LOCUS       NM_153178              13793 bp    mRNA    linear   ROD 08-NOV-2023
DEFINITION  Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.
ACCESSION   NM_153178
VERSION     NM_153178.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Feng Z and Wu J.
  TITLE     hsa_circ_0129047 Upregulates LYVE1 to Inhibit Hepatocellular
            Carcinoma Progression by Sponging miR-492
  JOURNAL   Dis Markers 2023, 6978234 (2023)
   PUBMED   37810197
  REMARK    GeneRIF: hsa_circ_0129047 Upregulates LYVE1 to Inhibit
            Hepatocellular Carcinoma Progression by Sponging miR-492.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Li Z, Jin T, Yang R, Guo J, Niu Z, Gao H, Song X, Zhang Q, Ning Z,
            Ren L, Wang Y, Fan X, Liang H, Li T and He W.
  TITLE     Long non-coding RNA PFI inhibits apoptosis of alveolar epithelial
            cells to alleviate lung injury via miR-328-3p/Creb1 axis
  JOURNAL   Exp Cell Res 430 (1), 113685 (2023)
   PUBMED   37330182
  REMARK    GeneRIF: Long non-coding RNA PFI inhibits apoptosis of alveolar
            epithelial cells to alleviate lung injury via miR-328-3p/Creb1
            axis.
REFERENCE   3  (bases 1 to 13793)
  AUTHORS   Fan B, Chopp M, Zhang Y, Wang X, Kemper A, Zhang ZG and Liu XS.
  TITLE     Ablation of Argonaute 2 in Schwann cells accelerates the
            progression of diabetic peripheral neuropathy
  JOURNAL   Glia 71 (9), 2196-2209 (2023)
   PUBMED   37178056
REFERENCE   4  (bases 1 to 13793)
  AUTHORS   Shui B, Beyett TS, Chen Z, Li X, La Rocca G, Gazlay WM, Eck MJ, Lau
            KS, Ventura A and Haigis KM.
  TITLE     Oncogenic K-Ras suppresses global miRNA function
  JOURNAL   Mol Cell 83 (14), 2509-2523 (2023)
   PUBMED   37402366
REFERENCE   5  (bases 1 to 13793)
  AUTHORS   Kshirsagar A, Doroshev SM, Gorelik A, Olender T, Sapir T, Tsuboi D,
            Rosenhek-Goldian I, Malitsky S, Itkin M, Argoetti A,
            Mandel-Gutfreund Y, Cohen SR, Hanna JH, Ulitsky I, Kaibuchi K and
            Reiner O.
  TITLE     LIS1 RNA-binding orchestrates the mechanosensitive properties of
            embryonic stem cells in AGO2-dependent and independent ways
  JOURNAL   Nat Commun 14 (1), 3293 (2023)
   PUBMED   37280197
  REMARK    GeneRIF: LIS1 RNA-binding orchestrates the mechanosensitive
            properties of embryonic stem cells in AGO2-dependent and
            independent ways.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13793)
  AUTHORS   Lu J, Qian J, Chen F, Tang X, Li C and Cardoso WV.
  TITLE     Differential expression of components of the microRNA machinery
            during mouse organogenesis
  JOURNAL   Biochem Biophys Res Commun 334 (2), 319-323 (2005)
   PUBMED   16036130
REFERENCE   7  (bases 1 to 13793)
  AUTHORS   McKee AE, Minet E, Stern C, Riahi S, Stiles CD and Silver PA.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 13793)
  AUTHORS   Liu J, Carmell MA, Rivas FV, Marsden CG, Thomson JM, Song JJ,
            Hammond SM, Joshua-Tor L and Hannon GJ.
  TITLE     Argonaute2 is the catalytic engine of mammalian RNAi
  JOURNAL   Science 305 (5689), 1437-1441 (2004)
   PUBMED   15284456
  REMARK    GeneRIF: essential for mouse development and cells lacking
            Argonaute2 are unable to mount an experimental response to siRNAs
REFERENCE   9  (bases 1 to 13793)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K and Saigo K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   10 (bases 1 to 13793)
  AUTHORS   Carmell MA, Xuan Z, Zhang MQ and Hannon GJ.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161581.3 and AC118640.8.
            
            On Jul 1, 2022 this sequence version replaced NM_153178.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096465.1, SRR13948564.4563749.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AC161581.3         105098-105265       c
            169-364             AC118640.8         34597-34792
            365-485             AC118640.8         43049-43169
            486-667             AC118640.8         50120-50301
            668-804             AC118640.8         52702-52838
            805-939             AC118640.8         54074-54208
            940-1027            AC118640.8         54628-54715
            1028-1175           AC118640.8         55851-55998
            1176-1295           AC118640.8         56913-57032
            1296-1418           AC118640.8         57163-57285
            1419-1552           AC118640.8         57706-57839
            1553-1737           AC118640.8         59914-60098
            1738-1897           AC118640.8         61666-61825
            1898-1988           AC118640.8         64769-64859
            1989-2183           AC118640.8         67260-67454
            2184-2318           AC118640.8         69341-69475
            2319-2420           AC118640.8         72689-72790
            2421-2620           AC118640.8         73798-73997
            2621-13793          AC118640.8         74146-85318
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 33.92 cM"
     gene            1..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="argonaute RISC catalytic subunit 2"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
     exon            1..168
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    21..23
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="upstream in-frame stop codon"
     CDS             147..2729
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /EC_number="3.1.26.n2"
                     /note="argonaute 2; Piwi/Argonaute family protein meIF2C2;
                     eukaryotic translation initiation factor 2C, 2; argonaute
                     RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_694818.3"
                     /db_xref="CCDS:CCDS37098.1"
                     /db_xref="GeneID:239528"
                     /db_xref="MGI:MGI:2446632"
                     /translation="

MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    165..2723
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1080..1097
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1308..1310
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    1323..2600
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1719..1847
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1734..1736,1746..1748,1782..1793,1800..1802,
                     1824..1826,1833..1835,1845..1847,1857..1859)
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    1908..1919
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    order(1938..1940,1944..1946,2154..2156,2568..2570)
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2097..2129
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    2247..2249
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000255|HAMAP-Rule:MF_03031; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); hydroxylation site"
     misc_feature    2274..2279
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2406..2432
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2517..2585
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG0.3);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2619..2621
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2631..2633
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2640..2642
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     misc_feature    2649..2651
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9UKV8; propagated from
                     UniProtKB/Swiss-Prot (Q8CJG0.3); phosphorylation site"
     exon            169..364
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            365..485
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            486..667
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            668..804
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            805..939
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1027
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1175
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1295
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1418
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1552
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1737
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1897
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1988
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2183
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2318
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2420
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2620
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..13793
                     /gene="Ago2"
                     /gene_synonym="1110029L17Rik; 2310051F07Rik; Eif2c2;
                     Gerp95; Gm10365; mKIAA4215"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gccgccccggccgcgtcgggtaaacctgttctgcgaggcggccgcgccggggcggatcgagcggccggcggctcctcgcggctcgcggcgtcggggcccgtggagcgcgcggccggccctcggccgcggccctcggcaacgccaccatgtactcgggagccggccccgttcttgcttctcctgctccgacaacatcacccatcccaggatatgccttcaaacctccacctcggccggacttcggcaccaccgggagaacaatcaaactacaggccaatttctttgaaatggacatccccaaaattgacatctatcactatgaattggacatcaaacctgagaaatgccctcggagagtgaacagggaaattgtggagcacatggtccagcactttaaaactcagatcttcggggaccggaagccagtgtttgatggaaggaagaatctgtacacagcaatgccccttccgatcggcagggacaaggtggagctggaggtcacgctcccgggagaaggcaaagatcgcatctttaaggtatccatcaagtgggtgtcgtgcgtgagcctgcaggcgttacacgatgcactttcggggcggctgcccagcgtccccttcgagacgatccaggccctggacgttgtcatgaggcacttaccatccatgaggtacacccctgttggccgttccttcttcactgcatctgaaggctgttccaaccctctgggtgggggcagagaagtgtggtttggcttccatcagtccgtccgaccttctctttggaaaatgatgctgaatattgatgtatcggcaacagcgttttacaaggcacagccagtgatcgagtttgtttgtgaagttttggattttaaaagtattgaagaacaacaaaaacctctgacagattcccaaagggtaaagtttaccaaagaaatcaaaggtctaaaggtggagataacgcactgtggtcagatgaagaggaagtaccgtgtctgcaatgtgacccggcggcctgccagtcaccaaacgttcccactgcagcaggagagtgggcagacagtggagtgtacagtggcccagtacttcaaggacaggcacaagctggttctgcgctacccccacctcccgtgtttacaagtcggacaggagcagaaacacacctaccttcctttggaggtctgtaacatagttgctggacagagatgtataaaaaaattaacagacaatcagacctcaaccatgatcagagcaactgccaggtcagcacctgatcgccaagaggagatcagcaaactgatgcgaagtgcaagtttcaatacagacccatatgttcgtgaatttggaatcatggtgaaagatgagatgacagatgtgactgggcgggttctgcagccgccctccatcctctacgggggcaggaacaaagcaattgccacccctgtccagggtgtgtgggacatgcgaaacaagcagttccacacgggcatcgagatcaaggtgtgggccatcgcctgcttcgctccccagcgccagtgtacagaagtccatctcaagtccttcacagagcaactgaggaagatctcgagagatgctgggatgccgatccagggtcagccctgcttctgtaaatacgcacagggtgcagacagtgtggagcccatgttccgacacctgaagaacacatacgctggcctccagctggtggtggtcatcctgcctggcaaaactcctgtgtatgcggaagtcaagcgtgtgggagacacagtgctggggatggccacacagtgcgtccagatgaagaacgtgcagaggaccacgccacagaccctatccaatctctgcttaaagatcaatgtcaaactgggaggcgtcaacaacatcctgctgccacagggcaggcctccagtgttccagcaacctgtcatcttcctgggagccgatgtcacccacccaccagctggggatgggaagaaaccttctattgctgccgtcgtgggcagcatggacgcacaccccaaccgctactgtgccaccgtgcgcgtgcagcagcaccgacaggagatcatccaggacctggctgccatggtacgagagttgctcattcagttctacaagtccacccgcttcaagcccacccgcatcatcttctaccgcgatggcgtctccgagggccagttccagcaggttctccaccatgagctcctggccatcagagaggcctgcatcaagctggagaaggactatcagccaggaatcacgttcatcgtggtgcagaagcggcaccacacacgcctcttctgcacagacaaaaatgagcgggttgggaagagtgggaacattcccgcaggcacaaccgtggacacgaagatcacccaccccactgagtttgacttctacctgtgcagtcacgccggcatccaggggacaagccgtccttcccactaccacgtgctttgggatgacaatcgtttttcttctgatgagctgcagattctgacctaccagctgtgtcacacctacgtgcgctgcacacgctctgtgtcaatacccgcgccagcttactatgctcacctggtggccttccgagccaggtaccacctggtggataaagaacatgacagcgctgaaggaagccatacctctgggcagagcaacgggcgagatcaccaagcgctggccaaggcggtccaggtccaccaggacacactgcgcaccatgtactttgcttgacctgttgtattgtttagcgattgtgtaccaagttggattcacacgagaccagctacactcagaccaacagatgcccagcccttctgtgacagccagcatcgaacatgagacgtctttggttttattagatcctccgttttccagaatgccttccgtcccagatgtcagacttggattttgagctgcaggcctgtatgagaccccagtgtaggaaacatggtttgccaaacctataagctgctcactgagagtcccgtttcctaaaaacaaaacaacaacaaaatgtcctaaaaccagtctatgaactctcagggctttaaaacactttaatttatttggtcatccaatttacttgtttttaacacctgattctcactgaaaccgatgggctccatctagctgtgaaggctctgagcatgaaagccacaccatttccattggggttttaaagcctggccctaagaagttgtcactaatgttgagttctaagggatgccatgcgccctgcagccccagggcgtatggctgctaggtctccacagtgtctctcgaggtcactgcctgccctcgaggcctgaacaaaggcacagtggccaaggagatggtcacgtgtgtttacggcagtgacaagtccgagagattggcagacaagtgccattttgataaaaatttatttaaaaagagaaaaaaagacaatatactgacagtctaatcataagatttgtcctgtaggactgtgacatttattttccaaagtttctaaagaatacgggtctgtggtgataaatacagtacaatcctttttcactgttaatgtctttaatgtaagagaagaatatatgtatctggtttgcagtattaatgtggtaggtagatgctgtttccctttcgttgacgaggggcgcttcttgtgacctgcttcacatatcaggagctgtgtggagcctggagacagtccacctcttgtggcttttagtgcttaagtagaggaatatgcaactggcttcatgtaccagcgatgccccctcctccctccccacccccagtgtggactgtgtagcctgtacctctggaaggacagtgcgcttgccagtaacctgccgtcctccctgcctcgtccatgtgtgtcgctcagccgatgaagcacaaatggaatttatttattcttccattgctgggaaacaagttcacgtaagagtgcacttctctcagtgtgcagttatggaaggtgggtttcataggatctatgcactgaagtggagagagagctgcccatagcggctctgccagcatgggactggcaggtaggaatggactgtgcccgaggacctgaccgtgggcaaggccagcagggaaggcacagactttctctgggtgttgtttgcttctgggccccagtgtgggttttccagcccagagaccattgaactgagaaggggccactacgagtggctctgcggatggcagcagccacgtcagtgtgtgtgtcctgtggggctttctgtgcttgtcccgaagctgagcaagcatcgggctcgctccgttccacatacgcgtgcgttcccagaaggagacgagccttctcttgtgtgctgctctgtgctctgtgggttttttctacccctgctgatgggcctcatcccccagctctgcgtcatttacctctgaccctgtggaggagggagggtgcccaccctctcccctgccatggcacctcatgctagaggacacagtaccacctttgccccgttccccatctgctctggagctggtgggaagggcagacgcagttacctgggaagtcacaccctcagcaggtgtgtgtatccgtgtatgcatgtgggtgtcttactaatgacaatagtctcttcttgctgtgtctttagaaatcttttaaagtaacaggaccccccctcccccccagtctaagaagcactgggactgcctgctatcccattggcaagtggcaccttctcaaactgtttccaaaacgatagaaaaacactcagttggcctaacatggtcaggctagggaactggttaaactagtgaacttttagaatctgaaattggattgttttcaaccattcttagaaccgcgtgtttctaaggtgtgtcttcagccccttgccatgggatcttagtcatgcaagtgagttttttagaatgcagattctcacctgctgccagcttcttggatctggcactgtgttcgaggccacccctcaacccccaccgtggctctgtaggcgctttctgaggccgtccccccttatgatgccagggggacaggaaggaacccctatcagagctcacacctaggaacacttgtgggctggggcaaaaaatcttgagctgggagttttgttttgatcaccttgaaggataggttttctttctgatgggccaaagtactggaagcaaagtactttctgtagtagcccatgcttacttgtgacacttttaatatgaaatgtatccccagaccaaatgtccctaattgagttagatacacaccatttacatgtctgatggtgcagagctcataaatggctatagttccaatttctcaggtaaataattttccccaattcccgccctccccttattattaagattagtttcgagacagtgtgagacctcagagtgcggtagcagctccggccatcttctgcaggagccagctttttgtgtgtatctgtacccaagactggtgacaggtgccgttttctcctgaggtgatacctggccaagtttgacgactgcccacttgctagctgtgttcccaaccccactgttaagtaaggtgtgccttgctttaagcaaactgaatgtgattgtgttgtgctctgtatccatttggaaggaaaactaggatgtgcctgtctctttaagagaggctacccaattagcctttcatggtgggactctcctagtagtacactgtaagatactcactcttaaaaacccatcatgacatccatccctttaccactgtgtgcaccatgcagcaggtccctccctccccaagctgatgaagcaatgcaaaactctagagagacatcgaggccccattccatagatttagcccttagtgatactagcaaaactcccaccctgtcactgcgtgttttaagcccagattctcctagtccccatcaagtctgaaaccgagcacgcctgtacttctagtcaggggctgttgggttttggcttccttctcccttttggggaaatcctcacatgcacacttgataccactttgggtctgctctgccttctccagatttggcctcttggccactcctcacacccacccgcttgctggcctccaggccacagggcactgccagtgaggagactgctagctccgtgctgtctgcagctggcccaacatctgtatcctaatgtttacctgtcaccgagcggcgcttgatagaaagatggcccctgcagcgggcttgggcaggtttggtttttttggttttggttttgtttttccattgtcttgttcttgtgttattttattggctttaaatgcaaatgttgctatttattttttcctctctctcctgctctggtttgactaagctgatctggctatcccaatgcatgtggtacctgatagatggatatggtagagattatttcccctgccttttataactgtcccagtgtttaccttcaggcttgctaatgctttatttttggaagccacagatgtagtttttctggatgaattgcttttcctatagatgcagcggtatgcttttgcattgatctgcagggtgatttgaattttatttttacaagcagtttttcaatcaaattctatatttgatacaatgtgttaaggttagaccagcccgaagtcatgcagctggggacccctgagtgtccaggaggccttagggctgggtggcctctcttggtagtccctgtggtggtagcactggtcacaggaacctgcagggtccttcagcatggccctgaactcttggattgttacaaggattttttttttttcatgggcattttgctacacagacagtggacaatcccggtggccccagacaagccataagcaacctttctgtgacctccccctgactcatctctcacacccatgagaaaaggaatgtgccctgagtagaagctacacttccttggtgttcatctgcctcacttgtgaatgcagatattttgtttgttttaagcccttccctgcagtaaccatgctggctggctctcacataggcagagcacacgtgactcttgtcacaagcgccccgttgcaggcatgccgactcctgtaagttgcactgatctctcaacagtttcctttattgcgtccaactccccatctgctgtactcatttgaagtgattgtaaacagattcacagtggcaagagtgggacattgcctcacgtctgccatcccaacacacatttcagtattacatgctcgagtgtgcgggctgggctcttactctctcgagcattgtgggctagccttgtctcaacactctgacaagaagttctccctctctatacaggacagcatgctctttgagacctgaaggcttgaatgtaacagattagttaggagtctttgtggcgctttggtctgtttagttttcttgtgatctgcaaggccccccaaaagtatgtccttctagttccgtgtggcctttgtcagcatggacagcatatgaatagcccagtcagtgttaacaggccattgctacattctttggtcagcccctcaacttaaagttgtgaggtcactgctgtgtcagactgtcatttgtggtccatccatgggagacctaaagcctggtgcagccctttatgccatgtgaccttgctgggacctaagtacatggtctgtttagccccacgtggactgtctgtagtcagggtgcccagagtctgaaacaaaaggaattattacactcaaaccgcgggggaggggggtactggggacggacggacagacagacagacagacagacagacagacagacagtgccattgcagacattgcagagccctccctcaccttgtgtctttggatttgttcagccattttatttgtctcaagttgaatgtaccggcttagccaaatcactacagaaggtggtttctttaaaccttttagacagatgcataaaagctacctctgacttctcatagatttgtgtttgctctgaagcatcgctatccacactgaatatagccaaaatatcgcctgaataaaccgagttacgaactcctctgttcagttctggcttgaaaatgtgtgagtgccatactgtgatctggggccgcagctcctctgctgccactgacccagggactcctcagcaccacccagctgcaagatcctagcgtccgagaggctggtagactggcttctctgcatggggaacgtgcaccctccaggctggcgggcgtgactgcgcagggccgcgtgcggctgagcctggagatggtctgttcctgcctggctttgctttcctgttgaatcgtgtaagaaggcatctttacccctgcttctgaatggattgaaagtggctttcctgctctctcctccctttttgtacactataaactcataaaggtgcttcaacaccgactcttgtgcagataatgtagttaagcagttgcatggattcagatagtgcaggcacagctgaccttgggaccctggtgaaagtgaaggactgaaagccctgcgctccatttggaaggagcaatgggctctgcttgactagaaggtccctttagtgttggcagaacagagatttgtcatggggacctgccacctctgaccaagctgtcctgtggctgtctgctttgctgtccaggaggtcatcctcttacatcatggctccacctgcatctccttggcctctccacctaaagtttccttggaaagtcagcataagatcccagatttccctgtttagcttgagggacagatggacagtaccatgaagggacagattgacagtgtcgtgaaggtcggtgcctggggattgggctgggcttaggggagctcttctaaaggttgtctctgcgccctccccaacctcccccacatacacccccattgtattgttttggggggtgtcatcttccattcttttgacttggacagctcagtacccttaagagccctcacatatttctctgtgtagagaacctgaggcgtggagggtggtggggtgtctgcaacacccctgacctgcaccccaggttacttaaatcccctgacttggctggaatttgaaaatccagggaagcacagggtgctagatatgttacacttagggtcacacccaggtgccgtggtgtaagccacagccctcagggcaccagcacagaggcagccagcctccccactcgtgacatttgggaaggatactaaacaagccagagccctggccgctgcagtattcagctaacacttctttttcccggttgaaaggagaacactatttgaaaccttctaatgccctggtggttctagttcctccgttcctgagggctaaaaggttgaatgagctcctgttttgcttagagctgcctttatttgagtccatttaaaggtgaagcacgggactgtctcctctccctgagtcaccagcaagtaggtgcggagacccttctcattcagtcctttctgtatcttcaagcgccccaacatcagtgggatcacgagagcacccacatcagccatcagtggagtcaagtatacatatggggacagaaattggcaaatttacaggttgtaatggccactccagcctgtgacagggcaggccctccatcctgcacagcaggtggcctctgtcctacctccaggcactggctttactatctggacacctagttgagagccttgaaagtctgttcttggtgtccttttagaatcatgctggggctggtcgttagttgaccctgcccagaagctgtcagccctcagctctttcttgcaaactgtgatgccaccttgtagcctacggctgtggagccccgtcctgggttctttgtgccctgaaatgcaggtgcacctctgctgggggttggtggggcactggcaaaggctacttgctggccttgtgttctctgcccctcagcaagactgtctgttgtagtggaagtaggtttagagggatttggaatcatgtctttgtgaatcgagtgtgacgtcatcaggggtacggtgctacttggtcctaatgttttggaatgcttttccgtcgttgttactccattgttactctctgttctatgacactgagggagactgctcacttaggtgtggtgtcacataggactccagctttgatgtgctgtgcgggtggtgtgattatcatctctgaccttctgaagccaggggtggtacccatccctagctcagtctaattaaatctttcctccctgtcaagtgattgctttgggtggttttaacaggagtagtccagtctggatattctattttcttaattacatagaaaaggcccgtgcagcactagctgcagacagataacacatttaacaattttggttcctcttcaaagccaaatagacctatgttgattaaactccaagagggtacaattcagcattaaattaaaatttgagttgtgaatttgagagggaatttcagtatagttgataaagttaagtggcaagtgagtatttataaaagttataagagaataattgtaagtttctgtacaacaatttggctactgcctcataaatacaggaagagaccgtagccctgaatccgactctctcacctcccacataccttggactacctgcctaatatgtatttgagggggtttaacccttggttttatcaaattttctagttttattttatttttataaaataattgaaaaagaaccaaaaaggaatccagaagttccccagtggctcctcccccagtcttaatcccctcccccccacacacacacacacactcaaatgttttagaagacattttgaatcaaaatgtattccacccatttaacgtttgctactttctgagaagtggagtggctacgagggcctgtttcagatggtactctgtatgtcatggacatgtttgtacctgtacagtggatctgtgtgacgacgtcttgtgcttgcggtttagaaaccacgcagtgctgttttgtaaattgacagtttctgtgtaaactattatttatatcccttggcccctctgtataatttttcatttgctacatggattgtataattaattataaaattatagtacctgctaaacccaattctgaggcttcatgtcattgtctttggtttgataccagtctgatcgggaggctgcccaggccttgcgcaataccagtagtgatgttacttaaagttaagccttaatcagacagtgaatttgtgcatttcagagtatgcaaaaaaaaaaaaaaaaaaaaaagcagagtgagtttcaactgcccaggtcaagatgttcaaccctaactaacaggttccttctggagtgaaaaagtctaatgggttccctctggggtgaaaaaagggccgttacccgggttacactcttccctcccacctgcgctctcccatgtggcgtgatgaaactgatcgtgtgcatttctgtggcagtctatctagagacctttgcagggttgtcttcaaatgtctggtttctctcctctggtctgtgacgagcgagcgtctactgccgggagcacagctggggcgtaggtttcaccaacctgctcagcggccaagctctccgctgttactgccacctgctattgcttctgcatagattataacagatttgtgtcgggtaaaagcatcttcaatcttgaggtaaattagaggggacccagctttcttttacaatgtggatctacctcagttaaacagttgggtgctattaactaagtctgtcaaattaaattggaaagtaaccaaacagtgggacataactaactccacgtgaaacttgaagacatcatttccgtttctttaataatactttttatttattttgtgccttttatgttaaccctaatgcgttttttaaaattcagaatagtcatttttctcttgcgtgtggggagcccaactgaccatacaagcagccatctaactgctagagtatactgggatcctaacctaccctcttgggaaacggagggggaaaatgtgtccaagagcaaatttcacacatcacataccccagctcttacatgcttaccttcatagctttcgcttcatttctgtgccatctgaaggggcaacccccaaaactgacactcgagtgctttgtctctgctttttcagctgtccgtgtctctcttctctggcatgttttctataagactttctttcataaacagcaagtggactgttgtcgatcccatctcctggcttgcacagtgaaggtgctaaacgccaggagcagaggcgggggcaagctcacggttgtctggaggccaggccggaggcggggaggatgtgcacgatgcggcagcccgcggtttatgaagtcaggaacactctggtaagaaaataagatcggagatcgataactttccatgcctttatatttggcagtttacaacttttcctactacggcaacctttttgccataattctttggaggtaacacaatattttgaagtcttatgaatttgattttataccagtgagaagtcaaagttttagcggtctttcctgtagtgtatggttttactcttttaaagtcatctctggattttagaagagaacctattgtacttggtggctccaaaaggtgatttggggagccctgtcccttcccttcttgctctgctcccccccccccctttccctttgcagggtctcaaaaaaggaaaacaagaaaaaaaaatgatggttctagttagatttgtgggggttttttattgttggtttttttttttaagcttccatcaggttgatacttaagctatattttatagaataaatccccctttagtatgctgcatatggatatgaagctcagagctgttgcactttctctgtaatcaatgccctccaactggctcgctggaagcattttcttttaacctaatgtgtactttgggcaggtgatgaaatttacacaggtacctcaagtcaagacctgcgcgtgctgcatttggatttttggttttgttttgttttgcttttctatgtatcattatgttttacttaattttgttctggtgttatgctttttggaccccaatagtctgttgaaagaaaaacctaaaaagttagtatccttgttttctcccccctgcagtatgtggttctaaaggtcaccccccccccccattaggaatctcccccaagcacacccgaggaaaagggtacgacgggcgcttctcagcgggccgtgccattgagatcttttatattcatgctgcttctagtcccttctccacatcacctaagggtgggtacctccattgcgatgttgtggcatcttaacttttacacacagacctcatgggtgtccactgaggaagtagtatgagcctaaagacactggcagcgtgcagcctggcctgggagtggcggccttcgcatctctctctctctcagcactcagccacgctgacctggccgggtggtccgcgtggacaggacagctggtgaagcaagccggatgctcagaacactgcgcgtctcctgtcctgttttaagcttagctgggtcctttgttttcacagcttcctaggttaaatagtttgcactatttttgcaataaattcatgggaaacctaatggctacttatgttgttgttcttactgtgtgtataaactaacactaaaggtttgctatagtgttttcttttcacctccttatgtagccccataacatgcacagaatgctgtaaacctgattaagatatgatgtggatattttataaagttattttgtatggtgtcgattttgttttgccgcatagtgtgtcagcaaaaaataaaataaaataaaattcctcgtatttacagctgccta

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