LOCUS       NM_011375               2446 bp    mRNA    linear   ROD 10-SEP-2023
DEFINITION  Mus musculus ST3 beta-galactoside alpha-2,3-sialyltransferase 5
            (St3gal5), transcript variant 2, mRNA.
ACCESSION   NM_011375
VERSION     NM_011375.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Chowdhury S, Kumar R, Zepeda E, DeFrees S and Ledeen R.
  TITLE     Synthetic GM1 improves motor and memory dysfunctions in mice with
            monoallelic or biallelic disruption of GM3 synthase
  JOURNAL   FEBS Open Bio 13 (9), 1651-1657 (2023)
   PUBMED   37401916
  REMARK    GeneRIF: Synthetic GM1 improves motor and memory dysfunctions in
            mice with monoallelic or biallelic disruption of GM3 synthase.
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Bharathi SS, Zhang BB, Paul E, Zhang Y, Schmidt AV, Fowler B, Wu Y,
            Tiemeyer M, Inamori KI, Inokuchi JI and Goetzman ES.
  TITLE     GM3 synthase deficiency increases brain glucose metabolism in mice
  JOURNAL   Mol Genet Metab 137 (4), 342-348 (2022)
   PUBMED   36335793
  REMARK    GeneRIF: GM3 synthase deficiency increases brain glucose metabolism
            in mice.
REFERENCE   3  (bases 1 to 2446)
  AUTHORS   Arends M, Weber M, Papan C, Damm M, Surma MA, Spiegel C, Djannatian
            M, Li S, Connell L, Johannes L, Schifferer M, Klose C and Simons M.
  TITLE     Ganglioside lipidomics of CNS myelination using direct infusion
            shotgun mass spectrometry
  JOURNAL   iScience 25 (11), 105323 (2022)
   PUBMED   36310581
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2446)
  AUTHORS   Strekalova T, Veniaminova E, Svirin E, Kopeikina E, Veremeyko T,
            Yung AWY, Proshin A, Tan SZK, Khairuddin S, Lim LW, Lesch KP,
            Walitza S, Anthony DC and Ponomarev ED.
  TITLE     Sex-Specific ADHD-like Behaviour, Altered Metabolic Functions, and
            Altered EEG Activity in Sialyltransferase ST3GAL5-Deficient Mice
  JOURNAL   Biomolecules 11 (12), 1759 (2021)
   PUBMED   34944404
  REMARK    GeneRIF: Sex-Specific ADHD-like Behaviour, Altered Metabolic
            Functions, and Altered EEG Activity in Sialyltransferase
            ST3GAL5-Deficient Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2446)
  AUTHORS   Suzuki M, Nagane M, Kato K, Yamauchi A, Shimizu T, Yamashita H,
            Aihara N, Kamiie J, Kawashima N, Naito S and Yamashita T.
  TITLE     Endothelial ganglioside GM3 regulates angiogenesis in solid tumors
  JOURNAL   Biochem Biophys Res Commun 569, 10-16 (2021)
   PUBMED   34216992
REFERENCE   6  (bases 1 to 2446)
  AUTHORS   Takashima S and Tsuji S.
  TITLE     Comparison of genomic structures of four members of
            beta-galactoside alpha2,3-sialyltransferase genes in the mouse
  JOURNAL   Cytogenet Cell Genet 89 (1-2), 101-106 (2000)
   PUBMED   10894948
REFERENCE   7  (bases 1 to 2446)
  AUTHORS   Kapitonov D, Bieberich E and Yu RK.
  TITLE     Combinatorial PCR approach to homology-based cloning: cloning and
            expression of mouse and human GM3-synthase
  JOURNAL   Glycoconj J 16 (7), 337-350 (1999)
   PUBMED   10619706
REFERENCE   8  (bases 1 to 2446)
  AUTHORS   Takashima S, Tachida Y, Nakagawa T, Hamamoto T and Tsuji S.
  TITLE     Quantitative analysis of expression of mouse sialyltransferase
            genes by competitive PCR
  JOURNAL   Biochem Biophys Res Commun 260 (1), 23-27 (1999)
   PUBMED   10381338
REFERENCE   9  (bases 1 to 2446)
  AUTHORS   Fukumoto S, Miyazaki H, Goto G, Urano T, Furukawa K and Furukawa K.
  TITLE     Expression cloning of mouse cDNA of CMP-NeuAc:Lactosylceramide
            alpha2,3-sialyltransferase, an enzyme that initiates the synthesis
            of gangliosides
  JOURNAL   J Biol Chem 274 (14), 9271-9276 (1999)
   PUBMED   10092602
REFERENCE   10 (bases 1 to 2446)
  AUTHORS   Kono M, Takashima S, Liu H, Inoue M, Kojima N, Lee YC, Hamamoto T
            and Tsuji S.
  TITLE     Molecular cloning and functional expression of a fifth-type alpha
            2,3-sialyltransferase (mST3Gal V: GM3 synthase)
  JOURNAL   Biochem Biophys Res Commun 253 (1), 170-175 (1998)
   PUBMED   9875239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127244.3.
            
            On Aug 4, 2022 this sequence version replaced NM_011375.3.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' terminal exon and initiates translation at a downstream
            start codon, compared to variant 1. It encodes isoform b, which has
            a shorter N-terminus, compared to isoform a. Variants 2, 3, and 4
            all encode the same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158594.1, Y15003.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-387               AC127244.3         89481-89867
            388-511             AC127244.3         120137-120260
            512-623             AC127244.3         123925-124036
            624-967             AC127244.3         134013-134356
            968-1154            AC127244.3         138942-139128
            1155-1313           AC127244.3         140892-141050
            1314-2446           AC127244.3         145304-146436
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.24 cM"
     gene            1..2446
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /note="ST3 beta-galactoside alpha-2,3-sialyltransferase 5"
                     /db_xref="GeneID:20454"
                     /db_xref="MGI:MGI:1339963"
     exon            1..387
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    225..227
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /note="upstream in-frame stop codon"
     exon            388..511
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     CDS             390..1553
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /EC_number="2.4.3.9"
                     /note="isoform b is encoded by transcript variant 2;
                     sialyltransferase 9 (CMP-NeuAc:lactosylceramide
                     alpha-2,3-sialyltransferase); ST3GalV; ganglioside GM3
                     synthase; mST3Gal V; GM3-specific sialytransferase"
                     /codon_start=1
                     /product="lactosylceramide alpha-2,3-sialyltransferase
                     isoform b"
                     /protein_id="NP_035505.2"
                     /db_xref="CCDS:CCDS39510.1"
                     /db_xref="GeneID:20454"
                     /db_xref="MGI:MGI:1339963"
                     /translation="

MPSEFTSAKLRSDCSRTSLQWYTRTQHKMRRPSLLIKDICKCTLVAFGVWLLYILILNYTAEECDMKRMHYVDPDRIKRAQSYAQEVLQKECRPRYAKTAMALLFEDRYSINLEPFVQKVPTASEAELKYDPPFGFRKFSSKVQSLLDMLPEHDFPEHLRAKACKRCVVVGNGGILHGLELGHALNQFDVVIRLNSAPVEGYSEHVGNKTTIRMTYPEGAPLSDVEYYANDLFVTVLFKSVDFKWLQAMVKNESLPFWVRLFFWKQVAEKVPLQPKHFRILNPVIIKETAFDILQYSEPQSRFWGHDKNIPTIGVIAVVLATHLCDEVSLAGFGYDLSQPRTPLHYFDSQCMGAMHWQVMHNVTTETKFLLKLLKEGVVEDLSGGIH"

     misc_feature    774..1523
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /note="Glycosyltransferase family 29 (sialyltransferase);
                     Region: Glyco_transf_29; pfam00777"
                     /db_xref="CDD:425864"
     exon            512..623
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     exon            624..967
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1154
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1313
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..2446
                     /gene="St3gal5"
                     /gene_synonym="3S-T; Siat9; [a]2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ggactgtccggggctgaattggcgcgagcgcggcgccgggggctggctggggcgcggggccccgggctggcggcttgccagcgctccctccctagcatgcacacagaggcggtgggcggcgcggcgcggaggccccagaagctgcgaagccaagcagcggcacctgcctgccgaggtacgtggcgcagcccccagtcggcagagcggacaccggggagcccagatagcggcgatccgcctttgcgataccccaggcccagcggctcctccccagccctgcgacgccggacgcgcctgctaggggacacgggcggagggtcgcggcccctggctgcctacatgggcgcccccggcgcagcctgcgcaggtgtggacgcggcgctgcggcaatgccaagtgagttcacctctgcaaagctgagaagtgattgctcaaggacctccctgcaatggtacacccgaacccagcacaagatgagaagacccagcttgttaataaaagacatctgcaagtgcacgttggttgcatttggagtctggctcctgtacatcctcattttgaattacaccgctgaagaatgtgacatgaaaagaatgcactatgtggaccctgaccggataaagagagctcagagctatgctcaggaagtcttgcagaaggaatgtcggcccaggtacgcgaagacggctatggctctgttatttgaggacaggtacagcatcaacttggagccttttgtgcagaaggtccccacggccagtgaagctgagctcaagtatgacccgccttttggattccggaagttctccagtaaagtccagagcctcttggatatgctgcccgaacatgactttcctgaacacttgagagccaaggcctgcaagcgctgtgtggttgttgggaacgggggcatcctgcacggactagagctgggtcacgccctcaaccagttcgatgtggtaataaggttgaacagtgcgccagttgagggttactctgaacacgttgggaataaaactactataaggatgacttacccagagggtgcgccactgtcggacgttgaatactacgccaatgatttgttcgttactgttttatttaagagtgttgatttcaagtggcttcaagcaatggtaaaaaatgaaagcctgcccttttgggttcgcctcttcttttggaagcaagtggcagaaaaagtcccactccagccaaagcacttcaggattttgaacccagttatcatcaaagaaactgccttcgacatccttcagtactcagagcctcagtcaagattctggggccatgataagaacatccccacgatcggcgtcattgccgttgtcttggctacacatctgtgtgatgaagtcagcctggcaggctttggctacgacctcagtcaacccaggacccctctgcactactttgacagtcagtgcatgggcgccatgcactggcaggtcatgcacaatgtgaccacagagaccaagttcctcctgaagctcctcaaggagggcgtggtggaggacctcagcggcggcatccactgagaactcggaacacggcaaacctcacccagcaccgcagctgagagcgtggtgagcagcctccacagggacttcaccctgcagctgcttcgatgtgcagctagtgttttcaaactccacattttttttaaaaaaggaaaagaaagaacaacagcaacaacaaaagctctgctctgtgcacctcttcgtcctatttatttgaagtcagtgttggattttgcacagttttgtaagttaatcttaagaatgggattggaaggacttttcaaagagaattgtatagtttattgttttttaaggaagtaatttaatttgcagaaactgtacacacgtactctgctcaggtgttgaggtgggaggagaggggcttctggcccctggatgatggctgtgatgcccgatactggggtctgctgctctgtttggtagaactgatggcagagaaacttcctgcctccaggataaagggcttactcatcacctctggcagctgctagacaagttcataacccctttctgctagtccatctgccagctggctcgcaggactcaggcagggcagctgtcccggaggctgctggttggtgagccactgtcagctgagcgccgtgatgttgccccagggtggaagaagccacacttcctacactgtcagggcacttttaaacttctggaggggtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgttcattctgcccttccaaatcatctaagtgttatttaaggcactctgctgtttgtatgagatggttcatagaaattatgacaaagcctttgttatccaggccatgggaagaggaaaaagaaaagaaagaaagaaaagaataaaagcttttgaggagcccctgtga

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