LOCUS       NM_017146                861 bp    mRNA    linear   ROD 23-NOV-2023
DEFINITION  Rattus norvegicus mast cell protease 10 (Mcpt10), mRNA.
ACCESSION   NM_017146 XM_001058119 XM_008770717 XM_017599880 XM_039093843
            XM_341322
VERSION     NM_017146.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Lutzelschwab C, Pejler G, Aveskogh M and Hellman L.
  TITLE     Secretory granule proteases in rat mast cells. Cloning of 10
            different serine proteases and a carboxypeptidase A from various
            rat mast cell populations
  JOURNAL   J Exp Med 185 (1), 13-29 (1997)
   PUBMED   8996238
REFERENCE   2  (bases 1 to 861)
  AUTHORS   Amerik AYu, Yarovoi SV, Grigorenko VG and Antonov VK.
  TITLE     Identification, sequence analysis, and characterization of cDNA
            clones encoding two granzyme-like serine proteinases from rat
            duodenum
  JOURNAL   FEBS Lett 324 (2), 226-230 (1993)
   PUBMED   8508925
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000269.1.
            
            On or before Jul 5, 2022 this sequence version replaced
            XM_039093843.1, NM_017146.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN06680414,
                              SAMN06680417 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                JACYVU010000269.1  120114-120197       c
            85-235              JACYVU010000269.1  118795-118945       c
            236-368             JACYVU010000269.1  118338-118470       c
            369-623             JACYVU010000269.1  117861-118115       c
            624-861             JACYVU010000269.1  117213-117450       c
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..861
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="mast cell protease 10"
                     /db_xref="GeneID:54269"
                     /db_xref="RGD:3063"
     exon            1..84
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..776
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="granzyme-like protein 2; mast cell protease X;
                     granzyme-like protein II"
                     /codon_start=1
                     /product="granzyme-like protein 2 precursor"
                     /protein_id="NP_058842.3"
                     /db_xref="GeneID:54269"
                     /db_xref="RGD:3063"
                     /translation="

MFLFLIFLVAVLPVNTEGGEIVWGTESKPHSRPYMASLMFYYGNSYRHYCGGFLVAKDIVMTAAHCNESNIKVILGAHNIKKRENTQVFSVVKAKPHENYDSHSLFNDIMLLKLERKAQLNGVVKTIALPRSQDWVKPGQVCTVAGWGRLANCTLSNTLQEVNLEVQKGQKCQGMSEDYNDSIQLCVGNPSEGKATGKGDSGGPFVCDGVAQGIVSRRLCTGTLPRVFTRISTFIPWIQKTMKLLQQP"

     sig_peptide     30..83
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     mat_peptide     90..773
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /product="Granzyme-like protein 2. /id=PRO_0000027430"
                     /note="propagated from UniProtKB/Swiss-Prot (Q06606.2)"
     misc_feature    90..752
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...; Region:
                     Tryp_SPc; cd00190"
                     /db_xref="CDD:238113"
     misc_feature    90..92
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     misc_feature    order(222..224,351..353,630..632)
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="active site"
                     /db_xref="CDD:238113"
     misc_feature    483..485
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q06606.2); glycosylation site"
     misc_feature    567..569
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q06606.2); glycosylation site"
     misc_feature    order(612..614,675..677,681..683)
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /note="substrate binding sites [chemical binding]; other
                     site"
                     /db_xref="CDD:238113"
     exon            85..235
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="alignment:Splign:2.1.0"
     exon            236..368
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="alignment:Splign:2.1.0"
     exon            369..623
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="alignment:Splign:2.1.0"
     exon            624..861
                     /gene="Mcpt10"
                     /gene_synonym="GLP II; GLP-2; rMCP-10; rMCP-X"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agttgagacctggtcagaccctgaagaggatgttcctgttcctgatcttcctggtggctgtcctaccagtcaacactgaaggaggagagatcgtatggggtacagagtccaaaccccactcccggccctatatggcatccttaatgttttattatggtaattcatatcgccattactgtggtggtttcctggtggcaaaagacatcgtaatgacagcagctcactgtaatgaaagcaatataaaagtaatcttaggtgctcacaatatcaagaaacgagaaaacacccaggtcttctctgttgtaaaagccaaacctcacgagaactatgacagccattcactgtttaatgacatcatgctcctgaagttggaacgcaaagctcaactcaatggtgttgtgaagactattgcccttcctaggagccaggactgggtgaaacctgggcaggtgtgcacagtggcaggttggggacgcttggccaattgtactttgtctaacacacttcaagaagtgaatctagaagttcagaaaggccagaagtgccaaggcatgtcagaagactacaacgactccatccagctttgtgtgggaaaccccagcgaggggaaggctactggtaagggagactcagggggtccctttgtgtgcgatggagtggcccagggcattgtcagtcgtcgcttgtgtactgggacacttcctcgagtattcaccagaatctccacctttataccgtggattcagaaaacaatgaaactccttcaacaaccctagaacacaaaacctgtgtctgggccaatgtccagcctcctggggtatggctatctaagtcttaataaagaaatctgtctgcaggaaa

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