LOCUS       NM_001007755            2176 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus selenocysteine lyase (Scly), mRNA.
ACCESSION   NM_001007755 XM_343627
VERSION     NM_001007755.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Seale LA, Hashimoto AC, Kurokawa S, Gilman CL, Seyedali A,
            Bellinger FP, Raman AV and Berry MJ.
  TITLE     Disruption of the selenocysteine lyase-mediated selenium recycling
            pathway leads to metabolic syndrome in mice
  JOURNAL   Mol Cell Biol 32 (20), 4141-4154 (2012)
   PUBMED   22890841
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Omi R, Kurokawa S, Mihara H, Hayashi H, Goto M, Miyahara I,
            Kurihara T, Hirotsu K and Esaki N.
  TITLE     Reaction mechanism and molecular basis for selenium/sulfur
            discrimination of selenocysteine lyase
  JOURNAL   J Biol Chem 285 (16), 12133-12139 (2010)
   PUBMED   20164179
  REMARK    GeneRIF: ESI-MS analysis of wild-type and C375A mutant SCL revealed
            that the catalytic reaction proceeds via the formation of an
            enzyme-bound selenopersulfide intermediate on the catalytically
            essential Cys-375 residue.
REFERENCE   3  (bases 1 to 2176)
  AUTHORS   Jafari C, Panzer U, Steinmetz OM, Zahner G, Stahl RA and Harendza
            S.
  TITLE     Enhanced expression of selenocysteine lyase in acute
            glomerulonephritis and its regulation by AP-1
  JOURNAL   Cell Mol Biol Lett 11 (3), 424-437 (2006)
   PUBMED   16874457
  REMARK    GeneRIF: An enhancer element between bp -152 and -298 of the SCL
            5'-regulatory region was identified, with protein binding to an
            AP-1 binding site that may be involved in the regulation of SCL-RNA
            in vivo in an endogenous feedback mechanism.
REFERENCE   4  (bases 1 to 2176)
  AUTHORS   Mihara H, Kurihara T, Watanabe T, Yoshimura T and Esaki N.
  TITLE     cDNA cloning, purification, and characterization of mouse liver
            selenocysteine lyase. Candidate for selenium delivery protein in
            selenoprotein synthesis
  JOURNAL   J Biol Chem 275 (9), 6195-6200 (2000)
   PUBMED   10692412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000215.1.
            
            On Jan 10, 2023 this sequence version replaced NM_001007755.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079358.1, SRR8487227.300561.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               JACYVU010000215.1  24960025-24960127
            104-216             JACYVU010000215.1  24962268-24962380
            217-317             JACYVU010000215.1  24965377-24965477
            318-498             JACYVU010000215.1  24967337-24967517
            499-626             JACYVU010000215.1  24969679-24969806
            627-791             JACYVU010000215.1  24970007-24970171
            792-898             JACYVU010000215.1  24971185-24971291
            899-935             JACYVU010000215.1  24974898-24974934
            936-1019            JACYVU010000215.1  24976505-24976588
            1020-1119           JACYVU010000215.1  24977037-24977136
            1120-1195           JACYVU010000215.1  24978776-24978851
            1196-2176           JACYVU010000215.1  24979658-24980638
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q36"
     gene            1..2176
                     /gene="Scly"
                     /note="selenocysteine lyase"
                     /db_xref="GeneID:363285"
                     /db_xref="RGD:1359514"
     exon            1..103
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1349
                     /gene="Scly"
                     /EC_number="4.4.1.16"
                     /codon_start=1
                     /product="selenocysteine lyase"
                     /protein_id="NP_001007756.1"
                     /db_xref="GeneID:363285"
                     /db_xref="RGD:1359514"
                     /translation="

MDVARNGARGSVESPPNRKVYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQGRTVDQISPEEGTRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDLGVDFLTIVGHKFYGPRIGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERLPNTCNFSIQGSQLRGYMVLAQCQTLLASVGASCHSDHEDRPSPVLLSCGIPVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQLEGPV"

     misc_feature    51..110
                     /gene="Scly"
                     /note="propagated from UniProtKB/Swiss-Prot (Q68FT9.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    51..53
                     /gene="Scly"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q96I15; propagated from
                     UniProtKB/Swiss-Prot (Q68FT9.1); acetylation site"
     misc_feature    102..1334
                     /gene="Scly"
                     /note="Cysteine sulfinate desulfinase/cysteine desulfurase
                     or related enzyme [Amino acid transport and metabolism];
                     Region: NifS; COG1104"
                     /db_xref="CDD:224029"
     misc_feature    399..401
                     /gene="Scly"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96I15; propagated from
                     UniProtKB/Swiss-Prot (Q68FT9.1); phosphorylation site"
     exon            104..216
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            217..317
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            318..498
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            499..626
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            627..791
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            792..898
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            899..935
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1019
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1119
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1195
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..2176
                     /gene="Scly"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ggagtctaaggggtgctcagtcttggggttaccggccagcgatcagggagatggacgtggcgcggaatggcgcgcggggcagtgtggagagcccgcccaacaggaaggtctatatggactacaatgccaccacacctctagagcccgaagtcatccaggctgtgacggaagccatgaaagaagcctggggaaaccccagcagctcatatgtagcaggtaggaaggccaaggacattataaacacagctcgggcaagcctggccaagatgatagggggaaagccccaggacatcatcttcacttctgggggcaccgagtccaataatttagtaatccactctacggtgagatgcttccatgagcagcagaccctgcaggggcgcacggttgaccagatcagccctgaggaggggaccaggccccattttatcacttgcacggtggagcatgactccatccggctgcccctggagcacctggtggaagatcaagtggcagaggtcacttttgtcccagtgtcgaaggtaaatgggcaggtagaagttgaggatatcctggccgctgtccgtcccacaacatgccttgtgaccatcatgttggccaataacgagactggtgtcatcatgcccatctctgagatcagtcgtcgcattaaagccctgaaccagatccgggctgcctcggggctaccccgagtcctggtgcacacagacgctgctcaggcactggggaaaaggcgagtggatgtcgaggacctgggcgtggacttcctgaccatcgtggggcataagttctacggccccaggatcggtgctctgtatgtgcgaggggtcggtaaacttacccccctgtaccccatgctgtttggaggtggacaagagcggaatttcaggccagggaccgagaacacgcccatgattgctggccttgggaaggctgcggatctggtgagtgagaactgcgagacttatgaggcccacatgagagacatccgggattacctggaggagaggctggaggctgaatttggtaagagaatccatttgaacagccgctttccaggagtggagaggcttccgaacacctgcaacttttccatccaggggtcccagcttcgagggtacatggtccttgcacagtgccagacactgctggccagcgtgggagcttcatgccactcggatcatgaggaccggccatccccagtgctgctgagctgtggtattcctgtggatgtggcccggaacgcagtccggctcagtgtgggccgcagtaccactagggctgaagtggacctcatcgtgcaggacctgaagcaggccgtgaaccagctggaaggcccggtctagagctagcagccactctctccctcagcggccagctggactttggccactcaatccataggaaaccgcttgtgaaagttcctggcccctaagctgtcccttctcaagagctgcactgagatgactgtcctactatggggtgcaaagtgacagggggctgcccactgcacagtatcccactctagtgcccatgcttataagctcaggacagccgtgtcacataggacgtcccctgtcacctccgacaagccacaggggaataggaatatcccagttatcaggcagcctgacaggaccacccgaccccagaagacactttgatcagaagacaaaacctacgtcttggtctactgtcacttagctgctcatgtgagaataggaagttgatttcgtctccacccatggcctcccgtgtgcacctacagacaacacccctcccccatgctgccctgcccttctcctctgccctcagcctgccactgcccacctgcctccctggcctgatggtccccagacgaggtctcatgccttgttcccagaggtcacagctcaccctgtcttctacacagggatggctgccgggaggctgctgcctgatctttgccaaagtgggaaatccggtcatttctcactcagattcaattttaattttatctgcaaagggttctgtcatcttgaggatgtgtggcacacatcacacccatgtttatataggaaatgtcttcttggaggacacttttagcacgtgggtctgtgggccaaaggatgacgcagcatgtggaaacagttgtcagaagttaaatgaactaatgaaagatgcctgcacttgc

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