GGRNA | NM_015339.2

LOCUS       NM_015339               4713 bp    mRNA    linear   PRI 17-APR-2013
DEFINITION  Homo sapiens activity-dependent neuroprotector homeobox (ADNP),
            transcript variant 1, mRNA.
ACCESSION   NM_015339
VERSION     NM_015339.2  GI:31563504
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Yang,M.H., Yang,Y.H., Lu,C.Y., Jong,S.B., Chen,L.J., Lin,Y.F.,
            Wu,S.J., Chu,P.Y., Chung,T.W. and Tyan,Y.C.
  TITLE     Activity-dependent neuroprotector homeobox protein: A candidate
            protein identified in serum as diagnostic biomarker for Alzheimer's
            disease
  JOURNAL   J Proteomics 75 (12), 3617-3629 (2012)
   PUBMED   22554909
  REMARK    GeneRIF: Our results suggested that ADNP may play an important role
            in slowing the progression of clinical symptoms of AD.
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Aboonq,M.S., Vasiliou,S.A., Haddley,K., Quinn,J.P. and Bubb,V.J.
  TITLE     Activity-dependent neuroprotective protein modulates its own gene
            expression
  JOURNAL   J. Mol. Neurosci. 46 (1), 33-39 (2012)
   PUBMED   21647709
  REMARK    GeneRIF: Chromatin immunoprecipitation demonstrates the ability of
            ADNP to bind to its own promoter, consistent with its action as a
            repressor of both promoter-supported and endogenous ADNP
            expression.
REFERENCE   3  (bases 1 to 4713)
  AUTHORS   Dresner,E., Agam,G. and Gozes,I.
  TITLE     Activity-dependent neuroprotective protein (ADNP) expression level
            is correlated with the expression of the sister protein ADNP2:
            deregulation in schizophrenia
  JOURNAL   Eur Neuropsychopharmacol 21 (5), 355-361 (2011)
   PUBMED   20598862
  REMARK    GeneRIF: In the prefrontal cortex of schizophrenia patients the
            correlation between ADNP and ADNP2 mRNA levels was apparently
            higher than in the hippocampus (r=0.854, p<0.001), but did not
            reach a significant difference (p=0.25).
REFERENCE   4  (bases 1 to 4713)
  AUTHORS   Braitch,M., Kawabe,K., Nyirenda,M., Gilles,L.J., Robins,R.A.,
            Gran,B., Murphy,S., Showe,L. and Constantinescu,C.S.
  TITLE     Expression of activity-dependent neuroprotective protein in the
            immune system: possible functions and relevance to multiple
            sclerosis
  JOURNAL   Neuroimmunomodulation 17 (2), 120-125 (2010)
   PUBMED   19923857
  REMARK    GeneRIF: ADNP is expressed in many immune system cells. ADNP mRNA
            is reduced in PBMCs in MS. The peptide NAP, which plays an
            important role in neuroprotection, has potential immunomodulatory
            properties.
REFERENCE   5  (bases 1 to 4713)
  AUTHORS   Mandel,S. and Gozes,I.
  TITLE     Activity-dependent neuroprotective protein constitutes a novel
            element in the SWI/SNF chromatin remodeling complex
  JOURNAL   J. Biol. Chem. 282 (47), 34448-34456 (2007)
   PUBMED   17878164
  REMARK    GeneRIF: Activity-dependent neuroprotective protein constitutes a
            novel element in the SWI/SNF chromatin remodeling complex.
REFERENCE   6  (bases 1 to 4713)
  AUTHORS   Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
            Jurisica,I. and Li,S.S.
  TITLE     Systematic identification of SH3 domain-mediated human
            protein-protein interactions by peptide array target screening
  JOURNAL   Proteomics 7 (11), 1775-1785 (2007)
   PUBMED   17474147
REFERENCE   7  (bases 1 to 4713)
  AUTHORS   Kankova,K., Stejskalova,A., Pacal,L., Tschoplova,S., Hertlova,M.,
            Krusova,D., Izakovicova-Holla,L., Beranek,M., Vasku,A., Barral,S.
            and Ott,J.
  TITLE     Genetic risk factors for diabetic nephropathy on chromosomes 6p and
            7q identified by the set-association approach
  JOURNAL   Diabetologia 50 (5), 990-999 (2007)
   PUBMED   17345061
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   8  (bases 1 to 4713)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 4713)
  AUTHORS   Zamostiano,R., Pinhasov,A., Gelber,E., Steingart,R.A., Seroussi,E.,
            Giladi,E., Bassan,M., Wollman,Y., Eyre,H.J., Mulley,J.C.,
            Brenneman,D.E. and Gozes,I.
  TITLE     Cloning and characterization of the human activity-dependent
            neuroprotective protein
  JOURNAL   J. Biol. Chem. 276 (1), 708-714 (2001)
   PUBMED   11013255
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF250860.1.
            On Jun 10, 2003 this sequence version replaced gi:12229216.
            
            Summary: Vasoactive intestinal peptide is a neuroprotective factor
            that has a stimulatory effect on the growth of some tumor cells and
            an inhibitory effect on others. This gene encodes a protein that is
            upregulated by vasoactive intestinal peptide and may be involved in
            its stimulatory effect on certain tumor cells. The encoded protein
            contains one homeobox and nine zinc finger domains, suggesting that
            it functions as a transcription factor. This gene is also
            upregulated in normal proliferative tissues. Finally, the encoded
            protein may increase the viability of certain cell types through
            modulation of p53 activity. Alternatively spliced transcript
            variants encoding the same protein have been described. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF250860.1, BC075794.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025084 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..4713
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="activity-dependent neuroprotector homeobox"
                     /db_xref="GeneID:23394"
                     /db_xref="HGNC:15766"
                     /db_xref="HPRD:09793"
                     /db_xref="MIM:611386"
     exon            1..165
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /inference="alignment:Splign:1.39.8"
     exon            166..340
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    238..240
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="upstream in-frame stop codon"
     exon            341..453
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /inference="alignment:Splign:1.39.8"
     CDS             346..3654
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="ADNP homeobox 1; activity-dependent neuroprotective
                     protein"
                     /codon_start=1
                     /product="activity-dependent neuroprotector homeobox
                     protein"
                     /protein_id="NP_056154.1"
                     /db_xref="GI:12229217"
                     /db_xref="CCDS:CCDS13433.1"
                     /db_xref="GeneID:23394"
                     /db_xref="HGNC:15766"
                     /db_xref="HPRD:09793"
                     /db_xref="MIM:611386"
                     /translation="

MFQLPVNNLGSLRKARKTVKKILSDIGLEYCKEHIEDFKQFEPNDFYLKNTTWEDVGLWDPSLTKNQDYRTKPFCCSACPFSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTFNADKKTLETHIKIFHAPNASAPSSSLSTFKDKNKNDGLKPKQADSVEQAVYYCKKCTYRDPLYEIVRKHIYREHFQHVAAPYIAKAGEKSLNGAVPLGSNAREESSIHCKRCLFMPKSYEALVQHVIEDHERIGYQVTAMIGHTNVVVPRSKPLMLIAPKPQDKKSMGLPPRIGSLASGNVRSLPSQQMVNRLSIPKPNLNSTGVNMMSSVHLQQNNYGVKSVGQGYSVGQSMRLGLGGNAPVSIPQQSQSVKQLLPSGNGRSYGLGSEQRSQAPARYSLQSANASSLSSGQLKSPSLSQSQASRVLGQSSSKPAAAATGPPPGNTSSTQKWKICTICNELFPENVYSVHFEKEHKAEKVPAVANYIMKIHNFTSKCLYCNRYLPTDTLLNHMLIHGLSCPYCRSTFNDVEKMAAHMRMVHIDEEMGPKTDSTLSFDLTLQQGSHTNIHLLVTTYNLRDAPAESVAYHAQNNPPVPPKPQPKVQEKADIPVKSSPQAAVPYKKDVGKTLCPLCFSILKGPISDALAHHLRERHQVIQTVHPVEKKLTYKCIHCLGVYTSNMTASTITLHLVHCRGVGKTQNGQDKTNAPSRLNQSPSLAPVKRTYEQMEFPLLKKRKLDDDSDSPSFFEEKPEEPVVLALDPKGHEDDSYEARKSFLTKYFNKQPYPTRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDFDAEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNASENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA

"
     misc_feature    637..639
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    637..639
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="phosphorylation site"
     misc_feature    670..732
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="C2H2-type zinc-finger domain; Region: zf-H2C2_5;
                     pfam13909"
                     /db_xref="CDD:206080"
     misc_feature    1405..1428
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H2P0.1);
                     Region: Neuroprotective peptide (NAP)"
     misc_feature    1570..1572
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    1582..1584
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    1582..1584
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="phosphorylation site"
     misc_feature    2470..2472
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    2551..2553
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    2647..2784
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="Homeodomain;  DNA binding domains involved in the
                     transcriptional regulation of key eukaryotic developmental
                     processes; may bind to DNA as monomers or as homo- and/or
                     heterodimers, in a sequence-specific manner; Region:
                     homeodomain; cd00086"
                     /db_xref="CDD:28970"
     misc_feature    order(2683..2685,2701..2703,2740..2742,2746..2751,
                     2758..2763,2767..2775,2779..2784)
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:28970"
     misc_feature    order(2749..2751,2758..2763,2770..2772)
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:28970"
     misc_feature    2758..2760
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    2971..2973
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    2977..2979
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    3106..3108
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    3202..3204
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    3202..3204
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="phosphorylation site"
     misc_feature    3208..3210
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot
                     (Q9H2P0.1); phosphorylation site"
     misc_feature    3208..3210
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="phosphorylation site"
     misc_feature    3448..3450
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine; propagated from
                     UniProtKB/Swiss-Prot (Q9H2P0.1); acetylation site"
     misc_feature    3469..3471
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine; propagated from
                     UniProtKB/Swiss-Prot (Q9H2P0.1); acetylation site"
     exon            454..546
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /inference="alignment:Splign:1.39.8"
     exon            547..4713
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /inference="alignment:Splign:1.39.8"
     STS             2421..2513
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="SHGC-57240"
                     /db_xref="UniSTS:43343"
     STS             2544..2653
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="D20S689"
                     /db_xref="UniSTS:153552"
     variation       2913
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /replace="c"
                     /replace="t"
                     /db_xref="dbSNP:1062651"
     variation       3011
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /replace="c"
                     /replace="g"
                     /db_xref="dbSNP:34342624"
     STS             3229..3380
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="D1S1984E"
                     /db_xref="UniSTS:151309"
     variation       4252
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /replace="c"
                     /replace="g"
                     /db_xref="dbSNP:11545910"
     STS             4455..4594
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="RH11804"
                     /db_xref="UniSTS:29802"
     STS             4529..4638
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="D20S772"
                     /db_xref="UniSTS:8216"
     STS             4542..4681
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="D20S953"
                     /db_xref="UniSTS:50575"
     STS             4580..4685
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /standard_name="SHGC-36586"
                     /db_xref="UniSTS:12497"
     polyA_signal    4687..4692
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
     polyA_site      4705
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
                     /experiment="experimental evidence, no additional details
                     recorded"
     polyA_site      4713
                     /gene="ADNP"
                     /gene_synonym="ADNP1"
ORIGIN

cggccgcggcgcgagccggagtccgccgagccggagcgcgacgaggccccgggcgcgccctccccgctgccgccaccgccgtgccgccgccatccgcccgccgccgccgccgctgtccggcccccgagcacgccggccccgcgcgcgcctcgaggccgagtcaaggtgtgagatgcacaatgcgaaacctaggccccagcttttacaccatgatgcgcagggttgtactttttgtactgaactgataggtggcctagtggttatgccctgtactaccattttgaggatctggactccgtttcctgccttgctctttggaccacattgtcaattcacaccgaaactatgttccaacttcctgtcaacaatcttggcagtttaagaaaagcccggaaaactgtgaaaaaaatacttagtgacattgggttggaatactgtaaagaacatatagaagattttaaacaatttgaacctaatgacttttatttgaaaaacactacatgggaggatgtaggactgtgggacccatcacttacgaaaaaccaggactatcggacaaaacctttctgctgcagcgcttgtccattttcctcaaaattcttctctgcctacaaaagtcatttccgcaatgtccatagtgaagactttgaaaataggattctccttaattgcccctactgtaccttcaatgcagacaaaaagactttggaaacacacattaaaatatttcatgctccgaacgccagcgcaccaagtagcagcctcagcactttcaaagataaaaacaaaaatgatggccttaaacctaagcaggctgacagtgtagagcaagctgtttattactgtaagaagtgcacttaccgagatcctctttatgaaatagttaggaagcacatttacagggaacattttcagcatgtggcagcaccttacatagcaaaggcaggagaaaaatcactcaatggggcagtccccttaggctcgaatgcccgagaagagagtagtattcactgcaagcgatgccttttcatgccaaagtcctatgaagctttggtacagcatgtcatcgaagaccatgaacgtataggctatcaggtcactgccatgattgggcacacaaatgtagtggttccccgatccaaacccttgatgctaattgctcccaaacctcaagacaagaagagcatgggactcccaccaaggatcggttcccttgcttctggaaatgtccggtctttaccatcacagcagatggtgaatcgactctcaataccaaagcctaacttaaattctacaggagtcaacatgatgtccagtgttcatctgcagcagaacaactatggagtcaaatctgtaggccagggttacagtgttggtcagtcaatgagactgggtctaggtggcaacgcaccagtttccattcctcaacaatctcagtctgtaaagcagttacttccaagtggaaacggaaggtcttatgggcttgggtcagagcagaggtcccaggcaccagcaagatactccctgcagtctgctaatgcctcttctctctcatcgggccagttaaagtctccttccctctctcagtcacaggcatccagagtgttaggtcagtccagttccaaacctgctgcagctgccacaggccctcccccaggtaacacttcctcaactcaaaagtggaaaatatgtacaatctgtaatgagctttttcctgaaaatgtctatagtgtgcacttcgaaaaagaacataaagctgagaaagtcccagcagtagccaactacattatgaaaatacacaattttactagcaaatgcctctactgtaatcgctatttacccacagatactctgctcaaccatatgttaattcatggtctgtcttgtccatattgccgttcaactttcaatgatgtggaaaagatggccgcacacatgcggatggttcacattgatgaagagatgggacctaaaacagattctactttgagttttgatttgacattgcagcagggtagtcacactaacatccatctcctggtaactacatacaatctgagggatgccccagctgaatctgttgcttaccatgcccaaaataatcctccagttcctccaaagccacagccaaaggttcaggaaaaggcagatatccctgtaaaaagttcacctcaagctgcagtgccctataaaaaagatgttgggaaaaccctttgtcctctttgcttttcaatcctaaaaggacccatatctgatgcacttgcacatcacttacgagagaggcaccaagttattcagacggttcatccagttgagaaaaagctcacctacaaatgtatccattgccttggtgtgtataccagcaacatgaccgcctcaactatcactctgcatctagttcactgcaggggcgttggaaagacccaaaatggccaggataagacaaatgcaccctctcggcttaatcagtctccaagtctggcacctgtgaagcgcacttacgagcaaatggaatttcccttactgaaaaaacgaaagttagatgatgatagtgattcacccagcttctttgaagagaagcctgaagagcctgttgttttagctttagaccccaagggtcatgaagatgattcctatgaagccaggaaaagctttctaacaaagtatttcaacaaacagccctatcccaccaggagagaaattgagaagctagcagccagtttatggttatggaagagtgacatcgcttcccattttagtaacaaaaggaagaagtgtgtccgtgattgtgaaaagtacaagcctggcgtgttgctggggtttaacatgaaagaattaaataaagtcaagcatgagatggattttgatgctgagtggctatttgaaaatcatgatgagaaggattccagagtcaatgctagtaagactgctgacaaaaagctcaaccttgggaaggaagatgacagttcctcagacagttttgaaaatttggaagaagaatccaatgaaagtggtagcccttttgaccctgtttttgaagttgaacctaaaatctctaacgataacccagaggaacatgtactgaaggtaattcctgaggatgcttcagaatctgaggagaagctagaccaaaaagaggatggttcaaaatacgaaactattcatttgactgaggaaccaaccaaactaatgcacaatgcatctgatagtgaggttgaccaagacgatgttgttgagtggaaagacggtgcttctccatctgagagtgggcctggatcccaacaagtgtcagactttgaggacaatacctgcgaaatgaaaccaggaacctggtctgacgagtcttcccaaagcgaagatgcaaggagcagtaagccagctgccaaaaaaaaggctaccatgcaaggtgacagagagcagttgaaatggaagaatagttcctatggaaaagttgaagggttttggtctaaggaccagtcacagtggaagaatgcatctgagaatgatgagcgcttatctaacccccagattgagtggcagaatagcacaattgacagtgaggatggggaacagtttgacaacatgactgatggagtagctgagcccatgcatggcagcttagccggagttaaactgagcagccaacaggcctaagtgccaggttccctggcgttggtgacatgctgcagcctggaactctgatctccagtgtgactgcaaagctgtcttctcactggtactgccttgtgagtactggttggactgtggggcatgtggccgctgcagttccagtggttatttctaagtctatgacaggacaggctgttcttgcttcagaaccttctctgacagacacggtaactaaatgtgaaaaaccaataagctggtgactcatgaatacacacgaggaaaagcagaggtttattttatctgccttttcaacatttctttccctctgtgaaatgattggtcagatgtctttgagaagtgttaaactaattcacatggtagtgtagggccaacatacaagctaccagtctaatgtgtatagtagactttgggaaaagcgattttttttcatgtattcattctgaatagttgaaatgtatatttgtacagtcttttagacctattcaagtgatgctcatgatcctgttactgtgtgcccatcatagatttctttttttagtgttgcccttgctgtgtaataaacgctctatctagtttacctagcaaaagctcaaaactgcgctagtatggactttttggacagacttagtttttgcacataaccttgtacaatcttgcaacagaggccagccacgtaagatatatatctggactctcttgtattataggatttttcttgttctgaatatccttgacattacagctgtcaaaaacaaaaactggtatttcagatctgttttctgaaatcttttaagctaaaatcacatgcaagaattgactttgcagctactaattttgacaccttttagatctgtataaaagtgtgttgtgttgaagcagcaaaccaatgagtgctgcattttggatatttagttttatctttagttcaacaccatcatggtggattcatttataccatctaatatatgacacactgttgtagtatgtataattttgtgatctttattttccctttgtattcattttaagcatctaaataaattgctgtattgtgcttaatgt

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