GGRNA | NM_001144769.2

LOCUS       NM_001144769           18103 bp    mRNA    linear   PRI 29-APR-2013
DEFINITION  Homo sapiens dystonin (DST), transcript variant 2, mRNA.
ACCESSION   NM_001144769
VERSION     NM_001144769.2  GI:469832270
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 18103)
  AUTHORS   Pasdeloup,D., McElwee,M., Beilstein,F., Labetoulle,M. and
            Rixon,F.J.
  TITLE     Herpesvirus tegument protein pUL37 interacts with dystonin/BPAG1 to
            promote capsid transport on microtubules during egress
  JOURNAL   J. Virol. 87 (5), 2857-2867 (2013)
   PUBMED   23269794
  REMARK    GeneRIF: study identifies dystonin, a cytoskeleton cross-linker
            involved in microtubule-based transport, as a binding partner of
            the HSV-1 protein pUL37, implicated in capsid transport; study
            provides insight into cellular requirements for HSV-1 capsid
            transport and identifies dystonin as a nonmotor protein part of
            transport machinery
REFERENCE   2  (bases 1 to 18103)
  AUTHORS   Edvardson,S., Cinnamon,Y., Jalas,C., Shaag,A., Maayan,C.,
            Axelrod,F.B. and Elpeleg,O.
  TITLE     Hereditary sensory autonomic neuropathy caused by a mutation in
            dystonin
  JOURNAL   Ann. Neurol. 71 (4), 569-572 (2012)
   PUBMED   22522446
  REMARK    GeneRIF: This is the first report of a defect in the neuronal
            isoform of dystonin in humans.
REFERENCE   3  (bases 1 to 18103)
  AUTHORS   Hamill,K.J., Hopkinson,S.B., Jonkman,M.F. and Jones,J.C.
  TITLE     Type XVII collagen regulates lamellipod stability, cell motility,
            and signaling to Rac1 by targeting bullous pemphigoid antigen 1e to
            alpha6beta4 integrin
  JOURNAL   J. Biol. Chem. 286 (30), 26768-26780 (2011)
   PUBMED   21642434
  REMARK    GeneRIF: in motile cells Col XVII recruits BPAG1e to alpha6beta4
            integrin and is necessary for activation of signaling pathways,
            motile behavior, and lamellipodial stability.
REFERENCE   4  (bases 1 to 18103)
  AUTHORS   Steiner-Champliaud,M.F., Schneider,Y., Favre,B., Paulhe,F.,
            Praetzel-Wunder,S., Faulkner,G., Konieczny,P., Raith,M., Wiche,G.,
            Adebola,A., Liem,R.K., Langbein,L., Sonnenberg,A., Fontao,L. and
            Borradori,L.
  TITLE     BPAG1 isoform-b: complex distribution pattern in striated and heart
            muscle and association with plectin and alpha-actinin
  JOURNAL   Exp. Cell Res. 316 (3), 297-313 (2010)
   PUBMED   19932097
  REMARK    GeneRIF: BPAG1-b was detectable in vitro and in vivo as a high
            molecular mass protein in striated and heart muscle cells,
            co-localizing with alpha-actinin-2 and partially with the
            cytolinker plectin as well as with the intermediate filament
            protein desmin.
REFERENCE   5  (bases 1 to 18103)
  AUTHORS   Okumura,M., Yamakawa,H., Ohara,O. and Owaribe,K.
  TITLE     Novel alternative splicings of BPAG1 (bullous pemphigoid antigen 1)
            including the domain structure closely related to MACF (microtubule
            actin cross-linking factor)
  JOURNAL   J. Biol. Chem. 277 (8), 6682-6687 (2002)
   PUBMED   11751855
REFERENCE   6  (bases 1 to 18103)
  AUTHORS   Brown,A., Dalpe,G., Mathieu,M. and Kothary,R.
  TITLE     Cloning and characterization of the neural isoforms of human
            dystonin
  JOURNAL   Genomics 29 (3), 777-780 (1995)
   PUBMED   8575775
REFERENCE   7  (bases 1 to 18103)
  AUTHORS   Sawamura,D., Li,K., Chu,M.L. and Uitto,J.
  TITLE     Human bullous pemphigoid antigen (BPAG1). Amino acid sequences
            deduced from cloned cDNAs predict biologically important peptide
            segments and protein domains
  JOURNAL   J. Biol. Chem. 266 (27), 17784-17790 (1991)
   PUBMED   1717441
REFERENCE   8  (bases 1 to 18103)
  AUTHORS   Tanaka,T., Parry,D.A., Klaus-Kovtun,V., Steinert,P.M. and
            Stanley,J.R.
  TITLE     Comparison of molecularly cloned bullous pemphigoid antigen to
            desmoplakin I confirms that they define a new family of cell
            adhesion junction plaque proteins
  JOURNAL   J. Biol. Chem. 266 (19), 12555-12559 (1991)
   PUBMED   1712022
REFERENCE   9  (bases 1 to 18103)
  AUTHORS   Sawamura,D., Li,K.H., Nomura,K., Sugita,Y., Christiano,A.M. and
            Uitto,J.
  TITLE     Bullous pemphigoid antigen: cDNA cloning, cellular expression, and
            evidence for polymorphism of the human gene
  JOURNAL   J. Invest. Dermatol. 96 (6), 908-915 (1991)
   PUBMED   2045679
REFERENCE   10 (bases 1 to 18103)
  AUTHORS   Sawamura,D., Nomura,K., Sugita,Y., Mattei,M.G., Chu,M.L.,
            Knowlton,R. and Uitto,J.
  TITLE     Bullous pemphigoid antigen (BPAG1): cDNA cloning and mapping of the
            gene to the short arm of human chromosome 6
  JOURNAL   Genomics 8 (4), 722-726 (1990)
   PUBMED   2276744
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN994324.1, AK299898.1,
            AK295864.1, BC065536.1, AF400226.1, AL512448.9, AK055189.1,
            AB018271.2 and CD367397.1.
            On Mar 23, 2013 this sequence version replaced gi:221316617.
            
            Summary: This gene encodes a member of the plakin protein family of
            adhesion junction plaque proteins. Multiple alternatively spliced
            transcript variants encoding distinct isoforms have been found for
            this gene, but the full-length nature of some variants has not been
            defined. It has been reported that some isoforms are expressed in
            neural and muscle tissue, anchoring neural intermediate filaments
            to the actin cytoskeleton, and some isoforms are expressed in
            epithelial tissue, anchoring keratin-containing intermediate
            filaments to hemidesmosomes. Consistent with the expression, mice
            defective for this gene show skin blistering and neurodegeneration.
            [provided by RefSeq, Mar 2010].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments. This RefSeq
            is supported by human DN994324.1 and U31851.1, and mouse U25158.1
            and DQ023311.2. It is also discussed in PubMed ID: 8575775 and
            related publications.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-564               DN994324.1         9-572
            565-1501            AK299898.1         236-1172
            1502-1720           AK295864.1         1429-1647
            1721-4662           BC065536.1         240-3181
            4663-9405           AF400226.1         3110-7852
            9406-9992           AL512448.9         54594-55180         c
            9993-12399          AK055189.1         1278-3684
            12400-17835         AB018271.2         245-5680
            17836-18103         CD367397.1         1-268               c
FEATURES             Location/Qualifiers
     source          1..18103
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.1"
     gene            1..18103
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="dystonin"
                     /db_xref="GeneID:667"
                     /db_xref="HGNC:1090"
                     /db_xref="MIM:113810"
     CDS             42..17069
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     bullous pemphigoid antigen 1, 230/240kDa; hemidesmosomal
                     plaque protein; trabeculin-beta; dystonia musculorum
                     protein"
                     /codon_start=1
                     /product="bullous pemphigoid antigen 1 isoform 2"
                     /protein_id="NP_001138241.1"
                     /db_xref="GI:221316618"
                     /db_xref="GeneID:667"
                     /db_xref="HGNC:1090"
                     /db_xref="MIM:113810"
                     /translation="

MIAAAFLVLLRPYSIQCALFLLLLLLGTIATIVFFCCWHRKLQKGRHPMKSVFSGRSRSRDAVLRSHHFRSEGFRASPRHLRRRVAAAAAARLEEVKPVVEVHHQSEQETSVRKRRIKKSSRVQPEFYHSVQGASIRRPSSGNASYRCSMSSSADFSDEDDFSQKSGSASPAPGDTLPWNLPKHERSKRKIQGGSVLDPAERAVLRIADERDKVQKKTFTKWINQHLMKVRKHVNDLYEDLRDGHNLISLLEVLSGDTLPREKGRMRFHRLQNVQIALDYLKRRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIHVTGESEDMSAKERLLLWTQQATEGYAGIRCENFTTCWRDGKLFNAIIHKYRPDLIDMNTVAVQSNLANLEHAFYVAEKIGVIRLLDPEDVDVSSPDEKSVITYVSSLYDAFPKVPEGGEGIGANDVEVKWIEYQNMVNYLIQWIRHHVTTMSERTFPNNPVELKALYNQYLQFKETEIPPKETEKSKIKRLYKLLEIWIEFGRIKLLQGYHPNDIEKEWGKLIIAMLEREKALRPEVERLEMLQQIANRVQRDSVICEDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSGLTQSLTPSLTSSSMTSGLSSGMTSRLTPSVTPAYTPGFPSGLVPNFSSGVEPNSLQTLKLMQIRKPLLKSSLLDQNLTEEEINMKFVQDLLNWVDEMQVQLDRTEWGSDLPSVESHLENHKNVHRAIEEFESSLKEAKISEIQMTAPLKLTYAEKLHRLESQYAKLLNTSRNQERHLDTLHNFVSRATNELIWLNEKEEEEVAYDWSERNTNIARKKDYHAELMRELDQKEENIKSVQEIAEQLLLENHPARLTIEAYRAAMQTQWSWILQLCQCVEQHIKENTAYFEFFNDAKEATDYLRNLKDAIQRKYSCDRSSSIHKLEDLVQESMEEKEELLQYKSTIANLMGKAKTIIQLKPRNSDCPLKTSIPIKAICDYRQIEITIYKDDECVLANNSHRAKWKVISPTGNEAMVPSVCFTVPPPNKEAVDLANRIEQQYQNVLTLWHESHINMKSVVSWHYLINEIDRIRASNVASIKTMLPGEHQQVLSNLQSRFEDFLEDSQESQVFSGSDITQLEKEVNVCKQYYQELLKSAEREEQEESVYNLYISEVRNIRLRLENCEDRLIRQIRTPLERDDLHESVFRITEQEKLKKELERLKDDLGTITNKCEEFFSQAAASSSVPTLRSELNVVLQNMNQVYSMSSTYIDKLKTVNLVLKNTQAAEALVKLYETKLCEEEAVIADKNNIENLISTLKQWRSEVDEKRQVFHALEDELQKAKAISDEMFKTYKERDLDFDWHKEKADQLVERWQNVHVQIDNRLRDLEGIGKSLKYYRDTYHPLDDWIQQVETTQRKIQENQPENSKTLATQLNQQKMLVSEIEMKQSKMDECQKYAEQYSATVKDYELQTMTYRAMVDSQQKSPVKRRRMQSSADLIIQEFMDLRTRYTALVTLMTQYIKFAGDSLKRLEEEEKSLEEEKKEHVEKAKELQKWVSNISKTLKDAEKAGKPPFSKQKISSEEISTKKEQLSEALQTIQLFLAKHGDKMTDEERNELEKQVKTLQESYNLLFSESLKQLQESQTSGDVKVEEKIVAERQQEYKEKLQGICDLLTQTENRLIGHQEAFMIGDGTVELKKYQSKQEELQKDMQGSAQALAEVVKNTENFLKENGEKLSQEDKALIEQKLNEAKIKCEQLNLKAEQSKKELDKVVTTAIKEETEKVAAVKQLEESKTKIENLLDWLSNVDKDSERAGTKHKQVIEQNGTHFQEGDGKSAIGEEDEVNGNLLETDVDGQVGTTQENLNQQYQKVKAQHEKIISQHQAVIIATQSAQVLLEKQGQYLSPEEKEKLQKNMKELKVHYETALAESEKKMKLTHSLQEELEKFDADYTEFEHWLQQSEQELENLEAGADDINGLMTKLKRQKSFSEDVISHKGDLRYITISGNRVLEAAKSCSKRDGGKVDTSATHREVQRKLDHATDRFRSLYSKCNVLGNNLKDLVDKYQHYEDASCGLLAGLQACEATASKHLSEPIAVDPKNLQRQLEETKALQGQISSQQVAVEKLKKTAEVLLDARGSLLPAKNDIQKTLDDIVGRYEDLSKSVNERNEKLQITLTRSLSVQDGLDEMLDWMGNVESSLKEQGQVPLNSTALQDIISKNIMLEQDIAGRQSSINAMNEKVKKFMETTDPSTASSLQAKMKDLSARFSEASHKHKETLAKMEELKTKVELFENLSEKLQTFLETKTQALTEVDVPGKDVTELSQYMQESTSEFLEHKKHLEVLHSLLKEISSHGLPSDKALVLEKTNNLSKKFKEMEDTIKEKKEAVTSCQEQLDAFQVLVKSLKSWIKETTKKVPIVQPSFGAEDLGKSLEDTKKLQEKWSLKTPEIQKVNNSGISLCNLISAVTTPAKAIAAVKSGGAVLNGEGTATNTEEFWANKGLTSIKKDMTDISHGYEDLGLLLKDKIAELNTKLSKLQKAQEESSAMMQWLQKMNKTATKWQQTPAPTDTEAVKTQVEQNKSFEAELKQNVNKVQELKDKLTELLEENPDTPEAPRWKQMLTEIDSKWQELNQLTIDRQQKLEESSNNLTQFQTVEAQLKQWLVEKELMVSVLGPLSIDPNMLNTQRQQVQILLQEFATRKPQYEQLTAAGQGILSRPGEDPSLRGIVKEQLAAVTQKWDSLTGQLSDRCDWIDQAIVKSTQYQSLLRSLSDKLSDLDNKLSSSLAVSTHPDAMNQQLETAQKMKQEIQQEKKQIKVAQALCEDLSALVKEEYLKAELSRQLEGILKSFKDVEQKAENHVQHLQSACASSHQFQQMSRDFQAWLDTKKEEQNKSHPISAKLDVLESLIKDHKDFSKTLTAQSHMYEKTIAEGENLLLKTQGSEKAALQLQLNTIKTNWDTFNKQVKERENKLKESLEKALKYKEQVETLWPWIDKCQNNLEEIKFCLDPAEGENSIAKLKSLQKEMDQHFGMVELLNNTANSLLSVCEIDKEVVTDENKSLIQKVDMVTEQLHSKKFCLENMTQKFKEFQEVSKESKRQLQCAKEQLDIHDSLGSQAYSNKYLTMLQTQQKSLQALKHQVDLAKRLAQDLVVEASDSKGTSDVLLQVETIAQEHSTLSQQVDEKCSFLETKLQGIGHFQNTIREMFSQFAEFDDELDSMAPVGRDAETLQKQKETIKAFLKKLEALMASNDNANKTCKMMLATEETSPDLVGIKRDLEALSKQCNKLLDRAQAREEQVEGTIKRLEEFYSKLKEFSILLQKAEEHEESQGPVGMETETINQQLNMFKVFQKEEIEPLQGKQQDVNWLGQGLIQSAAKSTSTQGLEHDLDDVNARWKTLNKKVAQRAAQLQEALLHCGRFQDALESLLSWMVDTEELVANQKPPSAEFKVVKAQIQEQKLLQRLLDDRKSTVEVIKREGEKIATTAEPADKVKILKQLSLLDSRWEALLNKAETRNRQLEGISVVAQQFHETLEPLNEWLTTIEKRLVNCEPIGTQASKLEEQIAQHKALEDDIINHNKHLHQAVSIGQSLKVLSSREDKDMVQSKLDFSQVWYIEIQEKSHSRSELLQQALCNAKIFGEDEVELMNWLNEVHDKLSKLSVQDYSTEGLWKQQSELRVLQEDILLRKQNVDQALLNGLELLKQTTGDEVLIIQDKLEAIKARYKDITKLSTDVAKTLEQALQLARRLHSTHEELCTWLDKVEVELLSYETQVLKGEEASQAQMRPKELKKEAKNNKALLDSLNEVSSALLELVPWRAREGLEKMVAEDNERYRLVSDTITQKVEEIDAAILRSQQFDQAADAELSWITETEKKLMSLGDIRLEQDQTSAQLQVQKTFTMEILRHKDIIDDLVKSGHKIMTACSEEEKQSMKKKLDKVLKNYDTICQINSERYLQLERAQSLVNQFWETYEELWPWLTETQSIISQLPAPALEYETLRQQQEEHRQLRELIAEHKPHIDKMNKTGPQLLELSPGEGFSIQEKYVAADTLYSQIKEDVKKRAVALDEAISQSTQFHDKIDQILESLERIVERLRQPPSISAEVEKIKEQISENKNVSVDMEKLQPLYETLKQRGEEMIARSGGTDKDISAKAVQDKLDQMVFIWENIHTLVEEREAKLLDVMELAEKFWCDHMSLIVTIKDTQDFIRDLEDPGIDPSVVKQQQEAAETIREEIDGLQEELDIVINLGSELIAACGEPDKPIVKKSIDELNSAWDSLNKAWKDRIDKLEEAMQAAVQYQDGLQAVFDWVDIAGGKLASMSPIGTDLETVKQQIEELKQFKSEAYQQQIEMERLNHQAELLLKKVTEESDKHTVQDPLMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEALNLAMEFHNSLQDFINWLTQAEQTLNVASRPSLILDTVLFQIDEHKVFANEVNSHREQIIELDKTGTHLKYFSQKQDVVLIKNLLISVQSRWEKVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLADDNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLPDDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRFPASSLYPSGSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAYKPITDADKIEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNMELREKFILADGASQGMAAFRPRGRRSRPSSRGASPNRSTSVSSQAAQAASPQVPATTTPKILHPLTRNYGKPWLTNSKMSTPCKAAECSDFPVPSAEGTPIQGSKLRLPGYLSGKGFHSGEDSGLITTAAARVRTQFADSKKTPSRPGSRAGSKAGSRASSRRGSDASDFDISEIQSVCSDVETVPQTHRPTPRAGSRPSTAKPSKIPTPQRKSPASKLDKSSKR

"
     misc_feature    681..989
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Calponin homology domain; actin-binding domain
                     which may be present as a single copy or in tandem repeats
                     (which increases binding affinity). The CH domain is found
                     in cytoskeletal and signal transduction proteins,
                     including actin-binding proteins like...; Region: CH;
                     cd00014"
                     /db_xref="CDD:237981"
     misc_feature    order(681..686,690..698,705..707,714..716,873..878,
                     885..887,894..896,900..902,945..950,957..962,966..971,
                     978..983)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="putative actin binding surface [polypeptide
                     binding]; other site"
                     /db_xref="CDD:237981"
     misc_feature    1029..1340
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Calponin homology domain; actin-binding domain
                     which may be present as a single copy or in tandem repeats
                     (which increases binding affinity). The CH domain is found
                     in cytoskeletal and signal transduction proteins,
                     including actin-binding proteins like...; Region: CH;
                     cd00014"
                     /db_xref="CDD:237981"
     misc_feature    order(1029..1034,1038..1046,1053..1055,1062..1064,
                     1221..1226,1233..1235,1242..1244,1248..1250,1296..1301,
                     1308..1313,1317..1322,1329..1334)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="putative actin binding surface [polypeptide
                     binding]; other site"
                     /db_xref="CDD:237981"
     misc_feature    2397..2672
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats; Region: SPEC; smart00150"
                     /db_xref="CDD:197544"
     misc_feature    2676..3224
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    2985..3002
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    4155..4649
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cl02488"
                     /db_xref="CDD:243054"
     misc_feature    4437..4454
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    6087..6776
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    6441..6458
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    6804..7427
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cl02488"
                     /db_xref="CDD:243054"
     misc_feature    7107..7124
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    7857..8522
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    8184..8201
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    8862..9422
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    9174..9191
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    9183..9818
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    9495..9512
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    9840..10457
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    order(10152..10166,10170..10172)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    10161..10808
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    10485..10502
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    10503..11144
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    10812..10829
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    10824..11471
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    11139..11156
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    11475..12122
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    order(11793..11795,11799..11813)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    11808..12449
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    12120..12137
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    12462..13115
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    12783..12800
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    12792..13445
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    13110..13127
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    13128..13772
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    order(13440..13454,13458..13460)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    13452..14099
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    13767..13784
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    14103..14756
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    14421..14438
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    14769..15407
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    15075..15092
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    15084..15827
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    order(15402..15410,15423..15431)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="linker region; other site"
                     /db_xref="CDD:238103"
     misc_feature    15918..16106
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     Region: EFh; cd00051"
                     /db_xref="CDD:238008"
     misc_feature    15936..16103
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="EF-hand domain pair; Region: EF_hand_5; pfam13499"
                     /db_xref="CDD:222177"
     misc_feature    order(15945..15947,15951..15953,15957..15959,15978..15980,
                     16053..16055,16059..16061,16065..16067,16086..16088)
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Ca2+ binding site [ion binding]; other site"
                     /db_xref="CDD:238008"
     misc_feature    16143..16379
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /note="Growth-Arrest-Specific Protein 2 Domain; Region:
                     GAS2; smart00243"
                     /db_xref="CDD:128539"
     STS             8022..8122
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /standard_name="EST4G12"
                     /db_xref="UniSTS:263370"
     variation       15875
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /replace="c"
                     /replace="t"
                     /db_xref="dbSNP:17292790"
     variation       16096
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /replace="c"
                     /replace="t"
                     /db_xref="dbSNP:1049797"
     STS             17005..17173
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /standard_name="D6S1122E"
                     /db_xref="UniSTS:21947"
     STS             17625..17760
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /standard_name="D6S1159E"
                     /db_xref="UniSTS:26810"
     STS             17794..17912
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
                     /standard_name="RH15996"
                     /db_xref="UniSTS:55081"
     polyA_signal    17813..17818
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
     polyA_site      17836
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
     polyA_signal    18058..18063
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
     polyA_site      18078
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
     polyA_site      18087
                     /gene="DST"
                     /gene_synonym="BP240; BPA; BPAG1; CATX-15; CATX15;
                     D6S1101; DMH; DT; HSAN6; MACF2"
ORIGIN

ctccagccccgccgtcgagtcgccctcgcctcgcccgcaccatgatcgccgcggctttcctcgtcttgctgagaccctacagcatccaatgtgccctcttcctcttgttgcttctgctgggcaccatcgccaccatcgtcttcttctgctgctggcaccgcaagctccagaaagggaggcatccgatgaaatcggtcttctcgggtcgttcaagaagccgagatgctgttttgagatcgcaccactttcgttctgagggattcagagcaagccctcggcatcttagaagacgagttgcagcggccgcagctgcccgtctggaagaagtgaagcccgtggtggaagttcaccatcagagtgagcaagaaacttcagtgaggaaacgaagaatcaagaagagcagccgagtccagccagaattttatcactctgttcaaggtgcttcaatcaggaggcctagttccgggaacgcgtcctatcgctgctctatgtcttcctctgcggatttttccgatgaggatgatttcagccagaaatctggctccgcatccccagctccgggagacaccttaccctggaatttgcctaaacatgagagatcgaaaagaaagattcaagggggctcagtgctggaccctgccgagagggcagtgcttcggatagcagatgaacgggacaaagttcagaagaaaacatttacaaaatggataaatcagcatctcatgaaggttcgaaaacatgtgaatgatctctatgaagacttaagggatggacacaatttgatttctcttttagaggttctttcaggagataccttgcccagagaaaaaggtcggatgcgttttcacagactacagaatgtacaaattgcacttgactatttgaaaagacgccaggtgaaattagtgaatattagaaatgatgacataacagatggaaatcccaaattgactttgggattaatctggacaataattttgcactttcagatatctgatatccatgttactggagagtcagaggatatgtctgcaaaagagagattgctactctggacgcagcaggcaacagagggttatgctggaattcggtgtgaaaatttcactacctgctggagagatggaaaattatttaatgccatcattcataaatacaggccggacctgatagatatgaatactgttgctgttcaaagcaaccttgcaaatttagagcatgctttttacgtagcagaaaaaattggtgttataagacttctggaccctgaagatgtcgatgtctcctcacctgatgaaaaatcagtaataacttacgtgtcatctctctatgatgcatttcctaaagtccctgaaggtggggaaggcattggtgcaaatgatgttgaagtcaaatggattgaataccagaatatggtgaactacctcatccaatggattagacaccatgtgaccacaatgtctgagagaacatttcctaataatcctgtagaactgaaggcactttataatcagtatttacagtttaaagaaacagaaattccaccaaaggagacagaaaaatcaaaaattaaacgtctatataaattattagagatttggatagaatttggacgcatcaagctccttcaaggttatcatccaaatgacatagaaaaagagtgggggaaactcattattgccatgctagagagagagaaggcccttcgtccagaagtagaaaggttagaaatgttgcaacagattgccaacagagttcagagggacagtgtcatctgtgaagacaaactgattcttgctggaaatgctcttcagtctgattctaaaagattagaatcaggagtgcagtttcagaatgaagcagaaattgctgggtatatacttgaatgtgagaaccttttacgccagcatgtaattgatgtacagattcttattgatggaaaatactaccaggcagatcaattggtacagagggttgcaaaactgcgtgacgaaattatggccttaaggaacgaatgttcttctgtgtacagcaaaggacgcatactgacaacagaacagacaaagctcatgatatcaggaatcactcaaagtttaaactcaggatttgcacagaccttacaccctagtctgacctcagggctgacccagagtttaacaccttccctaacctcttctagtatgacttctggcctgtcatcagggatgacttcccgcctgactccatctgtcactccagcttatacacctggtttcccatcaggattagttccaaatttcagttcaggagtagagccaaattcattgcaaactttgaagttgatgcagatccgaaaaccccttctaaagtcttctttgctggatcaaaatttaacagaagaagaaatcaatatgaaatttgttcaggatcttttgaattgggttgatgagatgcaggtacaactggaccgcactgagtggggctcagatttgccaagtgttgaaagccatttagaaaatcataaaaatgttcatagagctattgaagaatttgaatctagtctcaaagaagctaaaatcagtgagattcaaatgacagcacctcttaaactgacttatgcagaaaagttgcacagattagagagtcagtatgcaaaactcttgaatacatccaggaatcaagaacggcaccttgatacactccataattttgtaagtcgtgcgactaatgaacttatttggttgaatgaaaaagaagaggaggaagttgcttatgactggagtgagagaaacaccaacatagctaggaaaaaagattatcatgctgaattaatgagagaacttgatcaaaaggaagaaaatattaaatcagttcaggagatagcagagcagctacttctagaaaatcatccagcccggttaactattgaggcctacagagcggcaatgcagacgcagtggagctggatcttacagctctgccagtgtgtggagcagcacataaaggagaacacagcgtatttcgagtttttcaatgatgccaaagaagctactgattacttaaggaatctaaaagatgccattcagcggaagtacagctgtgatagatcaagcagcattcacaagctagaagaccttgttcaggaatcaatggaagagaaagaagaacttctgcagtacaaaagcactatagcaaacctaatgggaaaagcaaaaacaataattcaactgaagccaaggaattctgactgtccactcaaaacttctattccgatcaaagctatctgtgactacagacaaattgagataaccatttacaaagacgatgaatgtgttttggcgaataactctcatcgtgctaaatggaaggtcattagtcctactgggaatgaggctatggtcccatctgtgtgcttcaccgttcctccaccaaacaaagaagcggtggaccttgccaacagaattgagcaacagtatcagaatgtcctgactctttggcatgagtctcacataaacatgaagagtgtagtatcctggcattatctcatcaatgaaattgatagaattcgagctagcaatgtggcttcaataaagacaatgctacctggtgaacatcagcaagttctaagtaatctacaatctcgttttgaagattttctggaagatagccaggaatcccaagtcttttcaggctcagatataacacaactggaaaaggaggttaatgtatgtaagcagtattatcaagaacttcttaaatctgcagaaagagaggagcaagaggaatcagtttataatctctacatctctgaagttcgaaacattagacttcggttagagaactgtgaagatcggctgattagacagattcgaactcccctggaaagagatgatttgcatgaaagtgtgttcagaatcacagaacaggagaaactaaagaaagagctggaacgacttaaagatgatttgggaacaatcacaaataagtgtgaggagtttttcagtcaagcagcagcctcttcatcagtccctaccctacgatcagagcttaatgtggtccttcagaacatgaaccaagtctattctatgtcttccacttacatagataagttgaaaactgttaacttggtgttaaaaaacactcaagctgcagaagccctcgtaaaactctatgaaactaaactgtgtgaagaagaagcagttatagctgacaagaataatattgagaatctaataagtactttaaagcaatggagatctgaagtagatgaaaagagacaggtattccatgccttagaggatgagttgcagaaagctaaagccatcagtgatgaaatgtttaaaacgtataaagaacgggaccttgattttgactggcacaaagaaaaagcagatcaattagttgaaaggtggcaaaatgttcatgtgcagattgacaacaggttacgggacttagagggcattggcaaatcactgaagtactacagagacacttaccatcctttagatgattggatccagcaggttgaaactactcagagaaagattcaggaaaatcagcctgaaaatagtaaaaccctagccacacagttgaatcaacagaagatgctggtgtccgaaatagaaatgaaacagagcaaaatggacgagtgtcaaaaatatgcagaacagtactcagctacagtgaaggactatgaattacaaacaatgacctaccgggccatggtagattcacaacaaaaatctccagtgaaacgccgaagaatgcagagttcagcagatctcattattcaagagttcatggacctaaggactcgatatactgccctggtcactctcatgacacaatatattaaatttgctggtgattcattgaagaggctggaagaggaggagaagtcactggaagaagaaaagaaagaacatgttgaaaaagccaaagaattgcaaaaatgggtatcaaatatcagcaagacattgaaagatgcagagaaggctggaaaacctcccttctctaagcaaaag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