GGRNA | NM_001146185.1

LOCUS       NM_001146185            8375 bp    mRNA    linear   PRI 07-JUL-2013
DEFINITION  Homo sapiens paternally expressed 3 (PEG3), transcript variant 3,
            mRNA.
ACCESSION   NM_001146185
VERSION     NM_001146185.1  GI:226053147
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Haggarty,P., Hoad,G., Campbell,D.M., Horgan,G.W., Piyathilake,C.
            and McNeill,G.
  TITLE     Folate in pregnancy and imprinted gene and repeat element
            methylation in the offspring
  JOURNAL   Am. J. Clin. Nutr. 97 (1), 94-99 (2013)
   PUBMED   23151531
  REMARK    GeneRIF: None of the folate cycle genotypes in the mother or infant
            were related to the methylation of IGF2, PEG3, or LINE-1.
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Jiang,X., Yu,Y., Yang,H.W., Agar,N.Y., Frado,L. and Johnson,M.D.
  TITLE     The imprinted gene PEG3 inhibits Wnt signaling and regulates glioma
            growth
  JOURNAL   J. Biol. Chem. 285 (11), 8472-8480 (2010)
   PUBMED   20064927
  REMARK    GeneRIF: mammalian imprinting utilizes Peg3/Pw1 to co-opt the Wnt
            pathway, thereby regulating development and glioma growth
REFERENCE   3  (bases 1 to 8375)
  AUTHORS   Wheeler,H.E., Metter,E.J., Tanaka,T., Absher,D., Higgins,J.,
            Zahn,J.M., Wilhelmy,J., Davis,R.W., Singleton,A., Myers,R.M.,
            Ferrucci,L. and Kim,S.K.
  TITLE     Sequential use of transcriptional profiling, expression
            quantitative trait mapping, and gene association implicates MMP20
            in human kidney aging
  JOURNAL   PLoS Genet. 5 (10), E1000685 (2009)
   PUBMED   19834535
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   4  (bases 1 to 8375)
  AUTHORS   Huang,J.M. and Kim,J.
  TITLE     DNA methylation analysis of the mammalian PEG3 imprinted domain
  JOURNAL   Gene 442 (1-2), 18-25 (2009)
   PUBMED   19397955
  REMARK    GeneRIF: PEG3 imprinted domain of humans, cows, and mice contains
            differing numbers of differentially methylated regions (DMR), but
            the PEG3-CpG island is the only DMR that is conserved among these
            three species.
REFERENCE   5  (bases 1 to 8375)
  AUTHORS   Otsuka,S., Maegawa,S., Takamura,A., Kamitani,H., Watanabe,T.,
            Oshimura,M. and Nanba,E.
  TITLE     Aberrant promoter methylation and expression of the imprinted PEG3
            gene in glioma
  JOURNAL   Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. 85 (4), 157-165 (2009)
   PUBMED   19367087
  REMARK    GeneRIF: abnormal regulation of PEG3 is associated with several
            glioma subtypes and that it plays an important role in
            tumorigenesis.
REFERENCE   6  (bases 1 to 8375)
  AUTHORS   Hiby,S.E., Lough,M., Keverne,E.B., Surani,M.A., Loke,Y.W. and
            King,A.
  TITLE     Paternal monoallelic expression of PEG3 in the human placenta
  JOURNAL   Hum. Mol. Genet. 10 (10), 1093-1100 (2001)
   PUBMED   11331620
  REMARK    GeneRIF: PEG3 gene is imprinted, with preferential expression from
            the paternal allele in human placenta.
REFERENCE   7  (bases 1 to 8375)
  AUTHORS   Relaix,F., Wei,Xj., Li,W., Pan,J., Lin,Y., Bowtell,D.D.,
            Sassoon,D.A. and Wu,X.
  TITLE     Pw1/Peg3 is a potential cell death mediator and cooperates with
            Siah1a in p53-mediated apoptosis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (5), 2105-2110 (2000)
   PUBMED   10681424
REFERENCE   8  (bases 1 to 8375)
  AUTHORS   Kim,J., Bergmann,A. and Stubbs,L.
  TITLE     Exon sharing of a novel human zinc-finger gene, ZIM2, and
            paternally expressed gene 3 (PEG3)
  JOURNAL   Genomics 64 (1), 114-118 (2000)
   PUBMED   10708526
REFERENCE   9  (bases 1 to 8375)
  AUTHORS   Relaix,F., Wei,X.J., Wu,X. and Sassoon,D.A.
  TITLE     Peg3/Pw1 is an imprinted gene involved in the TNF-NFkappaB signal
            transduction pathway
  JOURNAL   Nat. Genet. 18 (3), 287-291 (1998)
   PUBMED   9500555
REFERENCE   10 (bases 1 to 8375)
  AUTHORS   Kim,J., Ashworth,L., Branscomb,E. and Stubbs,L.
  TITLE     The human homolog of a mouse-imprinted gene, Peg3, maps to a zinc
            finger gene-rich region of human chromosome 19q13.4
  JOURNAL   Genome Res. 7 (5), 532-540 (1997)
   PUBMED   9149948
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK295679.1, AF208969.1,
            AF208967.1, BC037330.2, CR933682.1 and AI089069.1.
            
            Summary: In human, ZIM2 and PEG3 are treated as two distinct genes
            though they share multiple 5' exons and a common promoter and both
            genes are paternally expressed (PMID:15203203). Alternative
            splicing events connect their shared 5' exons either with the
            remaining 4 exons unique to ZIM2, or with the remaining 2 exons
            unique to PEG3. In contrast, in other mammals ZIM2 does not undergo
            imprinting and, in mouse, cow, and likely other mammals as well,
            the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein
            belongs to the Kruppel C2H2-type zinc finger protein family. PEG3
            may play a role in cell proliferation and p53-mediated apoptosis.
            PEG3 has also shown tumor suppressor activity and tumorigenesis in
            glioma and ovarian cells. Alternative splicing of this PEG3 gene
            results in multiple transcript variants encoding distinct isoforms.
            [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (3) is missing a 5' non-coding
            exon, and uses an alternate acceptor splice site at the first
            coding exon compared to variant 1. This results in the use of an
            in-frame downstream start codon, and an isoform (2) with a shorter
            N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF208969.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           ERS025084 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene :: PMID: 11331620
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK295679.1         1-30
            31-4768             AF208969.1         1-4738
            4769-6011           AF208967.1         5129-6371
            6012-7378           BC037330.2         1379-2745
            7379-8242           CR933682.1         4949-5812
            8243-8375           AI089069.1         1-133               c
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.4"
     gene            1..8375
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /note="paternally expressed 3"
                     /db_xref="GeneID:5178"
                     /db_xref="HGNC:8826"
                     /db_xref="MIM:601483"
     exon            1..145
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     variation       23
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /replace="g"
                     /replace="t"
                     /db_xref="dbSNP:28384422"
     exon            146..232
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    203..205
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /note="upstream in-frame stop codon"
     exon            233..398
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     CDS             383..4771
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /note="isoform 2 is encoded by transcript variant 3; zinc
                     finger and SCAN domain-containing protein 24; Kruppel-type
                     zinc finger protein; paternally-expressed gene 3 protein"
                     /codon_start=1
                     /product="paternally-expressed gene 3 protein isoform 2"
                     /protein_id="NP_001139657.1"
                     /db_xref="GI:226053148"
                     /db_xref="CCDS:CCDS58685.1"
                     /db_xref="GeneID:5178"
                     /db_xref="HGNC:8826"
                     /db_xref="MIM:601483"
                     /translation="

MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLSLPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLVQLAEDDGHSHMTQGHSSRSKRSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARESSDRSQRFPRMSDDNWKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSIHDQKGCPRKKPFECGSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMHTRENLYEYGESFIHSVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAFMPSPTFSELQKIYGKDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPALNEFQKMYGKEKMYECKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKLCDFTDGRDAFMQSSELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISSNPYENQKIPTKENVYEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNTSEGREYSRSVIHSLVASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDSVIQSVFRAKPQKSVPGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRGMLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDFRQFFATSEDLNTNQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDDKIYECEDCGLGFVDLTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIHSSFLFEHQRIHEQDQLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHMRLHREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYTHTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLRIQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGAGIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKTHASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG

"
     exon            399..485
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     exon            486..569
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     exon            570..673
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     exon            674..776
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     exon            777..866
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     exon            867..8369
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /inference="alignment:Splign:1.39.8"
     STS             1417..1661
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="PEG3"
                     /db_xref="UniSTS:504454"
     STS             4594..4693
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="D19S699E"
                     /db_xref="UniSTS:150599"
     variation       5474
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:1055359"
     STS             6029..6298
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="RH18153"
                     /db_xref="UniSTS:209605"
     STS             6170..6298
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="SHGC-32171"
                     /db_xref="UniSTS:78243"
     STS             7169..8296
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="WI-22317"
                     /db_xref="UniSTS:29071"
     STS             8096..8303
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="RH17474"
                     /db_xref="UniSTS:60385"
     STS             8195..8319
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
                     /standard_name="SGC34114"
                     /db_xref="UniSTS:19187"
     polyA_signal    8341..8346
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
     polyA_signal    8347..8352
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
     polyA_signal    8359..8364
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
     polyA_site      8369
                     /gene="PEG3"
                     /gene_synonym="PW1; ZKSCAN22; ZNF904; ZSCAN24"
ORIGIN

gcagaagtctgggcagctgcgggaggagaggtttgggaggcgcgggagatgtccaccctgggctggtggcgccgccgggcgccgggcgccatgagggtgcgctaggcggctgttcgtgcccgaggctgcgcagcactgaggtgagctttgccttcttgatcttccgtccttcttggagacgactggcgagaggaagagggactaggtccaaacgctaggtggctgggtccagccggagacccgcaccaaggaggagatcatcgagctcttggtccttgagcagtacctgaccatcatccctgaaaagctcaagccttgggtgcgagcaaaaaagccggagaactgtgagaagctcgtcactctgctggagaattacaaggagatgtaccaaccagaagacgacaacaacagtgacgtgaccagcgacgacgacatgacccggaacagaagagagtcctcaccacctcactcagtccattctttcagtgaccgggactgggaccggaggggcagaagcagagacatggagccacgagaccgctggtcccacaccaggaacccaagaagcaggatgcctccgcgggatctttcccttcctgtggtggcgaaaacaagctttgaaatggacagagaggacgacagggactccagggcttatgagtcccgatctcaggatgctgaatcataccaaaatgtggtggacctcgctgaggacaggaaacctcacaacacaatccaggacaacatggaaaactacaggaagctgctctccctcgtgcagcttgctgaagacgatggccactcccacatgacgcagggccactcatcaagatccaagagaagtgcctacccaagcaccagtcgaggtctaaaaactatgcctgaagccaaaaaatcaacccaccggcgggggatttgtgaagatgaatcttcccacggagtgataatggaaaaattcatcaaggatgtgtcacgcagttccaaatcgggaagagcaagggagtcaagcgaccggtcacagagattccccagaatgtcagatgataactggaaggacatttcattgaacaagagggagtcagtgatccagcagcgggtttatgaagggaatgcatttaggggaggctttaggtttaattcaacccttgtttccagaaagagagttcttgaaagaaagaggcgctatcattttgacacagatgggaagggctcgattcacgatcaaaaaggctgtcccaggaagaagccctttgaatgtggtagtgagatgagaaaagccatgagcgtgagcagcctgagcagcctcagctccccctcctttaccgagtcacagccaattgattttggggcaatgccatatgtatgtgatgagtgtgggaggtcgttcagtgtcatctcagaatttgttgagcaccagatcatgcatactagagagaacctctatgagtatggtgagtcctttatccacagtgtggctgtcagtgaagttcagaaaagtcaggttggagggaaacgttttgaatgtaaggactgtggagagaccttcaataagagtgccgccttggctgaacatcggaagattcatgctagaggttatcttgtggaatgtaagaatcaggaatgtgaggaagccttcatgcctagccccacctttagtgagcttcagaaaatatatggcaaagacaaattctacgagtgcagggtgtgtaaggaaaccttccttcatagttctgccctgattgagcaccagaaaatccactttggggatgacaaagataatgagcgtgaacatgaacgtgaacgtgaacgtgagcgcggggaaacctttaggcccagcccagcccttaatgagtttcagaaaatgtatggtaaagagaaaatgtacgaatgtaaggtgtgtggggagactttccttcatagctcatccctgaaagaacatcagaaaatccatactagagggaacccatttgaaaacaagggtaaagtgtgtgaggaaacctttattcctggtcagtcccttaaaaggcgtcagaaaacttacaataaggagaagctctgtgactttacagatggccgggatgccttcatgcaaagctcagagctcagtgagcatcagaaaattcattctcgaaagaacctctttgaaggcagagggtatgagaaatctgtcattcatagtgggccattcactgaatctcagaagagtcatactataacaagacctcttgaaagtgatgaggacgaaaaggcgttcaccattagctctaacccctatgaaaaccagaagattcccactaaggaaaatgtctatgaggcaaaatcatatgagaggtctgttattcatagcttagcctctgtggaagctcagaaaagtcacagtgtagcagggcccagtaaaccaaaagtaatggcagagtctaccattcagagcttcgatgctatcaaccatcagagagttcgtgctggagggaacacctctgaaggaagggaatacagtaggtctgttatccatagcttagtggcttccaaacctccaagaagtcacaatggaaatgaattggtggaatctaatgagaagggagaatcctccatttatatctcagaccttaatgataagcgacagaagattcctgccagagagaacccttgtgaagggggcagtaagaatcgcaactatgaagactctgtcatacagagtgtattccgtgccaaacctcagaaaagtgttcctggagagggatctggtgagtttaagaaggatggcgaattctctgttcccagctcaaatgtccgtgaataccagaaggctcgtgctaaaaagaaatacattgagcataggagcaatgagacctctgtaattcactctctgccttttggtgaacaaacatttcgccctcgagggatgctctatgaatgtcaggagtgtggggagtgctttgctcatagctctgacctcactgagcaccagaagattcatgatagggagaagccctctggaagcagaaactatgaatggtctgtcattcgcagcttggcccctactgaccctcaaacaagttacgcccaagagcagtatgctaaagagcaagcgcggaacaaatgtaaggacttcagacaattttttgctaccagcgaagacctcaacacaaaccagaaaatctatgaccaagagaagtctcatggcgaggagtctcaaggcgagaatactgatggggaggagacccacagcgaggagacccatggtcaggagacaattgaagaccctgtcattcaaggctcagacatggaagaccctcagaaggatgaccctgatgacaaaatctatgaatgtgaggactgtggcctgggctttgtggatctcacagacctcacagaccatcagaaagtccacagcaggaagtgcctggttgacagtcgggagtacacacattctgtaattcacacccattccatcagcgagtatcagagagattacactggagagcagctgtatgaatgtccaaagtgtggggaatcttttattcatagctcattccttttcgagcatcagagaatccatgaacaagaccagttgtattccatgaaggggtgtgatgatggttttattgccctcttgcccatgaagccacggaggaatcgtgctgcagagaggaatcctgctcttgctgggtcggccattcgatgccttttgtgtggacaaggcttcattcatagctctgcccttaatgagcatatgagacttcatagggaagatgatttactggagcagagccagatggctgaggaagctatcattccaggcttagccctcactgagtttcagagaagtcagaccgaagagagactctttgaatgtgcagtctgtggagaatctttcgtcaacccagcagaacttgcagatcacgtaactgttcataagaatgagccctatgagtacgggtcctcctatactcacacctcatttcttactgagcccctcaaaggagctataccattctatgaatgcaaggattgtggtaagtcctttattcatagcacagtcctcactaaacataaggagcttcatctggaagaagaagaagaagatgaagcagcagcagctgcagcagcagcagcccaggaagttgaagccaatgtccatgttccacaagtagttctgaggattcagggcttaaacgtagaggctgctgagccagaagtggaggctgccgagccagaagtggaggctgctgagccagaagtggaggctgctgagccaaacggagaggctgaagggccagatggagaggctgcagagcccattggagaggctggacagccaaatggagaggccgagcagccaaatggggatgctgatgagccagatggtgcaggtattgaagacccagaagaaagagctgaagagccagagggaaaagctgaagagccagagggagatgccgacgagcctgacggtgtgggaattgaagacccagaagaaggtgaagatcaagagattcaggtagaagaaccatactatgactgccatgaatgcacagaaaccttcacttccagcacagcattcagtgaacacctgaaaactcatgccagcatgatcatatttgagcctgcaaatgcctttggggagtgctcaggctacatcgaacgtgccagcaccagcacaggtggtgccaatcaagctgatgagaagtacttcaaatgtgacgtctgtgggcagctcttcaatgaccgcctgtccctcgccagacaccagaatacccacactggctgagggcatggggtaaaggttagaaaaccttcacctaggacttgacccttaccaaaccacagagaatccaaaccaatccatgataatgtcagtaggagacttaaccttagtgtgttacacacctgacttaacatctctaaactcagattgaaaagagaccgaatgtgcagattccacagtcttaagctttccccttcagatgtcagtgtctgcatgtgggaaagccatagcacacatcttacctttccaagtaatcagattgagaaaaccctatgagtattccagactacagagtttgcccaaatcaactgtaaatgacacttgtgtaacgtatatatagtgtttcatgaggtgtatataaaatagcaaattatgacagaacagtgatcacatatatttggatttatatgatatacagttacagtttactctgcagaggtaccttacctggtattctttgaattttttttttttttggaggaggaagagagcaacaaatttgattatatttttaagtgtcttagatcctgagaaagatttattgtgcattatttgaaccctgtcaatatctttttgagtaattgttttgtttcttacccttaaatagtcttgtgaagctgtaggcatgatagataacatggcttttactccttactgtttgaaaagataagtactttagcttctttctgcagccatttcatctgcgccaacactttggaacctaatactgtgtaaggctttacaatatacggattggctttttgtgacccagattgattggttgccacatgttatgtttgttgaagtggttctcatgcaaaaatattacacatttgtgttctgggttttttttttttttaaccaactcaatatgtgtttgatgatagtgaattgataaaacccgaagcttttccctgtaaatcttacatctttgcctttaaagaatgggttacaaccatcactagatcacagtagtgcctaatgaaggttgagaaccgtaggagaggctctcatgctgtaaataatgttgcaggctaataacctttcatcacttcctttgtgcgcttcctgccttaagtgacaagtagcaacatggcttgggtcccctgtgcagcatcagcttatgctgccacaagtcagtttgcaccctaggtgcccaggagctagtatccttagatctttctatcgctaacttaattctcttcgttatttatctgaccctctaactccatgtctaacttgcattaaaaaaaaaaaaattctttacagtcaacccaagcttaacatggactcaggttccccagcagccttaatttgttttgttaacatctgttccttctttttcagctctcctagagtatttctgagtgttgtgttcatctaatcttagtattcttttaattacaaattgacctcacagcttgaggtttcttgtgtcctattctgtggactacctgtgctcctttgcttcccctcccctcgcataataactatattaagaaattttttttggccttgagttggctggaaaaaaaatataaaatttaaaaaatttaaaaaaaaagatttgcaaaatgtaagtgtagatcatttgaacaagcaaaattaaagtacccactgggggaaatgtgtctgaatcttactcttctggatctgcaggattagggcttggaagtatgtcaaagatgcagggagtgtcaaagtttaggaagattgtagagctgagagcaagaagcagaaatgagtgagtcaaagaagggagtcctaatatatcaccagatctaggaggggagaggagacagacagaagaaaacaccagaggcaagaactgtagaaggccaggtttctgagaatgaattgagcggggtgtcctgagcagtttggaaaaggagtttttgatggtatggtgtaggtgagggctggctgcataggaaggactgaggttggaacggacatcgggaaagctgaggggcagtgaggtttactacatgggaaaaggactcttgaaacgagaatcagtgttgatgtcggggtgaactttgtgggtacattacttggtgttaacattgttggcagtggtagccccttttcagaaagcaacttgctgtaagtcagggtgtccgttccaaccttcagctagtgaaaaggtagtaacaaatggtaaacaagagaatgattgtttaaacctatctgtggacacttaatgcaactgtttaaaaatgataatcacgagttatgtagcaacgtggaaatatatttacagaacattaagtggagaaagcaggacacgaaagtatatttatactacagttataactcaacagttcatttatatgctgttcatttaacagttcatttaaacagttcattataactgtttaaaaatatatatgcttatagtcaaaagctgttgtggtgttgttgttgtaggcttatagttgagcattattttcttaaatttcttgaatgttctttatggtagtgttactaaaaagtttatgatcacattttcattgtgaacataatttgaactcattatcacacacttggaaaatacagaaaagtggaggaaaaaaaatcatatccccaccatccaaagacatatactctcctcttatcttgttcattcttgtttctgtgcacaggtttatgattataactgtgtcaaaatgtatattcaaaatagctgttacattacctttgtggaattatggttaaatactttcactttaattttttcaaatgttccctataataatgtcctgataacagtgtattatgtgtgtctccattggtgtgcataatacatacccagaggaaaaattagaaaataaagtaaattattttaaaaaattacctatattcccaacacctaacaactactgctaacatcttgatctgtttcctctatcttgtttcagtgcacacgcttgtgataacagtgttaaatatgtgtgcataaagtcttaaatgaaaagatgtggaaaataactaaaatagtgttgtcattgtgggaatttggttaaatattttgtctcaaattccttaaataatctttggtgttttggtaataaattttatgtatgtattttccattacaaatataatacatactcatacaaaactttggaaattcagtaaagaaaattcacacatattcccaacacccaacaacaattaactgttaacatcttgatctgtgcactagtctgtgattattagggtgttagtgataagtatgcataaatgtcaaagatgggaagaaagatgaaaaacaagaaatagttgtgtggttgttgtgggattatggttattttgtttcggtttccttgaaaggtcatcattctagtgttttggtagtccacctttactacatatatttccattatatatgaaatgtgttcattatagaaactttgaagttacagaaatgtagaagagaaactcacccatgttttcaccatccaaagagtgtggttaacatcttgatatattttcttcatcttgtttctgtgcacaggtttttggtttgttaatatggttgtggtcattctatctgtaatagtgtcaacaataaaaataaagttaaaaataaatatttaaaaaa

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