LOCUS       NM_138356               2517 bp    mRNA    linear   PRI 17-APR-2013
DEFINITION  Homo sapiens Src homology 2 domain containing F (SHF), mRNA.
ACCESSION   NM_138356 XM_032304
VERSION     NM_138356.2  GI:111074523
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Lindholm,C.K., Frantz,J.D., Shoelson,S.E. and Welsh,M.
  TITLE     Shf, a Shb-like adapter protein, is involved in PDGF-alpha-receptor
            regulation of apoptosis
  JOURNAL   Biochem. Biophys. Res. Commun. 278 (3), 537-543 (2000)
   PUBMED   11095946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK096267.1, CN287519.1, BX416415.2 and BM669861.1.
            On Aug 2, 2006 this sequence version replaced gi:46195760.
            
            CCDS Note: The coding region has been updated to extend both the N-
            and C-termini based on available transcript data. The update is
            based on the splice pattern of AK096267.1, a full-length mRNA. The
            same protein is described in PMID:11095946, but the published
            sequence is over-extended by 57 aa at the N-terminus due to the
            presence of an uncorrected 5' mismatch compared to the reference
            genome sequence.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK096267.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025084, ERS025088 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               AK096267.1         1-346
            347-685             CN287519.1         219-557
            686-1502            AK096267.1         686-1502
            1503-2208           BX416415.2         115-820             c
            2209-2517           BM669861.1         1-309               c
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..2517
                     /gene="SHF"
                     /note="Src homology 2 domain containing F"
                     /db_xref="GeneID:90525"
                     /db_xref="HGNC:25116"
     exon            1..449
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    439..441
                     /gene="SHF"
                     /note="upstream in-frame stop codon"
     exon            450..798
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     CDS             496..1767
                     /gene="SHF"
                     /codon_start=1
                     /product="SH2 domain-containing adapter protein F"
                     /protein_id="NP_612365.2"
                     /db_xref="GI:111074524"
                     /db_xref="CCDS:CCDS10120.2"
                     /db_xref="GeneID:90525"
                     /db_xref="HGNC:25116"
                     /translation="

MQQEGGPVRSAPCRTGTLEGSRQGSPGHRKRASPKGSLSSAQPHSWMLTPSPLNSHCAHREPISSSPQPVANGPKQKKKSNWRSTTRLRIIRLRDRLEPRPLAILEDYADPFDVQETGEGSAGASGAPEKVPENDGYMEPYEAQKMMAEIRGSKETATQPLPLYDTPYEPEEDGATPEGEGAPWPRESRLPEDDERPPEEYDQPWEWKKERISKAFAVDIKVIKDLPWPPPVGQLDSSPSLPDGDRDISGPASPLPEPSLEDSSAQFEGPEKSCLSPGREEKGRLPPRLSAGNPKSAKPLSMEPSSPLGEWTDPALPLENQVWYHGAISRTDAENLLRLCKEASYLVRNSETSKNDFSLSLKSSQGFMHMKLSRTKEHKYVLGQNSPPFSSVPEIVHHYASRKLPIKGAEHMSLLYPVAIRTL

"
     misc_feature    1456..1749
                     /gene="SHF"
                     /note="Src homology 2 domain found in SH2
                     domain-containing adapter protein F (SHF); Region:
                     SH2_SHF; cd10392"
                     /db_xref="CDD:198255"
     misc_feature    order(1483..1485,1537..1539,1600..1602,1606..1608)
                     /gene="SHF"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]; other site"
                     /db_xref="CDD:198255"
     misc_feature    order(1603..1605,1690..1692)
                     /gene="SHF"
                     /note="hydrophobic binding pocket [polypeptide binding];
                     other site"
                     /db_xref="CDD:198255"
     exon            799..940
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     exon            941..1147
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     exon            1148..1288
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     exon            1289..1460
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     exon            1461..1580
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     exon            1581..2500
                     /gene="SHF"
                     /inference="alignment:Splign:1.39.8"
     variation       2348
                     /gene="SHF"
                     /replace="c"
                     /replace="t"
                     /db_xref="dbSNP:1629906"
     STS             2354..2485
                     /gene="SHF"
                     /standard_name="RH66736"
                     /db_xref="UniSTS:53437"
ORIGIN      

gtactaaccactatacgatcacggcgagctaccgggacgccacgacgagcagctgacccagtgttgcccttctcgtctagatggcggccattccagctttcctaagcgtccattatctaccaggttcctcccgcacccacgttcacccgcaagagcagccgggagcgctgtggactccctagcccgaagtctcctcttggctgtacgcacggaccgcaaaagaagggacatgaaagtgctttgcaaccaaaggattcctatgaggccgggcctgcaatgatgtgaagcccctgaggacgcccgaatggtggcccaaagtctctctgacgctcaccatgccctgactatcatgctatctgaacgactttggctgggactggagctcttaaattttacaagaaggaaaatcagttctgacccaccaccatacaacttccttgaggcgcgagggtgtccacaatctccacagaggcggcattgggtcaccgctggaggatgcagcaggagggaggacccgtgaggagcgccccctgtaggaccggaaccctggagggttcgcggcaagggtctccggggcataggaaacgggcatcccccaagggtagtctgagttccgcccagccccattcctggatgctcacgccgtctcccctaaactcccactgcgctcaccgggagcccatctcatcatcaccacagcccgtggctaatgggccaaaacagaagaagaaaagcaactggcggagtacaacgcgcctgcgcattatcagactcagggacaggctggaacctcgccccctagctatcctagaagactatgcggacccgtttgatgttcaggagactggcgaaggctcagcaggagcttcaggagccccagagaaggtccctgaaaatgatggctacatggagccctatgaggctcaaaagatgatggccgagatccggggctccaaggagacagcaactcagcccttgcctctgtatgacacaccctatgagccagaggaggatggggccaccccggaaggtgagggggccccctggccccgggagtcccgcctgccagaggatgatgagaggccccctgaggagtatgaccagccctgggagtggaagaaggagcggatttccaaagcctttgcagttgacattaaggtcatcaaagacctaccttggcctccacctgtgggacagctggacagcagcccctccctgcctgatggggacagggacatctccggtccagcctcgcccctccctgagcccagcctggaggacagcagcgcccagtttgaaggaccggagaagagctgcctgtcacctggccgggaggagaaggggcggctacctccccgactctctgcagggaaccccaagtcagccaaacccctaagcatggagcccagcagccccctgggggagtggacagatccagcactgcctctggaaaaccaggtctggtatcacggggccatcagccgaaccgacgccgagaacctgctccggctgtgcaaagaggccagctacctggtgcgcaacagtgagaccagcaagaatgacttctccctctccctcaagagcagccagggattcatgcacatgaagctgtcccgaaccaaggaacacaaatatgtgctgggccagaacagcccgcccttcagcagcgtccctgaaattgtgcaccactatgccagccgcaagctacccattaagggagccgaacacatgtccctgctctaccctgtggccatccggactctttagatgtgaagccagggcactgtgatagacctgtacccagccctgtgcccatcacctggctgagggctgtggctcttgccagggacgtgatctttcaaacctttcttctcctgggatccagtagaagctggagattccttaatttattctaaagggaaagggctcctggggccttggagtaaggggttgtctggagctggggaaagaggaatccctggagagaaaggatagcccctggaggaagggggttccagagctactgggatggtagggagtttcagactggcagctccggctcctttccgaccttagggcagaggtggtgacctccaccaccaccaccctctccccactgggtccgtgcgaggtagtgcagaattcggccccttggggcgccttaccacctctctgcctccgtccccgacttccaccccagaccgtcggagggctccgcccagagtctggtaagaggtttggggaagacaggcccctgggaagcagccggctttggggggtggggagagaaggggaggggctcgggcagagggaactgtgcagtccccaggccgccccggctccgggccagaggcaataaataaacccgatcctgccgggcacagccgcgcccgcgcctccggcgccgtccccgggctgacgggggagggagcggagaagcgagcgcagattctgcgtataaatcagctctggagcagacacagcccggctgtgaaaagcaaaaaaaaaaaaaaaaaa

//

ANNOTATIONS from NCBI Entrez Gene (20130726):
            GeneID:90525 -> Biological process: GO:0006915 [apoptotic process] evidence: IEA