LOCUS       NM_178706               2964 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus sialic acid binding Ig-like lectin H (Siglech),
            transcript variant 1, mRNA.
ACCESSION   NM_178706 XM_133500
VERSION     NM_178706.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Minutti,C.M., Piot,C., Pereira da Costa,M., Chakravarty,P.,
            Rogers,N., Huerga Encabo,H., Cardoso,A., Loong,J., Bessou,G.,
            Mionnet,C., Langhorne,J., Bonnet,D., Dalod,M., Tomasello,E. and
            Reis E Sousa,C.
  TITLE     Distinct ontogenetic lineages dictate cDC2 heterogeneity
  JOURNAL   Nat Immunol 25 (3), 448-461 (2024)
   PUBMED   38351322
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Ahodantin,J., Wu,J., Funaki,M., Flores,J., Wang,X., Zheng,P.,
            Liu,Y. and Su,L.
  TITLE     Siglec-H-/- Plasmacytoid Dendritic Cells Protect Against Acute
            Liver Injury by Suppressing IFN-gamma/Th1 Response and Promoting
            IL-21+ CD4 T Cells
  JOURNAL   Cell Mol Gastroenterol Hepatol 18 (3), 101367 (2024)
   PUBMED   38849082
  REMARK    GeneRIF: Siglec-H[-/-] Plasmacytoid Dendritic Cells Protect Against
            Acute Liver Injury by Suppressing IFN-gamma/Th1 Response and
            Promoting IL-21[+] CD4 T Cells.
REFERENCE   3  (bases 1 to 2964)
  AUTHORS   Valente,M., Collinet,N., Vu Manh,T.P., Popoff,D., Rahmani,K.,
            Naciri,K., Bessou,G., Rua,R., Gil,L., Mionnet,C., Milpied,P.,
            Tomasello,E. and Dalod,M.
  TITLE     Novel mouse models based on intersectional genetics to identify and
            characterize plasmacytoid dendritic cells
  JOURNAL   Nat Immunol 24 (4), 714-728 (2023)
   PUBMED   36928414
REFERENCE   4  (bases 1 to 2964)
  AUTHORS   Hattori,Y., Kato,D., Murayama,F., Koike,S., Asai,H., Yamasaki,A.,
            Naito,Y., Kawaguchi,A., Konishi,H., Prinz,M., Masuda,T., Wake,H.
            and Miyata,T.
  TITLE     CD206+ macrophages transventricularly infiltrate the early
            embryonic cerebral wall to differentiate into microglia
  JOURNAL   Cell Rep 42 (2), 112092 (2023)
   PUBMED   36753421
REFERENCE   5  (bases 1 to 2964)
  AUTHORS   Wang,X., Liu,M., Zhang,J., Brown,N.K., Zhang,P., Zhang,Y., Liu,H.,
            Du,X., Wu,W., Devenport,M., Tao,W., Mao-Draayer,Y., Chen,G.Y.,
            Chen,Y.E., Zheng,P. and Liu,Y.
  TITLE     CD24-Siglec axis is an innate immune checkpoint against
            metaflammation and metabolic disorder
  JOURNAL   Cell Metab 34 (8), 1088-1103 (2022)
   PUBMED   35921817
REFERENCE   6  (bases 1 to 2964)
  AUTHORS   Angata,T.
  TITLE     [Functions and molecular evolution of Siglecs, a family of sialic
            acid receptor proteins]
  JOURNAL   Tanpakushitsu Kakusan Koso 53 (6), 733-738 (2008)
   PUBMED   18453149
  REMARK    GeneRIF: are a family of sialic acid receptor proteins expressed in
            immune cells, recognize their ligands and play a role in the signal
            transduction.
            Review article
REFERENCE   7  (bases 1 to 2964)
  AUTHORS   Blasius,A.L. and Colonna,M.
  TITLE     Sampling and signaling in plasmacytoid dendritic cells: the
            potential roles of Siglec-H
  JOURNAL   Trends Immunol 27 (6), 255-260 (2006)
   PUBMED   16679063
  REMARK    GeneRIF: A member of the sialic acid binding Ig-like lectin
            (Siglec) family, Siglec-H, has recently been identified as a
            specific surface marker for pDCs in mice.
            Review article
REFERENCE   8  (bases 1 to 2964)
  AUTHORS   Zhang,J., Raper,A., Sugita,N., Hingorani,R., Salio,M.,
            Palmowski,M.J., Cerundolo,V. and Crocker,P.R.
  TITLE     Characterization of Siglec-H as a novel endocytic receptor
            expressed on murine plasmacytoid dendritic cell precursors
  JOURNAL   Blood 107 (9), 3600-3608 (2006)
   PUBMED   16397130
  REMARK    GeneRIF: Siglec-H was expressed specifically on plasmacytoid
            dendritic cell precursors in bone marrow, spleen, blood, and lymph
            nodes.
REFERENCE   9  (bases 1 to 2964)
  AUTHORS   Blasius,A.L., Cella,M., Maldonado,J., Takai,T. and Colonna,M.
  TITLE     Siglec-H is an IPC-specific receptor that modulates type I IFN
            secretion through DAP12
  JOURNAL   Blood 107 (6), 2474-2476 (2006)
   PUBMED   16293595
REFERENCE   10 (bases 1 to 2964)
  AUTHORS   Stefan,M., Claiborn,K.C., Stasiek,E., Chai,J.H., Ohta,T.,
            Longnecker,R., Greally,J.M. and Nicholls,R.D.
  TITLE     Genetic mapping of putative Chrna7 and Luzp2 neuronal
            transcriptional enhancers due to impact of a transgene-insertion
            and 6.8 Mb deletion in a mouse model of Prader-Willi and Angelman
            syndromes
  JOURNAL   BMC Genomics 6, 157 (2005)
   PUBMED   16280085
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160534.5.
            
            On Feb 25, 2022 this sequence version replaced NM_178706.5.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            CCDS Note: The coding region has been updated to represent a
            protein that contains an additional segment in the coding region
            and encodes a longer protein which maintains the original reading
            frame and is more supported by the available transcript data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK047813.1, DQ208935.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-496               AC160534.5         56920-57415         c
            497-775             AC160534.5         56476-56754         c
            776-823             AC160534.5         56262-56309         c
            824-917             AC160534.5         53945-54038         c
            918-998             AC160534.5         53059-53139         c
            999-2964            AC160534.5         46193-48158         c
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 33.42 cM"
     gene            1..2964
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="sialic acid binding Ig-like lectin H"
                     /db_xref="GeneID:233274"
                     /db_xref="MGI:MGI:2443256"
     exon            1..496
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    13..15
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="upstream in-frame stop codon"
     CDS             61..990
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; SIGLEC-like protein"
                     /codon_start=1
                     /product="sialic acid binding Ig-like lectin H isoform 1
                     precursor"
                     /protein_id="NP_848821.2"
                     /db_xref="CCDS:CCDS21313.2"
                     /db_xref="GeneID:233274"
                     /db_xref="MGI:MGI:2443256"
                     /translation="

MLLSRLLPLLWVLKWASGNCFILPREVIGTYPSLTLELQSVTVQEGLCVLVPCHLIEYPMNRWSDAAFGYWFHEGENTHPDSLVATNKPYQLVQKEMQSRFHFSGDCSLDIRDAQKGDNGSYFFRLEKGGKTWNFCEERISVHVTDLTHSPNISIPQTLELGRPTNVMCSVPWACERGTPPIFSWMSAALISLGPTTTLSSVLTLTPRLQDHGTNLTCQVTFPGAGVTVQKTIQLNVTYNSQNSTAHVSGRVGPGKPRPLADVVQVAMGEAAIKFLLLGICFFFLSKRSHTKRVERPATHMDQAHAVMD"

     sig_peptide     61..114
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
     misc_feature    178..495
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Immunoglobulin domain; Region: Ig; cl11960"
                     /db_xref="CDD:472250"
     misc_feature    205..219
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand B [structural motif]; Region: Ig strand
                     B"
                     /db_xref="CDD:409377"
     misc_feature    262..276
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand C [structural motif]; Region: Ig strand
                     C"
                     /db_xref="CDD:409377"
     misc_feature    379..393
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand E [structural motif]; Region: Ig strand
                     E"
                     /db_xref="CDD:409377"
     misc_feature    421..438
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand F [structural motif]; Region: Ig strand
                     F"
                     /db_xref="CDD:409377"
     misc_feature    460..480
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand G [structural motif]; Region: Ig strand
                     G"
                     /db_xref="CDD:409377"
     misc_feature    511..768
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Immunoglobulin domain; Region: Ig; cl11960"
                     /db_xref="CDD:472250"
     misc_feature    553..567
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand B [structural motif]; Region: Ig strand
                     B"
                     /db_xref="CDD:409579"
     misc_feature    604..618
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand C [structural motif]; Region: Ig strand
                     C"
                     /db_xref="CDD:409579"
     misc_feature    661..675
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand E [structural motif]; Region: Ig strand
                     E"
                     /db_xref="CDD:409579"
     misc_feature    703..720
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand F [structural motif]; Region: Ig strand
                     F"
                     /db_xref="CDD:409579"
     misc_feature    748..759
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="Ig strand G [structural motif]; Region: Ig strand
                     G"
                     /db_xref="CDD:409579"
     exon            497..775
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     exon            776..823
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     exon            824..917
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     exon            918..998
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     exon            999..2964
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2942..2947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="hexamer: AATAAA"
     polyA_site      2964
                     /gene="Siglech"
                     /gene_synonym="6430529G09Rik; Siglec-H"
                     /note="major polyA site"
ORIGIN      

agttcctatctgtgagccctccagtttgcttactcacaggagcccaggccatctgctgggatgctgctgtcccgactgctgcccttgctctgggtgcttaagtgggcatctggaaactgctttattctccctagagaggtcataggtacgtatccgtcactgacactggagttacagtctgtgacggtgcaggagggtctgtgtgtccttgtgccctgccacctcattgagtaccccatgaacaggtggtcagatgctgcctttgggtactggttccatgaaggggaaaatacacaccctgactctctggtggctacaaacaagccatatcaactagtgcagaaggagatgcagagccgattccacttctctggggactgctccttggacatcagagatgcacaaaagggtgacaacggttcttacttcttcagactggaaaaaggaggcaaaacatggaatttctgtgaggaaaggatctctgtgcatgtgacagacctcactcacagccccaacatcagcatcccacagacactggagcttggtcgtcctaccaatgtgatgtgctctgtgccctgggcttgtgaacggggaacaccccccatcttttcctggatgtcagctgccctcatatccctgggccccacgaccacactctcctcagtgctgaccctcactcccaggctccaagaccatggaaccaacctcacctgtcaggtgaccttccctggagctggtgtgactgttcaaaagactatccagctcaatgtcacctataattcacagaactccacagcccatgtctctggaagagttggtccagggaaaccaagacccctggcagatgtggtacaggtagccatgggagaagcggctatcaaattcctgcttcttgggatctgcttcttcttcctcagcaagagatcccatacgaagagagtggagagaccagcaacacacatggatcaggcacacgctgtcatggattagtctctcaggctgtgaagatggctggcattacaccagggatccaccactctcaagtcaagaaagtcatggatgatggtgccaactggactgtctccagacctattaaaatgaaattttagtcttgctcttctactctctgctcctctacatcctcttaagtcttgtgttttattctatgggtatgaatatagggttgtggcccctcccccacagccctcaggcctggaactagttagtaactgagaaagatggatacagggacggtgttaaccagtgtgactataaaccactaacccataatcatcttgatctctgctcactgatgcccactttaagtgtttggttgcatttaaaaagcaaagatatgggagtttatcttttgagatagaaatgcagtgtttatcattctattcctgcactgaagctgggccccttggttaccagaaacaagagagtttccactgcaaaaattggtgttatgagactaaggcttcctgaaatgtccttttaaaaggttctggcctttggaaagcctgggaaaatcagactctatgctacaggaaggaacagaatctatcaggggatagagttaattattcccaggcaaaaatcagtcaggattgctggttatccttaggcacccagcactcaaactacataggataagagcgaaccacactgtcctcccatagactgacaaaactcactgtgactggggcagcccaaattaacattagagaatttacaagagactaaaatgtgtctaatatccaaaaccttcaagctacacacttccttattctgatgcctgtccttcagccttgtgggctaactcatctggaaatcagcaatactactgagactacttgatgaacgtgtactaatggtttgactaaaagtgcctcttctgatgctttccaaactaagtagacagattgatcaggccttgtaggtattaaaagcttctgcttctgaattaaaacaacagttaaattcccttgcagagacatcctccaaagttgaagaggctttgacttagttctcctgagataaaggaaactatgtatggctttgaaagaaacctgttatctctatgctaattattcaagagtatttagaaagaaaaaaaaacctaactcaagtttgggaaaacaaaaaaacaaaaacaaaaacctcagtactctggaggcaaagacaggaaggtctctgtgagtttgaagccagcctaatctacagagtgaggtctaggacagccagagctacacagagaaactctgtctcaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagaaagagaaaagaacaaagagaagaaaaaggaagaaagatagaaagaatgagtaaaaacaaaggaaggaatgaaagaaggaaggaacacaaaaagagacatgacaaagaccaaaaaaaaaaaaatcacacaatatcagtatttgttcttttggtcaccctggctagctactctgctatctacactggctgagcctttaagtcacattctgattaggttaacattgggatcttgtatcttaaactaaaacaggataagtccacaacttgactcattcacaaagaccagtggggaaagcaacagggtcccagaccttagtaggccccaagaccttagcactcctgactccatgacagaagtaccactcaggctatagaactcagcatttaaacagtcacaccagccaaaatgaaaacaaagacctcattcatcaaagtccatagctctgggaaaggctctaaaggtactaacctagctttttggcttctgtagttctgcttctggctaactgttcttgttaactgaagcatgttaacagctcaccctgagaaaggctcagtggtacacgagcatcccaaacatccagtgtcccactgtagtcacctgccgactaataaagatcttctgttggctta

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