LOCUS       XM_026301804            3928 bp    mRNA    linear   VRT 08-JUL-2025
DEFINITION  PREDICTED: Mastacembelus armatus NRDE-2, necessary for RNA
            interference, domain containing (nrde2), transcript variant X1,
            mRNA.
ACCESSION   XM_026301804
VERSION     XM_026301804.1
DBLINK      BioProject: PRJNA489256
KEYWORDS    RefSeq.
SOURCE      Mastacembelus armatus (zig-zag eel)
  ORGANISM  Mastacembelus armatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Anabantaria; Synbranchiformes; Mastacembelidae;
            Mastacembelus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_046654.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900324485.2-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/03/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Mastacembelus armatus"
                     /mol_type="mRNA"
                     /db_xref="taxon:205130"
                     /chromosome="22"
     gene            1..3928
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 5 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 6 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:113127328"
     CDS             53..3658
                     /gene="nrde2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform X1"
                     /protein_id="XP_026157589.1"
                     /db_xref="GeneID:113127328"
                     /translation="

MRHIIMALFPAFAGLADNKVDDPSEELDWLNNKSFQARDALSLHSRFIEEKESSVGREVGSAEEEKEGEDNVPLKKKKKKSEKKRKKKKKHKKKSGRDSDSGGSDSDTIYPSDLKKEHEADRTQAAPLASRFSWLDDLHLPLEQPFCVDHKPDPANWTYKSLYRGDVARYKRKGSSSLGLDRRRQEVSWEESESRKKQKDKKKTADRYFSAVSRQLLRSEIPVPTLPVIPECGDVIKYPSFLQLSEDAEKKGGEAGDKVQMSWVNPLGVYDPSTALWLQGKGQDKQKEDLQAGQSAVLLAGRTEEFNRRLREQPADTQLWIQFIQYQDELTSAMFGGEEEQQGNDSTEWRKSSYRAVLEKKLSIAERAVATNPGSVALQLERLRICQELWEPSVLAKEWKKLVFLHPNSTSLWRAYLLFTQSYFSNFTVSKVNSAYGKCLSTLSAVRDGTMVSHPALPGIEEDMLDIFTQQCHFLRQSGHTEKAISLFQAMMDFTFYKPDSVRELSTKQQVEFFEPFWDSGEARVGELGARGWKAWMVQQERGGWLQPSAEEEEEEEDEEEEVKDRRQPRWTVWLDVESSREAAQWLPWRPDKSKGQSEEDCEDPDRQVLFDDIGPSLICLSSPELQLQLLLHFMSFLGLPVDSALFAAPSQPGLLLENLSLLTQGIDPLRPLTSHDLPDTGLNAVGHMTTLQGTKKWAGLGKQGETFITNVFNMVQAVLPVHHQAILSLSWIQYEKLKVLRCLRSGNKKRLRSQGKSSKRVAKRLLKESNNRSSLVLWREYAHLEWMLGNVDEARKVFSTATAMGGTKGLSSPSLCDLCLLWAQLEVENGVAVRGADLTDVTASPAVTVLTKLAEGTSTFSSSSDPLSPVSVLKARRSYEQILTASLAALDKDPNNSQTNIKGQEHLLEEKLRLKGLVGCYALFQYLTVGIQAANAVYSQARERIEEQYHTLTLDKQPNSNEGANLASTDSSNSAADSCYTSRNYMKRLASECEVLAVQQAALLRYHNSVSVFPLATLRQTLTSTLSTWCSSAPLWSIYVQVEDRYHSAGRARRFFHSVTRHTDSVVPRLFAIVVEQQRKQLVDAAQKSCCHDAALPILPENGLSNRIRGLFETAIATEIGAHCPLVWRMYMHFLVSEGKVDKATGIFYRALQNIPWAKGLYMDAVQLFPEHLQEFVDLMTEKELRLRLPLEELDILLED"

     misc_feature    485..745
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(485..511,518..523,533..538,542..544,548..592,
                     599..607,611..619,647..655,665..670,674..679,686..706,
                     737..745)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    965..1969
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

ttttttgttttttagcttaaactgacgacgtcatcttcccgcgactgtttttatgcgacatatcatcatggcgctgtttccagcttttgctggattagcagacaacaaagttgacgatccgtccgaagaattagactggctgaacaataagagctttcaggccagggacgccctctctcttcacagtcggtttatagaggaaaaggaatcaagtgtaggtagagaggtgggctctgcggaggaagagaaggaaggagaggacaatgtgccactcaaaaagaagaaaaagaaaagtgaaaagaaacgaaaaaagaagaaaaaacataaaaagaagagtggaagggattcggacagtggtgggtctgactctgacaccatctaccccagtgacctcaaaaaggaacatgaggcagataggacccaggccgctcccttagcaagtcgtttctcctggttggatgacctccacctgcccctggagcagccgttctgtgtggaccataaacctgatccagccaactggacatacaagtcactgtacagaggagacgtagcaaggtacaagaggaaaggcagctcctcactgggtctggaccgtcgcaggcaggaagtcagttgggaggaatcagaatcaagaaagaaacagaaagacaaaaagaaaacagcagacagatacttctctgcagtcagtcggcagctgctgaggtctgagattcctgttcctactttaccagtgatccctgagtgtggtgatgtcattaaatacccatcattcctccaattgagtgaggatgcggaaaagaaaggaggagaggcaggtgacaaagtgcagatgtcatgggtaaatcctctcggtgtgtacgacccctccacagccctttggctgcagggcaaaggacaggacaagcagaaggaggacctacaggctgggcagagtgctgtgctgttggccggcaggactgaagagtttaacagacggctgagagagcagccagcagacacacagctgtggatacagtttatacagtatcaggatgagctgacttcagccatgtttggaggggaggaggaacagcagggcaatgattcaactgagtggcgcaagtcttcctaccgagcggttctagagaaaaagctgagcatcgctgagcgtgctgtggccaccaaccctggctccgtagctctgcagctggagagactcaggatctgccaggagctctgggagccctcagttctggctaaagagtggaagaaattggtgttcctccacccaaacagcacctccctgtggagggcgtatctgcttttcacccagagctacttcagcaacttcactgtgtcaaaagtcaactctgcctatgggaagtgtctgagcacgctcagtgctgtgagggatggcaccatggtctctcaccccgcgttaccagggattgaggaggacatgttggatatcttcacccagcagtgtcatttcctgcgtcagtctggtcacacagagaaggcgatttctctctttcaggccatgatggactttactttttacaaacccgacagtgtacgagagctgtccaccaagcagcaggttgaattctttgaaccattctgggacagcggagaggcaagggttggagagttgggggccagaggctggaaagcctggatggtccaacaagagaggggggggtggctacagcccagtgcagaggaagaagaagaggaggaggatgaggaggaggaggtgaaggatcggagacagcccagatggactgtctggctggatgtggagtcatctcgcgaagcagctcagtggctgccctggaggcctgacaaatccaagggccagtcagaagaggactgtgaagacccggacagacaggtgctatttgacgacatcggcccatccctaatttgcctgtcatcacccgagcttcagctgcagcttcttctccatttcatgtcgttcctagggctgcctgtggactctgcgctctttgctgcaccttctcagcctggcctgctgctagagaacctttcactgctgactcagggtattgacccgctgcgtcctctgacctcccatgacctacctgacactgggcttaacgctgtaggtcacatgacaactctccaaggaacaaagaagtgggcagggctgggaaagcagggtgagacgtttatcacaaatgtgttcaatatggtccaagccgtccttcctgtgcatcaccaggctatcctgtcactcagctggattcagtatgagaaacttaaggtcttgcgctgcctacgcagtggcaacaaaaagcgtctccgctctcagggcaagagcagcaagcgggtggccaagcgactgctcaaagaatccaataaccgctcgtcattggtgctgtggcgagagtatgctcacctggagtggatgctgggcaacgttgacgaagctcgcaaagtgttctctacagctacagcaatggggggaaccaaagggctaagtagccccagtctctgcgacctttgtctgctgtgggcacagctggaggtggagaacggagtggctgtgcgaggcgcagatctaactgatgtaactgcttccccagctgtcacagtgctaaccaagttagctgaaggaacctccacattctcttcatcctccgaccccctctctccagtctctgtcctgaaagccaggaggtcatatgaacagattctaacagccagtttggcagcactggataaggaccctaacaactctcagaccaacataaaaggtcaagagcacctgcttgaggagaagctgaggttgaaaggcctggttggctgctatgctctgttccagtacctcacagtgggcatccaagcagctaacgccgtctatagccaggctagagagaggatagaggagcagtatcacacattaacactggataagcagcccaacagtaatgagggagccaaccttgctagcaccgattccagcaactctgcagctgattcctgctacaccagcaggaactatatgaagaggctggcttctgagtgtgaagtattagcagtacagcaggcagctttgctgaggtaccacaacagtgtcagtgtgttccccttggcaacactaagacaaactctgacttctaccctctccacctggtgtagcagtgcacctctctggagtatatatgtacaggttgaggaccgttaccatagtgctggccgagcacgccgcttttttcattctgtaactaggcacaccgacagtgtggtgccacgcctttttgccattgttgtcgaacaacaaaggaagcagctggtggacgctgctcagaagtcttgttgccatgacgctgccttgcccatccttccagagaatggcctcagcaaccgtatccgtggactgtttgaaactgccatagcaacagagataggtgctcactgtcctttagtttggaggatgtacatgcatttcctggtgtctgaagggaaggtggataaagccacagggatcttctacagggccttacagaatattccctgggctaagggcttgtacatggatgcagtgcagctgttccctgagcatctacaggagtttgttgatctgatgacagagaaagaactccgtctcagactgcctttagaagaactggatatactactggaagactgaacacacattcaagggctcactttgagtttgcttaagtggcaatatgacacctcacatattagcacacttaaagactacaggagatacacacctgagttcagatttatccacatataccctagctgttgtattaaacagtgatgttgcagtgatcttatgtaaagatgagcagagaagctacccttttgacatattgtctgaaatgctttattttaacaatataaatttgtttcagtacctgagatcttcaataaatgtaattctcattta

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