LOCUS       NM_001168220            4822 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus lysine rich nucleolar protein 1 (Knop1), transcript
            variant 4, mRNA.
ACCESSION   NM_001168220
VERSION     NM_001168220.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   3  (bases 1 to 4822)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 4822)
  AUTHORS   Valerius,M.T. and McMahon,A.P.
  TITLE     Transcriptional profiling of Wnt4 mutant mouse kidneys identifies
            genes expressed during nephron formation
  JOURNAL   Gene Expr Patterns 8 (5), 297-306 (2008)
   PUBMED   18346943
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138717.2 and AC165086.4.
            
            On Jan 14, 2022 this sequence version replaced NM_001168220.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. This variant (4)
            also lacks an exon in the middle portion of the coding region
            compared to variant 1. The encoded isoform (4) is shorter than
            isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC055941.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-297               AC138717.2         105293-105589       c
            298-749             AC138717.2         102794-103245       c
            750-818             AC138717.2         100526-100594       c
            819-944             AC138717.2         98354-98479         c
            945-4822            AC165086.4         1524-5401
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.66 cM"
     gene            1..4822
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="lysine rich nucleolar protein 1"
                     /db_xref="GeneID:66356"
                     /db_xref="MGI:MGI:1913606"
     exon            1..297
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    234..236
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="upstream in-frame stop codon"
     exon            298..749
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..1256
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="isoform 2 is encoded by transcript variant 4;
                     protein C16orf88 homolog; testis-specific gene 118
                     protein"
                     /codon_start=1
                     /product="lysine-rich nucleolar protein 1 isoform 2"
                     /protein_id="NP_001161692.1"
                     /db_xref="CCDS:CCDS52375.1"
                     /db_xref="GeneID:66356"
                     /db_xref="MGI:MGI:1913606"
                     /translation="

MVSKTQKADLGPQLPEKKKKKKKKKRVVANVSEPETQYSVLNSNDYFIDASPPRATSPSNNVDEVQIPEISLSKRKKKKKSCSTHLEECLGAEPTRARQKKSPSPRRQALEQSAEGLIREKKKKRRKSLSKAASQGSGLKTSPDPKHAKEESDTDLEVVLEKKGNMDETCIDQVRRKALQEEIDRESGKTEASEPKKWTVGLSVKTEASEPKKWTGTQFGQWDTAGFENEEQKLKFLKLMGGFKHLSPSFSRPPSMTIRSNMALDKKSSEMLQQSLQQDYDRAMSWKYSHGAGLGFNSEARKVFYIDRNASKSIKLQD"

     misc_feature    450..452
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    474..476
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    723..725
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    963..1184
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Small acidic protein family; Region: SMAP;
                     pfam15477"
                     /db_xref="CDD:464738"
     exon            750..818
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     exon            819..944
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     exon            945..4822
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1252..1257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: ATTAAA"
     polyA_site      1279
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="major polyA site"
     regulatory      1521..1526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: AATAAA"
     polyA_site      1539
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
     regulatory      4797..4802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: AATAAA"
     polyA_site      4822
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="major polyA site"
ORIGIN      

gacttccggatcgcggctgcgcggctgctcacgcggggacgcttggcctgctagcgaaccaggtgagtgcgggtggtttgtgcggggccccgaggaaggtgctgcacgctgggggagaggaaaacaaagataagtcacccccaactcgcctgcagccctcggtttctctccaagccgtcccgccttggagtaggcatggggatgatcctggctagcaagcagcctgcggttggtgagggcagcacacgctcctctggctcgagtccgtgttttgcaccttgttgactttgagacctataatggtcagcaagacccagaaagcagacctgggcccacaactcccagagaagaagaaaaagaagaagaagaaaaagagagtggttgcaaatgtctcagaaccagagactcaatactcagtcttaaacagtaatgattattttatagacgcttctcctccgagggccacgtccccttctaacaatgtggatgaggtgcagatccccgagatatcactgagcaagagaaagaagaaaaaaaagtcctgtagcactcacttggaggagtgcctgggggctgagcctacacgggcaaggcagaaaaagtcacctagccctagaaggcaggcccttgagcagtcagcagaaggcctcatcagagagaagaaaaagaagaggcggaagtccttatcaaaggctgcctcccaaggctcagggctgaagacctccccagaccccaagcatgccaaggaggagtcagacacagacttagaggtggtactggagaaaaaaggcaacatggatgagacctgcatagaccaggtgagacggaaggccttgcaagaagagattgatcgtgagtcgggcaagacagaggcttcagaacccaaaaagtggacagtgggtttgtcggtcaagacagaggcttcagaacccaaaaagtggacgggaacccagtttggccagtgggatactgccggctttgaaaatgaagaacagaagctaaaattcctgaagcttatgggtggctttaagcatctgtccccgtcattcagccgccctcccagcatgactatcaggtccaacatggccctggacaaaaagtcttcagaaatgctccagcagagtctgcagcaggactatgaccgggccatgagctggaagtacagccacggggctggcctgggctttaactctgaggcccgaaaggtcttctacattgaccggaatgcctccaagtctatcaagttgcaagattaaatattattgtcttgttcttcaaactgcatttcttgatcagttgcatccgtcaagttggcttgaaatagtcacctgcagttattgaggaccaataggaattgctaaagcctcctattttctatgttaaacattccacctccgggtgacagtattttaatagtagctagcagttgttggcacgtggagggtcctgactattttccttgcattgttacactaattatggaatgggtgctgttctcatctctatcaagttcagggataaataaagtaattaaacggcctagggtcaccttccgttaagtgccatcaagagtacatcacccagagtcactcaattatcagtgcagaaagcaagtgatatgccatgacttgcaagtttgttttccttctcccttatcaggaggcaggtgtggtgcagcaggcagacgtggccagtacctctgagctggcttctttccagccgtcctccttccatctgactgctgtgcttggatttgatgctgctgtattggctggatctcaaggcattttggacagtaggtaatcctgcgtatggacccatgacagattctcagcagcatgttttgtgtttgtgttcactagacagaagaccttggacacttgtgtgatgaaatcaaacatataaggggtggagaaaaagatactttgctgcaggcaccataccctcccacccctccctcccaaatcacagcaatcttcatggagctcctgatacagagacccatgtgacatgattttctctcccaccagctattggcagtctggtatcaaggttcagagaaggttactttcctgttttggcctgatttctcacaggcagaaacactgggtggggatggaggtgtttgatgctgcactcagccaggacattcattcatccttccagcaccgtcatcagtgttgatgccttcatgctgctaagaagatgagagacaattcccagcattccagagatgggagaaaaccctctctacaactaaagaaacttccggcagtccctcagggaacctgtgatggcgtcattcagctttgtagtttttcctgtatcagcccccttggtaaggagatgcccctcaggccagccagggagagggtgaggtgtgcagtactgccaaatgggcagcagaggtgttgctgtgccccctaccttgttcctgtcctgtgggcataggccatatgtatggcaaagacctgtttacaccactttcctggaatgaacttggcttctcatcaattcagcctcaagattcagaaaagaccacttccagtcacgtcaatggcagaattctgcatttgagtcaacttccagacaaatctccctcggccttggtgaactctgggaaccatctgcataactccaatagcttgtgttccccacaagtcacattccagcctaagtgtctcagctcatcaaaaacttgcacatggcaatcttacgggtggtagatggttttctatcttatgtcacatatgccttggaagctgtcatcaaagtgtgagagccagatctttttcttagctgttacacatacctcagtgctgaaagacttgattttgtagcttggtacctcagcgttgacttaggtgctccactagcccacctttctctaagaattgaagtctgtagtctatctccaaggcactggaggtagggtcagtgatctaacacaaccttgaacttgactgattctctcaggcatttcttaccagcttggtggcacttaagcaatatccttgtccaacagtcaccaaaatctcatgattcttaacacttgtgcttgagctgatttctgtgctatatctgcttgggcccatgaggccctgccctgaggctacatgtacaaccagactaacctgtgaaaaggctctctcagacacaggtgtccctgctcttagtactacttgggttatggtaggcaggacacacacatacccagcagaagggaaattaaagtcactaatcacatgctgctattattattgttgttgttgtttttttgagacagggtttctttgtgtagccctgaagaccataccatgctagccttaaaagtcacagcgattcacctgtctgcctcctggagtgctaggattaaaggtatacatcactgctgcctggcttccagccaccactggctatcagatagatttgaaatcagatccttgataacctgagcaggttcttttcttcacagttccctaactgatacgtagcagaagatttagtgtggaagacacttgggaaggaaaaggccacaagccaagatcacaggcaacgtacagaagccatgcagcatctctttccaccagagtacccacaaaagaacttagacatggggataccttgacctagcccagagaaaccagtgagttctgaccaacagcagtcaagacagtaaaaccacaggaagtcactgtgcttgtaatttcttaaaggaataagaaccaggaatcactcacagatggacatgaggcatcctgtttttcatttcctaaaagtctctttacatgtccttctcaggggaagtagatgctgtttgtggaggcgggcttcaaagactggactgtcagtaacacttctgcccaggtcagcagactgttgcaggagccccgtggtatgtgtacccagtgtgaggataagccgaggttaacacaagcagtgggaattccccaggccagtgtaagctgcttatgctgttatcctgggcagccttgaggcaggagagtaacgtacaaaggattatttatgaatttgccagttatttttgaaagaattagcatcacagtgaaagtgacataaaataattttagtagaaacagtttggtgaaataaaggccatttgggagtatttgaacagcaccatagtgcattctgggttctggcatgttcacagcaggaggaaaggcattttttagcatctggtttttgtttttgagactggggctcatatatcccagaccagcttcaaacatgtaactggggaccttgatcagatcttgatcctcctgtctctacctcctaactactgccgttctaggtctgcaccttggcactctgtcttatgtggggtcttgtgtctgccaggcaagcattccactgactgagccatatccttcgcctcatctgctcatttttggtttggtttttgttttaagaaattgtatcaattccttgggctggagggtattctcatcagattggccccaaatgtatatacaacagtaaccctgctctttgttgttgttgttttgttttgtttgttttttcgagacagggtttctctgtgtagccctgactgtcctggaactcactctgtagaccaacctgaccttgaactcagaaatccacctgcctctgcctctgcctcccaagtgctgggattaaaggcgtgcaccgccaccacctggctgtaactctgctcttaaactagtaaagacacaagctgtgtgtgtatgtgtgtgtgagacttaataacttctactttctgaggttagtcatgtatagtatttcagataaataaaatccatgcctaaagcacaaa

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