2025-05-09 16:25:24, GGRNA : RefSeq release 60 (20130726)
LOCUS NM_013233 3288 bp mRNA linear PRI 17-APR-2013 DEFINITION Homo sapiens serine threonine kinase 39 (STK39), mRNA. ACCESSION NM_013233 VERSION NM_013233.2 GI:115430251 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 3288) AUTHORS Ponce-Coria,J., Gagnon,K.B. and Delpire,E. TITLE Calcium-binding protein 39 facilitates molecular interaction between Ste20p proline alanine-rich kinase and oxidative stress response 1 monomers JOURNAL Am. J. Physiol., Cell Physiol. 303 (11), C1198-C1205 (2012) PUBMED 23034389 REMARK GeneRIF: SPAK and OSR1, which are oftencoexpressed in cells can form functional heterodimers. REFERENCE 2 (bases 1 to 3288) AUTHORS Vavolizza,R.D., Schmeidler,J., Ramoz,N., Buxbaum,J.D., Smith,C.J. and Silverman,J.M. TITLE The effect of an autism-associated polymorphism in the STK39 gene on the autism symptom domains JOURNAL J Autism Dev Disord 42 (2), 319-320 (2012) PUBMED 21442361 REMARK GeneRIF: our results suggest no significant assocation between any of the core autism symptom domains or the four additional previously identified familial features and the rs1807984 SNP on the STK39 gene. REFERENCE 3 (bases 1 to 3288) AUTHORS Yan,Y., Laroui,H., Ingersoll,S.A., Ayyadurai,S., Charania,M., Yang,S., Dalmasso,G., Obertone,T.S., Nguyen,H., Sitaraman,S.V. and Merlin,D. TITLE Overexpression of Ste20-related proline/alanine-rich kinase exacerbates experimental colitis in mice JOURNAL J. Immunol. 187 (3), 1496-1505 (2011) PUBMED 21705622 REMARK GeneRIF: SPAK increases intestinal epithelial permeability; both SPAK-transfected Caco2-cells and SPAK transgenic mice exhibit loss of intestinal barrier function and homeostasis in inflammatory bowel disease. REFERENCE 4 (bases 1 to 3288) AUTHORS Richardson,C., Sakamoto,K., de los Heros,P., Deak,M., Campbell,D.G., Prescott,A.R. and Alessi,D.R. TITLE Regulation of the NKCC2 ion cotransporter by SPAK-OSR1-dependent and -independent pathways JOURNAL J. Cell. Sci. 124 (PT 5), 789-800 (2011) PUBMED 21321328 REMARK GeneRIF: Data demonstrate that hypotonic low-chloride conditions that activate the WNK1-SPAK and OSR1 pathway promote phosphorylation of NKCC2 isoforms. REFERENCE 5 (bases 1 to 3288) AUTHORS Yang,D., Li,Q., So,I., Huang,C.L., Ando,H., Mizutani,A., Seki,G., Mikoshiba,K., Thomas,P.J. and Muallem,S. TITLE IRBIT governs epithelial secretion in mice by antagonizing the WNK/SPAK kinase pathway JOURNAL J. Clin. Invest. 121 (3), 956-965 (2011) PUBMED 21317537 REMARK GeneRIF: IRBIT opposes the effects of WNKs and SPAK by recruiting PP1 to the complex to dephosphorylate CFTR and NBCe1-B, restoring their cell surface expression, in addition to stimulating their activities REFERENCE 6 (bases 1 to 3288) AUTHORS Moriguchi,T., Urushiyama,S., Hisamoto,N., Iemura,S., Uchida,S., Natsume,T., Matsumoto,K. and Shibuya,H. TITLE WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1 JOURNAL J. Biol. Chem. 280 (52), 42685-42693 (2005) PUBMED 16263722 REMARK GeneRIF: WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to cation-chloride-coupled cotransporters REFERENCE 7 (bases 1 to 3288) AUTHORS Piechotta,K., Garbarini,N., England,R. and Delpire,E. TITLE Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase JOURNAL J. Biol. Chem. 278 (52), 52848-52856 (2003) PUBMED 14563843 REFERENCE 8 (bases 1 to 3288) AUTHORS Dowd,B.F. and Forbush,B. TITLE PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1) JOURNAL J. Biol. Chem. 278 (30), 27347-27353 (2003) PUBMED 12740379 REMARK GeneRIF: roles in phosphorylation and activation of the Na-K-Cl cotransporter (NKCC1) REFERENCE 9 (bases 1 to 3288) AUTHORS Qi,H., Labrie,Y., Grenier,J., Fournier,A., Fillion,C. and Labrie,C. TITLE Androgens induce expression of SPAK, a STE20/SPS1-related kinase, in LNCaP human prostate cancer cells JOURNAL Mol. Cell. Endocrinol. 182 (2), 181-192 (2001) PUBMED 11514053 REFERENCE 10 (bases 1 to 3288) AUTHORS Johnston,A.M., Naselli,G., Gonez,L.J., Martin,R.M., Harrison,L.C. and DeAizpurua,H.J. TITLE SPAK, a STE20/SPS1-related kinase that activates the p38 pathway JOURNAL Oncogene 19 (37), 4290-4297 (2000) PUBMED 10980603 COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF099989.1, AC016723.11 and BF516344.1. On Sep 30, 2006 this sequence version replaced gi:7019542. Summary: This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF099989.1, AF030403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support ERS025081, ERS025082 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-51 AF099989.1 2-52 52-309 AF099989.1 65-322 310-2228 AF099989.1 329-2247 2229-3119 AC016723.11 170127-171017 c 3120-3288 BF516344.1 1-169 c FEATURES Location/Qualifiers source 1..3288 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="2" /map="2q24.3" gene 1..3288 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="serine threonine kinase 39" /db_xref="GeneID:27347" /db_xref="HGNC:17717" /db_xref="MIM:607648" exon 1..368 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" STS 133..1825 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /db_xref="UniSTS:482333" CDS 161..1798 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /EC_number="2.7.11.1" /note="serine threonine kinase 39 (STE20/SPS1 homolog, yeast); Ste20-like protein kinase; small intestine SPAK-like kinase; proline-alanine-rich STE20-related kinase; ste-20-related kinase; serine/threonine-protein kinase 39" /codon_start=1 /product="STE20/SPS1-related proline-alanine-rich protein kinase" /protein_id="NP_037365.2" /db_xref="GI:115430252" /db_xref="CCDS:CCDS42770.1" /db_xref="GeneID:27347" /db_xref="HGNC:17717" /db_xref="MIM:607648" /translation="
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
" misc_feature 341..1171 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="Catalytic domain of the Protein Serine/Threonine Kinases, Oxidative stress response kinase and Ste20-related proline alanine-rich kinase; Region: STKc_OSR1_SPAK; cd06610" /db_xref="CDD:173726" misc_feature 347..1171 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="Serine/Threonine protein kinases, catalytic domain; Region: S_TKc; smart00220" /db_xref="CDD:197582" misc_feature order(365..379,389..391,428..430,434..436,524..526, 572..583,590..595,602..604,734..736,740..751,755..757, 788..790,797..799,854..865,869..871,953..955,980..982) /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="active site" /db_xref="CDD:173726" misc_feature order(365..379,389..391,428..430,434..436,524..526, 572..583,590..595,602..604,740..742,746..751,755..757, 788..790) /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="ATP binding site [chemical binding]; other site" /db_xref="CDD:173726" misc_feature order(374..379,734..736,740..748,797..799,854..865, 869..871,953..955,980..982) /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="substrate binding site [chemical binding]; other site" /db_xref="CDD:173726" misc_feature order(785..826,845..871) /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="activation loop (A-loop); other site" /db_xref="CDD:173726" misc_feature 1085..1087 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine, by PKC/PRKCQ; propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); phosphorylation site" misc_feature 1085..1087 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="phosphorylation site" /citation=[10] /db_xref="HPRD:02710" misc_feature 1127..1129 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="phosphorylation site" /citation=[10] /db_xref="HPRD:02710" misc_feature 1205..1207 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine; propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); acetylation site" misc_feature 1238..1258 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="non-experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); Region: Nuclear localization signal (Potential)" misc_feature 1268..1270 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); phosphorylation site" misc_feature 1268..1270 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="phosphorylation site" misc_feature 1271..1273 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); phosphorylation site" misc_feature 1313..1315 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); phosphorylation site" misc_feature 1313..1315 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="phosphorylation site" misc_feature 1319..1333 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9UEW8.3); Region: Caspase cleavage related site" misc_feature 1514..1609 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /note="Oxidative-stress-responsive kinase 1 C terminal; Region: OSR1_C; pfam12202" /db_xref="CDD:204847" exon 369..481 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 482..590 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 591..732 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 733..788 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 789..898 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 899..1000 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1001..1134 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1135..1183 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1184..1249 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" variation 1220 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /replace="a" /replace="c" /db_xref="dbSNP:1802105" exon 1250..1372 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1373..1402 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1403..1465 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1466..1536 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1537..1589 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1590..1658 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1659..1723 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" exon 1724..3274 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /inference="alignment:Splign:1.39.8" STS 1797..1952 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /standard_name="RH68427" /db_xref="UniSTS:31029" polyA_site 1979 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" variation 2452 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /replace="a" /replace="g" /db_xref="dbSNP:1061471" variation 2682 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /replace="g" /replace="t" /db_xref="dbSNP:3769428" STS 2855..3023 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /standard_name="RH99072" /db_xref="UniSTS:85155" variation 3065 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" /replace="a" /replace="g" /db_xref="dbSNP:3769429" polyA_signal 3250..3255 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" polyA_site 3274 /gene="STK39" /gene_synonym="DCHT; PASK; SPAK" ORIGIN
gctccctcccctcctagctggcttcggcggggacggcggcggcggcggcgggcggggagggcgtgcgccggccgagaggtgtcggcggcgaggcaaaggaagtttcaagtggaaggtcgtccgtcggccggcgcgtcctcctgctctcctccgcagcatcatggcggagccgagcggctcgcccgtgcacgtccagcttccccagcaggcggccccggtgacagcggcggcggcggcggccccggcggccgcgacagcagcgccggccccggcagctcccgcggccccggccccggccccggccccggcggcacaggctgtcggctggcccatctgcagggacgcgtacgagctgcaggaggttatcggcagtggagctactgctgtggttcaggcagccctatgcaaacccaggcaagaacgtgtagcaataaaacggatcaacttggaaaaatgccagaccagtatggatgaactattaaaagaaattcaagccatgagtcagtgcagccatcccaacgtagtgacctattacacctcttttgtggtcaaagatgaactttggctggtcatgaaattactaagtggaggttcaatgttggatatcataaaatacattgtcaaccgaggagaacacaagaatggagttctggaagaggcaataatagcaacaattcttaaagaggttttggaaggcttagactatctacacagaaacggtcagattcacagggatttgaaagctggtaatattcttctgggtgaggatggttcagtacaaatagcagattttggggtaagtgcgttcctagcaacagggggtgatgttacccgaaataaagtaagaaaaacattcgttggcaccccatgttggatggctcctgaagtcatggaacaggtgagaggctatgacttcaaggctgacatgtggagttttggaataactgccattgaattagcaacaggagcagcgccttatcacaaatatcctcccatgaaagtgttaatgttgactttgcaaaatgatccacccactttggaaacaggggtagaggataaagaaatgatgaaaaagtacggcaagtcctttagaaaattactttcactgtgtcttcagaaagatccttccaaaaggcccacagcagcagaacttttaaaatgcaaattcttccagaaagccaagaacagagagtacctgattgagaagctgcttacaagaacaccagacatagcccaaagagccaaaaaggtaagaagagttcctgggtcaagtggtcaccttcataaaaccgaagacggggactgggagtggagtgacgacgagatggatgagaagagcgaagaagggaaagcagctttttctcaggaaaagtcacgaagagtaaaagaagaaaatccagagattgcagtgagtgccagcaccatccccgaacaaatacagtccctctctgtgcacgactctcagggcccacccaatgctaatgaagactacagagaagcttcttcttgtgccgtgaacctcgttttgagattaagaaactccagaaaggaacttaatgacatacgatttgagtttactccaggaagagatacagcagatggtgtatctcaggagctcttctctgctggcttggtggatggtcacgatgtagttatagtggctgctaatttacagaagattgtagatgatcccaaagctttaaaaacattgacatttaagttggcttctggctgtgatgggtcggagattcctgatgaagtgaagctgattgggtttgctcagttgagtgtcagctgatgtatgtcccttgatgtcaccctgatctgtcatgccccaccgccacccctactcccttcaaccctccctctttctgcccatttcctcccaccccctcactcccatttcctagcaaaatcagaagattgtgaagaggccggcttcaacaaaatgggataaaaaaataattttttaaaacttacaacactccgagttctgctttattctctagcaatccacagtacaagaacaagcaaatgccacagctgcacgactgttgctcatttttccaaaagctatttaatattcttagcaatcaatttggatatcccttaagtgaaaagaatctgaaatacactcaggtggtcttatttattggcaacaaaaggaattttctatccagaagcctatttctcctttcattgttgttatttctgttataatactttaattgtacatctgacaatactgcctcttttatgttgtatttagaaattaatatacttataaaattaagatttattagccaaacttgaattctagttttaaaactgactgtgaattttatttttcatatatttatgcattacacaccttagctataagaaaaaaagggttttgattatatgcttcttgcagttaatctcgttatttaaacaaaaagttttgggtctgtctttggagtatttgtaacttctaaattttgaaatgactgaattaggaatttggatgcttattcttttagtctgtttgcctaaaaaccaatttacaatctgactgtctcttgggagagggaggtgccttgcaaactttcacattaagaatgtgcctgaggctgctttactctggaatagtctcagatctaaaatttcctctatataaggtggcatatgttaagttttgcttcattggaccgtttagaatgctatgtaaaatgttgccattctgttagattgctaactatatacccatctctgatttggctctccttaagtgataggatttgttattctaaaggtgataaacttgaaaatatcagaatctgagttttacttgaaattttgcagaatacccaggtggagtgaaaattggaagggttttgtgcaatgactaaaaggtaaaacgctgttaaggttcaagaatcaatactttcaacccaagtagccctctgcttgactgtatattatggaactagtaaaccttaggattttgaaaattggagtctaatctttcaaggaggtgggctcccaggatggtaccattgctctttcctagctaaccctagatatggcagctctttaatgtacttcaaaaagcaaatatatattactaaggaaaaaaagttatttataattgccttgtcataattgttaaggtgttctagagccatttgcatacaatttaatgtaatttcattccattctattgtttacacaacgattactcgaagatgactgcaaaggtaaaaggaaaataaaagtgtattgcacaatgagaaaaaaaaaaaaaaa
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ANNOTATIONS from NCBI Entrez Gene (20130726): GeneID:27347 -> Molecular function: GO:0004702 [receptor signaling protein serine/threonine kinase activity] evidence: NAS GeneID:27347 -> Molecular function: GO:0005524 [ATP binding] evidence: NAS GeneID:27347 -> Molecular function: GO:0019901 [protein kinase binding] evidence: IEA GeneID:27347 -> Biological process: GO:0006468 [protein phosphorylation] evidence: NAS GeneID:27347 -> Biological process: GO:0006950 [response to stress] evidence: NAS GeneID:27347 -> Biological process: GO:0023014 [signal transduction by phosphorylation] evidence: NAS GeneID:27347 -> Biological process: GO:0043268 [positive regulation of potassium ion transport] evidence: IEA GeneID:27347 -> Biological process: GO:0050727 [regulation of inflammatory response] evidence: IEA GeneID:27347 -> Cellular component: GO:0005634 [nucleus] evidence: NAS GeneID:27347 -> Cellular component: GO:0005737 [cytoplasm] evidence: NAS GeneID:27347 -> Cellular component: GO:0005856 [cytoskeleton] evidence: IEA GeneID:27347 -> Cellular component: GO:0016323 [basolateral plasma membrane] evidence: IEA GeneID:27347 -> Cellular component: GO:0016324 [apical plasma membrane] evidence: IEA ANNOTATIONS from NCBI Entrez Gene (20130726): NP_037365 -> EC 2.7.11.1
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