactacactggatccagtatgagaagctcaaggtgctgaggtgcgtgcgcggcaggaacaagaagcgtgtgaaatcccaggggaagaggagcaagcgcctggccaagcgcctgctgaaggagccagagaaccggggccgcctcagcctgtggagggagtacgcccacctggagtggctgctagggaatttggacgaggcccacaaggtgttcgacacagccctgggactgggcgtggcaggagggctggcagaccctgccctgtgtgacctctgcctgctctacgcccagctggaggtggaggaggcctggcgaagggatacgggcggggcagggacgactgttacgaccgccatgtcttcaccagcggtctatatcctcaccaagctagcagagggtagttcgtatgcccccttcttgggacagttggctccagtggctgtgctgaaggccaggaaggcctatgagcaggctgtggtgagctccctgcctgggcaggaggagggggccgcggggcaggggcaatccaagtccaggcaggtcaaaggtctggtggggtgttacgccctattccagtacctgactgtgggggttgatgctgctgatgctgtctacaacctggccaggaagaggctggagcaacactccagtatgtcagcccaggagtcagctagcagtgaagttatgtgtgacatacagacactgagctctgtctcagagtgtgaggccctggctgtccagcaggtggcgctgctgcgctaccacaccagcaccaacgtgtacccacttagccatatcagactggcgctgacctcggccctggcccactttgcctccagcacttctctgtggcagttgtatgtgcaggtggagaaccgctaccatagcgctggcagggctcgccgcttcttccacagcgtgaccaaggctagtgccagtgtcgtgccacgcctgttcgccatcagcgcagagcagcaaaggaagcaactagtggactctgttcagaggtctgattgctatggtgacaccgtctccagcctgccagagaacggcctgagcaaccgtatccgctcgctgtttgagaatgccatagcaacagagcacggggtccactgtccgttgctgtggaggatgtacatgaacttcctggtgtcagaggggaaggtggaaaagggaagaggtatcttctacaaggctctacaggacgtcccatgggcaaagggtttatacatggacgctgtgcaactcttccctgagcacgtgcaggagttcctggacctgatgaccgagaaggagctcagactgagggttcccatggaggaggtggacatactgctggaagactgaggctctaaaacccttcccacagtgcactgtggcctggcttcctcagacactccactgtggatacccgaagttgtgcactgaccaatagaacaaccaagtgtcctcatgaagaagaccattttacttttcaaacagacgtgtgaataaatgatgttacacttattagtgttaattcaatattttgatggattgcaaatccatatttttatatttccagctttttatggattcttaatccatattttgattaacataatatcctatttttctgtcaaagatttgtttagattagtttttttgtgtgttttttatggactgaaataaatgttttgtttcctaa

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