LOCUS       XM_017595084           14897 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus plectin (Plec), transcript variant X4,
            mRNA.
ACCESSION   XM_017595084
VERSION     XM_017595084.2
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051342.1) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Jan 21, 2021 this sequence version replaced XM_017595084.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /sex="male"
                     /tissue_type="kidney"
                     /country="USA: Wisconsin, Milwaukee, Medical College of
                     Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..14897
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="plectin; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 1 mRNA, 62 ESTs, 42 long
                     SRA reads, 5 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 29 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:64204"
                     /db_xref="RGD:621649"
     CDS             113..13732
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /codon_start=1
                     /product="plectin isoform X4"
                     /protein_id="XP_017450573.2"
                     /db_xref="GeneID:64204"
                     /db_xref="RGD:621649"
                     /translation="

MDPSRAIQHEISSLKDERDRVQKKTFTKWVNKHLIKHWRAEAQRHISDLYEDLRDGHNLISLLEVLSGDSLPRERDVIRSVRLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTTSWRDGRLFNAIIHRHKPMLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPGAQDGVRANELQLRWQEYRELVLLLLQWIRHHTAAFEERKFPSSFEEIEILWCQFLKFKETELPAKEADKNRSKGIYQSLEGAVQAGQLKIPPGYHPLDVEKEWGKLHVAILEREKQLRSEFERLECLQRIVSKLQMEAGLCEEQLNQADSLLQSDIRLLASGKAAQRAGEVERDLDKADGMIRLLFNDVQTLKDGRHPQGEQMYRRVYRLHERLVAIRTEYNLRLKAGVGAPVTQVTLQSTQRRPELEDSTLRYLHDLLAWVEENQRRIDGAEWGVDLPSVEAQLGSHRGMHQSIEEFRAKIERARNDESQLSPATRGAYRDCLGRLDLQYAKLLNSSKARLRSLESLHGFVAAATKELMWLNEKEEEEVGFDWSDRNTNMAAKKESYSALMRELEMKEKKIKEIQNTGDRLLREDHPARPTVESFQAALQTQWSWMLQLCCCIEAHLKENTAYFQFFSDVREAEEQLQKLQETLRRKYSCDRSITVTRLEDLLQDAQDEKEQLNEYKGHLSGLAKRAKAIVQLKPRNPAHPVRGHVPLLAVCDYKQVEVTVHKGDQCQLVGPAQPFHWKVLGGSSSEAAVPSVCFLVPPPNQEAQEAVARLEAQHQALVTLWHQLHVDMKSLLAWQSLNRDIQLIRSWSLVTFRTLKPEEQRQALRNLELHYQAFLRDSQDAGGFGPEDRLVAEREYGSCSRHYQQLLQSLEQGEQEESRCQRCISELKDIRLQLEACETRTVHRLRLPLDKDPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLALPEPSPAAPTLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRSTQGAEEVLKTHEEHLKEAQAVPATLQELEVTKASLKKLRAQAEAQQPVFNTLRDELRGAQEVGERLQQRHGERDVEVERWRERVTQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLSSWLQDAKSRQEQIQAVPIANSQAAREQLRQEKALLEEIERHGEKVEECQKFAKQYINAIKDYELQLITYKAQLEPVASPAKKPKVQSGSESVIQEYVDLRTRYSELTTLTSQYIKFISETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAELEARELQRRMQEEVTRREEAAVDAQQQKRSIQEELQHLRQSSEAEIQAKAQQVEAAERSRMRIEEEIRVVRLQLETTERQRGGAEDELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEAELALRVKAEAEAAREKQRALQALDELKLQAEEAERRLRQAEAERARQVQVALETAQRSAEVELQSKRASFAEKTAQLERTLQEEHVTVTQLREEAERRAQQQAEAERAREEAERELERWQLKANEALRLRLQAEEVAQQKSLAQADAEKQKEEAEREARRRGKAEEQAVRQRELAEQELEKQRQLAEGTAQQRLAAEQELIRLRAETEQGEQQRQLLEEELARLQHEATAATQKRQELEAELAKVRAEMEVLLASKARAEEESRSTSEKSKQRLEAEAGRFRELAEEAARLRALAEEAKRQRQLAEEDAARQRAEAERVLTEKLAAISEATRLKTEAEIALKEKEAENERLRRLAEDEAFQRRRLEEQAAQHKADIEERLAQLRKASESELERQKGLVEDTLRQRRQVEEEIMALKASFEKAAAGKAELELELGRIRSNAEDTMRSKELAEQEAARQRQLAAEEEQRRREAEERVQRSLAAEEEAARQRKVALEEVERLKAKVEEARRLRERAEQESARQLQLAQEAAQKRLQAEEKAHAFVVQQREEELQQTLQQEQNMLERLRSEAEAARRAAEEAEEAREQAEREAAQSRKQVEEAERLKQSAEEQAQAQAQAQAAAEKLRKEAEQEAARRAQAEQAALKQKQAADAEMEKHKKFAEQTLRQKAQVEQELTTLRLQLEETDHQKSILDEELQRLKAEVTEAARQRSQVEEELFSVRVQMEELGKLKARIEAENRALILRDKDNTQRFLEEEAEKMKQVAEEAARLSVAAQEAARLRQLAEEDLAQQRALAEKMLKEKMQAVQEATRLKAEAELLQQQKELAQEQARRLQEDKEQMAQQLVEETQGFQRTLEAERQRQLEMSAEAERLKLRMAEMSRAQARAEEDAQRFRKQAEEIGEKLHRTELATQEKVTLVQTLEIQRQQSDQDAERLREAIAELEREKEKLKQEAKLLQLKSEEMQTVQQEQILQETQALQKSFLSEKDSLLQRERFIEQEKAKLEQLFQDEVAKAKQLQEEQQRQQQQMEQEKQELVASMEEARRRQREAEEGVRRKQEELQRLEQQRQQQEKLLAEENQRLRERLQRLEEEHRAALAHSEEIATSQAAATKALPNGRDALDGPSMEAEPEYTFEGLRQKVPAQQLQEAGILSMEELQRLTQGHTTVAELTQREDVHRYLKGGSSIAGLLLKPTNEKLSVYTALQRQLLSPGTALILLEAQAASGFLLDPVRNRRLTVNEAVKEGVVGPELHHKLLSAERAVTGYKDPYTGEQISLFQAMKKDLIVRDHGIRLLEAQIATGGIIDPVHSHRVPVDVAYQRGYFDEEMNRVLADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLRLLPLTDKAAKGGELVYTDTEARDVFEKATVSAPFGKFQGKTVTIWEIINSEYFTAEQRRDLLRQFRTGRITVEKIIKIVITVVEEHERKGQLCFEGLRALVPAAELLDSGVISHEVYQQLQRGERSVREVAEADEVRQALRGTSVIAGVWLEEAGQKLSIYEALRRDLLQPEVAVALLEAQAGTGHIIDPATSARLTVDEAVRAGLVGPEMHEKLLSAEKAVTGYRDPYSGQSVSLFQALKKGLIPREQGLRLLDAQLSTGGIVDPSKSHRVPLDVAYARGYLDKETNRALTSPRDDARVYLDPSTREPVTYSQLQQRCRSDQLTGLSLLPLSEKAVRARQEEVYSELQARETLEKAKVEVPVGGFKGRALTVWELISSEYFTEEQRQELLRQFRTGKVTVEKVIKILITIVEEVETQRQERLSFSGLRAPVPASELLASKILSRTQFEQLKDGKTSVKDLSEVGSVRTLLQGSGCLAGIYLEDSKEKVTIYEAMRRGLLRPSTATLLLEAQAATGFLVDPVRNQRLYVHEAVKAGVVGPELHEKLLSAEKAVTGYKDPYSGSTISLFQAMKKGLVLRDHAIRLLEAQIATGGIIDPVHSHRLPVDVAYQRGYFDEEMNRVLADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLRLLPLRGAEKTEVVETTQVYTEEETRRAFEETQIDIPGGGSHGGSSMSLWEVMQSDMIPEDQRARLMADFQAGRVTKERMIIIIIEIIEKTEIIRQQNLASYDYVRRRLTAEDLYEARIISLETYNLFREGTKSLREVLEMESAWRYLYGTGSVAGVYLPGSRQTLTIYQALKKGLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVGPELHDRLLSAERAVTGYRDPYTEQPISLFQAMKKELIPAEEALRLLDAQLATGGIVDPRLGFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLKRCRRDDNSGQMLLPLSDARKLTFRGLRKQITVEELVRSQVMDEATALQLQEGLTSIEEVTKNLQKFLEGTSCIAGVFVDATKERLSVYQAMKKGIIRPGTAFELLEAQAATGYVIDPIKGLKLTVEEAVRMGIVGPEFKDKLLSAERAVTGYKDPYSGKLISLFQAMKKGLILKDHGIRLLEAQIATGGIIDPEESHRLPVEVAYKRGLFDEEMNEILTDPSDDTKGFFDPNTEENLTYLQLMERCITDPQTGLCLLPLKEKKRERKTSSKSSVRKRRVVIVDPETGKEMSVYEAYRKGLIDHQTYLELSEQECEWEEITISSSDGVVKSMIIDRRSGRQYDIDDAITKNLIDRSALDQYRAGTLSITEFADMLSGNAGGFRSRSSSVGSSSSYPISSAVPRTQLASWSDPTEETGPVAGILDTETLEKVSITEAMHRNLVDNITGQRLLEAQACTGGIIDPSTGERFPVTEAVNKGLVDKIMVDRINLAQKAFCGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVSLDEALQRGTVDARTAQKLRDVSAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEAAAQSSKGYYSPYSVSGSGSTAGSRTGSRTGSRAGSRRGSFDATGSGFSMTFSSSSYSSSGYGRRYASGPSASLGGPESAVA"

     misc_feature    167..532
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="first calponin homology (CH) domain found in the
                     plectin/dystonin/MACF1 family; Region: CH_PLEC-like_rpt1;
                     cd21188"
                     /db_xref="CDD:409037"
     misc_feature    order(176..178,188..190,389..391,395..400,407..412,
                     416..418,443..469,482..484,488..493,497..502,509..514,
                     521..523)
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="putative actin binding site [polypeptide binding];
                     other site"
                     /db_xref="CDD:409037"
     misc_feature    569..886
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="second calponin homology (CH) domain found in
                     plectin and similar proteins; Region: CH_PLEC_rpt2;
                     cd21238"
                     /db_xref="CDD:409087"
     misc_feature    order(575..577,587..589,737..739,743..748,755..760,
                     764..766,791..817,836..838,842..847,851..856,863..868,
                     875..877)
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="putative actin binding site [polypeptide binding];
                     other site"
                     /db_xref="CDD:409087"
     misc_feature    order(659..661,668..670,728..739,743..748)
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:409087"
     misc_feature    1940..2476
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; Region: SPEC;
                     cd00176"
                     /db_xref="CDD:238103"
     misc_feature    2249..2266
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     misc_feature    2465..2665
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="SH3 domain; Region: SH3_10; pfam17902"
                     /db_xref="CDD:407754"
     misc_feature    2768..3001
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Spectrin like domain; Region: Spectrin_like;
                     pfam18373"
                     /db_xref="CDD:436447"
     misc_feature    <3164..4774
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="DNA repair exonuclease SbcCD ATPase subunit
                     [Replication, recombination and repair]; Region: SbcC;
                     COG0419"
                     /db_xref="CDD:223496"
     misc_feature    <4121..6241
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="MAEBL; Provisional; Region: PTZ00121"
                     /db_xref="CDD:173412"
     misc_feature    <5252..7795
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="MAEBL; Provisional; Region: PTZ00121"
                     /db_xref="CDD:173412"
     misc_feature    8159..8275
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    8273..8389
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    8384..8494
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    8501..8617
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    9137..9250
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    9257..9373
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    9368..9478
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    9485..9601
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    10136..10252
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    10250..10366
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    10361..10456
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    10478..10594
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    11138..11248
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    11255..11371
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    11366..11476
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    11483..11599
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    11864..11977
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    11984..12100
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    12212..12328
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    <12515..12601
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    12911..13012
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: PLEC; smart00250"
                     /db_xref="CDD:197605"
     misc_feature    13019..13135
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
     misc_feature    13247..13363
                     /gene="Plec"
                     /gene_synonym="Pcn; Plec1; Pltn"
                     /note="Plectin repeat; Region: Plectin; pfam00681"
                     /db_xref="CDD:425816"
ORIGIN      

cccacacctcccagctcctccttcaggctgcagtggtggccttgggcagtgcggtgctgtgcagtcctgggcacggactgcagcacaccgttctcagcccctaagtgaagacatggacccctcgagagccatccagcatgaaatcagctccctcaaagatgaacgagaccgtgtgcagaagaaaactttcaccaagtgggtcaataaacaccttatcaagcactggagggcagaggctcaaaggcacatcagtgacctgtatgaagacctccgtgatggccacaacctcatctccctgctggaagtcctctcaggagacagcctgccgagggagcgggacgtaatcaggagtgtacgcctgccccgagagaaggggaggatgcgtttccacaagctgcaaaacgttcagattgccctggactatctccgacatcggcaggtgaagttggtgaacatcagaaatgatgacatcgccgatggcaaccccaagctgaccctgggcctgatctggacaatcatcctgcacttccagatctcagacattcaggtgagcggccagtccgaggacatgacagccaaggagaagctgctgctgtggtctcagcgtatggtagagggctaccaaggcctgcgctgtgacaacttcaccaccagctggcgcgacggccggctcttcaatgctatcatccacaggcacaagcccatgctcatagacatgaataaagtgtaccgacagaccaacctggagaacctagaccaggccttctccgtggcagagcgggacctgggagttaccaggctcctggacccagaagatgtggatgtccctcagcctgatgagaagtccatcatcacctacgtttcatccctgtatgatgccatgccccgtgtgccgggcgcacaggatggagtgagggccaatgagctgcagcttcgttggcaagagtaccgggagcttgtgctgctgctgctacagtggatccggcaccacaccgctgcttttgaggagcgcaagttcccctccagctttgaggagattgagatcctatggtgccagtttctgaagttcaaggagacagaacttcctgccaaggaggcagacaagaaccggtccaaaggcatctaccagtctttggagggagcagtacaagcaggccagctcaagattccccctggctaccacccgctagacgtggaaaaggagtggggcaagctgcacgtggccatcctggagcgggagaagcaactccggagcgagtttgagaggctggagtgtcttcagcgcattgtgagcaagctacagatggaggctgggctatgtgaggagcagctgaaccaggccgattccctactgcagtcggatattcggctgctggcctcaggcaaggcggcacagcgggctggggaagtggagagagacctggacaaggctgatggtatgatccggctgttgttcaatgatgtgcagacccttaaagatgggcggcatccacagggtgaacagatgtaccggagggtgtatcgtctgcatgagcgcctggtagccatccgcactgaatacaacctccggctgaaggcaggggtgggtgcccctgtgacccaggtgaccctgcagagcacacagaggcgcccagagctagaggactccacactgcgttacctgcacgacctgctggcatgggtggaggagaaccagcgtcgaatagacggtgctgagtggggcgtggacttgcccagtgtagaggcacagctgggcagccaccgaggcatgcatcagtctattgaggaattccgggccaagatcgagcgggctcggaatgatgagagccagctctcccctgccacccgaggtgcctaccgagactgcctgggccgcctggacctgcagtatgcaaagctgctgaactcctccaaggcccgcctccggtccctggagagcttgcatgggtttgtggcggcagctaccaaggagctgatgtggctgaatgagaaggaagaggaagaagtgggctttgattggagtgaccgcaacaccaacatggctgccaagaaagaaagttactcggccctgatgcgtgagctggagatgaaggaaaagaaaattaaggagatccagaacacgggggacaggttgctgcgggaagaccatcctgcccggcccacagtggagtccttccaggctgccctgcagacacagtggagctggatgctgcagctgtgttgctgcattgaagcgcacttgaaagagaacacagcctacttccagttcttctcagatgttcgggaggctgaggaacagttgcagaaactacaggagacgttacgcaggaagtacagctgtgaccgctccatcactgtcacaaggcttgaggacctgctgcaggatgcccaggatgagaaggagcaactgaatgagtacaaagggcacctctcaggcctggccaagcgggccaaggctattgtgcagctgaagccacgcaaccctgcccaccctgtgcggggtcacgtgcccctgctagctgtgtgtgactacaagcaggtggaggtgactgtgcacaagggtgaccaatgccagctggtgggccctgcccagccgttccactggaaggtgctcggtggttccagcagtgaggctgccgtgccttctgtgtgctttcttgtgccgccacccaaccaggaggcccaggaagctgttgctaggctggaggcccagcatcaggccctggttactctgtggcaccagcttcacgtggacatgaagagtcttctggcatggcagagcctcaatcgtgacatacagctcatccggtcctggtccctagtcacgttccgcacgctgaagcccgaggagcagcggcaagctctgcgcaacctggagttgcactaccaggccttccttcgagacagccaggacgctggtggctttgggcccgaggaccggctggtggcagagcgcgaatatggatcttgtagtcgccactaccagcagctgctacaaagcctggagcagggtgagcaggaggagtctcgctgtcagcgatgcatctcggagctcaaggacattcggctgcaactggaggcctgtgagactcggactgtgcaccgtctgcggctgccactggataaagaccccgcacgggagtgtgcccagcgcatcgctgagcaacagaaagcacaggctgaggtggaggggctgggcaagggagttgcccggctgtctgctgaggctgagaaagttctggccttgccagagccgtcacctgctgcaccaactctgcgctcggagttggaattgaccctgggcaagctggaacaggtcagaagcctgtctgccatctacttggagaaactcaagaccatcagcttggtaattcgcagtacccagggggctgaggaggtgcttaaaacacacgaggagcacctgaaggaggcccaggccgtgcctgccacactccaagagctcgaagtcaccaaggcttcactaaagaagctgcgggcccaggcggaggcacagcagcctgtattcaacaccctacgagatgagctgaggggggcacaggaagttggtgaacggctacagcagcggcatggtgagcgggacgtggaagtagagcgctggcgagaacgtgtcactcagttgctggagcgctggcaggctgtgctagcccagactgatgtgcggcagcgggagcttgaacagctgggccgccaacttcgctactaccgtgaaagtgcggatccgctgagctcctggctgcaggatgccaagagccggcaagagcagatccaggctgtgccaatagccaacagtcaggctgcacgagaacagctgcgccaggagaaggccctgctggaggagattgagcgccatggtgagaaggttgaggagtgccagaagtttgctaagcagtacatcaatgcaatcaaggactatgagctccagctgatcacctacaaggctcagcttgaacctgtggcctcccccgccaagaagcccaaggttcagtctggatcggagagcgtcatccaggagtacgtggatctgcgtacacgctacagtgagctgaccacactcacgagtcagtacatcaagttcatcagtgagacactgcgccgcatggaagaggaagagcggctggctgagcaacagcgggcagaggagcgggagcgcctggccgaggtggaggccgcgctggagaagcagcggcagctggctgaggcccatgcccaggccaaggcacaggccgagctggaggcacgagaactgcagcggcgcatgcaggaggaggtgacgcggcgcgaggaggcggcggtggacgcacagcaacagaagcgcagcatccaagaggagctgcagcatctgcggcaaagctcagaggcagagatccaggccaaggcccagcaggtggaggctgcagagcgcagccgcatgcgcattgaggaagagatccgcgtagtccgtctgcagctagagacaactgagcgtcagcgtggaggggcggaggatgagctgcaggctctgcgtgcacgggctgaggaggcagaagcacagaagcggcaggctcaggaggaagccgagcgcttgcggaggcaggtgcaggatgagagccaacgcaaacggcaggcggaggccgagctggccctgcgtgtgaaggcagaagcggaggcagcgcgagagaagcagcgggccctgcaggctctggatgaactgaaactgcaggccgaggaggccgaacggcggctgcgccaagccgaggcagagagggctcgccaagtgcaggtagccctggagacagcgcagcgtagtgcagaagtggagctgcagagcaagcgtgcgtcctttgcagagaagaccgcacagttggagcgcacgctgcaggaagagcacgtgacagtgacacagctgcgggaggaggcggaacggcgggcacagcagcaggctgaagccgagcgagcccgtgaggaagccgagcgggagctggagcgctggcagctgaaggccaatgaggcgctgcggctgcggctgcaggcagaggaggtggcacagcagaagagcctggcccaggccgatgcagagaagcagaaggaagaggcagaacgggaagcccggcggcggggcaaggcagaggagcaggccgtgcggcagcgagagctggctgagcaggagctggagaagcagcggcagctggcagagggcaccgcccagcagcgcctggctgccgagcaggagctgattcgcctgcgggcagagacggagcaaggtgagcagcagcggcagctgctggaggaagagctggcccggctacagcacgaagcgacagcagccacacagaagcgccaggagctggaggctgagctggcgaaggttcgggcagagatggaggtactgctggccagcaaggcacgagccgaagaggagtctcgctccaccagtgaaaagtccaagcagaggctggaagctgaggcagggcggtttcgagagctggctgaggaggctgcccgcctgcgtgctctggccgaggaggccaagcggcagcggcagttggccgaggaggacgcggcacgccagcgggccgaggcggagcgagtgcttacggagaagctggctgccatcagtgaggccacaaggctcaagacggaggcagagattgcactcaaagagaaggaggccgagaacgagcgcctgaggcgcctggctgaagatgaggccttccagcggcgccggctggaggagcaggcagcacagcacaaggcagacatagaggagcgcctggcccagctgcgcaaggcatccgagagcgagctggagcgacagaaggggttggtggaggataccctgcggcagcggcggcaggtggaggaggagatcatggctctgaaggcgagcttcgagaaggccgcggctggcaaggcagaactggagctggagcttggccgcatccgcagcaatgccgaggacaccatgcgcagcaaggagctggccgagcaggaggcagcgcggcagcggcagttggcagctgaggaggagcagaggcgccgggaagccgaggagcgggtgcagaggagcctggcagcggaggaggaagccgcacggcagcgcaaggtcgcactggaggaagtcgagcggctcaaggccaaggttgaggaagcgcggcgcctgcgagagcgagctgagcaggagtctgcgaggcagctgcagctggcccaggaggctgcccagaaacggctgcaggcggaggagaaggcgcacgcctttgtggtgcagcagcgagaagaggagctgcagcagactcttcagcaagagcagaacatgctggagcggctgcggagcgaggcagaggcagcgcggcgagctgctgaggaggcggaggaggcccgggagcaggcagaacgtgaggcagcgcagtctaggaagcaagtggaagaggccgagcggctgaagcagtcggcagaggagcaggctcaggcccaggcccaggcgcaggcggctgcagagaaactgcgcaaggaagcggagcaggaggcggcgcgtcgggcccaggcggagcaggctgcgttgaaacagaagcaggcagccgacgcggagatggagaagcacaagaagtttgcagagcagacgctacggcagaaggctcaggtagagcaggagctgaccacgctgaggctgcagctcgaggagaccgaccaccagaagagcatcctggatgaggagctgcagcggctaaaggctgaggtaacagaggcagcccggcagcgtagccaggtagaggaggagctcttctctgtccgcgtgcagatggaggagctgggcaaactcaaggctcgcattgaagctgaaaaccgggcactcatccttcgtgacaaggacaacacacagcgcttcctggaggaggaggccgagaagatgaaacaggtggcagaggaagctgcacggttgagcgtagctgcccaggaggcagcaaggctgcggcagctagccgaggaggacctggcccagcagcgggccctggcggagaagatgctgaaggagaagatgcaggcggtgcaggaagccacaaggctcaaggctgaggctgagctgctgcagcagcagaaggagctggcacaggagcaggcccggcggctgcaggaggacaaggagcaaatggctcagcagttggtagaggagacacagggtttccagcggaccctggaggctgagcggcagcggcagctagaaatgagcgcagaggctgaacgcctcaagttgcgcatggctgagatgagccgggctcaggcccgtgcagaggaggatgcccagcgcttccggaagcaggctgaagagatcggcgaaaagctgcaccgcactgaactcgctacacaggagaaggtgacattggtgcagactctcgagatccagcgacagcagagtgaccaagatgccgagcgtctgagggaggccattgctgagctggagcgtgagaaggagaagctcaagcaggaggcgaagttactgcagctcaagtctgaggagatgcagactgtgcagcaggagcagatactgcaggagacacaggccctgcagaagagctttctctctgagaaggacagcttgctgcaacgcgaacgcttcatcgagcaggagaaggccaagctggagcagcttttccaggacgaggtggcaaaagcaaaacagctgcaggaggagcagcagcggcagcagcagcagatggagcaggaaaagcaggagctggtggccagcatggaggaggcccggaggcggcagcgtgaggcagaggagggtgtgaggcgcaagcaagaggaactgcagcgtctggagcagcagcggcagcagcaggagaaactactggcagaggagaaccagaggctgcgggagcggctgcagcgcctggaggaagagcaccgagctgcgttggcgcactctgaggagatcgccacctcccaggctgctgccacaaaagcactgcccaatggccgcgacgcacttgatggcccctccatggaggccgagcccgagtacaccttcgagggattacgtcagaaggtgccagctcagcagctacaggaagcaggcattctgagcatggaggaactgcagcgtttgacacagggtcacaccacggtggctgagctcacgcagcgggaagatgtgcaccgatacctgaagggcggcagcagcatcgcaggattgctcctgaagcccaccaatgagaaactgagtgtctacacagccctacagcggcagctgctcagccctggaacagcccttatcttgcttgaggcccaggcagcctcgggcttcctgctggaccctgtgcggaaccggcggctgacggtcaatgaggctgtgaaggagggtgtggtgggtcccgagctgcaccacaagctgctgtcagctgagcgtgccgtcactggctacaaggacccttacacaggagaacagatctctctcttccaggccatgaagaaggacctcattgtcagggaccatggcatccgcctgctggaagcccagatcgccacaggtggcatcattgaccctgtacacagccaccgtgttcccgtggacgtggcctaccagcgtggctacttcgatgaggagatgaaccgtgtgctggctgacccaagcgatgacaccaagggcttctttgaccccaacactcacgagaacctcacgtacctgcagctgctggagcgctgtgtggaggaccccgagacaggcctgcgcctcctgccactcacagacaaggctgccaagggtggtgagctggtgtacactgacacggaggcccgtgacgtcttcgaaaaggccacagtgtctgcaccattcggcaagttccagggcaagaccgtgaccatctgggagatcatcaactcagagtacttcacagcggagcagcgacgggacctgctccggcagttccgcacgggccgcatcacggtggagaagatcatcaagattgtcatcacggtggtagaggaacacgagcggaagggccagctctgctttgagggcctccgtgcccttgtgcctgctgcagagctgctggacagtggagtcatcagtcatgaagtctaccagcagctgcagcggggtgagcgctctgtgcgggaagtggccgaggcagacgaggtgaggcaggccctgcggggtaccagtgtcattgccggtgtgtggctggaagaagcagggcagaagctgagcatctatgaggccctgaggagagatttgctgcagccagaggtggctgtggccttgctggaggcccaggctggcactgggcacatcattgaccctgccacgagtgccaggctgactgtggatgaggcagtgcgtgctggcctggtgggtcctgagatgcacgagaagctcttgtcagctgagaaggctgtaacaggctatagggatccctactcgggacagagcgtctcgctcttccaggctctgaagaagggtctcatcccccgagaacagggcctgcgcctgctggatgcccagttatccactggtggcattgtagaccccagcaaaagccaccgtgtgcccctggatgttgcctatgcccggggctacctggacaaagagactaacagggccctgacgtcacccagagacgatgccagagtctaccttgaccccagcacccgggagccagtcacctacagccagctccaacagcggtgccggtctgaccagctgactgggttgagcctactgcccctctcagagaaggccgtccgggcccggcaggaagaggtctactctgagctccaggcccgtgagacattggagaaggccaaggtggaggttcctgtgggcggctttaagggcagggcgctgacagtgtgggagctcataagctcggaatacttcactgaggagcagcggcaggagctgctacggcagttccgcacaggcaaggtcactgtagagaaggtcatcaagattcttatcaccattgtggaggaggtggagactcaacggcaggagagactgtcc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