LOCUS       NM_012730               1674 bp    mRNA    linear   ROD 14-MAY-2024
DEFINITION  Rattus norvegicus cytochrome P450, family 2, subfamily d,
            polypeptide 2 (Cyp2d2), mRNA.
ACCESSION   NM_012730
VERSION     NM_012730.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Iwaki,M., Niwa,T., Nakamura,Y., Kawase,A. and Komura,H.
  TITLE     Relative contribution of rat CYP isoforms responsible for
            stereoselective metabolism of carvedilol
  JOURNAL   J Toxicol Sci 43 (1), 59-63 (2018)
   PUBMED   29415952
  REMARK    GeneRIF: It shows the highest metabolic activities against
            enantiomers of carvedilol.
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Bogacz,A., Bartkowiak-Wieczorek,J., Mikolajczak,P.L.,
            Rakowska-Mrozikiewicz,B., Grzeskowiak,E., Wolski,H., Czerny,B. and
            Mrozikiewicz,P.M.
  TITLE     The influence of soybean extract on the expression level of
            selected drug transporters, transcription factors and cytochrome
            P450 genes encoding phase I drug-metabolizing enzymes
  JOURNAL   Ginekol Pol 85 (5), 348-353 (2014)
   PUBMED   25011215
REFERENCE   3  (bases 1 to 1674)
  AUTHORS   Liu,R., Qin,M., Hang,P., Liu,Y., Zhang,Z. and Liu,G.
  TITLE     Effects of Panax notoginseng saponins on the activities of CYP1A2,
            CYP2C9, CYP2D6 and CYP3A4 in rats in vivo
  JOURNAL   Phytother Res 26 (8), 1113-1118 (2012)
   PUBMED   22162298
  REMARK    GeneRIF: Panax notoginseng saponins had no significant effects on
            the activities of CYP2C9, CYP2D6, and CYP3A4, but it could induce
            the activity of CYP1A2 in rats in vivo.
REFERENCE   4  (bases 1 to 1674)
  AUTHORS   Grondin,M., Hamel,F., Sarhan,F. and Averill-Bates,D.A.
  TITLE     Metabolic activity of cytochrome p450 isoforms in hepatocytes
            cryopreserved with wheat protein extract
  JOURNAL   Drug Metab Dispos 36 (10), 2121-2129 (2008)
   PUBMED   18617602
REFERENCE   5  (bases 1 to 1674)
  AUTHORS   Dickmann,L.J., Tay,S., Senn,T.D., Zhang,H., Visone,A.,
            Unadkat,J.D., Hebert,M.F. and Isoherranen,N.
  TITLE     Changes in maternal liver Cyp2c and Cyp2d expression and activity
            during rat pregnancy
  JOURNAL   Biochem Pharmacol 75 (8), 1677-1687 (2008)
   PUBMED   18342837
REFERENCE   6  (bases 1 to 1674)
  AUTHORS   Wan,J., Imaoka,S., Chow,T., Hiroi,T., Yabusaki,Y. and Funae,Y.
  TITLE     Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae
            and their catalytic specificity
  JOURNAL   Arch Biochem Biophys 348 (2), 383-390 (1997)
   PUBMED   9434752
REFERENCE   7  (bases 1 to 1674)
  AUTHORS   Matsunaga,E., Umeno,M. and Gonzalez,F.J.
  TITLE     The rat P450 IID subfamily: complete sequences of four closely
            linked genes and evidence that gene conversions maintained sequence
            homogeneity at the heme-binding region of the cytochrome P450
            active site
  JOURNAL   J Mol Evol 30 (2), 155-169 (1990)
   PUBMED   2107330
REFERENCE   8  (bases 1 to 1674)
  AUTHORS   Matsunaga,E., Zanger,U.M., Hardwick,J.P., Gelboin,H.V., Meyer,U.A.
            and Gonzalez,F.J.
  TITLE     The CYP2D gene subfamily: analysis of the molecular basis of the
            debrisoquine 4-hydroxylase deficiency in DA rats
  JOURNAL   Biochemistry 28 (18), 7349-7355 (1989)
   PUBMED   2819073
REFERENCE   9  (bases 1 to 1674)
  AUTHORS   Ishida,N., Tawaragi,Y., Inuzuka,C., Sugita,O., Kubota,I.,
            Nakazato,H., Noguchi,T. and Sassa,S.
  TITLE     Four species of cDNAs for cytochrome P450 isozymes immunorelated to
            P450C-M/F encode for members of P450IID subfamily, increasing the
            number of members within the subfamily
  JOURNAL   Biochem Biophys Res Commun 156 (2), 681-688 (1988)
   PUBMED   3190674
REFERENCE   10 (bases 1 to 1674)
  AUTHORS   Gonzalez,F.J., Matsunaga,T., Nagata,K., Meyer,U.A., Nebert,D.W.,
            Pastewka,J., Kozak,C.A., Gillette,J., Gelboin,H.V. and
            Hardwick,J.P.
  TITLE     Debrisoquine 4-hydroxylase: characterization of a new P450 gene
            subfamily, regulation, chromosomal mapping, and molecular analysis
            of the DA rat polymorphism
  JOURNAL   DNA 6 (2), 149-161 (1987)
   PUBMED   3582092
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 20, 2020 this sequence version replaced NM_012730.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M22330.1, AB008423.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760400,
                                           SAMEA5760476 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-289               JAXUCZ010000007.1  115818993-115819281 c
            290-461             JAXUCZ010000007.1  115818202-115818373 c
            462-614             JAXUCZ010000007.1  115817815-115817967 c
            615-775             JAXUCZ010000007.1  115817581-115817741 c
            776-952             JAXUCZ010000007.1  115816729-115816905 c
            953-1094            JAXUCZ010000007.1  115816409-115816550 c
            1095-1282           JAXUCZ010000007.1  115816034-115816221 c
            1283-1424           JAXUCZ010000007.1  115815562-115815703 c
            1425-1674           JAXUCZ010000007.1  115815212-115815461 c
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1674
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="cytochrome P450, family 2, subfamily d, polypeptide
                     2"
                     /db_xref="GeneID:25053"
                     /db_xref="RGD:2471"
     exon            1..289
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    50..52
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="upstream in-frame stop codon"
     CDS             101..1603
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /EC_number="1.14.14.1"
                     /note="debrisoquine 4-hydroxylase; cytochrome P450-DB2;
                     cytochrome P450-CMF2; cytochrome P450, family 2, subfamily
                     d, polypeptide 26; Cytochrome P450, subfamily IID2"
                     /codon_start=1
                     /product="cytochrome P450 2D26"
                     /protein_id="NP_036862.2"
                     /db_xref="GeneID:25053"
                     /db_xref="RGD:2471"
                     /translation="

MGLLIGDDLWAVVIFTAIFLLLVDLVHRHKFWTAHYPPGPVPLPGLGNLLQVDFENMPYSLYKLRSRYGDVFSLQIAWKPVVVINGLKAVRELLVTYGEDTADRPLLPIYNHLGYGNKSKGVVLAPYGPEWREQRRFSVSTLRDFGVGKKSLEQWVTEEAGHLCDTFAKEAEHPFNPSILLSKAVSNVIASLVYARRFEYEDPFFNRMLKTLKESFGEDTGFMAEVLNAIPILLQIPGLPGKVFPKLNSFIALVDKMLIEHKKSWDPAQPPRDMTDAFLAEMQKAKGNPESSFNDENLRLVVIDLFMAGMVTTSTTLSWALLLMILHPDVQRRVHEEIDEVIGQVRRPEMADQARMPFTNAVIHEVQRFADIVPTNIPHMTSRDIKFQGFLIPKGTTLIPNLSSVLKDETVWEKPLRFHPEHFLDAQGNFVKHEAFMPFSAGRRACLGEPLARMELFLFFTCLLQRFSFSVLAGRPRPSTHGVYALPVTPQPYQLCAVAR"

     misc_feature    302..1585
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="cytochrome P450 family 2, subfamily D; Region:
                     CYP2D; cd20663"
                     /db_xref="CDD:410756"
     misc_feature    order(410..412,464..469,491..493,503..505,1013..1015,
                     1022..1027,1034..1039,1046..1048,1199..1201,1229..1231,
                     1235..1237,1304..1306,1412..1420,1430..1432,1436..1444,
                     1451..1453)
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="heme binding site [chemical binding]; other site"
                     /db_xref="CDD:410756"
     misc_feature    order(437..439,467..469,743..748,755..757,839..841,
                     848..853,1007..1012,1019..1024,1034..1036,1556..1558)
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="chemical substrate binding pocket [chemical
                     binding]; other site"
                     /db_xref="CDD:410756"
     misc_feature    845..847
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P10634.2); phosphorylation site"
     exon            290..461
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            462..614
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..775
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            776..952
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1094
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1282
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1424
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1674
                     /gene="Cyp2d2"
                     /gene_synonym="Cyp2d26; CYPIID26; P450-CMF2; P450-DB2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

tttaagggaccaggttcccagtctgttgtaagaggatctttcctgattctaagtgtctggacaagtattccttcaaaagcctgcaagccgtggggcagccatggggctgctgattggagatgacctctgggctgtggtcatattcacagccatcttcttgcttctggtggacctggtgcaccggcacaaattctggactgcccactaccctcccggccctgtgccactccctgggctgggaaacctgctgcaggtggactttgagaacatgccatatagcttatacaagcttcgaagccgctatggtgacgtgttcagcctacagatagcctggaagcctgtagttgtgatcaacgggctgaaggccgtgcgggaactgctggtgacctatggagaggacactgctgaccgccccctactgcccatctataatcacctgggctatgggaacaaatcaaaaggtgtggtccttgcaccttacggacctgagtggcgagagcagagacgattctctgtgtccaccctgcgtgacttcggcgtgggcaagaaatcgctggagcagtgggtgacagaggaggccggccacctctgtgacaccttcgccaaggaggctgaacatcccttcaatcccagcatcctcctgagtaaggctgtgagcaacgtgattgcctcccttgtttatgctcgtcgatttgagtatgaggaccctttcttcaacaggatgctcaaaacattgaaggagagctttggagaggacaccggcttcatggcagaggtgctgaacgcaatcccaattctccttcagattcctgggctgcctggcaaagtcttccccaagctcaattcatttatagccttagtggataagatgttaattgagcacaagaaaagctgggatcctgcccagccgccccgagacatgactgatgccttcctagcagagatgcaaaaggccaaggggaatcctgagagcagcttcaatgatgaaaatctgcgcttggtggtgattgacctgttcatggcggggatggtgaccacctcaaccacactgtcctgggcccttctgctcatgatcctgcatccagatgtgcagcgccgagtccacgaggagattgatgaggtcatagggcaggtgcgtcgtccagagatggcagaccaggcacgaatgccgttcaccaatgctgtcatccatgaggtgcagcgctttgcagacatcgtcccgacaaatataccacatatgacatcccgagacattaaattccaaggcttcctcatccccaaggggacaaccctcatccccaacctgtcctccgtgctgaaggatgagaccgtctgggagaagcccctccgcttccatccagaacacttcctggatgcccagggcaactttgtgaagcatgaggccttcatgccattctcagcaggccgcagagcatgcctgggggagcccctggcccgcatggagctcttcctcttcttcacctgcctcctgcagcgcttcagcttctcggtgctggccggccggccccggcccagcacccatggcgtctacgcattgccagttactccacagccttaccaactctgtgcagtggctcgctagcaggggcaggggctccattcttctccccggtttggtctgattgatgtccaataaacaacttctgtatcttc

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