LOCUS       XM_077538422            3340 bp    mRNA    linear   VRT 18-NOV-2025
DEFINITION  PREDICTED: Festucalex cinctus NRDE-2, necessary for RNA
            interference, domain containing (nrde2), transcript variant X2,
            mRNA.
ACCESSION   XM_077538422
VERSION     XM_077538422.1
DBLINK      BioProject: PRJNA1336712
KEYWORDS    RefSeq.
SOURCE      Festucalex cinctus (girdled pipefish)
  ORGANISM  Festucalex cinctus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Syngnathiaria; Syngnathiformes; Syngnathoidei;
            Syngnathidae; Festucalex.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135422) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051991245.1-RS_2025_11
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 11/17/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Festucalex cinctus"
                     /mol_type="mRNA"
                     /isolate="MCC-2025b"
                     /isolation_source="wild-caught"
                     /db_xref="taxon:1073243"
                     /chromosome="12"
                     /sex="male"
                     /tissue_type="muscle/skin/bone"
                     /dev_stage="adult"
                     /geo_loc_name="Australia: NSW"
                     /collection_date="2024-01-01"
     gene            1..3340
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 3 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 3 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:144031356"
     CDS             137..3082
                     /gene="nrde2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform X2"
                     /protein_id="XP_077394548.1"
                     /db_xref="GeneID:144031356"
                     /translation="

MCRELLKSQPPLLTIPRVCESSDVTNSNRFLPLEDVEGEKKGETGGSAQTSSVNPLGVYDSTTALWLQGKGKQEQTDEHDKHNLETGQEAVLLTGQTEEFNRKLREQPEDTQLWLKFIRYQDELSATVFGGEDEQQAKELQESRKSSYKAVLEKKLSIAERAVASNPSCVTLQREKLRICQELWEPSALAKEWKKLVFLHPNSGPLWREYLLFTQSYFSNFSVSKVNSAYGKCLSMLSAVRDGSMVSHPAQPGIEEDMLDIFTKQCHFLRQSGHSEKALSLFQAVIDFTFFKPDSVRQLSTKQQVEFFEPFWDSGEARVGEWDARGWKAWMVQQERGGWVQPCAEEEEEEEEEEEEEVKDRSQPRWTVWLDVESSREAAHWLPWRPDKAKGQSEDDCEDPERQVLFDDIGPSLICLSSPELQLRLLLNFLSFLGLPVDTVLSANLGHRGLLLEDLSLLTQGSDSRRALTSHNLPSLGVNSVGHMTTLQGTRKWAGLGKHGEKFVNTMFNMLQPVLSSQQRAVLSLSRMQYEKLKVFRCMHGNKKRLRSQSKISKRIAKSLLKEPENRSSLAMWREFAHLEWMLGNLEEARKVFSTATALGGGKGLANPSLCELCLLWAQLEVEDGASGPGGVLKDGSAPPAVCLLTRLAEGNALSPSSSSQPVSPVAVLKARRSYEQALTAALSAFDEDLCSPQDNQKSHVAVQEEKLRLRGLLGCYALFQYLTMGIKAANAIYSQARERMDKLHSALTSDKHTRADAHHSGSNLLVSRLASECEMVAVQQASLLKYHMSVGVFPLATLRDTLTSALLSWPSSAPLWSVYVQVENRYHSAGRARRFFHAVTRDSSCVVPRLFAIVAEQQRKQLVDAAQRSCDKSTALSFLPENGLSNRIRGLFENAIGSEKGVHCPLLWRMYIHYLVSEGKLDKAKGLFYKALQNIPWVKGLYMDAVQLFPEHLQEFVDLMTEKELRVRLPLEELDILLED"

     misc_feature    431..1396
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
     polyA_site      3340
                     /gene="nrde2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

ccacagcgcagccattctgtgtcgactcgaaatctgacgcggccaactggacgtacaagtctctatacagaggagacgtagcaaggtataggaggaaggggatctcatcactgggtctggatcctcgcagacaagaatgtgtcgtgaattgctcaagtctcagccacctctcctgacaatcccaagagtttgtgaaagcagtgatgtcaccaactccaaccgctttcttcctctggaagacgttgagggggagaaaaaaggggagacaggtggaagtgcacagacgtcatccgtgaatcccctcggcgtgtacgactccaccactgccctctggctgcagggaaagggaaaacaggagcagacagatgagcacgacaagcacaacttggagacaggacaggaagctgtgcttttgactggccagactgaagagtttaacaggaaactgagagagcaacctgaggacacccaactctggctgaaatttattagatatcaagatgaattaagtgcaacggtatttggaggtgaggacgagcagcaggccaaagagttacaggagagtcgcaagtcttcgtacaaggccgtgctggagaagaaactgagcattgcagagcgcgctgtggcttccaaccccagctgtgtcactctgcagcgggagaaactcaggatctgccaagagctttgggaaccatcagctctggctaaagagtggaagaaactggtctttctgcacccaaatagcggccctctgtggagggagtatcttctcttcacccaaagctacttcagcaacttcagtgtgtcaaaagtcaactccgcttacgggaagtgtctcagcatgctcagcgctgtccgggatggcagcatggtgtcccacccagcccagccagggattgaagaggatatgctagatatcttcaccaagcagtgtcatttcctccgtcaatccggtcattcggagaaggcgctctctctctttcaagccgtgatcgactttaccttcttcaagcctgacagtgtacgacaactgtccaccaagcagcaggttgaatttttcgagcccttctgggacagcggggaggcaagagttggggagtgggatgccagaggttggaaagcgtggatggtccaacaggagcgaggaggatgggtgcagccctgcgcagaggaagaagaagaggaggaggaggaagaagaggaggaagtgaaggaccgcagccagccacgatggacggtgtggctggatgtggagtcatctcgtgaagctgcccattggttgccttggagacctgataaagccaagggccaatcagaagacgactgtgaggacccagagagacaagtgctgtttgacgacatcggcccttctctcatttgcctgtcctcgccagagctccagctccgcctcctcctcaatttcctgtcatttttggggctcccagtggacactgtgctgtctgctaacctgggtcatcgtggcttgctattggaggacctttctctgctcactcagggtagtgactcccgccgtgctctgacttcccacaacctaccaagccttggggtgaactctgtgggtcacatgaccacccttcagggaaccaggaaatgggcagggcttggaaagcatggtgagaaatttgtcaacacaatgttcaatatgctccaacccgttctttcatctcaacaacgagctgtgttgtcactcagccggatgcagtatgagaaactcaaggtgtttcgttgcatgcacggcaacaaaaaacgtctccgctctcagagcaaaatcagcaagcggattgccaaatcactgctgaaagaacccgaaaaccgctcatctttggcaatgtggcgggagttcgcccacttggagtggatgctcggtaacctggaagaggctcgcaaagtgttttccacagccacagcattgggtgggggcaaagggctggctaacccttccctctgtgagttgtgtctgctgtgggctcaactggaggtggaggatggcgcgagtgggccaggaggggtgctcaaggatggaagtgcacctccggccgtatgtttgctcaccagacttgctgaggggaacgccttgtcgccttcttcgtcatcacagcccgtctctccggttgccgtcctgaaggccaggaggtcttatgagcaagctctgactgccgctttgtctgcattcgatgaagatctctgcagtcctcaggacaaccaaaaaagtcacgtagccgtccaagaagagaagctgaggttgagaggactgctaggctgttacgctctgttccagtacctcactatgggcatcaaagcggcaaacgccatctacagccaggccagagagcggatggacaagctgcacagcgcactcacgtctgacaagcacacccgcgctgatgctcaccactctggcagcaacttgttggttagcaggttagcctccgagtgtgagatggtggcggtgcagcaggcgtcgctgctcaagtaccacatgagcgttggtgtgtttccactggccacactgagagacaccctcacctcggcccttttaagctggccgagtagtgcccctctctggagcgtttatgtacaggtggagaatcgttaccatagtgcaggccgggcacgtcgtttttttcacgctgtaacaagggacagcagctgtgttgtgccacggctctttgctattgttgctgaacaacaaaggaagcagctggtggatgcagcacagagatcctgtgacaaaagcaccgccttgagcttcctgccagagaatggtctcagcaaccgtatccgtgggctgtttgaaaacgccattggatccgagaagggcgtccactgtcctttactttggaggatgtacattcactacctggtgtcagagggtaagctggataaagccaaggggctcttctacaaagccttacagaatattccatgggtgaagggactgtacatggatgcagtgcagctgtttcccgagcatcttcaagagtttgtcgatctgatgacagagaaagagctgcgagtccggttgccattagaagaactcgacattctgctggaagactgaaacaaactcacatgcaaaactagaattgggcagctcagcagtagaaagacattgtagtgatttctttagatggacgagaaggacaatctcaatctggacttctgagaagtaaactaaaaaaggcaaatagcatttttgctgaggacaaaaacattttaggtatttttttttactagatattttcagtgaatataattttgtttgtaatttgtatttgattgttttaatttattaaagtatgattgtttctcaaggtta

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