LOCUS       XM_068539498            6304 bp    mRNA    linear   MAM 19-SEP-2024
DEFINITION  PREDICTED: Eschrichtius robustus argonaute RISC component 4 (AGO4),
            transcript variant X2, mRNA.
ACCESSION   XM_068539498
VERSION     XM_068539498.1
DBLINK      BioProject: PRJNA1160892
KEYWORDS    RefSeq.
SOURCE      Eschrichtius robustus (grey whale)
  ORGANISM  Eschrichtius robustus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha;
            Cetacea; Mysticeti; Eschrichtiidae; Eschrichtius.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090826) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_028021215.1-RS_2024_09
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/18/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Eschrichtius robustus"
                     /mol_type="mRNA"
                     /isolate="mEscRob2"
                     /db_xref="taxon:9764"
                     /chromosome="3"
                     /sex="male"
                     /cell_line="mEscRob2 fibroblast"
                     /tissue_type="Cells grown from a skin dart biopsy of
                     epidermis and blubber"
                     /dev_stage="adult"
                     /geo_loc_name="USA: North Pacific Ocean, near Crescent
                     City, California"
                     /lat_lon="41.8349 N 124.2969 W"
                     /collection_date="2016-03-18"
                     /collected_by="Jeffrey K. Jacobsen"
     gene            1..6304
                     /gene="AGO4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 4
                     Proteins"
                     /db_xref="GeneID:137762093"
     CDS             198..2534
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_068395599.1"
                     /db_xref="GeneID:137762093"
                     /translation="

MEALGPGPPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNRDVVDTMVRHFKMQVFGDRQPGYDGKRNMYTAHPLPIGRERVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQCGRQSRVRTEQRPRSSGLG"

     misc_feature    273..662
                     /gene="AGO4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    693..845
                     /gene="AGO4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    846..1208
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1002..1004,1047..1049,1089..1091,1101..1103,
                     1155..1157,1176..1178,1182..1184)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1347..2474
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1758..1760,1770..1772,1806..1817,1824..1826,
                     1848..1850,1857..1859,1869..1871,1881..1883)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
ORIGIN      

agagccgcgggagccgggtccctgtctccgggccgggcgccgtcgccgccccttgctcagcgcccgcgtctccgcggcgcccccatagcgccaatattccggagatcaagcgttacgcggcggcggcggcggcggcggcggcggggcccggagcgggaggcgccggggaccgcggcgaggcggcccccgccgccgccatggaggcgctgggacccggacctccaaccagcctgtttcagccacctcgtcgtcctggccttggaactgttggaaaaccaattcgactgttagccaatcattttcaggttcagattcctaaaatagatgtgtatcactatgacgtggatattaaaccagaaaaacggcctcgtagagtcaacagggacgtagtagatacaatggtgcgccacttcaagatgcaggtatttggtgatcggcagcctggctatgatggcaaaagaaacatgtacacagcacatccactgccaattggacgggagagggttgatatggaagtgacccttccaggtgagggtaaagaccagacctttaaagtgtctgttcagtgggtatcagttgtgagccttcagttgcttttagaagctttggccgggcacttgaatgaagtcccagatgactcagttcaagcacttgatgttatcacaagacaccttccctccatgaggtacactccagtgggtcgctcctttttctcacctcctgaaggttactaccaccctctgggagggggcagggaggtttggtttggctttcatcagtctgtgagacctgccatgtggaatatgatgctgaatattgatgtatctgcaactgctttctaccgggctcagcctatcattgagttcatgtgtgaggttttagacattcagaacatcaatgaacagaccaaacctctaacagactcccagcgtgtcaagtttaccaaagaaatcagaggtcttaaagttgaggtgacccactgtggacagatgaaacgaaaatatcgagtgtgtaatgtgactagacggccagccagtcatcagacttttcccttacagctagaaaatgggcaagcaatggaatgtacagtagctcaatattttaagcaaaagtatagtctgcaactgaaatacccgcatcttccctgtctccaggtgggacaagaacaaaagcatacatacttgccacttgaggtttgtaatatagtggcaggacaacgatgtataaagaagctcacggacaatcagacttccacgatgatcaaagccacagcaagatctgctcctgacagacaggaagaaatcagtagactggtgaagagtaacagcatggtgggcggacctgatccatatcttaaagaatttggtattgttgtccacaatgaaatgactgagctcacaggcagggtacttccagcaccaatgctgcaatacggaggccggaataaaacggtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtttgggcagttgcttgttttgcgcctcagaaacaatgtagggaagatttactaaagagtttcactgaccagctccgtaaaatctctaaggatgcaggaatgcccatccagggtcagccatgtttttgcaagtatgcacaaggtgcagacagcgtggagcccatgtttaaacacctgaaaatgacatatgtgggcctacagctaatagtggttatcttgcctgggaagacaccagtatatgcggaggtgaaacgtgttggagataccctcctgggtatggctacacagtgtgtccaggtaaaaaatgtagtgaagacctcacctcaaaccctttccaacctttgcctgaagataaatgcaaagctcggaggaattaacaacgtgcttgtacctcatcaaaggccctcggtgttccagcagcctgtcatcttcctgggagcggatgtcacgcaccccccagcaggggatgggaagaagccttccattgctgctgttgttggcagtatggacggccaccccagccggtactgtgccaccgttcgggtgcagacgtcccgccaggagatctcccaggagctcctctatagtcaggaggtaatccaggaccttactaacatggttcgagagctgctgatccagttctacaaatccacacgcttcaaacccactcggatcatctattaccgtggaggggtatccgagggacagatgaaacaggtagcttggccagaactaatagcaattcgaaaggcatgtattagtttggaagaagattaccggccaggaataacctatattgtggtgcagaaaagacatcacacacgactcttctgtgcagataaaacagagagggtggggaaaagtggcaatgtaccagcaggcactaccgtggatagcaccatcacgcatccatctgagtttgacttttacctctgtagtcatgcaggaattcagtgcggaaggcagtcacgtgtcaggacagagcaacggccgagatcctcaggccttggctaaagctgtgcagatccaccatgatacccagcacacaatgtattttgcctgagagtctcagaaaaagaactcaaccagtttggcgccccatgcaggctcaaaatgtttcaaacgcctactgcctctagagagagccaagttgacttcaggtggtcttctaccagcaactcagaatagttgcactgaatctatacttcgcagcactgtctgatgcatcaacaaaattgagccatttttttaaagtaatagatactcattgattatcttttctgatgcactggactgaatttttacctcatcatgcaaaggctaatcagcctgaactttctaaaggactttatgagaaaggtttctatggactattttgctagctgtggtgttcaccgcatccctctagtcttacaagagaagcttattcctttcttctgtattcgtcgagtggggtatatacataaatgggagagaaaaaccaaacaatctacttcagttcagtgtaactatttaattgtgtgggtccataggctttttaatggagatttctgacttcaccactgtaaatgcctttaacgagcattaatttttgaaagttttacagagttttattagttttactactttatctattgatctctacagacttgtgctggtgcaagtacaggctgccaggcaattgcactatatttggctgcagattcagacaggcagttctcttatttggttgacttttgtgaatgtgtaacacatgtattgcatgtcgcggtgacagataacaccgccatggtaaaagtactcatgtttccccctctttgaaaaaaattagcttttagtatttttcagagttttcaaaagtgcccattttatgctgctgtgtggtttgttagatctaaatgatttttaaacttttctcatagcaagttaactttggcaataaacatttttttaaagtccctcgtcttcctccccctgacagtgtagttttatagatgagatttcggtatgatttttatcagctgtttcttatagatcataatctggcaatttctgaaactggtcagaaaaggcatatatttccatgcctttttaaagaattctttttatctgttttatagtcacagttggtgtcctgtcactttgtcttaaaataagctagaaccaggatgcttccttattgaataggctttgccagtccttaaggtaccttgagtgatgctgcgacttgcaaaaacgtaccagcatttaaaactttattccctgggaaccaaggttactcacacagttacttgtagtgcaaattttttgcttctctttcaacctgaagtgtacttggtggggtttccttccttttattaaactaaaaccgcaccgaaaaggttgatttttcttaatagattctggattccgttcacagagagacaggattcctttcttgtccaaacaaagggagttgatctaaagcaagcatcagttaaaatgtgggggaaaaggtattttgtatttagtgtagataatactttgtgaatggacaacagttacatagagtcttgatagttccagccagtgtttcccagcccaaattttactgacttggaattctctgtcctggaacctcaggagtgagagctgaaaggagggataaaaggcccagagccactccatatggctgaggacggtgctgggggaagagatagatgcagtgtggtaaaacggttttgccacctctgtctggaaactgctggtgggggaaacactggatctaacggtgaaggatgttgcttgaactcattagctggtccaaatctcttaaactagccttctcaaaatcccagcaatataaagcactgtttttcttcagtcttttaaacaagcttgtaaatacattgaagtttacatttccatgtgctgttgatcagcggcgccaccagtgtttgctgagactggtctctgagagttttataatgtatatggagtcctaaagctcattaagatacaagtgcagacgtccttgttctcttcatagagtgggatgaaagcaagctctgtaattgaggcacaagataggcaggagtgaaggagatggcggatgttaatttcttaagattttccttttttgcaaatacctcacttggacactacaaatcaggacacgttggtgaggggctggctgctgctttttattcacacttgttcagggagagcgcagaaaatacccttcagcttggccaccctggacgcaagaggaggaaaaacggaagttttctctaggaaaaggatgacttgctggagtccaggatgtgaagaactcttggtggctggctggctggccagtgggcaccccatctgcacttgagtgcctcggacttgcttgttaataatgcacagaagaagcgtcacttctaaccatctcctggcatgtatggagcagggagcaaggcggagtgccaggaagccctgacgtgcttgcagatgggctatacactcgaccttgaactgtagtttgtaagacctcagtcagtcctagtacactggggcaaatcagtgtagtggtggaggaaaagccgggaggcctatttttataggaaaacagtacctttgagggtgtaatgctgccgttttggacaaaaggaaagaaatatagaagaattaggattgttggaaaaaacttgctgagcggtgaatctttaagaggtaattactgcagaataccagttgcagcaatacttactgcaggcaggtctgaatgcatagttttaatttggcttgacaatttgaacacgttaaccccttgggactgactgaatcattatgcatgtgtcaatcaatgggaggtgtgcattctctagaagtaggtgtaggcggtttggttttttttaatgcagcctgtttaggcactgtcgcgtggtgtgaaagatgtaaatgtagcaatgctaaagagttaacaattttgggataaggcacggggacattaaaagagtggtgaagatccaaatgttgaaccaattatgttttgttgttgttgttagctgtagagtgaactagctgcccagcagtctgggcagacagaacatattcattcttactgtaaattctatttgctgcttccaaaggagatgatttacaagcagacatattctatatggtctgtattttaggatctggtgcccagcctatatcagagcttacctacctggctcagctacctacccttcctgtgggaaaaggtaatccactgtttaaccaggctcaccctctctgccctgccctagcaaccctttgtggatgtgctgtaagattttcttgctcaatagcaaggtggtagctctgctttcattttaagaaagtggaggctgagggcattgtatcaatactgcttcaactccaaggatttttccttgtaaaattaaagggaaggtcttgttatcgattaaccattttcttaccccttgctattgacatattcatgctctttctatgtctagtagctgaaaaatgtttgcatttgttcatttgactaatggtgtcatttttgtttctatattgttaggcctgtaatgttctaaaatgtattttattaaatttggactggatgtatgtctctagcaatacgaggtactttctaaactattaagggaggggtggtatccccaagttgagataagatgatggttgtttaaattttgcaatttttttttggtctgcagggatattttgtgttcatgtgtccataaaagaataaattggcattcttgtgccaaacattgtttttcttgtcaatt

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