LOCUS       XM_075173154           13574 bp    mRNA    linear   VRT 11-JUL-2025
DEFINITION  PREDICTED: Calonectris borealis argonaute RISC catalytic component
            3 (AGO3), transcript variant X4, mRNA.
ACCESSION   XM_075173154
VERSION     XM_075173154.1
DBLINK      BioProject: PRJNA1289127
KEYWORDS    RefSeq.
SOURCE      Calonectris borealis (Cory's shearwater)
  ORGANISM  Calonectris borealis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Neoaves; Aequornithes;
            Procellariiformes; Procellariidae; Calonectris.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_134336) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_964195595.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/10/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Calonectris borealis"
                     /mol_type="mRNA"
                     /db_xref="taxon:1323832"
                     /chromosome="25"
     gene            1..13574
                     /gene="AGO3"
                     /note="argonaute RISC catalytic component 3; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 54 long SRA reads, 1 Protein, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     7 samples with support for all annotated introns"
                     /db_xref="GeneID:142092752"
     CDS             178..2130
                     /gene="AGO3"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X3"
                     /protein_id="XP_075029255.1"
                     /db_xref="GeneID:142092752"
                     /translation="

MWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYDADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    <181..228
                     /gene="AGO3"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    229..591
                     /gene="AGO3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(385..387,430..432,472..474,484..486,538..540,
                     559..561,565..567)
                     /gene="AGO3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    724..2001
                     /gene="AGO3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1135..1137,1147..1149,1183..1194,1201..1203,
                     1225..1227,1234..1236,1246..1248,1258..1260)
                     /gene="AGO3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1339..1341,1345..1347,1555..1557,1969..1971)
                     /gene="AGO3"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      13574
                     /gene="AGO3"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gtcttcctgcagtctgtctttacctgaagtatccccactgggagaccttcatccctgttccttcacaggtatacccctgtgggccgctcctttttctcagctccagaaggctatgatcaccccttgggtggaggtagggaggtctggtttggattccatcagtctgttcggcctgccatgtggaagatgatgctcaatattgatgtttctgccactgccttctacaaagcacaacctgtaattcagtttatgtgtgaagttcttgacattcataatattgatgaacaaccaagacctctgactgattctcatcgggtaaaattcaccaaagagattaagggtctaaaggtagaagtgactcattgtggaacgatgagaaggaaataccgtgtttgtaacgtaacaagaaggcctgcaagtcatcaaacctttcctttacagttagaaaatggccagactgtggagagaacagtagcacagtatttcagagagaaatacaacctccagctgaaataccctcatcttccttgcctacaagtgggacaagaacagaaacacacctacctacctttagaagtatgtaacattgtagctggccagcggtgtatcaagaagctaacagacaatcagacatcgactatgataaaggccacagcgagatctgctccagataggcaagaggaaataagcagattggtgagaagtgcaaattatgatgcagatccgtttgttcaagagttccaattcaaagtccgggatgagatggcccatgtgacggggcgcgtgctcccagctccaatgttgcagtatggaggacggaatcgaacagtggcaaccccaagccatggagtatgggacatgcgagggaagcagtttcacactggtgttgagatcaaaatgtgggctatagcctgctttgcaacacagagacaatgcagagaagaaatactgaagggttttacagatcagctgcgcaagatctccaaagatgcagggatgccgatccaaggccagccctgtttctgtaaatacgcccagggtgcagacagcgtggagcccatgtttcgacacctcaagaacacctattcggggcttcagctcatcattgtcatcctgccagggaaaacaccagtgtatgcggaggtgaagcgtgtgggagatacactgttgggaatggccacacagtgtgttcaagtcaagaatgtaattaaaacatctccacagaccctatcaaatctgtgcctgaagattaatgttaagctaggaggaatcaacaacatccttgtacctcatcaacgaccttctgtgttccagcagccagtgatctttttgggagccgatgtcactcaccctcctgctggagatggaaagaagccttccattgctgctgttgtaggtagtatggatgctcacccaagccggtactgtgccacggtgagagttcagcgaccccggcaggaaatcatccaagatctagcctccatggtgagagagcttctcatccagttctacaagtcaacacggttcaagcccacgcgtatcatcttctacagggacggggtctctgaaggacagtttcgacaggttttgtattacgaactgctagccattagagaagcctgcatcagtttggagaaagattaccagcctggaataacctatattgtggtgcagaagcgacatcatacacgattgttctgtgctgatagaacagaaagggttggacgaagtggcaatattccagctggaacaactgtagatacagacattactcatccatatgagtttgatttttacctctgtagccacgctggaatacagggtaccagccgtccctcacactatcatgttttgtgggatgataactgttttactgcagatgaacttcagctgctgacttaccagctctgccacacatacgtgcgctgtacacgatcggtttctatacccgcaccagcgtattacgctcacctggtagcattcagagcacgctaccatcttgtggacaaagaacacgacagtgctgaaggaagccatgtttcaggacaaagcaatgggagagatccacaggcccttgcgaaggctgtacaaattcaccaagacaccttacgcacgatgtacttcgcttaagtcaataaaatgggagcatactcactgaaaggaagaactgaaaaattaagccacatacaatgtatgtttccagtggggttggtttatggggtgtgcctccgatcatgctgaagttgacactgtgcaggggcgagaggctaacccttaaattgacacaaggaagattgtttacttcatcgaggaacacagcactattatgcaatatgaaaccagccaacagcttttttggcgcggtctcctgtaggaagcaccgccatcatttttttttttaatttctcgtagtttaaccctcttatgcctttacctcaagttgctcggcagcacaactatctttgcaaaaaaaagtataataaaataaaaaaatgatggtgtaatttaaaaaaaaaagcaaaccacctttatgggaacaatcacagtgattgtttgggggaggaaagtgtgcaaaaaaaaaaaattaagttttttaccccactgcattgaggaatctttctctcaatacttgccatcaagcattcgaatgtgaacatatccaagaacatttgaatgtacagcactagctagaaaaaattgagggagggaagatgttttgcacactaatccaataatttgaacataatttagccaactgctgcctttgtgtctttcttcacagctttggagttctcggctccaatagttcccttttttaaaagcaaagaagctagtggtattatatgcaggcagtagtgatctaaactcttcttgctccctggtgaatttactaaagctgctgttttacctagtccaaaagtttcaattcttttctatctggcaagtcaaatatgttgaagcttcacgtgagatcacatgtcccaagttttatgggattacatatagtaaacaccagaactgaaactttagagctaaggtctttttggaaaaaagaaaaatgctagtttacttgctgcctctcacaccttaatgtgatttcatatgtatgtgtttttgaagtttagcttccttttgtttcttttatatttattagagtaataataatgctttaatttaattattacagaacatatggtcaacatcaacttttattttttagaaatgtaaccatgtttatttttaaaaaactgacaacttgttttgctatcttttagtgcaataagcgttctaaaggaaaactgtgtccattgagctgtactaaagcagtgtttgtaccactaaccgtggaagaatggacacctccatgattttaaaaggacaaagtaaaagccttaattttgaaaggaaagttttatagtcagaaggaatcttgaattttccttttttaaatcaaaaaatagcaaaaatgcttctagaattctgcaagctttacttgtcactgcagtattttgatttaacaaaggaactggccatatttttggctttgaaatgtggagggtatttgaattaaatggaaagggttttttattaagatctaagtttttacatgcttagttaaatctatttgaatcagtaaaatgtctggaattaataaaagtttttaggcttttttcctctttgataatgactttccttttaaaattaagttactggtaacttcatttcactttgcaaataaagagggctgggatagttctaacatgttagatcactgaaatgtgaaacaaattaatgggactctttcattccactttaaaggtaaaacaatatttaagatgtcagagatgagcatttattcaatgaggggactggggagttttgcactttgtacactcgtgaactgcatttttgactgtttttatttgcactggttcatagtatatatactgcagatcgtttgtatgggcattgctgttatcccagcccttctgaactccacctgctctccacatgaaaaccagtgggttggtataagtagcttatctcctgtcattctcatgaaatccgctgtttgccaatatactgtattttgagcctaaaatctgagcccgttttgcttcaacaggcatgaatctcccccattttttctgccagaaaacaaagtactgtattttgtgcatttgtatattgtattgaaatgtatgtagtgctgttcacaacactgtctccaagagctttactaaacagttatgtatatacacattttatctcccaatatcaaggaagataatgtaaaaagactaactttgcaaagtcagaattgcactggaactagtcgtgacggtgggcagttggccagtggcttggggctcctcggagaggcggtgggagggtgatcgagggaggctcggcagaccccgacgcgtgggagagtctctggggtcctgggagccgcctgccttcgtcagctgcacccgcagacccaggaccggcccagccacgctctttgacttcttaacgcacttgcctttcggcgccttcagataagcctgctgcagggaggttggtgtaaccgaacggtttgagtgaggtggtttccgaataagtcgcgcaaatgtttttcttggtctgtgacctgacagttttctctcagaagcactcttgctagtagttctgtttataaaaggttcaggttaggctttttacagaaaattgttttcaaatagagaatttttaaaaaatattttacaaagacttcttgtggatttctggcggttagatgaaaattcctttttaaaaagtcttttaacactcctttcaaagacactgtatgcttctcatcctgcccgcgctcagtgactatgatttgagaagcttttttgcttgctactttcaccagaaaataagttaaagaaacttcattgggaaagtgacttaaattatttgtcgagctagcattttacctggttttcatgtgaagagtatttggaagttttaat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