LOCUS       NM_025416               1251 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus thioesterase superfamily member 5 (Them5), mRNA.
ACCESSION   NM_025416
VERSION     NM_025416.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Zhuravleva E, Gut H, Hynx D, Marcellin D, Bleck CK, Genoud C, Cron
            P, Keusch JJ, Dummler B, Esposti MD and Hemmings BA.
  TITLE     Acyl coenzyme A thioesterase Them5/Acot15 is involved in
            cardiolipin remodeling and fatty liver development
  JOURNAL   Mol Cell Biol 32 (14), 2685-2697 (2012)
   PUBMED   22586271
  REMARK    GeneRIF: Loss of Them5 specifically alters the remodeling process
            of the mitochondrial phospholipid cardiolipin. Them5(-/-) mice show
            deregulation of lipid metabolism and the development of fatty
            liver, exacerbated by a high-fat diet.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK004155.1, AK009228.1 and AV021934.1.
            
            On Apr 9, 2011 this sequence version replaced NM_025416.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046437.1, AK004155.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1076              AK004155.1         2-1077
            1077-1224           AK009228.1         1076-1223
            1225-1251           AV021934.1         258-284
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.52 cM"
     gene            1..1251
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /note="thioesterase superfamily member 5"
                     /db_xref="GeneID:66198"
                     /db_xref="MGI:MGI:1913448"
     exon            1..283
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..907
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /EC_number="3.1.2.2"
                     /note="acyl-CoA thioesterase THEM5; acyl-coenzyme A
                     thioesterase 15"
                     /codon_start=1
                     /product="acyl-coenzyme A thioesterase THEM5"
                     /protein_id="NP_079692.1"
                     /db_xref="CCDS:CCDS17590.1"
                     /db_xref="GeneID:66198"
                     /db_xref="MGI:MGI:1913448"
                     /translation="

MLRTSFQGVARLVRHKALYRSPCLLPRVHLASAFGSSTESLVARFCPEKTDLKDYALPNASWCSDMLSLYQEFLEKTKSGGWIKLPSFKSNRDHIQGLKLPFGLETASDKQDWRLFTRSIQLEGQGYEYVIFFHPSEKKSVCLFQPGPYLEGAPGFAHGGSLAALMDETYSKTAYLAGEGLFTLSLNIKFKNLIPVGSLAVLDIQVEKIEDQKLYMSCIAQSRDKQTVYAKSSGVFLQLQLEEQSQEQ"

     misc_feature    554..871
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /note="The hotdog fold was initially identified in the E.
                     coli FabA (beta-hydroxydecanoyl-acyl carrier protein
                     (ACP)-dehydratase) structure and subsequently in 4HBT
                     (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A
                     number of other seemingly unrelated...; Region: hot_dog;
                     cl00509"
                     /db_xref="CDD:444946"
     misc_feature    order(632..637,728..739)
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /note="active site 2 [active]"
                     /db_xref="CDD:239524"
     misc_feature    order(650..652,659..664,671..673,704..712)
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /note="active site 1 [active]"
                     /db_xref="CDD:239524"
     exon            284..485
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            486..624
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            625..735
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            736..860
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1251
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1229..1234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
     polyA_site      1251
                     /gene="Them5"
                     /gene_synonym="1110007B02Rik; 1110038F21Rik"
ORIGIN      

ccagcccagtcttgcacagtttcttcttcagcaggaactctctgctagaagaaagcccgtgttaggtgtgcctggtccaagcaaggctgcctggcactcacttgtcctctggagtcagccactatttatccaagcaaagccgggatcctggcactcagccatgttgaggacaagtttccagggagtggcaagacttgtccgccacaaagctctttacagaagcccctgtctcctgcccagagtccacctggcctcagcatttggttcctccacagagtccctggttgcaagattctgcccagagaagacagatttgaaagattatgcccttcccaatgccagctggtgttcagatatgctgagtctgtatcaagaatttctggaaaaaaccaaatcaggcggctggattaaactaccctccttcaaatccaacagagatcatatccagggccttaagctcccatttgggctggaaactgcctcagacaaacaggactggcgcctctttaccaggtccatacaactggaaggacaaggctatgaatacgtcatctttttccacccatctgagaagaagtctgtctgtcttttccagccaggcccctacctggagggggcaccagggtttgctcacggagggtcactggcagccttgatggatgagacatattcgaaaactgcatatctggctggagaaggactattcacactaagtctaaacatcaagttcaaaaacttgatccccgtgggctctttggctgtcctggacattcaagtagaaaagattgaagaccagaaactctacatgtcctgcatcgcccagagcagagacaagcagaccgtctatgccaagtcctccggtgttttccttcagctgcagttggaagaacagtcccaggagcaatgaccttgaccttgatgactcagtggccacataacagacaagggtccccaggaatcaggtggcagaggagggagaaaaaggcatttttaccaccccacggtcagtccaagaaccttctgtagttggaatcaaaatcgtccgtaggccacagataactcagcagttaagaaccctagctgctgcttgatttccagcaccaacaaggtggttcacaaccatctgtaactcctgtcccagggaatccaatacccttttctggcctccatggagtaccaggtacacgtccagtgccttccgtacaaacccaacacccgtatacctaaaaataaatgaatgttttaaacctt

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