LOCUS       NM_031025               2628 bp    mRNA    linear   ROD 12-NOV-2023
DEFINITION  Rattus norvegicus dihydrolipoamide S-acetyltransferase (Dlat),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_031025 XM_343389
VERSION     NM_031025.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R,
            Rowland EA, Kang Y, Shenk T and Cristea IM.
  TITLE     Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase
            complex activity
  JOURNAL   Cell 159 (7), 1615-1625 (2014)
   PUBMED   25525879
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Zhao X, Leon IR, Bak S, Mogensen M, Wrzesinski K, Hojlund K and
            Jensen ON.
  TITLE     Phosphoproteome analysis of functional mitochondria isolated from
            resting human muscle reveals extensive phosphorylation of inner
            membrane protein complexes and enzymes
  JOURNAL   Mol Cell Proteomics 10 (1), M110.000299 (2011)
   PUBMED   20833797
REFERENCE   3  (bases 1 to 2628)
  AUTHORS   Yu X, Hiromasa Y, Tsen H, Stoops JK, Roche TE and Zhou ZH.
  TITLE     Structures of the human pyruvate dehydrogenase complex cores: a
            highly conserved catalytic center with flexible N-terminal domains
  JOURNAL   Structure 16 (1), 104-114 (2008)
   PUBMED   18184588
REFERENCE   4  (bases 1 to 2628)
  AUTHORS   Lengyel JS, Stott KM, Wu X, Brooks BR, Balbo A, Schuck P, Perham
            RN, Subramaniam S and Milne JL.
  TITLE     Extended polypeptide linkers establish the spatial architecture of
            a pyruvate dehydrogenase multienzyme complex
  JOURNAL   Structure 16 (1), 93-103 (2008)
   PUBMED   18184587
REFERENCE   5  (bases 1 to 2628)
  AUTHORS   Werner HB, Kuhlmann K, Shen S, Uecker M, Schardt A, Dimova K,
            Orfaniotou F, Dhaunchak A, Brinkmann BG, Mobius W, Guarente L,
            Casaccia-Bonnefil P, Jahn O and Nave KA.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   6  (bases 1 to 2628)
  AUTHORS   Yang D, Song J, Wagenknecht T and Roche TE.
  TITLE     Assembly and full functionality of recombinantly expressed
            dihydrolipoyl acetyltransferase component of the human pyruvate
            dehydrogenase complex
  JOURNAL   J Biol Chem 272 (10), 6361-6369 (1997)
   PUBMED   9045657
REFERENCE   7  (bases 1 to 2628)
  AUTHORS   Maury J, Kerbey AL, Priestman DA, Patel MS, Girard J and Ferre P.
  TITLE     Pretranslational regulation of pyruvate dehydrogenase complex
            subunits in white adipose tissue during the suckling-weaning
            transition in the rat
  JOURNAL   Biochem J 311 (Pt 2) (Pt 2), 531-535 (1995)
   PUBMED   7487891
REFERENCE   8  (bases 1 to 2628)
  AUTHORS   Christen U, Jeno P and Gut J.
  TITLE     Halothane metabolism: the dihydrolipoamide acetyltransferase
            subunit of the pyruvate dehydrogenase complex molecularly mimics
            trifluoroacetyl-protein adducts
  JOURNAL   Biochemistry 32 (6), 1492-1499 (1993)
   PUBMED   8431428
REFERENCE   9  (bases 1 to 2628)
  AUTHORS   Matuda S, Nakano K, Ohta S, Shimura M, Yamanaka T, Nakagawa S,
            Titani K and Miyata T.
  TITLE     Molecular cloning of dihydrolipoamide acetyltransferase of the rat
            pyruvate dehydrogenase complex: sequence comparison and
            evolutionary relationship to other dihydrolipoamide
            acyltransferases
  JOURNAL   Biochim Biophys Acta 1131 (1), 114-118 (1992)
   PUBMED   1581353
REFERENCE   10 (bases 1 to 2628)
  AUTHORS   Gershwin,M.E., Mackay,I.R., Sturgess,A. and Coppel,R.L.
  TITLE     Identification and specificity of a cDNA encoding the 70 kd
            mitochondrial antigen recognized in primary biliary cirrhosis
  JOURNAL   J Immunol 138 (10), 3525-3531 (1987)
   PUBMED   3571977
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC107440.1.
            
            On Nov 1, 2005 this sequence version replaced XM_343389.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC107440.1, SRR8487226.85710.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q23"
     gene            1..2628
                     /gene="Dlat"
                     /note="dihydrolipoamide S-acetyltransferase"
                     /db_xref="GeneID:81654"
                     /db_xref="RGD:619859"
     exon            1..262
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1909
                     /gene="Dlat"
                     /EC_number="2.3.1.12"
                     /note="E2; PBC; PDC-E2; 70 kDa mitochondrial autoantigen
                     of primary biliary cirrhosis; dihydrolipoamide
                     acetyltransferase component of pyruvate dehydrogenase
                     complex; PDCE2; pyruvate dehydrogenase complex component
                     E2; dihydrolipoamide S-acetyltransferase (E2 component of
                     pyruvate dehydrogenase complex)"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_112287.1"
                     /db_xref="GeneID:81654"
                     /db_xref="RGD:619859"
                     /translation="

MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSYGVRSLCGWSYGSATVPRNRILQQLLGSPSRRSYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTMLL"

     transit_peptide 11..241
                     /gene="Dlat"
                     /note="Mitochondrion.
                     /evidence=ECO:0000269|PubMed:1581353; propagated from
                     UniProtKB/Swiss-Prot (P08461.3)"
     mat_peptide     242..1906
                     /gene="Dlat"
                     /product="Dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex,
                     mitochondrial. /id=PRO_0000162298"
                     /note="propagated from UniProtKB/Swiss-Prot (P08461.3)"
     misc_feature    269..>508
                     /gene="Dlat"
                     /note="pyruvate dehydrogenase subunit beta; Provisional;
                     Region: PRK11892"
                     /db_xref="CDD:237011"
     misc_feature    281..283
                     /gene="Dlat"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P10515; propagated from
                     UniProtKB/Swiss-Prot (P08461.3); phosphorylation site"
     misc_feature    641..1906
                     /gene="Dlat"
                     /note="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase, long form; Region: PDHac_trf_mito;
                     TIGR01349"
                     /db_xref="CDD:273567"
     misc_feature    1361..1363
                     /gene="Dlat"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P10515; propagated from
                     UniProtKB/Swiss-Prot (P08461.3); acetylation site"
     misc_feature    1376..1906
                     /gene="Dlat"
                     /note="propagated from UniProtKB/Swiss-Prot (P08461.3);
                     Region: Catalytic. /evidence=ECO:0000250"
     exon            263..364
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            365..489
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            490..640
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            641..767
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            768..955
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1097
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1165
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1255
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1363
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1479
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1642
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1779
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..2602
                     /gene="Dlat"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

cggtggcagtatgtggcgcgtctgtgcgcggagggtgcagagtgctgttcccagggccgggttcagggctcggtgggcaactttgaagggcccgcggaccggccccgccgcagttcgttgcggcagtgggatacccagctatggggtccgctcgttgtgcggctggagctacggctctgccaccgtcccacgtaaccgcatactgcaacaactcctggggtcgcccagccgccgctcttatagcctccctccgcaccagaaggttccactaccttcactttcccctacaatgcaggcaggcaccatagcccgctgggaaaaaaaagaaggggaaaaaatcagtgagggtgatctaattgcagaggttgaaactgataaagccactgttggatttgagagcttggaggagtgctatatggcaaagatccttgttcctgaggggaccagagatgttccagtaggatctatcatttgtatcacagttgaaaagcctcaggatattgaggcctttaaaaattatacattggattcagcaacagctgccacacaggcagccccagccccagctgcagctccagctgctgccccagctgcaccttctgcaagtgctccaggtagctcctatcccgttcacatgcagattgttcttcctgccctctccccaaccatgaccatgggcaccgtccagaggtgggaaaagaaagtgggagagaagctgagtgaaggagacttgctggcagagatagagaccgacaaggccaccataggctttgaagtacaagaagaaggttatctggcaaaaatcctggtccctgaaggcacaagggatgttcctctgggaaccccgctatgtatcatagtagaaaaacaggaagatatagcagcatttgcagactacaggccaacagaagtgaccagcttaaagccacaggcaccaccacctgtcccacccccagtggcagctgttcctcccatcccccagcctttagcacctaccccctcagccgctcctgctggaccaaagggaagggtgttcgttagccctcttgcaaagaaattggcagcagagaaagggattgacctcacccaagttaaagggacgggaccagaaggcagaatcatcaagaaggacattgactcttttgtgcctactaaggctgctcctgccgctgcagcagctgctcccccgggtccaagagtggcaccaactcctgcaggtgtcttcatagacatccccatcagcaacattcgtcgagtgattgcgcagaggctcatgcagtcgaagcagactatacctcattattacctttctgttgatgtaaatatgggagaggtgctgttggtgcggaaggaacttaataagatgcttgaaggtaaaggaaaaatctccgtcaatgacttcatcataaaagcttcagctttggcctgcctgaaagttcctgaagcaaactcatcttggatggacacagttatacgacaaaatcatgtggttgatgtcagcgttgctgtcagtacccctgcaggacttatcacccctattgtgtttaatgcacacataaaaggactggaaaccattgctagtgatgttgtttctttagcctccaaagcaagagagggtaaacttcagcctcacgagttccagggtgggacatttacaatctccaacttagggatgttcggaattaagaatttctctgcgattattaacccacctcaggcatgtattttggcaattggtgcttccgaggataaactgatccctgcagataatgagaaaggctttgacgtggctagtgtgatgtctgtcacactcagctgtgatcatcgagttgtggatggagcagttggagcccagtggcttgctgaattcaaaaagtaccttgaaaagcctgtaactatgttattataatttccctgcgaacttctattcttcctctgctgacattatatttatatgttattaaacaggtggttcttttaaagtttcactagttaattttattattgagtcagtccagataaattatttgtaatagacataaatgaatcttttgtaaatgccagttacacccaaatgttttacccctttaataattacacatcggtttaaagctgtgtactcctagtgaagggacatgtggcctaactctttggtgagaagaccttcctgggagtatagaatgggcaggattgtggcttcctgcagacagactcataaaagtaacctggatgagatcttggagtttagatttaattgattcaacttatttaagtgtgagggaaacagtcgtgaaaattacttcttatgggaggagtttgaatttacgttggaatctaacagttaaagtctcctgtcacatgaccttagtttatcactcagtaagtgaaacttttaagagtcagcattttcttagacctcttcaaaccaactattatttttatatgaatctctaaataaaatagctttccccacaagataaagatataaaccagcttcttggtataaatgaaattttatgtatttatttataaacaatttcaatgtataacttgagaattctgcagtttcctccaaataaaagaacatgcatattgaaatgaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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