LOCUS       NM_021598                861 bp    mRNA    linear   ROD 17-JUL-2023
DEFINITION  Rattus norvegicus mast cell protease 8 (Mcpt8), mRNA.
ACCESSION   NM_021598 XM_039093841
VERSION     NM_021598.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Puente XS and Lopez-Otin C.
  TITLE     A genomic analysis of rat proteases and protease inhibitors
  JOURNAL   Genome Res 14 (4), 609-622 (2004)
   PUBMED   15060002
REFERENCE   2  (bases 1 to 861)
  AUTHORS   Lutzelschwab C, Pejler G, Aveskogh M and Hellman L.
  TITLE     Secretory granule proteases in rat mast cells. Cloning of 10
            different serine proteases and a carboxypeptidase A from various
            rat mast cell populations
  JOURNAL   J Exp Med 185 (1), 13-29 (1997)
   PUBMED   8996238
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000269.1.
            
            On or before Mar 15, 2021 this sequence version replaced
            XM_039093841.1, NM_021598.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U67911.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                JACYVU010000269.1  1131-1214
            85-235              JACYVU010000269.1  2380-2530
            236-368             JACYVU010000269.1  2855-2987
            369-623             JACYVU010000269.1  3210-3464
            624-861             JACYVU010000269.1  3876-4113
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..861
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="mast cell protease 8"
                     /db_xref="GeneID:29269"
                     /db_xref="RGD:3067"
     exon            1..84
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..776
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="mast cell protease VIII"
                     /codon_start=1
                     /product="mast cell protease 8 precursor"
                     /protein_id="NP_067609.2"
                     /db_xref="GeneID:29269"
                     /db_xref="RGD:3067"
                     /translation="

MFLFLFFLVAILPVNTEGGEIIWGTESKPHSRPYMAFIKFYDSNSEPHHCGGFLVAKDIVMTAAHCNGRNIKVTLGAHNIKKQENTQVISVVKAKPHENYDRDSHFNDIMLLKLERKAQLNGVVKTIALPRSQDWVKPGQVCTVAGWGRLANCTSSNTLQEVNLEVQKGQKCQDMSEDYNDSIQLCVGNPSEGKATGKGDSGGPFVCDGVAQGIVSYRLCTGTLPRVFTRISSFIPWIQKTMKVLQQS"

     sig_peptide     30..83
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     mat_peptide     84..773
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /product="mast cell protease 8"
     misc_feature    90..752
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...; Region:
                     Tryp_SPc; cd00190"
                     /db_xref="CDD:238113"
     misc_feature    90..92
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     misc_feature    order(222..224,351..353,630..632)
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="active site"
                     /db_xref="CDD:238113"
     misc_feature    228..230
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P97594.2); glycosylation site"
     misc_feature    483..485
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P97594.2); glycosylation site"
     misc_feature    567..569
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P97594.2); glycosylation site"
     misc_feature    order(612..614,675..677,681..683)
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /note="substrate binding sites [chemical binding]; other
                     site"
                     /db_xref="CDD:238113"
     exon            85..235
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="alignment:Splign:2.1.0"
     exon            236..368
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="alignment:Splign:2.1.0"
     exon            369..623
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="alignment:Splign:2.1.0"
     exon            624..861
                     /gene="Mcpt8"
                     /gene_synonym="RMCP-10; rMCP-8; rMCP-VIII; RMCP8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agttgagacctggtcagaccctgaagaggatgttcctgttcctgttcttcctggtggccatcctaccagtcaacactgaaggaggagagatcatatggggtacagagtccaaaccccactcccggccctacatggcattcataaagttttatgatagtaattcagaaccccatcactgtggcggtttcctggtggcaaaagacatcgtaatgacagcagctcactgtaatggaagaaatataaaagtaaccttaggtgctcacaatatcaagaaacaagaaaacacccaggttatctctgttgtaaaagccaaacctcacgagaactatgacagagattcacattttaatgacatcatgctcctgaagttggaacgcaaagctcaactcaatggtgttgtgaagactattgcccttcctaggagccaggactgggtgaaacctgggcaggtgtgcacagtggcaggttggggacgcttggccaattgtacttcgtctaacacacttcaagaagtgaatctagaagttcagaaaggccagaagtgccaagacatgtccgaagactacaacgactccatccagctttgtgtgggaaaccccagcgaggggaaggctactggtaagggagactcagggggtccctttgtgtgcgatggagtggcccagggcattgtcagttatcgcttgtgtactgggacacttcctcgagtattcaccagaatctccagctttataccgtggattcagaaaacaatgaaagtccttcaacaatcctagaacacaaaacctgtgtctgggccaatgtccagcatcctggggtatggctatctgagtcttaataaagaaatctgtctgcaggaaa

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