LOCUS       XM_077337559            6794 bp    mRNA    linear   VRT 27-SEP-2025
DEFINITION  PREDICTED: Paroedura picta protein argonaute-1 (LOC143837570),
            transcript variant X5, mRNA.
ACCESSION   XM_077337559
VERSION     XM_077337559.1
DBLINK      BioProject: PRJNA1334391
KEYWORDS    RefSeq.
SOURCE      Paroedura picta
  ORGANISM  Paroedura picta
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Gekkota; Gekkonidae; Gekkoninae;
            Paroedura.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135373) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_049243985.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/25/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Paroedura picta"
                     /mol_type="mRNA"
                     /isolate="Pp20150507F"
                     /db_xref="taxon:143630"
                     /chromosome="5"
                     /sex="female"
                     /tissue_type="blood"
                     /collection_date="2019-05-08"
     gene            1..6794
                     /gene="LOC143837570"
                     /note="protein argonaute-1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     Protein, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 5 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:143837570"
     CDS             192..2072
                     /gene="LOC143837570"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X4"
                     /protein_id="XP_077193674.1"
                     /db_xref="GeneID:143837570"
                     /translation="

MCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    192..533
                     /gene="LOC143837570"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(327..329,372..374,414..416,426..428,480..482,
                     501..503,507..509)
                     /gene="LOC143837570"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    666..1943
                     /gene="LOC143837570"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1077..1079,1089..1091,1125..1136,1143..1145,
                     1167..1169,1176..1178,1188..1190,1200..1202)
                     /gene="LOC143837570"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1281..1283,1287..1289,1497..1499,1911..1913)
                     /gene="LOC143837570"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6794
                     /gene="LOC143837570"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

ggacaggatattcaaggtctccatcaagtggatggccatcgtcagctggaggatgctgcatgaggccttggtgagcggacagatccctgtgcctctggagtccgtccaggcccttgacgttgccatgaggcaccttgcctccatgagtatcagcaacagcattctacaaggcccagcccgtcatagagttcatgtgtgaggtgctggatatccggaacattgatgaacagccaaagcccttgaccgactcccagagagtgcgcttcaccaaggagatcaaaggtttaaaagtggaagtaactcattgtggacagatgaagaggaaataccgagtgtgtaatgttactcggcggccggctagtcatcaaacgttccccctgcagttggagagtggccagactgtggagtgcacagtagcacaatacttcaagcagaaatacaacctgcagctcaagtatcctcacctgccgtgtcttcaggttggccaggagcagaagcacacgtacttacctctggaggtgtgtaacatcgtggccggccagcgctgcatcaagaaactgacagacaatcagacctccaccatgataaaagctaccgcgaggtcggccccggataggcaagaggaaattagccggctgatgaagaacgccagttacaacctggacccgtacatacaggagtttggcataaaggtgaaggatgacatgaccgaagtgacagggagagtccttcctgctccaatcttacagtatggaggtcggaaccgggctatcgctacacccaaccagggggtctgggacatgagggggaagcaattttacaatggcattgagattaaagtctgggccatagcctgctttgcccctcagaagcagtgcagggaagaagtcttgaagaactttacagaccagctgcgcaagatctccaaggatgcagggatgccaatccaggggcagccatgcttctgcaaatacgcacaaggcgcagacagtgtggagcccatgttcaggcaccttaagaacacgtattctgggcttcagctcatcatagtcatcctcccggggaagacgcctgtgtatgcggaggtgaagcgcgtgggtgatacactcctgggaatggccacacagtgtgtgcaggtgaagaacgttgtgaagacttcgccacagaccctctcgaacctctgcctgaagatcaacgtcaagcttggtggaatcaacaacatcctggtgccccatcagcgctctgccgtctttcagcagccagtaatattccttggagcggacgtcacccatccgccggcaggagatgggaagaagccctccataacagccgtggtgggcagcatggatgcccacccgagccgatactgcgcaacagtgcgggtgcagaggccccggcaggagatcattgaggacttgtcgtacatggtgagagagctcctcatccagttctacaaatccacacgcttcaagcccacccgcatcatcttctatcgcgatggagtcccagaggggcagcttccacagattcttcattatgaactcctagccatccgcgatgcctgcatcaaactggagaaagattaccagccgggcatcacttacattgtcgttcagaaacggcatcacactcgcctcttctgtgcagacaaaaatgagaggattggcaaaagtgggaacatcccagcaggaaccacggtggacacaaacatcactcaccccttcgagtttgacttctatctttgcagtcatgctggcattcagggtacgagcagaccttcccattactatgtcctttgggacgacaaccggtttacagcagatgagcttcaaatcctcacctaccagctctgccacacttatgtccgctgcactcgctctgtttccatcccagcaccagcatattacgcccgcttggtggcgtttcgggcgcgataccacctcgtagacaaagagcacgatagcggagaaggcagccacatatctggccagagcaacggccgagaccctcaggcccttgccaaggctgtgcaagttcaccaggacactttacgtaccatgtactttgcttgaaagcctaactttttttacctcactccagaaagctttcagatttgtaggagccatacaaaaaaaaaaaaacaaaaaacaaaaaaaggaagcattgggagcaccttgtgagcatccaatgagaaatcactgcagggcgtgcggtgatgttggaggcaaaaagaccagcaacctccggggcatatgcgggagagccacttaaaccggacggacacacctttgtagtattacaagagactctttgtttttcaggctttccaattacctgaccaaacgaaaacaaaaagtatttgaaccaaggaaatgatctttcttcctgtcagaagaacagcaggttttcattgcaggggggctggggtgggggaaagcaatattagacttttgactgtgaaatagctgtttttggtcctccaatgccaaatcaaaagctggtaaggtttttgtgtgtgtgcgtgtgcgcgtgttgttgttttgggtgggaggggggaagggatttttagagccttaaaaacagatgactagatttataaaaactaatattttaggatacaagatgctttaattggttaaggagaagaaaatccagtagtttatagaagagttttaacgcttagccccctcccccttgaagtaaaattaattttctccccttatatataattaacgtaaggcacccccagcacttaactactgctaagctgaaaagaaaaggaaactgtcttccgtctgttgacgtgggtcagccgctgccccgttttgatgcaacggtcacatgcttaactgtgggccgagtgccttactgctatgctgcaaatatattgatgtttggtcacctttcaagggaaaaagtgagaatcgtacccaacaatacgctcgtctcagtggcaattcacatttctatcttgaaggtgtcctgtttttttggggtttttttttaaacattcacaaagtttgcagttccatattggctcacaacctcaccgcgggggttaaaatttctgtgcactttttaacattacacacacacctttagagagctgctgatagatctatttttcttgttgcctattgatatgtgttgtttctagcatatttcttggatggatttttcatctcttttttattttaaaaaaccctgcatttctgacaaatggggcgtgtctgtttgtgcgcaagagaaaaagcgagggaggaaatacttaatgtaccccaatggcataaatagaaattccattatcaaatgttgacctgacttttttctaaaccaatgaattttttttttattattattattgagtattgtttttatacaaatgtggagtctgttgccaaaatccactcaaatccaattcttttttctcaacggttgaggactttggagcgttcacactgaagatggagtgggttttctgttccatatgaattgcactgcaagcgtcaatccagagtccttaagagggctcagtcttagttctgcagaatccttgaggagcgttttccacctccttttaaataggtccagcatatttgcaaataatcatgatggctgccgctgctgagtttttgctattctgtttcacgtggaccagaaggggatgggacctcccttctgcccttgcaattaaagaaagaaactaatgggctcctctgatctcagaacatcaaaatgcacttttttgtctcagtggttcttgatttgtgtccagcaagaaaatctgttctccccacagtgaatctaggcctcgcttctgcagctaccaagtatcctgcaagggcaaggcagaaggttatgccaggtgatagataatgggtagatcgttgccatttgaatacaaaaataggtatggctatagacgggtcgttaagagagcttgtatatctaaacctgactgttcttctagaattgttcatcgccccccccccccaaacctgccacagtgagcctttgaaaaaacgaacaaattaattacatttggtgccattctcctgtaagcacatccccttgaagcacatgctttgcgactcccgggaacaacactgaccacttttgcaatggtcacactgattcagatctctcattctttcctcacttcttcctacactgcaccgcgggcctctttggagaagaatgttaaactcaccaaaatgctatgcaaacaaaactgaacatctggccaaggcccctcagaatggagccctgagttgggcattatctttcacactgtagccagaatccaaagaacttcttaagatgcgtgaacccagtgggatttctggctttggccagatctccatgttgcaaactcttcacgagtttaaaatttaaaaaaatggttaatttgtgcagcattggagattttttttttaaagcacaagaccaacaccctgctgggggcaccaaattatttgtgtggttctttggatttaatagtgtgggggggttgtatcactcggctgctttgtatcctgaacgattaggatttcgtttgcaatgggcacaagtcgagtggcagccaacgtgtcagaatggaaaagccaacattccataataaggatcccatggatggtcaccctttctcctgaaaggggtaggggagtctgggcatgcccctcctggctttctgtgcaagaaatgctttttcagaaagaaatcaaagttaactgtttcatttccttggcatctcttcttgcagacttgcctccctacctacttggactaagggtctcaatttagtccctagtattgcagctgaaattctctgctgctgctttggggcaaaaacaaaggttgttaggggaggacggtatagaagtataataaataaataaattttaaatttaatttgggtttctgaagcatttaaaacaaaactgactagtgcattgcaaaatgggggggggggaagtgatatggtgcttgtggctttgtaaaaaaattgcttaaaaaatacaaccacacatctttcaggacttgagaatcatcatcatcaatggaccttttccccccaaaatgtttgcactccagtgttaagtagctgctttgaatgggaggggttggcatgaagcaaactgctggggggggggtgtaaaaatgacaaaacacttcctgcccctaaccccacctctggtctctcctcccattcttcacctggtgcatgcggagtttttcactaagtgccgtaaatgatgtcattgctgcaccctctatgaaacaaagtttctgggcttggcgttttatgtccccccccctttcttagatgttaacaattaaatggttagctctacctgcatgttctgtgaaatggaagctctcttgtttcgttcatgcttatctttgacaaaattatgttctgtcgtcttaaatagctgaagaaagcacagaacggcaggttttttgggtttgtcccccccccttgcaactgattgttcagttcagggtgtcgccaggctacgtatgtaatcttgagatcctctgggaacctgtttgggacgttttcaccttgcaccttagactgtaatttgttgctgtgcctctgttccttttgtggctggaaaacattacaaagtcccacatattatgggatgggtagcatcattatgtaaatggatgttggcttgcttttaaaaaaaaatttttttttacctgcactgctgctagttccaggttacgtttctgttcaaacaaacaatgagtgggtgagggaagacttgcgaaggaatgaaacatgttcctcaaaagcatttgaggaacagcacaagtactaggccaagatccactcaactgcacaggcttgtacaaggaaattgcaccagctcattaaaatgtggtttcggtacagcagactccctgatgccttgttgcaaattgcttctttaaattcccctcactttcaaaagctgtttctcatgcaagcatggaagctacttccccaaggaaaaatcaggcctgaagttctcttgggcttatggccccacagttgttttggatcttgatgtccagtgtctaagggcataccaaaatgctactgatatgatgcagatcagcagcaaaagtgattacctttttcatttggtaaatgcaagacttttgagttataaaagtagagcagagtaaatgctatacagaacacaaactcgggacacatgcaaatactcatatttatgtgtgttatttacataaaggtttgtgtatggaaccagcttcttaaaattttgagtgttcttctctgccttttgaagagttctgtatagcattaaaacctatagaacttcctttgagtaaaaagtcaccatacctgctgtattttacccttttaggaccaacaaagcaactactgtccatgccaagctgatattcttggaaagcctaaaaactgaaaaccatgcatggtttttaacaaatcattgtttttgccagtacagtgcaaatgtgtcaattctgtttggggagaaatgggagcaacacagcagaacagcaataaaagtgaaatctgtatctcacgtgtgcacgtaactttgcacttcaaaataattgtggaagttttaaactaatgggaactctggtttgcgttcaaaacttttaaaaaactgttacaaggaagggttgaactgtaaatagagaatgttcacagggccctcgattggatggggtttcgtcttgttggtttttttaattttgaatctgaacgttgtatggtccatttattaaacagatttgaacagcca

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