LOCUS       XM_066574986            4673 bp    mRNA    linear   VRT 22-JUL-2024
DEFINITION  PREDICTED: Eleutherodactylus coqui argonaute RISC catalytic
            component 3 (AGO3), mRNA.
ACCESSION   XM_066574986
VERSION     XM_066574986.1
DBLINK      BioProject: PRJNA1136876
KEYWORDS    RefSeq.
SOURCE      Eleutherodactylus coqui (Puerto Rican coqui)
  ORGANISM  Eleutherodactylus coqui
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Eleutherodactylidae; Eleutherodactylinae; Eleutherodactylus;
            Eleutherodactylus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089837) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_035609145.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/19/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Eleutherodactylus coqui"
                     /mol_type="mRNA"
                     /strain="aEleCoq1"
                     /db_xref="taxon:57060"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Hawaii"
                     /lat_lon="19.580062 N 154.923937 W"
                     /collection_date="2018-07-06"
                     /collected_by="Mara Laslo"
     gene            1..4673
                     /gene="AGO3"
                     /note="argonaute RISC catalytic component 3; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 43 Proteins"
                     /db_xref="GeneID:136574039"
     CDS             740..3322
                     /gene="AGO3"
                     /codon_start=1
                     /product="protein argonaute-3"
                     /protein_id="XP_066431083.1"
                     /db_xref="GeneID:136574039"
                     /translation="

MEIGTAGPVGAQPFFVVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVASTGVDLDVTLPGEGGKDRPFKVSIKFVSRISWHLLHEVLTGRSLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYDADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIHDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    956..1240
                     /gene="AGO3"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1268..1420
                     /gene="AGO3"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1421..1783
                     /gene="AGO3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1577..1579,1622..1624,1664..1666,1676..1678,
                     1730..1732,1751..1753,1757..1759)
                     /gene="AGO3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1916..3193
                     /gene="AGO3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2327..2329,2339..2341,2375..2386,2393..2395,
                     2417..2419,2426..2428,2438..2440,2450..2452)
                     /gene="AGO3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2531..2533,2537..2539,2747..2749,3161..3163)
                     /gene="AGO3"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

tggaggaccaggcgctgccggcggccgctgctccatcatcgagtgagccgagaagaaggacggctgacgtggaagttgaactggttcttagccaatcccttgtgcgccgctctccgtctttacaagtggcccaccaacagccagtgacgaccaatcacagccggctgtcctcactccgctcaggtggtggtgggggatgccagccaatcacagcccgcctctcatcccgactcgccgctgctagtttggagtagaggacaggtaacgcgagtagccaataggaagagcgagcacgagcgaagagcccggggcgagagctggggacagccaatcagcgagcagcacttcactcacagagcagctgtgacagagatgaggaccaagaggtgactcagccatctctaaggcaacggccagtcagccgtagacctgcgtgtccgcacacctcccggccacctgaagctacaggtgtgacaacaagttacactggtgacaactgtccactgggctgtggtgactagccaatcacaggagagcactccaagctaggactccctgtgcagccaatcagcaagcagttcttctagtgcctgcctgctggaacaggagtgccagcccagaagtgggtgcagcagcgccagaaaccagcaagtgcagcggataacggacccgccgtccctcctcgccaacccacattgggatcctcaaattcaggacagccagacacagacctccatgaatggaaatcggaaccgcaggacccgtgggggcacagcccttttttgtggtgcccagacggcctggctatggcaccatggggaagcccatcaaactgctggccaactgctttcaagtggagatcccaaagattgacgtctacctctatgaggtggacattaagccagacaaatgtccccgcagagttaacagggaggttgttgattccatggtccagcatttcaaagtcaccatatttggtgaccgtcgaccagtgtacgatggcaaaagaagcctgtacaccgccaatccactgcctgtagcatctactggggtggatttggatgtaacattgccaggagaaggagggaaagaccgtcccttcaaggtctccataaaatttgtctctaggataagctggcaccttttgcatgaagtacttactgggaggtcccttccagaaccgttggaactggacaagccaataagcaccaaccctgtccatgctgttgatgtagtgttacggcatctgccatccatgaagtacacccctgtaggacgttccttcttctctgctccagaaggctatgaccaccctctaggagggggaagagaagtctggttcggatttcatcagtctgttagacctgccatgtggaaaatgatgcttaatatagatgtttctgccactgccttctacaaagcacaaccagttattcagttcatgtgtgaagttctagatattcataacattgatgaacaaccccgacctctgaccgattctcaccgggtaaaatttaccaaagagataaagggtctgaaggttgaagtaactcattgtggaacaatgagacggaaataccgtgtttgcaatgtgacgaggaggcctgccagccatcaaaccttccccttacagctggagaatggacagactgtagagagaacagtcgctcaatactttagggaaaagtataatctccagctgaagtatcctcatttgccctgtctgcaagttggacaggaacagaaacacacatacctgcctctagaagtatgtaatattgtagctggccagcgctgcatcaagaagctgactgacaatcagacctccactatgatcaaagcaacagccaggtctgccccagacaggcaggaagagatcagcaggctggttagaagtgcaaattatgatgctgacccatttgttcaagaattccagtttaaagtgcgggacgaaatggctcatgtgacaggaagagtacttccagctccaatgttacaatatggagggcgaaatcgcacagtagccactccaagccacggggtttgggacatgcggggaaaacagtttcacactggtgtggagatcaaaatgtgggccatcgcatgctttgcaacgcagagacagtgcagagaagaaatcttgaaaggattcactgaccaattgagaaagatttcaaaagatgctgggatgcccatccagggccagccatgcttctgtaaatacgcgcagggcgcggacagcgtggagccaatgttcagacacctcaaaaatacatattctggtctccagctgattatagtcatactgccaggaaagacaccagtatatgccgaggtaaagcgagttggagacacattactgggaatggctacacagtgcgtccaagtaaagaatgtaataaaaacatctcctcaaactctctcaaatctgtgcctaaaaatcaacgtcaaactgggagggattaacaatatcttggtaccacatcaacggccgtctgtgttccaacaaccagtcattttcttaggcgcagacgtcactcatccacctgctggagatggcaagaaaccttcaattgctgctgttgtaggcagtatggatgcccacccaagtcgatactgtgcgacagtgcgggtacagagaccgcggcaggaaattatccatgaccttgcttccatggtgagagaacttcttatccaattctacaagtctacgcgcttcaagcccacacgcatcatcttttaccgtgacggagtttcggaaggacagttcagacaggtcttgtattatgagctgctagcaattagagaagcctgcattagtctggagaaggattaccagcctggaatcacctacattgtggtacagaagaggcatcatacaaggttgttctgtgcagatcgaactgaaagggttggcagaagtggaaatattccagctggtaccactgtggatacagatataacacatccttacgagtttgatttttacctctgcagccatgctggaatacagggtactagcaggccttctcactatcatgtcttgtgggatgataactgtttcactgctgatgaactccagctgctgacctaccagctgtgccacacatatgtgcgatgcacgcgatcagtctccatccctgcacctgcctattatgctcatcttgtggcattcagagctaggtaccatctcgtggacaaagaacatgacagtgctgaaggaagccacgtatctggacaaagcaatggccgggacccacaagcccttgctaaggccgtgcaaattcaccaggataccttacgtaccatgtacttcgcgtgagaccaactattgaccctaaaaatgcacaggtttgacttgcaacaaggaacctgatctcatgtgggttttcagggcccagtagagtggttcacattcgcctgccctcctctctcccaggccttttcttctggcttgcactgaagcccaggcccaatgcttgaggggagggctgatcctgggaatagaagatttttgtggaacacagcactattatgcaatatgaaaccagccaactgctagatcgttgtaatggagcctccacttgggtgcactgcttacgttttgtgtttcttttactttccaatattttaacccttttatgcctttacctcaaattgctctgcagcactagctgtctttgcaagaaaaaaaaaaagagaaaaaaagaaataccgtagggatgtatgggagtatgtttgcatatctgtacacacttgggctctgttttccggtacgtgggtatacagcataacgttctattctactcgctgtagtcattttacaaggctgtcagtataatattgtcttagctaccatgactaacccctgatctacttactgctgacctctcaacccattctgctgatatcggagtgactggtgaaactcctagatgcacaggtgtttaaatgtagaaaccagcccttgtgtgcccaggtatatttatatgtatcacacgtgtgtataattaattattggttacatatatgtgtatacatgcgtgtgcgactgtgtggatacgaagtaggtggatgttgtgcaaaaaaactaggatttctatttgtggcaccatctgtttcattttcaggtcccacttttttttgccttctatgtcaacctcattttcataggtcatgtacttcagagcttcccttttattcgttctgggacgttttagttaacaagatgcaagcaggtattgcatgtgccttgtactatggtcatgcaagtgactggatatatacttcagctattacaacaagaatattgccacttaccccctgcagtgcaacaacacatgttggcattgggataggaaaagcttagcacatggcccttcttgggtcttagtctagatactatggcctaatttctcaaactgtatctctcttgctatgtatacattttattcttgcgcaaaaagcaaggaaacacccctgaaaaatcccacatcccactcattaaactatttgttaactatccccttactcagtatgctgcaaactgttgtccatccccgtacattatgggagtgtcttaactttcaccctccaaataacattggaaactacttcaaaaggatgtgggaagacaccttctcttctagagtcatgtaacgtatacatggttg

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