LOCUS       NM_001168218            4587 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus lysine rich nucleolar protein 1 (Knop1), transcript
            variant 2, mRNA.
ACCESSION   NM_001168218
VERSION     NM_001168218.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   3  (bases 1 to 4587)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 4587)
  AUTHORS   Valerius,M.T. and McMahon,A.P.
  TITLE     Transcriptional profiling of Wnt4 mutant mouse kidneys identifies
            genes expressed during nephron formation
  JOURNAL   Gene Expr Patterns 8 (5), 297-306 (2008)
   PUBMED   18346943
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138717.2 and AC165086.4.
            
            On Jan 14, 2022 this sequence version replaced NM_001168218.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the middle portion of the coding region, compared to
            variant 1. This results in a shorter protein (isoform 2), compared
            to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3781686.1,
                                           SRR1660825.145315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                AC138717.2         105528-105589       c
            63-514              AC138717.2         102794-103245       c
            515-583             AC138717.2         100526-100594       c
            584-709             AC138717.2         98354-98479         c
            710-4587            AC165086.4         1524-5401
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.66 cM"
     gene            1..4587
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="lysine rich nucleolar protein 1"
                     /db_xref="GeneID:66356"
                     /db_xref="MGI:MGI:1913606"
     exon            1..62
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     exon            63..514
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1021
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein C16orf88 homolog; testis-specific gene 118
                     protein"
                     /codon_start=1
                     /product="lysine-rich nucleolar protein 1 isoform 2"
                     /protein_id="NP_001161690.2"
                     /db_xref="CCDS:CCDS52377.1"
                     /db_xref="GeneID:66356"
                     /db_xref="MGI:MGI:1913606"
                     /translation="

MVSKTQKADLGPQLPEKKKKKKKKKRVVANVSEPETQYSVLNSNDYFIDASPPRATSPSNNVDEVQIPEISLSKRKKKKKSCSTHLEECLGAEPTRARQKKSPSPRRQALEQSAEGLIREKKKKRRKSLSKAASQGSGLKTSPDPKHAKEESDTDLEVVLEKKGNMDETCIDQVRRKALQEEIDRESGKTEASEPKKWTVGLSVKTEASEPKKWTGTQFGQWDTAGFENEEQKLKFLKLMGGFKHLSPSFSRPPSMTIRSNMALDKKSSEMLQQSLQQDYDRAMSWKYSHGAGLGFNSEARKVFYIDRNASKSIKLQD"

     misc_feature    215..217
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    239..241
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    488..490
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q1ED39; propagated from
                     UniProtKB/Swiss-Prot (Q9Z2Q2.2); phosphorylation site"
     misc_feature    728..949
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="Small acidic protein family; Region: SMAP;
                     pfam15477"
                     /db_xref="CDD:464738"
     exon            515..583
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     exon            584..709
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     exon            710..4587
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1017..1022
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: ATTAAA"
     polyA_site      1044
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="major polyA site"
     regulatory      1286..1291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: AATAAA"
     polyA_site      1304
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
     regulatory      4562..4567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="hexamer: AATAAA"
     polyA_site      4587
                     /gene="Knop1"
                     /gene_synonym="2310008H09Rik; Tsg118"
                     /note="major polyA site"
ORIGIN      

gacttccggatcgcggctgcgcggctgctcacgcggggacgcttggcctgctagcgaaccagtaatggtcagcaagacccagaaagcagacctgggcccacaactcccagagaagaagaaaaagaagaagaagaaaaagagagtggttgcaaatgtctcagaaccagagactcaatactcagtcttaaacagtaatgattattttatagacgcttctcctccgagggccacgtccccttctaacaatgtggatgaggtgcagatccccgagatatcactgagcaagagaaagaagaaaaaaaagtcctgtagcactcacttggaggagtgcctgggggctgagcctacacgggcaaggcagaaaaagtcacctagccctagaaggcaggcccttgagcagtcagcagaaggcctcatcagagagaagaaaaagaagaggcggaagtccttatcaaaggctgcctcccaaggctcagggctgaagacctccccagaccccaagcatgccaaggaggagtcagacacagacttagaggtggtactggagaaaaaaggcaacatggatgagacctgcatagaccaggtgagacggaaggccttgcaagaagagattgatcgtgagtcgggcaagacagaggcttcagaacccaaaaagtggacagtgggtttgtcggtcaagacagaggcttcagaacccaaaaagtggacgggaacccagtttggccagtgggatactgccggctttgaaaatgaagaacagaagctaaaattcctgaagcttatgggtggctttaagcatctgtccccgtcattcagccgccctcccagcatgactatcaggtccaacatggccctggacaaaaagtcttcagaaatgctccagcagagtctgcagcaggactatgaccgggccatgagctggaagtacagccacggggctggcctgggctttaactctgaggcccgaaaggtcttctacattgaccggaatgcctccaagtctatcaagttgcaagattaaatattattgtcttgttcttcaaactgcatttcttgatcagttgcatccgtcaagttggcttgaaatagtcacctgcagttattgaggaccaataggaattgctaaagcctcctattttctatgttaaacattccacctccgggtgacagtattttaatagtagctagcagttgttggcacgtggagggtcctgactattttccttgcattgttacactaattatggaatgggtgctgttctcatctctatcaagttcagggataaataaagtaattaaacggcctagggtcaccttccgttaagtgccatcaagagtacatcacccagagtcactcaattatcagtgcagaaagcaagtgatatgccatgacttgcaagtttgttttccttctcccttatcaggaggcaggtgtggtgcagcaggcagacgtggccagtacctctgagctggcttctttccagccgtcctccttccatctgactgctgtgcttggatttgatgctgctgtattggctggatctcaaggcattttggacagtaggtaatcctgcgtatggacccatgacagattctcagcagcatgttttgtgtttgtgttcactagacagaagaccttggacacttgtgtgatgaaatcaaacatataaggggtggagaaaaagatactttgctgcaggcaccataccctcccacccctccctcccaaatcacagcaatcttcatggagctcctgatacagagacccatgtgacatgattttctctcccaccagctattggcagtctggtatcaaggttcagagaaggttactttcctgttttggcctgatttctcacaggcagaaacactgggtggggatggaggtgtttgatgctgcactcagccaggacattcattcatccttccagcaccgtcatcagtgttgatgccttcatgctgctaagaagatgagagacaattcccagcattccagagatgggagaaaaccctctctacaactaaagaaacttccggcagtccctcagggaacctgtgatggcgtcattcagctttgtagtttttcctgtatcagcccccttggtaaggagatgcccctcaggccagccagggagagggtgaggtgtgcagtactgccaaatgggcagcagaggtgttgctgtgccccctaccttgttcctgtcctgtgggcataggccatatgtatggcaaagacctgtttacaccactttcctggaatgaacttggcttctcatcaattcagcctcaagattcagaaaagaccacttccagtcacgtcaatggcagaattctgcatttgagtcaacttccagacaaatctccctcggccttggtgaactctgggaaccatctgcataactccaatagcttgtgttccccacaagtcacattccagcctaagtgtctcagctcatcaaaaacttgcacatggcaatcttacgggtggtagatggttttctatcttatgtcacatatgccttggaagctgtcatcaaagtgtgagagccagatctttttcttagctgttacacatacctcagtgctgaaagacttgattttgtagcttggtacctcagcgttgacttaggtgctccactagcccacctttctctaagaattgaagtctgtagtctatctccaaggcactggaggtagggtcagtgatctaacacaaccttgaacttgactgattctctcaggcatttcttaccagcttggtggcacttaagcaatatccttgtccaacagtcaccaaaatctcatgattcttaacacttgtgcttgagctgatttctgtgctatatctgcttgggcccatgaggccctgccctgaggctacatgtacaaccagactaacctgtgaaaaggctctctcagacacaggtgtccctgctcttagtactacttgggttatggtaggcaggacacacacatacccagcagaagggaaattaaagtcactaatcacatgctgctattattattgttgttgttgtttttttgagacagggtttctttgtgtagccctgaagaccataccatgctagccttaaaagtcacagcgattcacctgtctgcctcctggagtgctaggattaaaggtatacatcactgctgcctggcttccagccaccactggctatcagatagatttgaaatcagatccttgataacctgagcaggttcttttcttcacagttccctaactgatacgtagcagaagatttagtgtggaagacacttgggaaggaaaaggccacaagccaagatcacaggcaacgtacagaagccatgcagcatctctttccaccagagtacccacaaaagaacttagacatggggataccttgacctagcccagagaaaccagtgagttctgaccaacagcagtcaagacagtaaaaccacaggaagtcactgtgcttgtaatttcttaaaggaataagaaccaggaatcactcacagatggacatgaggcatcctgtttttcatttcctaaaagtctctttacatgtccttctcaggggaagtagatgctgtttgtggaggcgggcttcaaagactggactgtcagtaacacttctgcccaggtcagcagactgttgcaggagccccgtggtatgtgtacccagtgtgaggataagccgaggttaacacaagcagtgggaattccccaggccagtgtaagctgcttatgctgttatcctgggcagccttgaggcaggagagtaacgtacaaaggattatttatgaatttgccagttatttttgaaagaattagcatcacagtgaaagtgacataaaataattttagtagaaacagtttggtgaaataaaggccatttgggagtatttgaacagcaccatagtgcattctgggttctggcatgttcacagcaggaggaaaggcattttttagcatctggtttttgtttttgagactggggctcatatatcccagaccagcttcaaacatgtaactggggaccttgatcagatcttgatcctcctgtctctacctcctaactactgccgttctaggtctgcaccttggcactctgtcttatgtggggtcttgtgtctgccaggcaagcattccactgactgagccatatccttcgcctcatctgctcatttttggtttggtttttgttttaagaaattgtatcaattccttgggctggagggtattctcatcagattggccccaaatgtatatacaacagtaaccctgctctttgttgttgttgttttgttttgtttgttttttcgagacagggtttctctgtgtagccctgactgtcctggaactcactctgtagaccaacctgaccttgaactcagaaatccacctgcctctgcctctgcctcccaagtgctgggattaaaggcgtgcaccgccaccacctggctgtaactctgctcttaaactagtaaagacacaagctgtgtgtgtatgtgtgtgtgagacttaataacttctactttctgaggttagtcatgtatagtatttcagataaataaaatccatgcctaaagcacaaa

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