LOCUS       NM_001290303            3725 bp    mRNA    linear   ROD 17-SEP-2023
DEFINITION  Mus musculus nrde-2 necessary for RNA interference, domain
            containing (Nrde2), transcript variant 1, mRNA.
ACCESSION   NM_001290303 XM_006515732
VERSION     NM_001290303.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Flemr M, Schwaiger M, Hess D, Iesmantavicius V, Ahel J, Tuck AC,
            Mohn F and Buhler M.
  TITLE     Mouse nuclear RNAi-defective 2 promotes splicing of weak 5' splice
            sites
  JOURNAL   RNA 29 (8), 1140-1165 (2023)
   PUBMED   37137667
  REMARK    GeneRIF: Mouse nuclear RNAi-defective 2 promotes splicing of weak
            5' splice sites.
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco
            G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T,
            Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T,
            Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ,
            Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A,
            Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso
            J, Hide W and Ko MS.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   3  (bases 1 to 3725)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166349.4, CD546085.1, CN706272.1, BM933283.1, CK619854.1,
            DN174402.1, CN710548.1, BG067060.2 and AW989733.1.
            
            On Mar 12, 2014 this sequence version replaced XM_006515732.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a). The exon combination
            of this variant is inferred based on tiled partial transcript
            alignments.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: The exon combination of this CCDS representation is
            inferred due to a lack of full-length transcript support for this
            gene. It is supported by tiled partial transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.104847.1,
                                           SRR1660821.2909.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC166349.4         130706-130740       c
            36-615              CD546085.1         3-582
            616-966             CN706272.1         246-596
            967-1082            AC166349.4         114826-114941       c
            1083-1271           AC166349.4         113197-113385       c
            1272-1501           BM933283.1         1-230
            1502-1622           AC166349.4         111092-111212       c
            1623-1627           AC166349.4         108861-108865       c
            1628-2246           CK619854.1         1-619
            2247-2651           DN174402.1         242-646
            2652-2971           CN710548.1         167-486
            2972-3516           BG067060.2         207-751             c
            3517-3725           AW989733.1         203-411
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12"
     gene            1..3725
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="nrde-2 necessary for RNA interference, domain
                     containing"
                     /db_xref="GeneID:217827"
                     /db_xref="MGI:MGI:2670969"
     exon            1..114
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..3569
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="isoform a is encoded by transcript variant 1;
                     UPF0614 protein C14orf102 homolog; protein NRDE2 homolog;
                     nuclear exosome regulator NRDE2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform a"
                     /protein_id="NP_001277232.1"
                     /db_xref="CCDS:CCDS70414.1"
                     /db_xref="GeneID:217827"
                     /db_xref="MGI:MGI:2670969"
                     /translation="

MALFPAFADVSEASGDGAFRKELDWLSNPSFRVGNLTSLSRQTEEVTALASEGSPPPRYSFIRSPLKSELSGESNTSEKLAQTSRKKKKEKKKRRKHQHHRKTKRRHEQLSSSGSESDTEAGKDRASRSIRDDQKEAEKPCQGSNAAAAVAAAAGHRSIWLEDIHDLTDVFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGASAAKKHSHRHLERYFTKKNVGLMRTEGIAVCSNPEPASSEPVTFIPVKDSAEAATPVTSWLNPLGIYDQSTTQWLQGQGPAEQESKQPDSQQDRENAALKARVEEFNRRVRENPWDTQLWMAFVAFQDEVMRSPGIYALGEGEQEKHRKSLKLLLEKKLAVLERAIESNPGSVELKLAKLQLCSEFWEPSALAKEWQKLLFLHPNNTSLWQRYLSFCQSQFGTFSVSKLHSLYGKCLSTLSAVKDGSMLSHPVLPGTEEAMFGLFLQQCHFLRQAGHSEKVISLFQAMVDFTFFKPDSVKELPTKVQVEFFEPFWDSGEPRVGEKGARGWRAWMHQQERGGWVLITPDEDDEEPEEEDQEIKDKTLPRWQIWLAVERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSPDLQFQLIQAFLQFLGVPSGFLPPASCLYLAMDESSIFESELYDEKPLTYFNPSFSGISCVGSMEQLGRPRWTKGHNREGEEFVRNVFHLVLPLLAGKQKSQLSLSWLRYEIAKVIWCLHTKKKRLKSQGKSCKKLAKNLLKEPENRNNFCLWKQYAHLEWLLGNTEDARKVFDTALSMAGSSELKDRELCELSLLYAELEMELSPDSRGATTGRAVHILTRLTESSPYGPYTGQVSSTQVLKARKAYELALQDCLGQSCASSPAPAEALDCLGSLVRCFMLFQYLTVGIDAAVQIYGRVFAKLKGSARLEDPGPEDSTSSQSLTNVLEAVSMMHTSLLRFHMNVCVYPLAPLRETLSDALKLYPGNQVLWRAYVQIQNKSHSANKTRRFFDTVTRSAKHLEPWLFAIEAEKLRKKLVESVQRVGGREVHATIPETGLTHRIRALFENAIRSDKGNQCPLLWRMYLNFLVSLGNKERSKGVFYKALQSCPWAKVLYMDAMEYFPDELQEILDVMTEKELRVRLPLEELELLLED"

     misc_feature    54..56
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:Q9H7Z3; propagated from
                     UniProtKB/Swiss-Prot (Q80XC6.3); acetylation site"
     misc_feature    186..482
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    561..875
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: MID/MTR4-interacting domain.
                     /evidence=ECO:0000250|UniProtKB:Q9H7Z3"
     misc_feature    561..848
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(561..584,591..596,606..611,615..617,621..665,
                     672..680,684..692,714..722,732..737,741..746,753..773,
                     798..809,819..848)
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    924..989
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    990..1088
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: HAT 1"
     misc_feature    1014..2012
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:429988"
     misc_feature    1260..1358
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: HAT 2"
     misc_feature    2346..2450
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: HAT 3"
     misc_feature    3006..3104
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: HAT 4"
     misc_feature    3273..3377
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80XC6.3);
                     Region: HAT 5"
     exon            115..238
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            239..472
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            473..631
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            632..1082
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1488
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1622
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1743
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1919
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2306
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..3232
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3233..3371
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3372..3443
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3444..3725
                     /gene="Nrde2"
                     /gene_synonym="6720454P05Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctccagacggcgccatttctgaggcaggagtggtagtgcggcctgtgatcatggcgctgttcccggcttttgcggacgtaagtgaggcctccggtgatggggcattcaggaaagagttagattggctgagcaacccaagctttcgtgttggaaatctaacttctctgagccgacaaactgaagaggtcacagcccttgcttctgaagggtcaccaccgccaaggtactcttttatcaggagtcctctgaagtcagagctctcaggtgagagcaacactagcgagaagctcgcacagacaagcaggaagaagaagaaggagaaaaagaagagacggaagcatcagcaccacaggaaaaccaagaggaggcacgagcagttgagcagcagcggctctgagtcagacacagaggctgggaaggacagggcttcccggagcatcagggacgaccagaaggaagccgagaaaccctgtcagggaagcaatgcggctgctgctgttgctgctgctgctggacatcggtccatttggctggaagatattcacgatctgaccgacgtcttcaggactgataagaagccagatcctgccaactgggagtataaatctctgtatcgtggggatatagcaaggtacaagaggaagggagactcctgcctgggtatcaatcctaagaaacagtgtatatcttgggaaggggcttctgcagcaaagaagcactcacacaggcacctggagcgctattttaccaagaagaatgtggggttaatgagaacagagggcattgctgtgtgcagtaaccccgagcctgcctcctccgagcccgtcacattcatcccagtgaaggactctgctgaggcagctactcctgttacatcatggctgaatcctctggggatttatgatcagtctaccacacagtggttacaaggacagggtccagcagagcaagagtcaaagcagccggactcgcaacaggacagggagaatgctgctctcaaggccagagtggaagagtttaacagaagggttcgggagaatccttgggacacgcaactatggatggcgtttgtggctttccaggacgaggtcatgaggagtcctggcatctatgcccttggggaaggagaacaggagaagcacaggaagtccctgaagctcctcctggagaagaagctggctgtgctggagcgggccatcgagagcaacccaggcagcgtggagctgaagcttgccaagctgcagctgtgctcagagttctgggagcccagtgcgctggccaaggagtggcagaagctcctcttcctgcaccccaacaacacaagcctgtggcagcgctatctctccttttgccagagccagtttggtaccttttctgtgtccaaactccacagtctgtatggcaagtgcttgagtaccttgtctgctgttaaagatggcagcatgctatcccaccctgtgttgccgggcaccgaggaggccatgtttgggctctttcttcagcagtgccactttctacggcaggctggccactcagagaaggtcatctctttgttccaggccatggttgacttcaccttcttcaagcctgacagtgtgaaagagctgcctactaaagtacaggtggaattctttgagcccttctgggacagcggagaaccacgcgttggggagaagggagcccgaggctggcgggcgtggatgcaccaacaggagcgaggtggctgggtgctcatcaccccagatgaggatgacgaggagcccgaagaggaggaccaggagataaaagacaagaccctgccccggtggcagatctggcttgctgttgagcgttctcgagaccagaggcactggcgtccctggcgccctgataagaccaagaagcaaacggaggaagactgtgaggaccctgagagacaggtgctgtttgatgacattgggcaatctttgatcagactttccagcccagaccttcagttccagctcatccaggcctttctgcagttcctgggcgtgccttccggcttcctgcccccagcctcctgcctttacctggccatggacgagagcagcatctttgagagtgaactttatgatgaaaagcccctgacttacttcaacccttccttttcgggcattagctgtgtcggctccatggaacagttgggtcgccctcgctggaccaaaggtcacaaccgagagggcgaggagtttgtccgcaatgtcttccacctggtgctgcctttgctcgcaggcaagcagaagtcccagctcagcctctcctggttacggtacgagattgcaaaggtcatttggtgtctacacactaaaaagaagcgattgaagtctcagggaaagagctgcaaaaaactagccaagaatctcctcaaggagccagaaaaccgcaacaacttctgcctctggaagcagtatgcacacctggagtggttgctcggtaacacagaggatgccagaaaagtgttcgacacagcactcagcatggcaggcagcagcgagctgaaggaccgtgaactctgcgagctcagtctgctctatgccgagctggagatggaactgtcgccggactcgagaggagccaccacaggccgagcggtccacatattaacacggctgacggagagcagtccctacgggccctacacggggcaggtctcgtccacccaggtgctgaaagctcggaaggcttacgagctcgcgctgcaggactgcctgggccagagctgtgcctccagcccagctcctgcagaggccttggactgcctgggtagcttggtccgatgctttatgctcttccagtatttgactgtgggcattgatgctgctgtgcagatatacggaagggtgtttgccaagctgaagggctctgctcgcttagaagacccagggccagaggacagcaccagctcccagagtttgaccaatgtgctcgaggctgtcagcatgatgcacacaagcctgctgcgattccacatgaacgtttgtgtttaccctctggctccactccgagagaccctctccgatgctttaaagctgtatccgggcaaccaggttctttggagggcttatgtacagattcagaataagtcccacagtgctaacaagaccagaaggttctttgacacagttactaggtctgccaaacatttggagccttggctatttgcaatcgaagctgagaagctgaggaaaaagctagtggaatctgttcagagggtaggaggcagagaggtccatgcaacaatccccgagactggccttacgcatcgcatcagagctttgtttgaaaatgcgattcggagcgacaagggcaaccagtgtcccctactgtggaggatgtatttgaattttttggtttccttaggaaacaaagaaagaagcaaaggcgtgttctacaaggccctccagagctgtccttgggcaaaggtgctatacatggacgccatggagtatttcccagacgagctgcaggagatcctggacgtgatgaccgagaaggagctccgtgtgcgcctgccccttgaggagctggagctgctgctggaggattagtgtgcagccaggcctgggccagcctgcagagcccaaagccgcagctgtgcaggctccactgtcctgctggggttggacgtattgacttacatattatatatgtgatctctatgtacaagtggagtcgtgccagtgataaaccttttgtggttgaca

//