GGRNA | NM_001099431.1

LOCUS       NM_001099431             883 bp    mRNA    linear   PRI 19-MAY-2013
DEFINITION  Homo sapiens C-type lectin domain family 1, member B (CLEC1B),
            transcript variant 2, mRNA.
ACCESSION   NM_001099431
VERSION     NM_001099431.1  GI:153252047
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Manne,B.K., Getz,T.M., Hughes,C.E., Alshehri,O., Dangelmaier,C.,
            Naik,U.P., Watson,S.P. and Kunapuli,S.P.
  TITLE     Fucoidan is a novel platelet agonist for the C-type lectin-like
            receptor 2 (CLEC-2)
  JOURNAL   J. Biol. Chem. 288 (11), 7717-7726 (2013)
   PUBMED   23341451
  REMARK    GeneRIF: fucoidan is a novel CLEC-2 receptor agonist that activates
            platelets through a SFK-dependent signaling pathway
REFERENCE   2  (bases 1 to 883)
  AUTHORS   Hughes,C.E., Sinha,U., Pandey,A., Eble,J.A., O'Callaghan,C.A. and
            Watson,S.P.
  TITLE     Critical Role for an acidic amino acid region in platelet signaling
            by the HemITAM (hemi-immunoreceptor tyrosine-based activation
            motif) containing receptor CLEC-2 (C-type lectin receptor-2)
  JOURNAL   J. Biol. Chem. 288 (7), 5127-5135 (2013)
   PUBMED   23264619
  REMARK    GeneRIF: Dimerization of two phosphorylated CLEC-2 molecules leads
            to recruitment of the tyrosine kinase Syk via its tandem SH2
            domains and initiation of a downstream signaling cascade
REFERENCE   3  (bases 1 to 883)
  AUTHORS   Parguina,A.F., Alonso,J., Rosa,I., Velez,P., Gonzalez-Lopez,M.J.,
            Guitian,E., Ebble,J.A., Loza,M.I. and Garcia,A.
  TITLE     A detailed proteomic analysis of rhodocytin-activated platelets
            reveals novel clues on the CLEC-2 signalosome: implications for
            CLEC-2 signaling regulation
  JOURNAL   Blood 120 (26), E117-E126 (2012)
   PUBMED   23053573
  REMARK    GeneRIF: A differential proteomic analysis of basal and
            rhodocytin-activated platelets with the aim of providing novel
            clues on CLEC-2 signaling regulation.
REFERENCE   4  (bases 1 to 883)
  AUTHORS   Wang,L., Ren,S., Zhu,H., Zhang,D., Hao,Y., Ruan,Y., Zhou,L.,
            Lee,C., Qiu,L., Yun,X. and Xie,J.
  TITLE     Structural and functional conservation of CLEC-2 with the
            species-specific regulation of transcript expression in evolution
  JOURNAL   Glycoconj. J. 29 (5-6), 335-345 (2012)
   PUBMED   22740230
  REMARK    GeneRIF: The glycosylation sites (N120 and N134) are necessary for
            the surface expression of CLEC-2.
REFERENCE   5  (bases 1 to 883)
  AUTHORS   Fei,M., Zhou,L., Xie,J., Ruan,Y., Xu,J., He,S., Shen,H., Hu,Y.,
            Ren,S. and Ruan,C.
  TITLE     The production of soluble C-type lectin-like receptor 2 is a
            regulated process
  JOURNAL   Glycoconj. J. 29 (5-6), 315-321 (2012)
   PUBMED   22736279
  REMARK    GeneRIF: Shedding of CLEC-2 from the cell surface may reflect
            increased expression of this membrane protein and serve as a marker
            of disease activity.
REFERENCE   6  (bases 1 to 883)
  AUTHORS   Chaipan,C., Soilleux,E.J., Simpson,P., Hofmann,H., Gramberg,T.,
            Marzi,A., Geier,M., Stewart,E.A., Eisemann,J., Steinkasserer,A.,
            Suzuki-Inoue,K., Fuller,G.L., Pearce,A.C., Watson,S.P., Hoxie,J.A.,
            Baribaud,F. and Pohlmann,S.
  TITLE     DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1
            capture by platelets
  JOURNAL   J. Virol. 80 (18), 8951-8960 (2006)
   PUBMED   16940507
  REMARK    GeneRIF: platelets capture and transfer infectious HIV-1 via
            DC-SIGN and CLEC-2, thereby possibly facilitating HIV-1
            dissemination in infected patients
REFERENCE   7  (bases 1 to 883)
  AUTHORS   Suzuki-Inoue,K., Fuller,G.L., Garcia,A., Eble,J.A., Pohlmann,S.,
            Inoue,O., Gartner,T.K., Hughan,S.C., Pearce,A.C., Laing,G.D.,
            Theakston,R.D., Schweighoffer,E., Zitzmann,N., Morita,T.,
            Tybulewicz,V.L., Ozaki,Y. and Watson,S.P.
  TITLE     A novel Syk-dependent mechanism of platelet activation by the
            C-type lectin receptor CLEC-2
  JOURNAL   Blood 107 (2), 542-549 (2006)
   PUBMED   16174766
REFERENCE   8  (bases 1 to 883)
  AUTHORS   Watson,A.A. and O'Callaghan,C.A.
  TITLE     Crystallization and X-ray diffraction analysis of human CLEC-2
  JOURNAL   Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 (PT 12),
            1094-1096 (2005)
   PUBMED   16511244
  REMARK    GeneRIF: X-ray diffraction analysis of human CLEC-2
REFERENCE   9  (bases 1 to 883)
  AUTHORS   Sobanov,Y., Bernreiter,A., Derdak,S., Mechtcheriakova,D.,
            Schweighofer,B., Duchler,M., Kalthoff,F. and Hofer,E.
  TITLE     A novel cluster of lectin-like receptor genes expressed in
            monocytic, dendritic and endothelial cells maps close to the NK
            receptor genes in the human NK gene complex
  JOURNAL   Eur. J. Immunol. 31 (12), 3493-3503 (2001)
   PUBMED   11745369
REFERENCE   10 (bases 1 to 883)
  AUTHORS   Colonna,M., Samaridis,J. and Angman,L.
  TITLE     Molecular characterization of two novel C-type lectin-like
            receptors, one of which is selectively expressed in human dendritic
            cells
  JOURNAL   Eur. J. Immunol. 30 (2), 697-704 (2000)
   PUBMED   10671229
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC029554.1, AC091814.10 and AY358599.1.
            
            Summary: Natural killer (NK) cells express multiple
            calcium-dependent (C-type) lectin-like receptors, such as CD94
            (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact
            with major histocompatibility complex class I molecules and either
            inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a
            C-type lectin-like receptor expressed in myeloid cells and NK cells
            (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan
            2011].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1, resulting in a
            shorter protein (isoform b), compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647321.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           ERS025088 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               BC029554.1         71-207
            138-257             BC029554.1         209-328
            258-258             AC091814.10        11405-11405
            259-291             BC029554.1         330-362
            292-292             AC091814.10        13196-13196
            293-883             AY358599.1         373-963
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.2"
     gene            1..883
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /note="C-type lectin domain family 1, member B"
                     /db_xref="GeneID:51266"
                     /db_xref="HGNC:24356"
                     /db_xref="MIM:606783"
     exon            1..264
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    135..137
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /note="upstream in-frame stop codon"
     CDS             201..791
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /note="isoform b is encoded by transcript variant 2;
                     C-type lectin-like receptor-2; CLEC-2; C-type lectin-like
                     receptor 2"
                     /codon_start=1
                     /product="C-type lectin domain family 1 member B isoform
                     b"
                     /protein_id="NP_001092901.1"
                     /db_xref="GI:153252048"
                     /db_xref="CCDS:CCDS41751.1"
                     /db_xref="GeneID:51266"
                     /db_xref="HGNC:24356"
                     /db_xref="MIM:606783"
                     /translation="

MQDEDGYITLNIKTRKPALISAVMQRNYLQGENENRTGTLQQLAKRFCQYVVKQSELKGTFKGHKCSPCDTNWRYYGDSCYGFFRHNLTWEESKQYCTDMNATLLKIDNRNIVEYIKARTHLIRWVGLSRQKSNEVWKWEDGSVISENMFEFLEDGKGNMNCAYFHNGKMHPTFCENKHYLMCERKAGMTKVDQLP

"
     misc_feature    405..755
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /note="C-type lectin-like domain (CTLD) of the type found
                     in natural killer cell receptors (NKRs); Region:
                     CLECT_NK_receptors_like; cd03593"
                     /db_xref="CDD:153063"
     misc_feature    order(567..569,651..653,705..707,711..716)
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /note="ligand binding surface [chemical binding]; other
                     site"
                     /db_xref="CDD:153063"
     variation       258
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:612593"
     exon            265..384
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /inference="alignment:Splign:1.39.8"
     variation       292
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /replace="a"
                     /replace="g"
                     /db_xref="dbSNP:583903"
     exon            385..539
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /inference="alignment:Splign:1.39.8"
     exon            540..646
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /inference="alignment:Splign:1.39.8"
     exon            647..873
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /inference="alignment:Splign:1.39.8"
     variation       802
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
                     /replace="a"
                     /replace="t"
                     /db_xref="dbSNP:15635"
     polyA_signal    837..842
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
     polyA_site      859
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
     polyA_site      873
                     /gene="CLEC1B"
                     /gene_synonym="1810061I13Rik; CLEC2; CLEC2B; PRO1384;
                     QDED721"
ORIGIN

gtccatgtatctctgagcagctatgaagaagcttcctggaaaacaataagcaaaggaaaacaaatgtgtcccatctcacatggttctaccctactaaagacaggaagatcataaactgacagatactgaaattgtaaagttggaaactacattttgcaaagtcattgaactctgagctcagttgcagtactcgggaagccatgcaggatgaagatggatacatcaccttaaatattaaaactcggaaaccagctctcatctccgctgtcatgcagcgcaattacctacaaggtgagaatgaaaatcgcacaggaactctgcaacaattagcaaagcgcttctgtcaatatgtggtaaaacaatcagaactaaagggcactttcaaaggtcataaatgcagcccctgtgacacaaactggagatattatggagatagctgctatgggttcttcaggcacaacttaacatgggaagagagtaagcagtactgcactgacatgaatgctactctcctgaagattgacaaccggaacattgtggagtacatcaaagccaggactcatttaattcgttgggtcggattatctcgccagaagtcgaatgaggtctggaagtgggaggatggctcggttatctcagaaaatatgtttgagtttttggaagatggaaaaggaaatatgaattgtgcttattttcataatgggaaaatgcaccctaccttctgtgagaacaaacattatttaatgtgtgagaggaaggctggcatgaccaaggtggaccaactaccttaatgcaaagaggtggacaggataacacagataagggctttattgtacaataaaagatatgtatgaatgcatcagtagctgaaaaaaaaaaaaaa

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