LOCUS       XM_066673598           11088 bp    mRNA    linear   VRT 22-JUL-2024
DEFINITION  PREDICTED: Hoplias malabaricus argonaute RISC catalytic component 2
            (ago2), mRNA.
ACCESSION   XM_066673598
VERSION     XM_066673598.1
DBLINK      BioProject: PRJNA1136869
KEYWORDS    RefSeq.
SOURCE      Hoplias malabaricus (trahira)
  ORGANISM  Hoplias malabaricus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Characiformes; Characoidei; Erythrinidae; Hoplias.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089805) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_029633855.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/19/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Hoplias malabaricus"
                     /mol_type="mRNA"
                     /isolate="fHopMal1"
                     /db_xref="taxon:27720"
                     /chromosome="6"
                     /sex="male"
                     /tissue_type="blood"
                     /dev_stage="adult"
                     /geo_loc_name="Brazil: Represa do Rio Monjolinho - Sao
                     Carlos, State of Sao Paulo"
                     /lat_lon="21.985889 S 47.880722 W"
                     /collection_date="2021-04-21"
                     /collected_by="Marcelo de Bello Cioffi"
     gene            1..11088
                     /gene="ago2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 43 Proteins"
                     /db_xref="GeneID:136699134"
     CDS             186..2804
                     /gene="ago2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_066529695.1"
                     /db_xref="GeneID:136699134"
                     /translation="

MYPSGAAGVVEMCERLPSVGSAGSAPPSPPSQEYVFKPPQRPDFGTMGRTIKLQANFFEMEIPKLEMYHYDIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVSIKWVSCVSLQALHEALSGRLPSIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLAAMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFHQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQIHQDTLRTMYFA"

     misc_feature    498..722
                     /gene="ago2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    750..902
                     /gene="ago2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    903..1265
                     /gene="ago2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1059..1061,1104..1106,1146..1148,1158..1160,
                     1212..1214,1233..1235,1239..1241)
                     /gene="ago2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1398..2675
                     /gene="ago2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1809..1811,1821..1823,1857..1868,1875..1877,
                     1899..1901,1908..1910,1920..1922,1932..1934)
                     /gene="ago2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2013..2015,2019..2021,2229..2231,2643..2645)
                     /gene="ago2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gacccgcggagatactttactccagcactccagattaacactgctttactgaaaacaaactaaaaacagtaattctgttgtaggtaacttaactttcccgtttgcgtatgcatttctccacatctgaagagcaagagtaatctcggagcgactggggagagcgcttaaaacacaactaaagtaaaatgtatccgagtggagcagctggcgttgtcgagatgtgtgagcgcctcccctctgtggggtcagcaggttctgctcctccatctcctccatcacaagaatatgtattcaaaccaccccagcggccagactttggcaccatgggcaggacaatcaaacttcaggccaacttctttgagatggaaattcccaaattggagatgtaccattatgacattgacatcaaacctgaaaagtgtccacgacgggtcaaccgtgaaattgttgagcacatggtccagcactttaaaacacagatctttggtgatcgaaagcctgtgtatgatgggaggaaaaatttgtacacagcaatgcccttacccataggaagagacaaagtggagctggaagtaactattcctggagaaggaaaagacagaagctttaaagtttccataaaatgggtctcctgtgttagcttgcaagctcttcatgaagcattgtctggacgactgccaagcatcccttttgagaccattcaggctcttgacgttgtcatgagacatttgccctcaatgaggtacacacctgttggtcgctcattcttcaccccttcagagggttgctcaaacccccttggtggtgggagggaagtctggtttggcttccatcagtctgttcgaccatccctttggaaaatgatgctcaatattgatgtgtctgctactgcattctacaaagctcaaccagtgattgaatttatgtgtgaggttttggatttcaaaagcattgaagaacaacagaagcccctaacagattcccagagagtaaagtttaccaaggaaataaagggtctgaaagtggagatcacacactgtggacagatgaagcggaagtacagagtttgtaatgtaaccaggagaccagcaagtcaccaaacgttccccttgcagcaggagaatggacagaccattgaatgcactgtagctcagtacttcaaagacaagtacaaattagttcttcgatatcctcatctcccatgtttacaggttggccaggaacagaaacatacctaccttcctcttgaggtttgtaacatagtggctggacagagatgcataaagaaattgacagacaatcagacctccactatgattcgtgccacagccaggtcagcccctgaccgacaggatgaaatcagcaaactgatgagaagtgccaactttaatactgatccttatgtgcgtgagtttggggtcatggtgagagatgagatgactgaagtgaatggtcgggttctccaagcaccttcaattctctatggaggaaggaataaggcaatagctaccccgatccaaggcgtctgggacatgagaaacaaacagttccatactggcattgaaatcaaagtgtgggcaattgcctgttttgctcctcagagacaatgcacagaactcttgctaaaagcattcactgaccagctgcgaaagatctcacgtgatgcaggcatgcccatccaaggtcagccctgcttctgtaagtatgcccaaggagctgacagcgtagaacccatgttcaaacatctgaagtacacctaccaaggtctgcagcttgttgtggtcatcttgccaggaaagactcctgtctatgcggaagtgaagcgtgttggggacacagttttaggaatggccacgcagtgtgtccaggtgaagaatgtccagaaaaccactcctcagacactgtctaacctttgcctgaagattaatgtcaagctgggcggagtcaacaatattttgcttccccagggcaggcccttggtgtttcagcagccagtcatttttctgggtgcagatgtgactcatcctccagcaggagacggaaagaagccctctattgctgcagtggttggcagcatggacgcccatcccagtcgatactgtgctacagtgcgggttcagcagcacagacaggatatcattcaggacttggctgcaatggtcagagagctcttgatccagttttacaagtccactcgctttaaacctacacgcataatctactacagagatgggatttcagaaggtcaatttcaccaggttcttcaacatgagctactggcaatccgtgaggcttgcatcaagttggagaaggactaccagccaggcatcacttttgttgtggtccagaaaagacaccataccaggctgttctgcatggacaggaatgaaagagttggaaagagtgggaatatccctgctggcaccacagttgacacgaaaattactcatccatcagaatttgacttttacctttgcagccatgctgggattcaggggactagcaggccatctcactatcatgtgctgtgggatgacaaccatttcacctcagacgagctgcaggtgcttacctaccagctttgccacacctatgtccgttgcacccgctctgtatccattcctgcaccagcctactatgcccaccttgtggctttccgtgcccgctaccatctagtagacaaagaacatgacagtgctgaaggcagtcacacatcaggccagagtaatggcagagatcaacaggccctggccaaagctgtgcagattcaccaggacacactgcgtaccatgtactttgcctgaacccagtgaaagttgagtcttatatcccgaaacagctcgtgtatgtttgttaaatgtgccactttaaccttatggtttcctcagaagaaatttcccaatgttttaactatccaaatctccaaaaaggtgattgaattccaaacatttaaacattcagtgtgacctccatgtatttattagagctctaaattttagtttaaataggaagaagttcctactttatgaatttaaagcaagttgtgaatctgttatgtgtttatgttgtaagctaggggttcacctgtttacatataaaagtttccatttcaatttttttctttacactgacagatgcatttttatttctgtattattaatatcatgcaaagtcaagttgttgagattctacagtctaagtacttttgaacaactcatatcattgaggttgcaagaaagaataatacagatcatgttccagtgttgagttgcactctccacagctgtattgtcagcaaaatgcactgactaaaggagcacaaattcaggacacaagtacttattttcttttatgtttcatttatctgtacaaacaataatcaatgttgtattttttggcctgacaacatgttgtcctgaactggttcagtctaccttggttgtttgagtaaaaacttgggtttttaaaggttgtgttcatattggagacaaacttgagtatgtgataagggcacattggaccattcagtgggacagtagaatttttataagcttggcatcctccattatcattctttcaccacgagaagaatgttctgacaatcagctctgtgggcttaaatggggtatggtaactagtataggacaggctcctatagtgacaccctccccatttttgagccaagtttgtaaataaggaacctcttattagttcagtgcccttcctcaatttgataatgtatcgtgtgtgtgattttctctaacagtttaatttcaagtgtgagcatttatactctcacatttagactataaagaggaataggaattacatcatacgctcgtttagatatccactgctgaattgatttgttggttgctgtgagaggcattgcttcagactatgaaagtgtgttgtttcattaatcatttcgtctttggtgtgtaagaattctactatcccctaatgtacaattttgtggttatttcagctctatggaattggttgtgccagagaacatttctcactttgcagtgtagttaggtgtcagggtcaaaactggtgctcttgaaagccttgtatgtactgtgttggatgaaaggccagtgttggagaatattggcactgggctgtacacagccattcttcagcaaaccacattcccatatgctgacggcttatgtgaccatggccttacatatagcgaagtcttgagttaagggaaagtattttaaaaacgtgtgtctaactagccacgtttgttcacggagtcacagtggagataaatctattgtctgaatgttatgcaccttattttttttgcccaaatgtcaatgattaagcaagagcctttactatagaagattctttttgtgtgtgtgtgatttctttgcctcccttgactctggaggtctttttttatgagctttttattgccacgtgatttggccagtaggagagtgattttcgttgcatggctttataggtttcattgcagattgatcatccacttgtaattaaaactaaaccgcaatatattgtaagtatctctgtatactgttagaatgcaacatttgaatgccttctaaagctacccataatttgtaatagtctgtgctgagtacttagctataacgatcacttccccatataatgttccagtttaaaaattaattagcttagggctgggacatttataaactttttttttctaactatgcatttataaccaagagattttagtgatgtttgtgtatatgaaatatgaatttacaacatgcgtattgtagtaattttaagctgggtgcttttcttagtgtgcatagtgtgaaactctgtatcagttgtagcagtattagcctacagaatactatattttattatatcagacaattggatatactttgtaagactgtcagtttctcctgttagccaagaatgattacaaatacgtatactgttgtcctacagtgattgaagcacaaaaagataaagtaggtgctaggaatttcctgacaattttttttttaaggccacaatattattttctttatcatgaaatcaccacaaaagcctcaaccatagaactggttgatgaggttgagtttcttcagtttctaaccttgaacgttattcattactgtcattttccgacataactgtgtttgcaggggcattttagttctcaatctaaatacttgattaatttttactgagtggcgctggtctctaaacttgtgaacgtagatgcactttgaagggcaaaggacatacacctttagttctatatgacttaaagcatgaagttactctcttctctggttgtaattgtgtggaaatttctagacgccctacctcttgtcgccggtttctgatattagggtgacatctttgcattagtcatttcttcgtaattacacaagtgttgttcagacttagtgtctttctctcaaagtttctgaacctgattatagatttaacctaggtgtcaaatgcctctaagacattttaaagttactgaaaggggtattttttttcagaggcctagctctgtgccatttaaattaatttaaactgacttccatactttttacaagtaaagtgttaaagcctcaaagaagaattgcttcatgtttaaactgcaaatcacagagtagattagagcattatgagctctcagtctttattgaaagtgtacagtgtgaatactgtcatgttttgccaagcaatttttgattgcaacacattcattttattctgtttgtcccaagtttgtggctgctcatttaaatgtgcataacatgtagttttccattcagattttttctgtagacctggaaatgggttaaggaatcaaatcaagcgaacagtaacatgataactaatacttaggcctgtttaagaaacagaaacctgtttaagaaacatttactttttattgataccattcactttgttccataaatgggatataggtgatcattactttcagttgttactgagtttttagtattttacattttaaagccagcaccttagattgtcaaaacacttccgcatatgcaattcctcaaaatatgaaaaatgtatgattgacatgttttgcactatatgaagcggtgaccaggctattgttggaaaagattgtattgtgagcaaagttctggttgtcctcctttgtcgctgtaaacgctaaaggatactgcagttgtttttgaacaaatttgtattatattttggtacacacatgcacgcacacaaaatgtatattggtgaaaaacacaagtaataagtcctgaagtcctgataactatgcaaagtggaataaattctgatcagagccattgatctcactttaggttttgtgttgttatccattccattaccttttggctgaccccatcaaagattttattttatttttctaaatgtgtatggacaacaattctccacttgaggccctaattaggaatcaacattttctacattaaaactccattcagtgaaaggaatttagaacaatttgtgtttagtgaattttgaatattttccgatgcattttttaaaaatgtattgatgtttctaaaggcatttaactgaaatgatttctaaactataaaattagaatttccttgagttaaaaaacaaaacaaaaacaaatttcaaagctatttcattatactgaacataatgaactggtatgtgattggttgcaatcctgctttctactatgcagagttagaccctttaagagtctgaatatttaaatgaacatggtgctggttttagactaaactgcaatgatttctggatgcaggagtataccaattaaatgtgtcattcttaaatgaagtttgaaatttggcacattgtatagagatgtaaaacagcttagcttatttcactgcactcagatttttaggcagagcagttattttataccagttactgattcactaatcatgccatacagttataataattcgaaatgtttttgaacaatgaatctagtgaaatttttaaatatttcagagtttaaaaagctctgatcttctgacttgaatgttgttctgtatacatgggtcagatgctggggataactcttatcaagaatcaagttatacacaatgagagtactttgtctttaaattctgaaatttaagatttttctaattcagatactttataaaatcacaccctcaaaaaaggccacatataagcttaccagacaaaaaaatggctgtctgcatcaccttgccttaacagcatcaagcctatctgataaggattataccagcctgttctctgttgaatgctagcctaaacttcacattctctgtcatattttagccagagttcatgcgtgtatttcttcactatgtcttttgctccgatatgaagcactatgttagttgacgagtggacaggaattaaggcgaggtatatataataaccaattattaaagaaaacattatgctgagaaattattattattattttttttgtctggggaacctactttgcactgtaatatattttaacagttgtaagcagaattgcaagccattgtctgctactctgttgtattctgataccttttctatattactaatatcttacaataatgtaaacttaggagacaacaccataattttgtctatacccataagaatataatatatctgaatgtactttattacagacaagtaaaatatgcacatgattgtttgagtttgtgaactcaaatgtctcatgcatgcacgtttatagtaactgcagttcagctatgtttcatgggacaaaggactataggatttaatggtgtactcatacaagcatttgggcaattaaggacacacactggcaaaaaaaatcgttccgttgcttgtgtttcaggtgctgcactgtaagaattgtatataatttagggcaacgttgtagtgtttgtttttgcttttagtctgtttatcattgataaaacaaaattaatcttaaagagcatctagtttcgtcctgccactattttgatttatagcgcaatgcccatgttctcctctcaacccaagagtcttggtttatatcactgtgttgtggtataccagatagccataaacgtggtacatgacaagctccagttcactgtgttcataaattaaaggctttgattagcaaattctcacttgacaaatcatgccatagatcagatgccatccaaacaaagtatcttgtttttgtttcatgatacatggtttatatacatggtttgttttagggttccaagagcacagactgaaacataaggccatttttaaataatactacatggaaatctttttttttttttgttgaattgttgaaaaaagaacatatcattgggcttatataatacagatgtacacaaaatactgaaatgttagtgtttctgtgtgcaagactgaagttatggcttaatgacctggctttttataccaactttgaaaactacctcagaatggtgttgggtgctagcgttatatatccctaatttatacgagcatgacaaagaataataatagtgaccaaacaacacaagccaatactgtgtgttagaggtatttagttatattttacatttttgaaacaaaaatttgtattatggactaaaatgagggatgttttgctgtgtgaaaaaaatattggaccaaaatttgagcagaaataaagcttccctttttaaaatgtagtttaaaaaaacaaaacaaaaaaaaacattcaatggactgtgcactcgcattaggtaaatgctgtagtcctaattatttgaccgtttatttatataatcagacacgcttatcagtgggcatcattgcttctgttagtagttttgtttagagatagcaaggcttgagtgctcatttcctgtttgggtcaatgcaaagcaataaatgtggcatttggtcttgtctgcaaggtgcatgtttgtggatactcatttatgtttacttgtagaactccaccctaatggagttcataggctgacatgttgctcttggttttgtcatgaatttttgtaagctgacagtgtcatctccatgcccatcggtagcattgtgctgtattttagattaaatattgggcaagaagggcccaataaccactgctgtttttgcaatattagcggcaaatttggtgcattcaagacaacattcactttttatgggtttagtttggcagcatagcttgaatataactaatcagtttggcaatatgtatcaagcgatattctcagtgtctgtcagttttcacatctgtaaatatgcttgcattattcttgattcctctgtatatccagtattgcaataaaaacatttattaaaacaatctgctaaaagaaaacaccacagtgagggtaagcaggtcaggaataagctatttaaattttgttattgtaaaccaatgtatctacctttactacattttagaaagggaattaattttatatttaaattctcaaattgtaactgaatgtgttgcaacacacactgatacactggcatatttcagttgttctgagagaatttaatccatgtaaaaggtacaaaaggttgtatggaaattaaataattgttctcgtgtcattgagctaaagacatcccgtttgtggtttttgttttgtatttgtgccttttccgatctgtctcaccccctatatttataacgggtgataaacattttcatttgatactccttgccacatcaaagaatttatcatgcaaccttgagtgaatgctgcatagaacgatcagtatttgtattttgcactttctaagcagtaaaaaataaagccctatgtaaaattgggccgacaaaataaaacaaattataagacgaggtatattctgctataataggaatcctaaagaaccatttgactttgctaaaaagcatagagacatgaagtacctcataagcctgatatatgctgcatcaacatatattttacttttgtgaacctcatattgttcaaaacagttgtccctagagggaggtttgatgcacgtacaaggcagtgcatatgcgtttcaaaaatgatttttgattttgtctgcagcttctgtactatggctgttcttggagtgatggaggcatttgctgtagagtgcacagggaatccaacattatattttgaaaagtgtctactttttgtccattcttactgatattttctgtagtttcagcaagcgtcagtaatgtcccctaataaatgatgacttgatatagtcataaatgactgttgactcaggtattgccatttaagggctgtatctgctactgcttcttttctgtgcactctctcacctgtgtcttatggtagccttggaactgcatttgtggataaaacatcatagtctatctggccttaaaaaatgcactactcaccagaacctgcaagttatgctttgctataagtcactgccaatcatgccatatttttgttctatatctacctcctgtgaatgtaggagtgtcctggaaatcaacaagaggtatcttattagagtatatgctaacagctgccctgagcatgtaatcagcaccagaatctccaggacactccagctgaggctgtatgagtttttttccccatgttcatgtaagccatggaaaagtaaagtgaccaaagtttttaccgaagcgctcctgcactgcctagtaggctcaagcctgcccctttcttcagccattttattttattttattttattttattttttgtttcctacttaaccacatgccaaattgaatttgctgtcattcctgttgtgataaggtcttttgtttctcattcttgtgtttgggctcactgtaaagctctgtgtcatcattatttgttgtcaatgttaagagtgagctttgagagtgttttttttttttttaattatgattgcagactcaaaacatgctgtaaaccctgctagaacataatgtgaatacgtaccatttaatatagttgactttcgcttgactgtatattgcattgattaataaacttaagtttgtccggcttgtattatgtcctgtatatattcattttctatagatttttttta

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