LOCUS       XM_066409101            3913 bp    mRNA    linear   INV 16-JUL-2024
DEFINITION  PREDICTED: Branchiostoma lanceolatum protein argonaute-2-like
            (LOC136421635), mRNA.
ACCESSION   XM_066409101
VERSION     XM_066409101.1
DBLINK      BioProject: PRJNA1134169
KEYWORDS    RefSeq.
SOURCE      Branchiostoma lanceolatum (amphioxus)
  ORGANISM  Branchiostoma lanceolatum
            Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii;
            Amphioxiformes; Branchiostomatidae; Branchiostoma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089737) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_035083965.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/12/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Branchiostoma lanceolatum"
                     /mol_type="mRNA"
                     /isolate="klBraLanc5"
                     /db_xref="taxon:7740"
                     /chromosome="16"
                     /sex="female"
                     /tissue_type="whole body"
                     /dev_stage="adult"
                     /geo_loc_name="Italy"
                     /lat_lon="44.4137 N 8.9289 E"
                     /collection_date="2019-05-07"
                     /collected_by="Simona Candiani"
     gene            1..3913
                     /gene="LOC136421635"
                     /note="protein argonaute-2-like; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 28
                     Proteins"
                     /db_xref="GeneID:136421635"
     CDS             395..3046
                     /gene="LOC136421635"
                     /codon_start=1
                     /product="protein argonaute-2-like"
                     /protein_id="XP_066265198.1"
                     /db_xref="GeneID:136421635"
                     /translation="

MFPGFGQQQFPGHPPPPGPPGPPGPPGPVGFPAPVAPPVHSTPMDLFRCPRRPDVGTHGRRILLRANHFKVSMPKGDIHHYDVNIMPDKCPRRVNREIIEKMVQAYNRIFGDLRPVFDGRKNLYTRDPLPIGTEKVELDVTLPGDGGKDRHFKVSIKWVAKVSLYTLEQALEGRLAAIPFEAIQALDVVMRHLPSMTYTPVGRSFFSSPEGYSHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDINDQRRPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPAQTQTFPLQLEDGRTVECTVAKYFLERHKRKLEFPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREKEIIKLMQKANFNSDPYVRDFGINISTDMAEIEGRVLDPPMLQYGGRTRATVVPNQGVWDMRGKQFHTGIEIRVWAMACFAPQRQCSEQALRNFTQSLQRISNDAGMPILGQPCFCKYATGADQVEKMFRYLKNTFQGLQLILVVLPGKTPVYAEVKRVGDTLLGVATQCVQVKNVIKTSPQTLSNLCLKINVKLGGVNNILVPHIRPRVFREPVIFLGADVTHPPAGDEKKPSIAAVVGSMDAHPSRYAATVRIQTHRQEIIADLASMVRELLIQFYKSTRFKPTRIIMYRDGVSEGQFQQVLWHELRAIREACVRLEIGYEPGVTFIVVQKRHHTRLFCSDKKEQIGKSGNIPAGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNFAADDLQMLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKDHDSGEGSHRSGDSGERTPMDMARAVEVHPDTLRVMYFA"

     misc_feature    572..964
                     /gene="LOC136421635"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    992..1144
                     /gene="LOC136421635"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1145..1507
                     /gene="LOC136421635"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1301..1303,1346..1348,1388..1390,1400..1402,
                     1454..1456,1475..1477,1481..1483)
                     /gene="LOC136421635"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1640..2917
                     /gene="LOC136421635"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2051..2053,2063..2065,2099..2110,2117..2119,
                     2141..2143,2150..2152,2162..2164,2174..2176)
                     /gene="LOC136421635"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2255..2257,2261..2263,2471..2473,2885..2887)
                     /gene="LOC136421635"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ggctcttccaccattttggatttttgttggctacgaagcgagaagaggaggaagagaaatgaggaagatatagcgcacttcctccacgacttaagaagtcttccaagacatccgtgcattcctagaccaccatgggactaacttctagacatctcaagcggtgataagaaatctacagcgattttgaggagagtttcgaatcgtgcgagctcaaatatttttgcgggttattttaaaacaaggccgcccagctgttgttgttattgttgttttcttgatgtccgggtcttcaacttcaactttaagaaatcggacgggctgagaaggtttaagtcttcgtcgtgaggattttaaggattgggaacgagagcgggttccagcgggccagagcaccatgttcccagggttcggacagcaacagttcccgggccacccgccgccaccgggcccgcctgggccgcccggacctcccgggccagtcggttttcccgcgccggttgcgccgccagtacactcgactcccatggacctgttccgttgcccgcggcgacccgacgtcggcacccacgggcgtcggatcctcctcagagccaatcacttcaaggtgtcgatgccaaaaggagatattcaccactacgatgtcaacatcatgccggacaaatgtccaagaagagtcaacagagaaattatagaaaagatggtccaagcctacaaccgcatctttggtgaccttcgacctgtgttcgacgggcggaaaaatctgtacacacgagaccccctaccaataggaacagagaaggttgagctggatgtaacgttaccgggcgatgggggaaaagatcgtcacttcaaggtgtcaatcaagtgggttgccaaggtgagcctgtacacgctggagcaggcgctggagggccgtctggccgccatccccttcgaggccatccaggcactcgacgtggtaatgcgacacctgccctccatgacgtacacccctgtaggtcgctcgttcttctcctcacccgaaggttacagccacccgctgggcggaggtcgcgaggtctggttcgggttccaccagagtgtgcggccgtcccagtggaaaatgatgctcaacatagatgtttcagccacagcgttctacaaggcccagcctgttatagagttcatgtgtgaagttctcgacatcagagacatcaacgaccagagacgaccgctaaccgactcacaaagggtcaagttcaccaaggaaattaaaggcctgaaggtcgagatcacgcactgcggacagatgaagaggaagtaccgggtctgtaacgtcacgcggaggcccgcgcaaacacaaacgttcccgctgcagctggaggatgggaggacagtggagtgcaccgtggcaaaatacttcttagagagacacaagagaaaactagagtttccccaccttccctgtctacaagtcgggcaggaacagaaacacacctaccttcctttggaggtctgtaacattgttgccgggcaacgctgcatcaagaagctgacggacatgcagacctccaccatgatcaaggccaccgctcggtccgcacccgacagagaaaaggagatcatcaagttgatgcagaaagcgaacttcaactctgacccctacgtgcgagacttcggaatcaacatctcgacggacatggcggagatcgagggccgcgtgctggacccgcccatgctccagtacggaggcaggacacgagccacagtagtgccgaaccagggtgtgtgggacatgcgagggaagcagttccacaccggcatcgagatccgggtctgggccatggcctgcttcgctccacagaggcagtgcagcgagcaggcactaaggaatttcacgcagtccctgcagcgtatctcgaacgacgcggggatgccgatcctggggcagccgtgcttctgcaagtacgccacgggcgccgaccaggtggagaaaatgttccgatacctgaagaacaccttccagggcctgcagctcatcctggtcgtgctgccggggaaaacacctgtctacgccgaggtgaagagagtgggggacacgttgctaggcgtggccacgcagtgcgtccaggtgaagaacgtgatcaagacgtctccgcagacgctgtccaacctgtgcctcaagatcaacgtcaagctgggcggggtcaacaacatcctcgtcccgcacatacgcccgcgggtgttccgtgagccggtcatcttcctgggggctgacgtcacgcaccctccggcgggcgacgaaaagaaaccgtccatcgcagcggtggtcggcagcatggacgcccacccgagtcggtatgcggcgacggttcggatccagacccaccggcaggagatcatcgctgatctggcctccatggtgcgggaactgctcatccagttctacaagtccacccgcttcaagcccacccgcatcatcatgtaccgcgacggggtcagcgagggacagttccagcaggtgttatggcatgagctgcgggcgatccgtgaggcatgcgtgcgcctcgagatcggctatgaacccggcgtgaccttcatcgtggtgcagaaacgacatcacacaagactcttctgctctgacaagaaggaacagatcgggaagagcggaaacatcccagccggcaccactgtcgatgtcggcatcacacatcccacagagttcgacttctacctgtgcagccatgcaggcatacagggcacgagtcgaccgtcccactaccatgttctctgggacgacaacaactttgctgcggacgacctccagatgctgacgtaccagctgtgccacacgtacgtccgctgcacgcgcagcgtctcgattcccgcgcccgcctactacgcccacctggtcgccttccgcgctcgctatcatctcgtggagaaggaccacgacagcggggagggttcccatcggtccggagacagcggtgagcgcacgcccatggacatggcccgtgcagtcgaggtccaccccgacacgctacgcgtaatgtacttcgcgtagccacggcgaccgtaaccgtggcaaccgtagccatggcgactaaaactgcggcaaacgtagttgtggtgaccgtaactgcggcaaccgtagccctggcgaccgtaaccacggcgatccaaacagccagccgtacctcaacccaagtatttcccgcctcctgtctttcatatcaggccgttttgttaccatggaaaccagccaaatatttaaaaactaagaaaacttacaaagtgtgaagactgaagaacaaatgcgagactttccagaacgcttgcgaagaaaacaccaagtctttcagcggtgattgatgggcaactgagttgccactagagaacgaatttttatggtgtgttgttttgagaagtgtttgttgcgggtgtctgtagaggtgtagttataaggtgcttgctctgctgtatgttgtctgtatatctacagcacaaattaagaagccaccaaactacttttcatcgacggagtatctacagaatattgaattatctaattgtgtgcaaggaagcgaagagtaaactgctgttaggaatcggtggttttgttttagctagcgtctgtctgtactggttagtttgagccgagcatgtttgtacagaaagtctgtttactgcaatcaattcaagacttttatttatgttataagacacagagtgttctttagaagccaacaagatggtgcattcatttggtatatcagcctggcaaccctcaactcacctcaaaactggacctggcggctgtgctatggatagttttactctagctatgaatcctctggaataagtgacattgttgcggtcacactgacttgggtggtaggggcatgttgaatgagtcagtagctgcatcaac

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