LOCUS       NM_001378879            7284 bp    mRNA    linear   ROD 13-MAY-2024
DEFINITION  Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript
            variant 3, mRNA.
ACCESSION   NM_001378879 XM_011240520
VERSION     NM_001378879.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Liu,Q., Pepin,R.M., Novak,M.K., Maschhoff,K.R., Worner,K. and Hu,W.
  TITLE     AGO1 controls protein folding in mouse embryonic stem cell fate
            decisions
  JOURNAL   Dev Cell 59 (8), 979-990 (2024)
   PUBMED   38458189
  REMARK    GeneRIF: AGO1 controls protein folding in mouse embryonic stem cell
            fate decisions.
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 7284)
  AUTHORS   Muller,M., Fah,T., Schaefer,M., Hermes,V., Luitz,J., Stalder,P.,
            Arora,R., Ngondo,R.P. and Ciaudo,C.
  TITLE     AGO1 regulates pericentromeric regions in mouse embryonic stem
            cells
  JOURNAL   Life Sci Alliance 5 (6), e202101277 (2022)
   PUBMED   35236760
  REMARK    GeneRIF: AGO1 regulates pericentromeric regions in mouse embryonic
            stem cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7284)
  AUTHORS   Zhang,Y., Tan,Y.Y., Chen,P.P., Xu,H., Xie,S.J., Xu,S.J., Li,B.,
            Li,J.H., Liu,S., Yang,J.H., Zhou,H. and Qu,L.H.
  TITLE     Genome-wide identification of microRNA targets reveals positive
            regulation of the Hippo pathway by miR-122 during liver development
  JOURNAL   Cell Death Dis 12 (12), 1161 (2021)
   PUBMED   34907157
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7284)
  AUTHORS   Eswarappa,S.M., Potdar,A.A., Koch,W.J., Fan,Y., Vasu,K.,
            Lindner,D., Willard,B., Graham,L.M., DiCorleto,P.E. and Fox,P.L.
  TITLE     Programmed translational readthrough generates antiangiogenic
            VEGF-Ax
  JOURNAL   Cell 157 (7), 1605-1618 (2014)
   PUBMED   24949972
REFERENCE   6  (bases 1 to 7284)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7284)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
REFERENCE   8  (bases 1 to 7284)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   9  (bases 1 to 7284)
  AUTHORS   Cikaluk,D.E., Tahbaz,N., Hendricks,L.C., DiMattia,G.E., Hansen,D.,
            Pilgrim,D. and Hobman,T.C.
  TITLE     GERp95, a membrane-associated protein that belongs to a family of
            proteins involved in stem cell differentiation
  JOURNAL   Mol Biol Cell 10 (10), 3357-3372 (1999)
   PUBMED   10512872
  REMARK    GeneRIF: describes cloning rat sequence and used RNA interference
            to show that the GERp95 orthologue in C. elegans is important for
            maturation of germ-line stem cells in the gonad.
REFERENCE   10 (bases 1 to 7284)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL606935.8 and AL606976.25.
            
            On Mar 12, 2020 this sequence version replaced XM_011240520.2.
            
            Summary: This gene encodes a member of the argonaute family of
            proteins, which associate with small RNAs and have important roles
            in RNA interference (RNAi) and RNA silencing. This protein binds to
            microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses
            translation of mRNAs that are complementary to them. It is also
            involved in transcriptional gene silencing (TGS) of promoter
            regions that are complementary to bound short antigene RNAs
            (agRNAs), as well as in the degradation of miRNA-bound mRNA
            targets. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. A recent study
            showed this gene to be an authentic stop codon readthrough target,
            and that its mRNA could give rise to an additional C-terminally
            extended isoform by use of an alternative in-frame translation
            termination codon. [provided by RefSeq, Nov 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422597.417971.1,
                                           SRR7652917.668935.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-235               AL606935.8         17850-18084         c
            236-419             AL606935.8         13125-13308         c
            420-540             AL606935.8         11138-11258         c
            541-722             AL606935.8         10359-10540         c
            723-859             AL606935.8         9820-9956           c
            860-994             AL606935.8         9454-9588           c
            995-1082            AL606935.8         9233-9320           c
            1083-1230           AL606935.8         8562-8709           c
            1231-1350           AL606935.8         3758-3877           c
            1351-1482           AL606935.8         3270-3401           c
            1483-1616           AL606935.8         3011-3144           c
            1617-1801           AL606976.25        165685-165869       c
            1802-1961           AL606976.25        159999-160158       c
            1962-2052           AL606976.25        159734-159824       c
            2053-2247           AL606976.25        158807-159001       c
            2248-2382           AL606976.25        158095-158229       c
            2383-2484           AL606976.25        157282-157383       c
            2485-2684           AL606976.25        156760-156959       c
            2685-7284           AL606976.25        151930-156529       c
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 60.76 cM"
     gene            1..7284
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="argonaute RISC catalytic subunit 1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
     exon            1..235
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..2793
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     Piwi/Argonaute family protein meIF2C1; argonaute 1;
                     protein argonaute-1; mAgo1; eIF2C 1; eIF-2C 1; argonaute1;
                     eukaryotic translation initiation factor 2C, 1; argonaute
                     RISC catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1 isoform 3"
                     /protein_id="NP_001365808.1"
                     /db_xref="GeneID:236511"
                     /db_xref="MGI:MGI:2446630"
                     /translation="

MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    310..702
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    730..882
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    883..1245
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1039..1041,1084..1086,1126..1128,1138..1140,
                     1192..1194,1213..1215,1219..1221)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1135..1152
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1378..2664
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1783..1911
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1798..1800,1810..1812,1846..1857,1864..1866,
                     1888..1890,1897..1899,1909..1911,1921..1923)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2002..2004,2008..2010,2218..2220,2632..2634)
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2227..2244
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Impairs access of bound RNA to the active site.
                     /evidence=ECO:0000250"
     misc_feature    2341..2355
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2470..2496
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2581..2658
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CJG1.2);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     exon            236..419
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..540
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..722
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..859
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..994
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1082
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1230
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1350
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1482
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1616
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1801
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1961
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2052
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2247
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2382
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2484
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2684
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..7284
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7254..7259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="hexamer: TATAAA"
     polyA_site      7284
                     /gene="Ago1"
                     /gene_synonym="Eif2c1"
                     /note="major polyA site"
ORIGIN      

ggcagctactcgggcgctagcagggggagctgctgcaggctccgcggcggcggcaacggaggctgcgggggcgacagcgcgagcggccgggcttcgggggggagccgagcccggcccgggagcccgagcagtgcaagtgcgaggtacctaggcccctcacgctggactcctcagtctcccggccgcctgtcctccgcacgggtatatgggatggaagcgggaccctcgggagcagcggcaggtgcctacctgcctcccctgcagcaggtgtttcaggcaccccgccggcctggcattggcactgtgggcaaaccaatcaagcttctggccaattactttgaggtggacattcctaagattgacgtttaccattacgaggtggacatcaagccggataagtgtcctcgcagagtcaaccgggaggtggtggaatacatggtccagcatttcaaaccgcagatctttggggatcgcaagcctgtgtatgatggaaagaagaatatttacacggtcacagcactgcccattggcaatgagagggttgactttgaggtgacaatccccggggaggggaaggatagaatttttaaggtctccatcaagtggctagccattgtgagctggcgcatgctgcatgaagccttggtcagtggccagatccctgtgcccctggagtctgtgcaagccctggatgtggccatgaggcacctggcatctatgaggtacacccctgtgggccgctccttcttctcaccgcctgagggctactaccacccgctggggggtgggcgcgaggtctggttcggctttcaccagtctgtgcgccctgccatgtggaagatgatgctcaacattgatgtctcagccactgccttctacaaagcacagccagtgattgagttcatgtgtgaggtcctggacatcaggaacatagacgaacaacccaagcccctcacggattcccagcgtgttcggtttaccaaggagataaaaggcctgaaggtggaagtgacccactgtggacagatgaagaggaaataccgcgtgtgtaatgttacccgccgccctgctagccatcagacgttccccttgcagctggagagtggacagactgtggagtgcactgtggcacagtatttcaagcagaaatataaccttcagctcaagtatcctcacctgccctgcctacaagttgggcaagaacagaagcatacctatttgcccctcgaggtctgtaacattgtggctgggcagcggtgcattaagaagctgactgacaaccagacttcaaccatgataaaggctacagctaggtcggccccagacagacaggaggagatcagccgcctgatgaagaatgctagctacaacctggatccctacatccaggaatttggaatcaaagtgaaggatgacatgacggaggtgacaggacgtgtgctgccggcgcccatcttgcagtatggcggccgggtgagcaggaaccgggccattgctacgcccaaccaaggtgtctgggacatgcgcgggaaacagttctacaatgggattgagatcaaagtctgggccatcgcctgcttcgcaccccagaaacagtgtcgagaagaggtgctcaagaacttcacagaccagcttcggaagatttccaaggatgcagggatgcccatccagggtcagccatgcttctgcaaatacgcacagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactcaggactgcagctcattatcgtcatcctgcccgggaagacgccagtgtatgctgaagtgaaacgtgtcggagatacactcttgggaatggcaacacagtgtgtgcaggtgaagaatgtggtcaagacgtcaccgcagactctgtccaacctctgcctcaagatcaatgtcaaactcggtggcattaacaacatcctagtcccacaccagcgctctgctgtttttcaacagccagtgattttcctgggagcagatgttacacatcccccagctggggatgggaagaaaccatctatcacagcagtggtaggcagcatggacgcacaccccagccgatactgtgccaccgtgcgtgtgcagcgcccacggcaggagatcattgaagacttgtcctatatggtgcgcgagctgcttatccagttctacaagtccacccgcttcaagcccacccgcatcatcttctaccgcgacggcgtccccgagggccagctgccccagattcttcactatgagctgcttgccattcgagatgcatgcatcaaactggaaaaagactaccagcctgggatcacatatattgtggtgcagaagcgccatcacacccgcctgttctgtgctgacaagaatgagcggattgggaagagtggtaacatcccagcagggaccactgtggacaccaacatcacccacccatttgagttcgacttctatctgtgcagccatgcaggcatccagggtaccagccgaccatcccattattatgtcctttgggatgacaaccgtttcacagcagatgaactccagatcttgacataccagctgtgccacacttacgtacgatgcacacgttctgtctctatcccagcacctgcctactatgctcgcttggtggctttccgggcacgataccacctggtggacaaggaacatgacagtggagaggggagccacatatcagggcaaagcaatgggcgagacccccaggccctggccaaagctgtgcaggttcaccaggatactctacgcaccatgtacttcgcttgaaggcagaacgctgttacctcactggatagaagaaagctttccaagccctgggagctgtaccacccaaatccagaggaagcaaggcagagggaggtggggtcggaaggagtataggaggccttgtttctatctacagagggggtataagggtgggaagaggaccagcaagacagactaccagccagaaatcactgatagcaacctcattgtcctcaacccctctccccatcttgtcatatccgacccccttggaccaaaaggggcatcactagtacccaccatacacacagttgtctcatgtgactcacagtgctaaagactcatgctcaacagcctgctagggtcagctctgcagccctgtggtcaaatgctggtatgtttgggtttgctattctagatgagaaagtgggtgggaagagggcaggaatttttaggagccttaatcagaaaaggtctagatttgtttaagaaaaaggaaaacagactcagatcaatattttaggatactagatgttttcatgggttcagaatccactttgtaggaagatttttaatgctaatggtttgggttgctcctccccagctgccatcccctcccatcattcctctccacattctctaccccatgcaccttttccctgacagacatccagccctcgtaacacttaaggcactatggcacttagctctgaggtgacatgaccctgtcttccttccgcctgctggtgggtaaccagtgccttcccgcaacggtaatgctgcagaactgcaaccttttgtacctttctctgtggggtggggtgtgggtgggaaaggagggagggagggagggcagaaatagcccaatgccagcgagcctccagccagaatgccagctaattttcatttgaaggaattgacttccataatcattgagcttttaaaaaaaggtcacaacctcaagatggttaaaatccattgacatttgcactttcagacgtgacaagtctccgagctgctgagatgactggcccttggcctttcccctttagcttcctcttttccctggccccaccaccaccctgccagatctagagttactgtcatagtatttctcattcttggcttttttacccctgatggtaaagtctcgtttcaatatttcttaactggggtgtcttacagcagaagatcgtaaggactaaaataagaaaacaaaaaatctaaaggttatttttataccataacttgagtctattgccaaaatctggaaattccctcccatacctgatgagtctgcattccagaaacatggagccgtaagagggaactgtggaactgacttgtgttctggcctagctaagtagggggtggttgtctccccaaggtggggctcagcctctgtccttcctctgtgcagacaaagccttgttctcgagacagcctccctccagggcgctgtcctgccctcttccctgtaagtgcatcttccccttttctggactggactgtcctttgatccctgactaaccgagactctagcacgtcctctggacaggctggggaattttgctgctcccatttgcttctgtttacacaaatgaaattttcctgtttctcactagggcatgtgagtgggtggcatgggcttttctttttctacccctctcttgaggttctggggttcagctagctgtcttgcaggactggaaaagaagtgggttctagctttgtctttgctgaccttgaagcaaggccccctctttccttgaccattcttttttcttcctgcattattgcttgggatgggaaatttgaacctccgtgtacaggttccccttctgaggagcctgagcttaggattgccctactctggcgatgaagccatgatatgttagctctagcccaggcttagaggcccgctggagaaaggaagatctgaaccctggcctatggagtcaaaacctttccccaagagcccatgtatagacccagatttgctatgcaaatagtccagattcagttatggctggctacattattcagtaacttcccaacaggtagcacaaatattccttatggaaaaagcccaggactgttcagtagttcctcctgtacttttctgctggctacagtatggagtgccccatgggcacaggcccagccggagaacagaacggagggctctgggaagaggcagctcactggagagcctacattccttacacaagtgcctaaacagagtgatgccaacactccatctgccctgtccatcgccttcatagacagtctacttcgtgttccatcaccctttggggagggagctctttgcagacagtgtgttgtcaacttgggtacatttgggggcttggattaagggcactactccaggagggtccaatcattcaccagcatttgcagatgcccatagggaacaggtggctgtcacaactcccagcaacaagattttgttttctcctttttctttgcctcactctctccagttgggttttaagctagaatgaaaagcagtttcggtcctttggtatttatgtgccattcatgaaataaaagcaagagaatgctgggcacgatggcccatgtctgtaattctgttatctcagcactaaggctgaagcagaagatttgcaagttcagggtccacctgggctactcagtgaattccaggccagtgtgcactatgtagcaagaccatgtctccataaaaaagaccaagaaaaatgacttgggtgacagatcttgctgtgattttaaataattctttgagacttaactactgaccatgtgggttcttctcatttaaaggcagtatgcacacattacaaacagtgttgccaagcctcagcttgtttggatttggtttaagtggtgcattgtaccgagctctgggaggattccagcctcatcttagacagctctttctcctcccccaactccctcccatgcctccaccccacccccactgttcggctttccctttcctcttctggccttccctggcttccttttccttctccctcttccctcagcttgcttgctggctggcctgcctgcctgtctgcctgtgtgataatgaattctctgcatggctgcaatgatcccactgttagttggcaaggtcaggcttagctcattgactacagaagaccaaaatcctgtttctcatgcccagtggtgttagccctgggttgcactgtcctcaagcttacactaggtcagagcagcaggcctgtcaaactgacaaaactagtagggtttctggcaggttcaacacctctccattctgtggaggggcttggagaagaggcagtgaaggagaccgctgctttgaattcagtccagtgtgaccgattggttgaggtgctattggcagcctgttggggtcggcctggagctactgttactgggaacttaaacttggactttgactaaagttgaacagagtatgaaactgttctagaaatctagttctcaactgcgacatagctcctctgtgactgagcagcaaggctttccaacaggttccttttaaccagaattctatgaatgatgccggggacacagttttcttagctggcccatagaaggtctacaaggctgctaagtgttgtagacctggcatctggcatcacataggaggatggaatttcttccctttcaggatagaagagtcacctcttacctagccaaaaggcagttttgggaattcctgtcaggaggaaaggtttggttttgttttttaatttactgggctttagatggtagttcattatgccttccttgggcagggatcatggcagggatacgcaaggcactctaatactcggccttctaagcaggctgggaaagggggattctctggttttctctttctccccacttactaggacttgccttctccctgagccacgagagaggctgggttggtgctctccccttactctgctcctactgacttagaacctctggctgctgtatgatagcccaggaaagggaatggaaagaatgggctcaaaacaataatgtaaggaaggtggtttccttccttaaaaagggaaatcagaaactctccaagaattgtgtaagtaaagacaatgtgtctgaatgcactgagtcctctggtgtcatagcaataaggaaaaatgaattgactttctgtgcatacagtccagcctgactggtgtatgatgttgcacttagcagccatgtggtgggcatgtgtgactactctgggtttcactttattttctaaactttttattcctctcaagtccagcatggatggggaaatgtccctggagccccgcagctgtgtacttgtttgcatttgtttcccattgagacttgtgtttgtgtcctgctttgagctgtaccctgtcccgtccattgtgaaattatcccagcagctgtaatgtacagttcccttctcaagcaagcaacagcagcacaatcttgtgttttataaaaacaacagtggcttttatttctaaa

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