LOCUS       NM_001104725            2562 bp    mRNA    linear   VRT 24-JUN-2020
DEFINITION  Oryzias latipes taste 1 receptor member 3 (tas1r3), mRNA.
ACCESSION   NM_001104725
VERSION     NM_001104725.1
KEYWORDS    RefSeq.
SOURCE      Oryzias latipes (Japanese medaka)
  ORGANISM  Oryzias latipes
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Atherinomorphae; Beloniformes;
            Adrianichthyidae; Oryziinae; Oryzias.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Nango E, Akiyama S, Maki-Yonekura S, Ashikawa Y, Kusakabe Y,
            Krayukhina E, Maruno T, Uchiyama S, Nuemket N, Yonekura K, Shimizu
            M, Atsumi N, Yasui N, Hikima T, Yamamoto M, Kobayashi Y and
            Yamashita A.
  TITLE     Taste substance binding elicits conformational change of taste
            receptor T1r heterodimer extracellular domains
  JOURNAL   Sci Rep 6, 25745 (2016)
   PUBMED   27160511
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Ishimaru Y, Okada S, Naito H, Nagai T, Yasuoka A, Matsumoto I and
            Abe K.
  TITLE     Two families of candidate taste receptors in fishes
  JOURNAL   Mech. Dev. 122 (12), 1310-1321 (2005)
   PUBMED   16274966
  REMARK    GeneRIF: Medaka T1R1 and T1R3 show the highest degrees of identity
            to mammalian T1R1 and T1R3, respectively.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB200909.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB200909.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN02929489, SAMN02929490
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Oryzias latipes"
                     /mol_type="mRNA"
                     /db_xref="taxon:8090"
                     /chromosome="5"
                     /map="5"
     gene            1..2562
                     /gene="tas1r3"
                     /note="taste 1 receptor member 3"
                     /db_xref="GeneID:100049303"
     CDS             1..2562
                     /gene="tas1r3"
                     /note="taste receptor, type 1, member 3"
                     /codon_start=1
                     /product="taste receptor type 1 member 3 precursor"
                     /protein_id="NP_001098195.1"
                     /db_xref="GeneID:100049303"
                     /translation="

MALCFLLLAVCWLLRPGCSSPNWFNNISTDLFSMPGDVKLGGLFPIKEQSNEVSNDLTKLNSVSCDSLNKDGLGRALVMKYAVEEINANSQLLPGVKLGYKIYNTCRHSAVIVRPALSFLTEKSNGTLSVECNYTDYETDMVAVIGPQSSEMVTVIGKLLGFFLMPQISFGATSDKFSDSLVYPSFFRTVPSDIRQVDAMVQLIKKFNWNWVAVVGSEEEYGQQGVQQFSKKAEDMGVCVAYQGLIPIYDDPKPAIQTIINNIQTTEVKVVVVFSLVSPAVSFFEEVIKKNLTGVWIASSSWAISDKVYSLPNIERIGTVIGFIDETETLELLYPFTEVLFKKIHEASPTEKPEDPYNPCPECWSLSPANVSLVKEESVQRTAFSVYAAVYTVAHALHKLLECNSAACKWSSSTRLYPWKLLEVLKEFSVNISNTSLKFDQNGNPNIGYSVIQRSWERNTHLYPSVGSYRSANLSINESLFKWYTNNSEKPESTCSKNCQIGQVRRVKGFHSCCFDCIDCLEGTYQANQDDLQCTTCPDRQWSSIRSNSCIAPSFDVLEWGAPVSLYLIVAGVILLICQGSVFVLFLMHRGTPVVLASGGALTFATLLSLMGACLSLLLFFGQPGDVVCHIQLPLISIFQTVALSVILAVSLQLLLVTEFPKSVAPHLRTLRGPGSWVFVLICCLVQAGLCGWFVQDVPSLSEYKNNMTINFLHSFLACPVPSEKLAIMQGLNGSMALVSFMCTFMAVKPLHQYNLARDITFSTLIYCVTWVIFIPLYTGFSTSQSTDHKKRSIAHVSFTLVGISALVLAYYLPKCFLLLRKPNLNTPQQFCTFLEGVPPTQTEEEPQPRQEK"

     sig_peptide     1..57
                     /gene="tas1r3"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    94..1473
                     /gene="tas1r3"
                     /note="ligand-binding domain of the T1R taste receptor;
                     Region: PBP1_taste_receptor; cd06363"
                     /db_xref="CDD:380586"
     misc_feature    order(328..333,337..342,349..354,361..363,394..396,
                     463..465,472..474,484..489,679..681)
                     /gene="tas1r3"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:380586"
     misc_feature    order(442..444,511..519,661..663,901..903)
                     /gene="tas1r3"
                     /note="ligand binding site [chemical binding]; other site"
                     /db_xref="CDD:380586"
     misc_feature    1471..1629
                     /gene="tas1r3"
                     /note="Nine Cysteines Domain of family 3 GPCR; Region:
                     NCD3G; pfam07562"
                     /db_xref="CDD:429539"
     misc_feature    1687..2463
                     /gene="tas1r3"
                     /note="type 1 taste receptor subtype 3, member of the
                     class C of seven-transmembrane G protein-coupled
                     receptors; Region: 7tmC_TAS1R3; cd15290"
                     /db_xref="CDD:320417"
     misc_feature    1687..1764
                     /gene="tas1r3"
                     /note="TM helix 1 [structural motif]; Region: TM helix 1"
                     /db_xref="CDD:320417"
     misc_feature    order(1696..1698,1705..1710,1717..1722,1726..1731,
                     1831..1833,1843..1845,1852..1857,1879..1881,1891..1893)
                     /gene="tas1r3"
                     /note="putative dimer interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:320417"
     misc_feature    1798..1863
                     /gene="tas1r3"
                     /note="TM helix 2 [structural motif]; Region: TM helix 2"
                     /db_xref="CDD:320417"
     misc_feature    order(1858..1860,1894..1899,1906..1911,1918..1920,
                     2161..2163,2176..2178,2185..2190,2197..2199,2299..2301,
                     2308..2313,2320..2322,2332..2334,2374..2379,2386..2388,
                     2395..2397,2407..2409)
                     /gene="tas1r3"
                     /note="putative allosteric modulator binding site
                     [chemical binding]; other site"
                     /db_xref="CDD:320417"
     misc_feature    1888..1962
                     /gene="tas1r3"
                     /note="TM helix 3 [structural motif]; Region: TM helix 3"
                     /db_xref="CDD:320417"
     misc_feature    2026..2088
                     /gene="tas1r3"
                     /note="TM helix 4 [structural motif]; Region: TM helix 4"
                     /db_xref="CDD:320417"
     misc_feature    2164..2244
                     /gene="tas1r3"
                     /note="TM helix 5 [structural motif]; Region: TM helix 5"
                     /db_xref="CDD:320417"
     misc_feature    2266..2337
                     /gene="tas1r3"
                     /note="TM helix 6 [structural motif]; Region: TM helix 6"
                     /db_xref="CDD:320417"
     misc_feature    2368..2445
                     /gene="tas1r3"
                     /note="TM helix 7 [structural motif]; Region: TM helix 7"
                     /db_xref="CDD:320417"
     exon            1..200
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            201..501
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            502..858
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1260
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1467
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1588
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1959
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2562
                     /gene="tas1r3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

atggctttgtgcttccttctgctggctgtgtgctggctgttaagacctggctgcagctcacccaattggttcaacaacatttccacagacctcttcagcatgcccggagacgtaaagctcggagggctttttcccatcaaggagcagtccaatgaggtgtcaaatgacttgaccaaactcaacagtgtgtcttgtgacagtttgaataaagacggcctgggccgtgctctggtgatgaaatatgcagtggaggaaatcaacgccaacagccagcttctgcctggagtcaagttgggttataaaatctacaacacgtgcagacactcagctgttattgtgcgccctgctctctccttcctcacagaaaagtcaaacgggactctttctgtagagtgcaattacactgactatgagacagacatggttgccgtaatcggccctcaatcctcagaaatggtgaccgtcatcggaaaacttctgggattctttctgatgcctcagattagctttggggccaccagtgacaaattcagtgacagtcttgtctacccatcattcttccgcacagtgcccagtgatatacggcaggtggatgctatggtgcagctgataaagaaatttaattggaactgggtggcagtggtggggagtgaagaggagtatggacaacaaggtgttcagcagttctccaaaaaagcagaggacatgggtgtttgtgtggcctaccagggcttgattccaatttacgatgatccaaaaccagcgatccaaaccatcattaacaatatccaaaccactgaggtcaaagtggttgtggtgttttctctcgtgagtccagcggtgtcatttttcgaagaggttatcaagaaaaatttgaccggtgtttggattgccagttcaagctgggccataagtgataaagtttattccctccccaacattgaaaggattgggacagtcattggatttatcgatgagacagagaccctagaactgttgtatcctttcactgaggttctgtttaaaaaaatccacgaggcatctccaacagagaagcctgaggacccctacaacccctgcccagagtgctggagcctgtcgcctgcaaatgtcagcctggtgaaagaggagtccgtgcagagaacagcgtttagcgtgtatgctgccgtttacactgtagcacacgcactgcataaattactggagtgcaattcagctgcttgtaagtggagttcaagcacacggctgtatccctggaagctgttagaagtcttaaaggaattctcagtgaacataagtaacaccagtttaaagtttgatcaaaacggtaacccaaatattggctacagtgtgattcaaaggagttgggagaggaacacccatctttatccatctgttggaagctatcgatctgcaaatctgtctattaatgaatcacttttcaaatggtacaccaacaacagtgagaaacctgagtccacttgttcaaaaaattgccaaataggtcaggtgcgtcgagtgaaagggttccattcctgctgctttgactgcatcgactgtttagagggcacttaccaggctaatcaagacgacctccagtgcaccacgtgtcctgaccgccagtggtcttcgatacgcagcaacagttgcattgcccctagttttgatgttttggagtggggcgcacctgtgtccctgtacttgattgtggcaggggttatcctgctcatctgccaggggtccgtgtttgtcctgttcctgatgcatcgagggacaccggtggtgttggcttcggggggcgctctgacttttgctactctgctcagtctgatgggagcctgtttaagcctcctgctcttctttgggcagcctggagatgttgtgtgtcacatacaacttcctctcatctccatatttcaaactgtggctctctccgtcatcctggctgtttcgctgcagctgcttcttgtgaccgagtttccgaagagcgtcgctcctcacctgcgcacactgagaggcccaggcagctgggtgtttgtgctgatctgctgcctggtgcaggctggcctctgtggctggtttgttcaggatgtaccctctctgtctgaatacaagaacaacatgaccataaacttccttcactcattcctggcatgcccagtgccttcagaaaaactcgctattatgcagggtttaaatggttccatggcacttgtgtccttcatgtgcaccttcatggcagtgaagcctcttcatcaatacaatctcgcgagggacatcaccttctccaccttgatttactgcgtgacttgggtgatttttatcccactctacaccggcttttccacatctcagtccactgatcacaagaagagatcgatcgctcatgtttctttcaccctggtcggcatctcagcactagtgttggcttactacctcccaaagtgttttttgctgttgagaaaacccaacctcaacacgccacagcaattctgcactttcctagaaggagtcccacccacacaaaccgaggaggagccacaaccacgacaggaaaaatga

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