LOCUS       NM_001377383            3562 bp    mRNA    linear   ROD 21-SEP-2023
DEFINITION  Mus musculus nrde-2 necessary for RNA interference, domain
            containing (Nrde2), transcript variant 3, mRNA.
ACCESSION   NM_001377383
VERSION     NM_001377383.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Flemr M, Schwaiger M, Hess D, Iesmantavicius V, Ahel J, Tuck AC,
            Mohn F and Buhler M.
  TITLE     Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice
            sites
  JOURNAL   RNA 29 (8), 1140-1165 (2023)
   PUBMED   37137667
  REMARK    GeneRIF: Mouse nuclear RNAi-defective 2 promotes splicing of weak
            5' splice sites.
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco
            G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T,
            Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T,
            Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ,
            Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A,
            Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso
            J, Hide W and Ko MS.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   3  (bases 1 to 3562)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166349.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.144453.1,
                                           ERR3363658.3019714.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               AC166349.4         130627-130736       c
            111-234             AC166349.4         122298-122421       c
            235-468             AC166349.4         120904-121137       c
            469-919             AC166349.4         114826-115276       c
            920-1325            AC166349.4         112980-113385       c
            1326-1459           AC166349.4         111092-111225       c
            1460-1580           AC166349.4         108745-108865       c
            1581-1756           AC166349.4         105444-105619       c
            1757-2143           AC166349.4         103238-103624       c
            2144-3069           AC166349.4         101445-102370       c
            3070-3208           AC166349.4         100382-100520       c
            3209-3280           AC166349.4         97288-97359         c
            3281-3562           AC166349.4         96537-96818         c
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12"
     gene            1..3562
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="nrde-2 necessary for RNA interference, domain
                     containing"
                     /db_xref="GeneID:217827"
                     /db_xref="MGI:MGI:2670969"
     exon            1..110
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..3406
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="isoform c is encoded by transcript variant 3;
                     UPF0614 protein C14orf102 homolog; protein NRDE2 homolog;
                     nuclear exosome regulator NRDE2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform c"
                     /protein_id="NP_001364312.1"
                     /db_xref="GeneID:217827"
                     /db_xref="MGI:MGI:2670969"
                     /translation="

MALFPAFADVSEASGDGAFRKELDWLSNPSFRVGNLTSLSRQTEEVTALASEGSPPPRYSFIRSPLKSELSGESNTSEKLAQTSRKKKKEKKKRRKHQHHRKTKRRHEQLSSSGSESDTEAGKDRASRSIRDDQKEAEKPWYKRKGDSCLGINPKKQCISWEGASAAKKHSHRHLERYFTKKNVGLMRTEGIAVCSNPEPASSEPVTFIPVKDSAEAATPVTSWLNPLGIYDQSTTQWLQGQGPAEQESKQPDSQQDRENAALKARVEEFNRRVRENPWDTQLWMAFVAFQDEVMRSPGIYALGEGEQEKHRKSLKLLLEKKLAVLERAIESNPGSVELKLAKLQLCSEFWEPSALAKEWQKLLFLHPNNTSLWQRYLSFCQSQFGTFSVSKLHSLYGKCLSTLSAVKDGSMLSHPVLPGTEEAMFGLFLQQCHFLRQAGHSEKVISLFQAMVDFTFFKPDSVKELPTKVQVEFFEPFWDSGEPRVGEKGARGWRAWMHQQERGGWVLITPDEDDEEPEEEDQEIKDKTLPRWQIWLAVERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSPDLQFQLIQAFLQFLGVPSGFLPPASCLYLAMDESSIFESELYDEKPLTYFNPSFSGISCVGSMEQLGRPRWTKGHNREGEEFVRNVFHLVLPLLAGKQKSQLSLSWLRYEIAKVIWCLHTKKKRLKSQGKSCKKLAKNLLKEPENRNNFCLWKQYAHLEWLLGNTEDARKVFDTALSMAGSSELKDRELCELSLLYAELEMELSPDSRGATTGRAVHILTRLTESSPYGPYTGQVSSTQVLKARKAYELALQDCLGQSCASSPAPAEALDCLGSLVRCFMLFQYLTVGIDAAVQIYGRVFAKLKGSARLEDPGPEDSTSSQSLTNVLEAVSMMHTSLLRFHMNVCVYPLAPLRETLSDALKLYPGNQVLWRAYVQIQNKSHSANKTRRFFDTVTRSAKHLEPWLFAIEAEKLRKKLVESVQRVGGREVHATIPETGLTHRIRALFENAIRSDKGNQCPLLWRMYLNFLVSLGNKERSKGVFYKALQSCPWAKVLYMDAMEYFPDELQEILDVMTEKELRVRLPLEELELLLED"

     misc_feature    <470..685
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    851..1849
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:429988"
     exon            111..234
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            235..468
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            469..919
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1325
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1459
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1580
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1756
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..2143
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..3069
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3070..3208
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3209..3280
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3281..3562
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agacggcgccatttctgaggcaggagtggtagtgcggcctgtgatcatggcgctgttcccggcttttgcggacgtaagtgaggcctccggtgatggggcattcaggaaagagttagattggctgagcaacccaagctttcgtgttggaaatctaacttctctgagccgacaaactgaagaggtcacagcccttgcttctgaagggtcaccaccgccaaggtactcttttatcaggagtcctctgaagtcagagctctcaggtgagagcaacactagcgagaagctcgcacagacaagcaggaagaagaagaaggagaaaaagaagagacggaagcatcagcaccacaggaaaaccaagaggaggcacgagcagttgagcagcagcggctctgagtcagacacagaggctgggaaggacagggcttcccggagcatcagggacgaccagaaggaagccgagaaaccctggtacaagaggaagggagactcctgcctgggtatcaatcctaagaaacagtgtatatcttgggaaggggcttctgcagcaaagaagcactcacacaggcacctggagcgctattttaccaagaagaatgtggggttaatgagaacagagggcattgctgtgtgcagtaaccccgagcctgcctcctccgagcccgtcacattcatcccagtgaaggactctgctgaggcagctactcctgttacatcatggctgaatcctctggggatttatgatcagtctaccacacagtggttacaaggacagggtccagcagagcaagagtcaaagcagccggactcgcaacaggacagggagaatgctgctctcaaggccagagtggaagagtttaacagaagggttcgggagaatccttgggacacgcaactatggatggcgtttgtggctttccaggacgaggtcatgaggagtcctggcatctatgcccttggggaaggagaacaggagaagcacaggaagtccctgaagctcctcctggagaagaagctggctgtgctggagcgggccatcgagagcaacccaggcagcgtggagctgaagcttgccaagctgcagctgtgctcagagttctgggagcccagtgcgctggccaaggagtggcagaagctcctcttcctgcaccccaacaacacaagcctgtggcagcgctatctctccttttgccagagccagtttggtaccttttctgtgtccaaactccacagtctgtatggcaagtgcttgagtaccttgtctgctgttaaagatggcagcatgctatcccaccctgtgttgccgggcaccgaggaggccatgtttgggctctttcttcagcagtgccactttctacggcaggctggccactcagagaaggtcatctctttgttccaggccatggttgacttcaccttcttcaagcctgacagtgtgaaagagctgcctactaaagtacaggtggaattctttgagcccttctgggacagcggagaaccacgcgttggggagaagggagcccgaggctggcgggcgtggatgcaccaacaggagcgaggtggctgggtgctcatcaccccagatgaggatgacgaggagcccgaagaggaggaccaggagataaaagacaagaccctgccccggtggcagatctggcttgctgttgagcgttctcgagaccagaggcactggcgtccctggcgccctgataagaccaagaagcaaacggaggaagactgtgaggaccctgagagacaggtgctgtttgatgacattgggcaatctttgatcagactttccagcccagaccttcagttccagctcatccaggcctttctgcagttcctgggcgtgccttccggcttcctgcccccagcctcctgcctttacctggccatggacgagagcagcatctttgagagtgaactttatgatgaaaagcccctgacttacttcaacccttccttttcgggcattagctgtgtcggctccatggaacagttgggtcgccctcgctggaccaaaggtcacaaccgagagggcgaggagtttgtccgcaatgtcttccacctggtgctgcctttgctcgcaggcaagcagaagtcccagctcagcctctcctggttacggtacgagattgcaaaggtcatttggtgtctacacactaaaaagaagcgattgaagtctcagggaaagagctgcaaaaaactagccaagaatctcctcaaggagccagaaaaccgcaacaacttctgcctctggaagcagtatgcacacctggagtggttgctcggtaacacagaggatgccagaaaagtgttcgacacagcactcagcatggcaggcagcagcgagctgaaggaccgtgaactctgcgagctcagtctgctctatgccgagctggagatggaactgtcgccggactcgagaggagccaccacaggccgagcggtccacatattaacacggctgacggagagcagtccctacgggccctacacggggcaggtctcgtccacccaggtgctgaaagctcggaaggcttacgagctcgcgctgcaggactgcctgggccagagctgtgcctccagcccagctcctgcagaggccttggactgcctgggtagcttggtccgatgctttatgctcttccagtatttgactgtgggcattgatgctgctgtgcagatatacggaagggtgtttgccaagctgaagggctctgctcgcttagaagacccagggccagaggacagcaccagctcccagagtttgaccaatgtgctcgaggctgtcagcatgatgcacacaagcctgctgcgattccacatgaacgtttgtgtttaccctctggctccactccgagagaccctctccgatgctttaaagctgtatccgggcaaccaggttctttggagggcttatgtacagattcagaataagtcccacagtgctaacaagaccagaaggttctttgacacagttactaggtctgccaaacatttggagccttggctatttgcaatcgaagctgagaagctgaggaaaaagctagtggaatctgttcagagggtaggaggcagagaggtccatgcaacaatccccgagactggccttacgcatcgcatcagagctttgtttgaaaatgcgattcggagcgacaagggcaaccagtgtcccctactgtggaggatgtatttgaattttttggtttccttaggaaacaaagaaagaagcaaaggcgtgttctacaaggccctccagagctgtccttgggcaaaggtgctatacatggacgccatggagtatttcccagacgagctgcaggagatcctggacgtgatgaccgagaaggagctccgtgtgcgcctgccccttgaggagctggagctgctgctggaggattagtgtgcagccaggcctgggccagcctgcagagcccaaagccgcagctgtgcaggctccactgtcctgctggggttggacgtattgacttacatattatatatgtgatctctatgtacaagtggagtcgtgccagtgataaaccttttgtggttgaca

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