LOCUS       XM_041706848            3000 bp    mRNA    linear   PLN 16-SEP-2025
DEFINITION  Aspergillus puulaauensis argonaute/piwi family protein
            (APUU_60047A), partial mRNA.
ACCESSION   XM_041706848
VERSION     XM_041706848.1
DBLINK      BioProject: PRJNA728012
            BioSample: SAMD00269938
            Sequence Read Archive: DRR262021, DRR262022
KEYWORDS    RefSeq.
SOURCE      Aspergillus puulaauensis
  ORGANISM  Aspergillus puulaauensis
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
REFERENCE   1
  AUTHORS   Futagami,T., Mori,K., Kadooka,C. and Tanaka,T.
  TITLE     Aspergillus puulaauensis MK2 genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2025) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 3000)
  AUTHORS   Kazuki,M. and Futagami,T.
  CONSRTM   Aspergillus puulaauensis MK2 genome sequencing consortium
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2021) Contact:Taiki Futagami Kagoshima
            University, Agriculture; Korimoto 1-21-24, Kagoshima, Kagoshima
            890-0065, Japan
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NC_054862).
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Aspergillus puulaauensis"
                     /mol_type="mRNA"
                     /strain="MK2"
                     /db_xref="taxon:1220207"
                     /chromosome="6"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
     gene            <1..>3000
                     /locus_tag="APUU_60047A"
                     /db_xref="GeneID:64977004"
     CDS             1..3000
                     /locus_tag="APUU_60047A"
                     /note="COG:J;
                     EggNog:ENOG410PISF;
                     InterPro:IPR012337,IPR032474,IPR036397,IPR014811,
                     IPR036085,IPR003100,IPR003165;
                     PFAM:PF02171,PF16486,PF08699;
                     antiSMASH:Cluster_6.2;
                     go_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     go_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /product="argonaute/piwi family protein"
                     /protein_id="XP_041559193.1"
                     /db_xref="GeneID:64977004"
                     /translation="

MSFKGFRGPKSQASGGPGGHGQGGRGQGGRGQGGRGQGGPGRGQGDRAGPSIFSPGYDAAPDTGVADTENALIKSFTGPSSTPIDPMMPRRPGFGTRGQKVVLWANSFPLAFKEDVRLYRYNVDITPGTGAANVPTGKKKRRVVQLLLDNHFSGHRLGIATDFKSTLISATDLELGEEVYQVPYRGDFEDEHAAGAPTYNVRVQSTGMVNLPALIDYLTSTNAGAMLGSKDEVIQALNIVLGHTPRAAFDMASIGSSKHFRLTGGTGEKLDIGSGLCALRGFFFSVRAATARAIVNVQVKSAAFYNEGPLDMVMRAYLQDNGHSRVKLGNFLKRLSVNVTHIKRTNRQGQVVPRVKQIFGLATQQDGQGDPTPPKVSSFGAGPKDVLFYLGDPSSTEKSKKGKKAEKGGPYPPVSKNGYISVYDFFRQRYQITIKDPSLPVINTGTRQRPSYLPAQTCFVLPGQPSKAKLSPMQTQNMIKFAVRKPVMNAESIVTSGLQTLGVGGEASNLGTLGMNLTPRLLAVPGRVLNSPDVKYGGNGMVRPTSGSWNMSSMKFSAASQLTFWTFLWLSYPGGRDPWKDNAALKPTVDAFTDTLRQTGINVNDCTLGQHIVVNDVDQDIDTAVKKFTASPKPPLFILVILPAADTAIYNRVKYVCDVKEGLPNICVVANKFAKPRNDQYFSNIALKLNLKLGGRNQLLDAARLGIVSEGKTMVVGLDVTHPSPGSSPDAPSVAAIVASVDKSLGQWPADLRIQPARQEMVASLDLMLRSRLDLWKKHNGSYPANILVYRDGVSEGQYNLVLENELPALRKACRELYSPDSTKKGIPRLTIVVVGKRHHHRFYPSRAEDADKSTNPRNGTVVDRIVTEARHWDFFLQAHAAIQGTARPAHYYIIYDEIFRDTNVRPPFKNAADALEDLTHNMCYLFGRATKAVSICPPAYYADLVCERARCYLSRLFDPSPVVSPEPSVMGGAGGVSMTPDSSLVAIHDRIRHTMFYI"

     misc_feature    424..723
                     /locus_tag="APUU_60047A"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    763..915
                     /locus_tag="APUU_60047A"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    919..1383
                     /locus_tag="APUU_60047A"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1063..1065,1162..1164,1264..1266,1276..1278,
                     1330..1332,1351..1353,1357..1359)
                     /locus_tag="APUU_60047A"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    <1237..1437
                     /locus_tag="APUU_60047A"
                     /note="PAZ domain; Region: PAZ; pfam02170"
                     /db_xref="CDD:460472"
     misc_feature    1525..2862
                     /locus_tag="APUU_60047A"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1948..1950,1960..1962,1996..2007,2014..2016,
                     2041..2043,2050..2052,2062..2064,2074..2076)
                     /locus_tag="APUU_60047A"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2155..2157,2161..2163,2374..2376,2830..2832)
                     /locus_tag="APUU_60047A"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

atgtcgttcaaggggtttcgcggtccaaagtctcaagctagcggtggcccgggaggccatggacagggaggccgtggacagggaggtcgtggacagggaggtcgtggacagggaggtccaggtcgtggtcaaggcgatcgagctggcccgtcaatcttcagccccggctacgatgctgctccggatacaggggttgctgatactgagaacgctctcatcaagtccttcacggggcctagcagtactccgatagaccccatgatgccgcggcgcccagggttcggtacccgaggacagaaggtggtgctgtgggctaattcgttcccgttggcattcaaggaagatgttcgcctttaccggtacaatgtcgatatcacaccaggcactggtgcagcaaatgttccgacagggaagaagaagaggcgggtggtccagttactcctcgacaaccatttctctggtcacagacttggtattgcgacggattttaaatctactctcataagcgcaacggatcttgagctgggcgaggaagtgtatcaggtcccgtatcgcggcgatttcgaggacgagcatgcggctggtgcaccaacttacaatgtccgcgtgcaatcaacgggcatggtgaatttgcctgcattgatcgactatctgacatccaccaatgctggcgcaatgctaggttcgaaagatgaagtcatacaagccctgaatatcgttcttgggcacactcccagggcagcttttgatatggcatcaatcggctctagcaagcactttcgtctcacgggcggtaccggtgagaagctggacattggcagtggattatgtgctcttcgcgggttcttcttcagcgtccgtgcagctactgcccgggccatagtcaacgttcaagttaaaagcgcggcgttttacaatgaagggcctctggacatggtgatgcgggcgtatctacaagacaacggacatagtagggttaaacttggcaattttctcaaacgactatcggtcaacgtgacacatatcaagagaacaaatagacaaggccaggtggtcccacgagttaaacagatttttggcctggctactcagcaagacggccagggtgacccaactcctccaaaggtcagttcgtttggcgcagggccaaaggacgtcctcttttaccttggcgaccctagtagtaccgaaaagtccaagaaggggaagaaggctgaaaaaggtggaccttatcctcctgtgtccaaaaatggatatatcagtgtatacgactttttccggcaaaggtatcagatcaccatcaaagacccaagtcttccagttatcaacacgggaactcgccagcgaccatcctacctccccgcccagacgtgctttgttcttcctggtcagccttcgaaggctaaattgagtccgatgcagacccagaatatgatcaagtttgcagtccgcaagcccgtaatgaacgcggaatcgattgtcacgtctgggcttcaaacactcggtgtagggggcgaggcttcgaatctgggcaccttgggaatgaatttgactccgagattacttgcggtgcctggacgtgtgttgaatagccccgacgtcaaatatggagggaatggaatggtaaggccaacgtctggcagctggaatatgtcgtccatgaagttctccgccgcctcccaactaacgttttggactttcttgtggttgtcgtatcctggcggcagggacccatggaaagacaatgctgccctgaagcctacagtcgatgcctttaccgatactcttcgccagaccggcattaacgtcaatgactgtactctgggccagcacatcgttgtgaatgacgtggaccaggatatcgacacagcggtcaaaaagtttaccgctagcccaaagcctcccctttttattcttgtgattcttccagcggccgataccgccatttacaaccgagtgaaatatgtctgcgatgtcaaggagggcctgccaaacatctgtgtcgttgctaataaatttgcaaaacccagaaatgaccaatacttctccaatatcgccctcaagttgaacctcaaacttggcggccggaaccagctcctggatgctgccagacttggaattgtatccgaaggcaagactatggtagtcggcctggacgtgacgcatccgtctccaggctcgtctcctgacgcacccagtgtggcagccattgttgcttctgtggataagagtcttggacagtggcctgcggacttgagaattcagccagcgcggcaggaaatggtagcgagtttggacctcatgctgagatcacggctggatctctggaagaaacataatgggtcgtatccagccaatatcctggtttacagagacggcgtatcggaaggccaatacaatctggtgcttgagaacgagctgcccgcgctgcgcaaggcctgcagagagctgtactcgccggactctacaaagaagggcattccccggttgacaattgtcgtcgtcgggaagcgtcaccatcaccgcttctacccctcgagggcagaggacgccgacaagtcaacaaacccgcggaacggaacagtggttgaccgcatcgtcaccgaagcacgtcactgggacttcttcctccaagcacacgcggcaatccaaggcaccgcccgtccggcacactactacattatctatgatgagattttccgcgacacgaacgtccggccgccgttcaaaaacgcagccgacgccctggaggacctcacacataatatgtgctatctgtttgggcgtgctaccaaggctgtcagtatctgtccgccagcctactacgctgacctcgtttgcgagagagcgcggtgctatctgagcaggcttttcgacccatcaccggtagttagtccggagcccagtgtaatgggcggtgcaggtggagtatcgatgaccccggattccagtctcgtggccatccacgacagaattcgccacacgatgttctatatctaa

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