LOCUS       XM_078215734            5942 bp    mRNA    linear   VRT 22-DEC-2025
DEFINITION  PREDICTED: Mustelus asterias argonaute RISC catalytic component 2
            (ago2), transcript variant X2, mRNA.
ACCESSION   XM_078215734
VERSION     XM_078215734.1
DBLINK      BioProject: PRJNA1390264
KEYWORDS    RefSeq.
SOURCE      Mustelus asterias (starry smooth-hound)
  ORGANISM  Mustelus asterias
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
            Elasmobranchii; Galeomorphii; Galeoidea; Carcharhiniformes;
            Triakidae; Mustelus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135807) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_964213995.1-RS_2025_12
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 12/19/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Mustelus asterias"
                     /mol_type="mRNA"
                     /db_xref="taxon:112232"
                     /chromosome="7"
     gene            1..5942
                     /gene="ago2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 38 long SRA reads, 5 Proteins, and 99% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     1 sample with support for all annotated introns"
                     /db_xref="GeneID:144495593"
     CDS             129..2423
                     /gene="ago2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X2"
                     /protein_id="XP_078071860.1"
                     /db_xref="GeneID:144495593"
                     /translation="

MSKLFILHSSEHSQAYNVELEVTLPGEGKDRIFKVVIKWVACVSLQALHDALSGRLPAIPFESIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKNIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTVECTVAQYFKDKHKLNLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMKSASFNADPYVREFGIGVRDDMTDVPGRVLQAPSLLYGGRNKTIATPIQGVWDMRGKQFHTGIEVKVWAIACFAPQRQCREDTLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVVLPGKTPVYAEVKRVGDTLLGMATQCVQVKNVLKTSPQTLSNLCLKINVKLGGVNNILVPQGRPAVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQEIIQDLAFMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFNADELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQVHQDTLRTMYFA"

     misc_feature    <201..341
                     /gene="ago2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    369..521
                     /gene="ago2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    522..884
                     /gene="ago2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(678..680,723..725,765..767,777..779,831..833,
                     852..854,858..860)
                     /gene="ago2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1017..2294
                     /gene="ago2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1428..1430,1440..1442,1476..1487,1494..1496,
                     1518..1520,1527..1529,1539..1541,1551..1553)
                     /gene="ago2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1632..1634,1638..1640,1848..1850,2262..2264)
                     /gene="ago2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ggaaattgttgaacacatggttcaacattttaaaaatcagatcttcggtgatcggaagcctgtgtttgatggaaggaagaacctctacacagcaatgcctcttcctatagggcgagacagaaaatactatgtcgaagcttttcatcctacattcatcagaacattcgcaagcatacaatgtggagctagaggtaactctaccaggggaaggaaaggacagaatctttaaggtggtgataaagtgggtggcatgtgtcagtctccaagccttgcatgatgcactttcgggtcgactcccagctattccttttgaatctattcaagcactagatgtagtgatgagacatctaccctccatgaggtacactccagtaggccgttcattcttcacaccatctgaggggtgctctaatcccttgggaggaggcagggaagtgtggtttggatttcaccagtctgtcagaccttcattgtggaaaatgatgcttaatattgatgtatctgctacagcgttttataaagcccagccggtgattgaattcatgtgtgaagttttggattttaaaaatattgaagaacagcaaaaaccattaactgactcccagagagtaaagttcaccaaggagatcaaaggtctcaaggtagagataactcattgtggacaaatgaaaagaaaatatagagtgtgcaatgttaccagaagacctgcaagccatcaaacattcccacttcagcaagagaatggacagactgttgaatgcacagtagcacaatatttcaaggataagcacaaactaaatcttcgctatcctcatttaccatgtctacaagttggacaggaacaaaaacacacatatcttcctcttgaggtgtgtaacattgtagctgggcaaagatgtataaaaaagctgacagacaatcaaacctcaacaatgattagagccactgccaggtctgcaccagacagacaagatgagatcagcaaactgatgaaaagtgctagttttaatgctgacccatatgttcgggaatttggaataggcgtgagagacgacatgacagacgtaccaggccgtgttcttcaggcaccttccttgttgtacggtggtcggaacaagaccattgctactcctattcagggtgtgtgggatatgcggggtaaacagttccatacaggcattgaagtcaaagtctgggcaattgcatgttttgctccacagaggcagtgcagggaagatacactcaaagcattcacagaccagttaaggaaaatatccagggatgcggggatgccgatacaaggccagccatgtttttgtaaatatgcacaaggtgcagacagtgtggaaccaatgttcaggcacctgaagaacacctatgcaggacttcaactagtggtggttgtgcttcctggaaaaactccagtttacgcggaggttaagcgtgtgggcgacacattacttggcatggccacacaatgtgtgcaagtgaaaaatgtgctgaaaacctctccacaaacactttccaacctctgcctgaagatcaacgtaaagctgggaggtgtcaataatatcttggtgccacagggaaggcctgcagtatttcagcagccagtaatattcttgggtgcagatgtaacacatccaccagctggagatggcaaaaaaccttcaattgctgcagtggtgggtagcatggacgctcatccaagtcgttattgtgccacagtccgtgtacagcagcatcgacaggagatcatccaggaccttgctttcatggtcagagaactcctcatccagttctacaaatctacacgcttcaaaccaacacgcattatattctatagggatggagtttcagaaggacagtttcaacaggttctccatcatgagttgctagctatccgagaggcttgtattaaattagaaaaggactaccagcctggcatcacttttattgtggttcaaaagcgccatcacaccagactcttctgtactgataagaatgaaagggttggaaaaagtggcaatattccagcaggcacaacggtggatacaaaaataacgcatccatctgagtttgacttttacctgtgcagccatgctggcattcagggaacaagcagaccatctcactaccatgttctatgggatgataaccgcttcaatgcagatgaacttcagattctcacatatcaactttgccacacatatgttcgatgtacacggtcagtctccattccagccccagcctattatgctcatttggtggctttccgtgccagataccatttggtggacaaggaacatgacagtgctgaagggagtcacacatctggtcagagcaatggccgggatcaacaggctctggctaaagctgtacaggttcaccaggacacactgcgcactatgtattttgcttgacaggtctcctcagttgggatttctgaacagagctgggctaagaccagaccagctgcatgtcccaggcagtgtagtgacaaatgagcagttaatgtgtttctgttgctgtgtacgttgcaataccctgcaggcctctttcagctatgaggtgaaatatgacctggttgctaatgtgtgaatcaagtcacctgtaatgtttttcttgaagaatgaaaagctggaatattgggaggcctgtggtaatcgtctcagtgtgtatagtcactaatggaagttgaataactgcaatcttattctatattagataaaactagacttaagcctggtgtagatagaaagagaaagtagtatatattttgcggaagcattataatatacattagcaactgcatatttgatgggggtttgcttaggattgtaaagttatggaggaatttagaggatggtatgagtctttaaagacatcccgtctgttggcttcaaatggcagaattttgaaactcaaaattgaacatcaacagcattccttttattatatctggagttttatccagtgaattcagcgaaaactactgtaatacagtactgtgaagcatggccatcttgtgagtcagtaaagggagggaaaaaagtcttttaacactgaaagtaagtctatggagcgctagcatttttttccttttagagcaggcaatatattgtgtaggtttgactttctcagcagttctgagaatgcaatcttatgatttccaacagaagaaatgcagtctctttttaagatgtgtttagtagaaaaatattggtgtgtgtttgaagtaattaatctttggagacaatctataaaacataactagaacagatgggcactactattttatatattgagctcttgatatttttgtaacatgctgcaattgtttagttctcagatcttgatacaatccatcagtgatgcaaatgaaatatttttctgctgtgattgtctagtctgccactgctgtggcttgccgccatgtaggctgtaaggattaccatgacaattacttcgccaatttgagttcaattattacagggcagtcagatgagaactatcgaaattgaaacttttgagaatgaagtaattttaaactacctcgtggttattctcgtgtcagcaaaaatagaatgagcacatgccgctgtcacattggcaaagcagttcaaagatggtgctagatttttgccacgaggcaggcatttttattaatggtagtcatctaattgatgtaaactgtttcctggatttaaatccacccccagattgaagccaaacctttttatggttacatacaatatcttttcaatggcctatgcgcacttattaagtgactcaaagcgttgtataaaatatccagttttatatacctcaacccttcagcaaatttttaaatctcatagaaggtgcaaccagcataaatttcagtgttaacaacttatttatgtggccatgaataaaaaccatattgtacataggtctttaggataaaacctttgcttttgaagttatgtatcaaaacatgatgtagatacaggcaactgtattgtagaagagttttataaactgttttcgactatttctgtatctggctgcatggtactttgtacaggaatggtgacaatgctgcaaatcatattacaaaagtgggcatattttgccaaacaagttcagaattttttttaaaatcttctatttatgaaatatgtacttggaaggactgatgaactgtgcttctaaaaatgcagcgagttacaacatattgtcctcatgcttctcattgaatcctgcatttttttttaacttaactagaagagtttgagaaatggatgtagcgaagggcacactaagtgtatcagtctttggtactgaactagtccgattgaggcttctgagtagttgtaaaaaatctgtacacgtgcaggaatgtctttgcaacattcactaagttacaacactaaggcattcaactctccaacgcgggagacccatgttcaagcctgaatcttgactgccgtacatacttgggtttcctgtgtggtgcagccgttagctattatatgggggttttctgaagaaagaagaggggaagttttctgggaagggaaactttacagaaagattaaacacacctaactggtgtgttggccatggaagagttcttgatgtccatgaaacctccttggtggagcgcagcacagtttcaaatgtgcagagctactaatctcgcaattaaaataagtgattgtttagtatgtaggaacagcatgatgagtactcagggctttaaagactacacttggtttttttttattgtaaaagtttttttctgaattggggcaaatgtgtattatcaaaacttaattgtggaaataaggaattaattcactggaattcaggtagaggggtgagctgatgttagaacctaaccccttttaagcatactaaaccagcataataatctgatgaaacgaaagtttatatcattttacttgtttttaaagtgtacaattgaacttcaaatttgtagggctggacagaagttcttaaccaaaggcttaacctgttacttgagctgctccattaattgttgattgagctgccatgttgaaggaaaagttttattgaaaaaatttcaatacaggattctgttgtattcccaatggtttcaccccttcctgtcctgtttacttttaaaacattgactaatgcacttatttggtgacatcctgcatccaactcagctgattttgtaatattttttaaaaagtaaattggcatgtagatttggggagttttagagattacatttccgtagttatttgtttttagttagtattcctgtattggtcattgactatgttgggcttttttttttaaagaactggacaatccctagatacgtatctctgttccagtttctggggcggatttgattattgaagtgtccttagctattaagcaatctctaaccagcactataaaacgtttacatcttgcttcaaagttctgggtgctactgcgagctggcccctgtattctctgttcctgcgctatacatcgacagtcatagtgacattaaatcagagacaatgaatcaataatagaataaaagtgctgtgatcgttggatggcagcagctctttgtgagaatttattgcagtcactgccatttgagacataactagtgctgcaattggctgattaaatctgccttattataagttactgccattggtggccaacattcaattgggatagttctaataatctgcaaaaattgtagataaggagctagattgtaaaacagtttagtatattaagcatgcagcagaattcatcatgggaattaaatcaccattagtgtttttttattgtaaatgtcatttgtgcataattgagaacatttgtgcttgtaaataattttgtaaacttcaatgtaaacaactctagtaaactaaaaggctaataacgtaaacaaatgg

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