LOCUS       NM_001411786            3441 bp    mRNA    linear   ROD 01-MAY-2025
DEFINITION  Mus musculus tRNA splicing endonuclease subunit 2 (Tsen2),
            transcript variant 4, mRNA.
ACCESSION   NM_001411786
VERSION     NM_001411786.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Markiewicz-Potoczny,M., Lobanova,A., Loeb,A.M., Kirak,O.,
            Olbrich,T., Ruiz,S. and Lazzerini Denchi,E.
  TITLE     TRF2-mediated telomere protection is dispensable in pluripotent
            stem cells
  JOURNAL   Nature 589 (7840), 110-115 (2021)
   PUBMED   33239785
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Bopp,S.E., Ramachandran,V., Henson,K., Luzader,A., Lindstrom,M.,
            Spooner,M., Steffy,B.M., Suzuki,O., Janse,C., Waters,A.P., Zhou,Y.,
            Wiltshire,T. and Winzeler,E.A.
  TITLE     Genome wide analysis of inbred mouse lines identifies a locus
            containing Ppar-gamma as contributing to enhanced malaria survival
  JOURNAL   PLoS One 5 (5), e10903 (2010)
   PUBMED   20531941
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153828.8.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3983994.1,
                                           SRR1660815.449936.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC153828.8         165415-165452
            39-247              AC153828.8         168546-168754
            248-284             AC153828.8         174715-174751
            285-792             AC153828.8         180317-180824
            793-870             AC153828.8         182104-182181
            871-921             AC153828.8         186601-186651
            922-3441            AC153828.8         189420-191939
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.58 cM"
     gene            1..3441
                     /gene="Tsen2"
                     /note="tRNA splicing endonuclease subunit 2"
                     /db_xref="GeneID:381802"
                     /db_xref="MGI:MGI:2141599"
     exon            1..38
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            39..247
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    193..195
                     /gene="Tsen2"
                     /note="upstream in-frame stop codon"
     exon            248..284
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..1068
                     /gene="Tsen2"
                     /EC_number="4.6.1.16"
                     /note="isoform 4 is encoded by transcript variant 4;
                     tRNA-splicing endonuclease subunit Sen2; tRNA-intron
                     endonuclease Sen2; tRNA splicing endonuclease 2 homolog"
                     /codon_start=1
                     /product="tRNA-splicing endonuclease subunit Sen2 isoform
                     4"
                     /protein_id="NP_001398715.1"
                     /db_xref="GeneID:381802"
                     /db_xref="MGI:MGI:2141599"
                     /translation="

MPIITSEKYQHRVEWARDFLRRQGHDESTVQKILTDYTEPLELPCREEKEETPQHEPLSSKADSSLEGRVEKDELPVTPGGAGQSDDLPGLGTHSDCLQEGPGHATLAAASPSSHNGHVAEDPEVLPQETLVPQGGLWPEASSQAAGEKRAAHEYVLIEEELCGAQEEEAAAASDEKLLKRKKLVCRRNPYRIFEYLQLSLEEAFFLAYALGCLSIYYEKEPLTIVKLWQAFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLRE"

     misc_feature    <850..>1062
                     /gene="Tsen2"
                     /note="tRNA-splicing endonuclease subunit sen54 N-term;
                     Region: tRNA_int_end_N2; cl46333"
                     /db_xref="CDD:480673"
     exon            285..792
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            793..870
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            871..921
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     exon            922..3441
                     /gene="Tsen2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2939..2944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsen2"
                     /note="hexamer: AATAAA"
     polyA_site      2978
                     /gene="Tsen2"
     regulatory      3420..3425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Tsen2"
                     /note="hexamer: AATAAA"
     polyA_site      3441
                     /gene="Tsen2"
                     /note="major polyA site"
ORIGIN      

ggttgccgggtaacggtgggcgcgcgtggcggcgaaagagacctgttatccttgaagaatggcagaggcagtcttccgtgctcctaagaggaaaagaagagtctatgagagttacgagtcaccactgcccatcccctttggtcaggaccaaggtcccaggaaggaattccgaatattccaggctgaaatgattagcaacaatgtggtggtgaggggcacagaggacatggagcagctgtatgggaaggtgtgcagacagacatgcctataatcacctcagagaagtatcagcatcgggtggaatgggccagggacttcctgcgtaggcagggccacgatgagagcactgtgcagaagatccttacagactacacagagccactggagcttccttgcagagaggagaaagaagagaccccacagcacgagccactgagctccaaggccgactcctccctggaaggcagagtggaaaaggatgaactccccgtgacccctggaggtgctggacagtcagatgacctacccggtctcggcacacactcagactgcctgcaggaggggcctggccatgccacactcgctgcggctagtccatctagccataatgggcatgtggcagaggaccccgaggttctcccccaggaaaccctcgttccacagggtggcctttggcctgaagccagcagccaggccgcaggagagaaaagggctgctcacgagtatgtgctcatagaggaagagctgtgtggtgcccaggaggaggaggcggcggcggcatcagacgagaagttgctgaaaaggaagaaacttgtgtgcagaagaaatccatacaggatctttgagtatctgcagctcagcctagaagaggctttcttcctggcatacgccctgggatgcctaagtatttactatgagaaggagcctctgacaatagtgaaactctggcaagccttcactgcagtccagcccaccttcaggacaacctacatggcctaccactacttccgaagtaaaggctgggtgcccaaagtggggctcaagtatggaacagatctgcgtgagtaactcttgagtgtgtgtgtgtggcagatgtgaggacagggtcagcccagtttgcttgggaggttgaggtagctttctgttctgttggataccattgctgggtgtttggatgggatgggttaaatccagagaatgagaatgccgtgtgttgggaaagcctactggcaggtgcagactttcctaatcatagcctatgttctatggcacctgctgcacatcagccataagcttgtgtgatttctgtcaggtctgttttcttgcagtttcacttgcctcagactcggagtcctggagttatgatgatagtcagctgttgtttactgagctcatgctgtccggcatggtgttgttcattcctgtgacactcctcatggcagccttttgctattctgtatgccactctcgttccttactgcagtgtagagaggaaaggccatgtaccctcactggcttccagtctctctcaggctttggattctagcacaaggctcttaggcacttctgtgcgttgctggctcctgcagtgatcccctcagactgtgaaaggcttgctgggcagagcaaacagcacagaggctggggcaggacatactgagcaggtctcaggacatacacagaggcaagaatatggagagcactggtcccaaggtactcaggctgggacctggtccaggatgtttgagatgaaaagtcttcagagtgctatcttacaagtgactgaagagaaccaaaatggagaagaaacttgctggtgaagtgggcctgacaacccaccaacagaatggatgcggcacttgtgcacagaggaaccaggtggcatctgatgtcacctcagattcttgatgaattcatctgatgtagtacagactgagagtactaatctgaaatgcttggccacagaagcagtctgggtttcaggcacttataggatgaggtctgcagtgagaattctgaaatagtgctttcattttaaccccagataggaggatatagggctgcttcagactgtccacagcagctgactatgatttgcctcatgctgtagcagaggtgtgattttgccaactacatatagtttttgcagttgtgtgacatatggaattctgggaacttgggcatataaatactagaaccccaagaaatggagttggtggttggtagttgttgttgagtgggtggggagggttggttgcagtttgttagtagtcctgctcaaagaaggaacaaaaggaaagaaattagattcaaggatctctctctctctccccctcctctctatcctttttctcctatccactgataatagggggtaaaaccagtattggggggataaagggtagaaaaaataagaacccacaaagtagcctggttttttgttttttgtttttgtttttcgagacagggtttctctgtatagccctgactgtcctggaactcactctgtagaccaagctggcctcgaactcagaaacccacctatctctgcctcccaagtgctgggattaaaggcgtgcaccaccactgcccagcttagtagcctgttcttaatactcacctttttaattgttttatgcacaagtatatcttcatactgtggaagtttctactgtgacatatcggcactcagcaagcttggattggggcagagggggagcttcagccttgcatgcctgaggctgcacatgatccccaacgaggaagaattcagatcttggagcctcagagattagtgatgctcttcatgattgaaggaggagacaggcagttctggggagctgaagcaccatggcaactctggggagctgtaaatgtcaggggcctgggttagtaaccatctacctttgggttcccctcagctctggggtcattccttaataaatcaccaaaccccatgtccacatgggccatatgaatgagtgcttcctgggtcttttcagaagtatttgtgaggctcagctaaaaattttaaggtatttgagagcctccaaaagttatggcttttgaggtgttaagcaaccttgttgcagcttgttccaaatgtctctaagcctttttaaataacccaaagaaagtcaatgcagtttttaaaattcctacactggggctggagagatgcctcaggttcagggcactggctgctacacttccagaggacctgagtttgattcccagcacccacaaggtggataacaaccgtctgtaactccagtttcagggaatgttacattcctgtctagcctctgttggacactgagcacacatgtggtgcacagaaatgcatgcaggcagaacacacacacacactcacactctcacacacacatagtcatacactcacaattagaaaaacaatgaataaaaactttatgctctaaa

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