LOCUS       XM_039093840            1001 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus mast cell protease 8 (Mcpt8),
            transcript variant X1, mRNA.
ACCESSION   XM_039093840
VERSION     XM_039093840.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051350.1) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /sex="male"
                     /tissue_type="kidney"
                     /country="USA: Wisconsin, Milwaukee, Medical College of
                     Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..1001
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="mast cell protease 8; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 2
                     mRNAs, 3 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 29 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:29269"
                     /db_xref="RGD:3067"
     CDS             103..912
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /codon_start=1
                     /product="mast cell protease 8 isoform X1"
                     /protein_id="XP_038949768.1"
                     /db_xref="GeneID:29269"
                     /db_xref="RGD:3067"
                     /translation="

MFLFLFFLVAILPVNTEGGEIIWGTESKPHSRPYMAFIKFYDSNSEPHHCGGFLVAKDIVMTAAHCNGRNIKVTLGAHNIKKQENTQVISVVKAKPHENYDRDSHFNDIMLLKLERKAQLNGVVKTIALPRSQDWVKPGQVCTVAGWGRLANCTSSNTLQEVNLEVQKGQKCQDMSEDYNDSIQLCVGNPSEGKATGKENGRAKSSWPEGRTQPSRDTLGDSGGPFVCDGVAQGIVSYRLCTGTLPRVFTRISSFIPWIQKTMKVLQQS"

     misc_feature    163..888
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...; Region:
                     Tryp_SPc; cd00190"
                     /db_xref="CDD:238113"
     misc_feature    163..165
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     misc_feature    order(295..297,424..426,766..768)
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="active site"
                     /db_xref="CDD:238113"
     misc_feature    order(685..687,811..813,817..819)
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="substrate binding sites [chemical binding]; other
                     site"
                     /db_xref="CDD:238113"
ORIGIN      

ataagagatctctggctctgcgcctctttcctcacctgatgttttaaaagttgtgagaagtaacgaagtgaccagttgagacctggtcagaccctgaagaggatgttcctgttcctgttcttcctggtggccatcctaccagtcaacactgaaggaggagagatcatatggggtacagagtccaaaccccactcccggccctacatggcattcataaagttttatgatagtaattcagaaccccatcactgtggcggtttcctggtggcaaaagacatcgtaatgacagcagctcactgtaatggaagaaatataaaagtaaccttaggtgctcacaatatcaagaaacaagaaaacacccaggttatctctgttgtaaaagccaaacctcacgagaactatgacagagattcacattttaatgacatcatgctcctgaagttggaacgcaaagctcaactcaatggtgttgtgaagactattgcccttcctaggagccaggactgggtgaaacctgggcaggtgtgcacagtggcaggttggggacgcttggccaattgtacttcgtctaacacacttcaagaagtgaatctagaagttcagaaaggccagaagtgccaagacatgtccgaagactacaacgactccatccagctttgtgtgggaaaccccagcgaggggaaggctactggtaaggaaaatggaagagctaagtctagttggcctgaaggaaggacacagccaagcagagacacactaggagactcagggggtccctttgtgtgcgatggagtggcccagggcattgtcagttatcgcttgtgtactgggacacttcctcgagtattcaccagaatctccagctttataccgtggattcagaaaacaatgaaagtccttcaacaatcctagaacacaaaacctgtgtctgggccaatgtccagcatcctggggtatggctatctgagtcttaataaagaaatctgtctgcaggaaagaga

//