LOCUS       XM_077633161            2156 bp    mRNA    linear   INV 25-NOV-2025
DEFINITION  PREDICTED: Amblyomma americanum protein argonaute-2-like
            (LOC144100127), mRNA.
ACCESSION   XM_077633161
VERSION     XM_077633161.1
DBLINK      BioProject: PRJNA1367164
KEYWORDS    RefSeq.
SOURCE      Amblyomma americanum (Lone Star tick)
  ORGANISM  Amblyomma americanum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae;
            Amblyomma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135504) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_052857255.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/21/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Amblyomma americanum"
                     /mol_type="mRNA"
                     /isolate="KBUSLIRL-KWMA"
                     /isolation_source="laboratory"
                     /db_xref="taxon:6943"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Kerrville, Texas"
                     /collection_date="2021-12"
     gene            1..2156
                     /gene="LOC144100127"
                     /note="protein argonaute-2-like; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 3 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:144100127"
     CDS             109..2055
                     /gene="LOC144100127"
                     /codon_start=1
                     /product="protein argonaute-2-like"
                     /protein_id="XP_077489287.1"
                     /db_xref="GeneID:144100127"
                     /translation="

MLNVDMSATAFYEPLPLVDFMCRFFSDSRRVLTPADLRSLRDNQYVRLDRELKGLRIKVTHLPYPRKYKVVKITREAAKDIYFESEGSQISVADYFQSRYRRLSYPNFPCVQSGSPTHPVYIPLEVCELAEGQHCRKKLDESQTAEMIKRTAKPPAKRFLEIRQSVRDMVSTSEKYLREFGVKINSEPTQVVGRVLDPPTLVFENNTMCKPRDGTWDLRGQRFHKAMSMTQWIVLNVSRFTHKDCLDNFIKMLIRIGQELGMRIAQPLAVITFDTNRQPMRTVLTEQRQQYPQLEMVVAVITKATNYAEIKQVAETELSLRTQCILDNNVVQKCNASLIQNLCQKINAKMGGVNNSLLMQEKPKVFHRPVIVIGADVSHPSPGDKIRPSIAACVGSLDPVPSKFHATIRVQIEDSKAKARVEIIKDLKDMVKELLLVFYRTNGYKPLRIVFYRDGVSEGQFLEVRNHEVSAIRLACAEMCPTETYEPPLTFIVVQKRHHTRFMPAHDRDGVGKCRNVPPGTTVDSVVTHPLDFDFFLCSHFGIQGTSRPAHYYIVWDDSNFSADDLQKLSFYLCHTYSRCARSVSIPAPVYYAHLAAFRAKHHIASKLETSGAVSQSSEGGGDAVLTTAQYVEAVKVLQELQASMYFV"

     misc_feature    148..498
                     /gene="LOC144100127"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(310..312,355..357,382..384,394..396,445..447,
                     466..468,472..474)
                     /gene="LOC144100127"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    631..1920
                     /gene="LOC144100127"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1027..1029,1039..1041,1075..1086,1093..1095,
                     1120..1122,1129..1131,1141..1143,1153..1155)
                     /gene="LOC144100127"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1234..1236,1240..1242,1468..1470,1888..1890)
                     /gene="LOC144100127"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cgctcgttcttcagaccgccgggacccaacgagtacaatgacctcggaggaggccgggaggtgtggtttggctactacacgagtgttcgacccgcccaatggaagcccatgcttaatgtcgacatgtcagcaacagcattctacgagccgcttccactggtagacttcatgtgcaggtttttcagcgacagccggcgtgtgttgacacccgcggacttacggtcattgcgcgacaaccagtacgtgcggctggatagggagctcaaaggactccgcattaaagtgacgcaccttccgtacccgcgcaagtacaaagtggtcaagatcacgagggaagctgcgaaggacatctattttgaatcagaaggtagtcagatctccgtcgccgactacttccaaagccgctacaggcgcttgtcgtacccgaacttcccttgcgtgcagagtggcagcccgacgcatccggtctacattcctctggaggtgtgcgagctggccgaaggccagcactgtcggaagaaactcgatgagagtcagaccgccgagatgatcaagcgcacggccaagcccccagccaagcgcttcctggagattcgtcagtctgtgcgcgacatggtcagcacctcggagaagtacctgcgagagttcggcgtcaagatcaactccgaacccactcaggttgtgggcagagtgctcgacccgccaactctggttttcgagaacaacaccatgtgcaagccacgcgacggtacgtgggatctccgagggcagcgtttccacaaggcgatgtccatgacacagtggattgttctcaacgtgagccgcttcacgcacaaagactgcctggacaacttcatcaagatgctgatccgcatcggccaagaactgggcatgcgcattgcgcagccgcttgcggtcatcacgttcgacacaaaccgccagcccatgcggaccgttctcacggagcagcgccagcagtacccgcagttggagatggttgtggctgtgataacaaaagccacaaactacgctgagatcaagcaggtggcagagactgagctctccctgcgcacacaatgcatcttggacaacaacgttgtccaaaagtgtaacgcatcgctcatccaaaacctgtgccaaaagatcaacgccaagatgggcggagtcaacaatagtctcctgatgcaggagaaacccaaggtattccataggcccgtgatcgtcatcggagcagacgtgtcgcacccatcgcctggagacaagatcaggccatccatcgccgcgtgcgtcggaagcctagaccctgtaccgtccaagtttcacgccaccattcgggtacagattgaagactccaaggctaaggcgcgtgtggaaattatcaaggacctgaaggacatggtcaaggaattgctactggttttctaccgcaccaacggttacaagcccctgcggatcgtcttctacagagacggagtgagcgaggggcagttcttggaagtccgtaaccatgaggtgagcgccatccggctggcgtgtgcggaaatgtgtcccaccgagacctacgagccaccactgacgttcatcgtggtccagaagcggcaccacacgaggttcatgcccgcccacgaccgcgacggcgtgggcaagtgtcgcaacgtcccaccaggcacgaccgtagactcggtggtcacgcacccgctggacttcgacttcttcctctgcagccacttcggcatccagggtaccagccggccggcccactactatatcgtgtgggatgactccaacttcagcgcggacgacctgcagaagctcagcttctacctgtgccacacatactcccggtgtgcaaggagcgtgagcatcccggcccctgtgtactacgcgcacctggccgccttccgcgcaaaacaccacatcgccagcaagctggagacgtcaggcgcggtcagccagtcgtctgagggcggcggggacgccgtgttgaccactgcccaatacgtggaggccgtcaaggtgctgcaggaactgcaggcctccatgtacttcgtgtaatggaggaggttctccgctgcccctgcattccggttacggtgagcctggaggcgtaccactgacgtcataccaaacagttctggagaaagcctcttccaacg

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