LOCUS       NM_173443               9749 bp    mRNA    linear   ROD 28-APR-2025
DEFINITION  Mus musculus valosin containing protein (p97)/p47 complex
            interacting protein 1 (Vcpip1), mRNA.
ACCESSION   NM_173443
VERSION     NM_173443.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Huang,J., Zhou,Q., Gao,M., Nowsheen,S., Zhao,F., Kim,W., Zhu,Q.,
            Kojima,Y., Yin,P., Zhang,Y., Guo,G., Tu,X., Deng,M., Luo,K.,
            Qin,B., Machida,Y. and Lou,Z.
  TITLE     Tandem Deubiquitination and Acetylation of SPRTN Promotes
            DNA-Protein Crosslink Repair and Protects against Aging
  JOURNAL   Mol Cell 79 (5), 824-835 (2020)
   PUBMED   32649882
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Tsai,Y.C., Kotiya,A., Kiris,E., Yang,M., Bavari,S., Tessarollo,L.,
            Oyler,G.A. and Weissman,A.M.
  TITLE     Deubiquitinating enzyme VCIP135 dictates the duration of botulinum
            neurotoxin type A intoxication
  JOURNAL   Proc Natl Acad Sci U S A 114 (26), E5158-E5166 (2017)
   PUBMED   28584101
  REMARK    GeneRIF: BoNT/A catalytic light chain binds to, and is a substrate
            for, the ubiquitin ligase HECTD2. However, the light chain evades
            proteasomal degradation by the dominant effect of a
            deubiquitinating enzyme, VCIP135/VCPIP1. This deubiquitinating
            enzyme binds BoNT/A light chain directly.
REFERENCE   3  (bases 1 to 9749)
  AUTHORS   Totsukawa,G., Kaneko,Y., Uchiyama,K., Toh,H., Tamura,K. and
            Kondo,H.
  TITLE     VCIP135 deubiquitinase and its binding protein, WAC, in
            p97ATPase-mediated membrane fusion
  JOURNAL   EMBO J 30 (17), 3581-3593 (2011)
   PUBMED   21811234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9749)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   5  (bases 1 to 9749)
  AUTHORS   Dreveny,I., Kondo,H., Uchiyama,K., Shaw,A., Zhang,X. and
            Freemont,P.S.
  TITLE     Structural basis of the interaction between the AAA ATPase p97/VCP
            and its adaptor protein p47
  JOURNAL   EMBO J 23 (5), 1030-1039 (2004)
   PUBMED   14988733
  REMARK    GeneRIF: that p47 ubiquitin regulatory X domain domains may act as
            general p97/VCP/CDC48 binding modules and that adaptor binding for
            N-ethylmaleimide sensitive factor and p97 might involve different
            binding sites
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC059209.1 and AC158790.5.
            
            On Dec 16, 2014 this sequence version replaced NM_173443.2.
            
            Summary: This gene encodes a deubiquitinating enzyme that interacts
            with valosin containing protein p97 and plays a role in the
            assembly of Golgi apparatus during mitosis. [provided by RefSeq,
            Dec 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059209.1, AK122563.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5179              BC059209.1         1-5179
            5180-9749           AC158790.5         147655-152224       c
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.08 cM"
     gene            1..9749
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="valosin containing protein (p97)/p47 complex
                     interacting protein 1"
                     /db_xref="GeneID:70675"
                     /db_xref="MGI:MGI:1917925"
     exon            1..2933
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    149..151
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="upstream in-frame stop codon"
     CDS             227..3889
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /EC_number="3.4.19.12"
                     /note="deubiquitinating protein VCIP135;
                     valosin-containing protein p97/p47 complex-interacting
                     protein p135; VCP/p47 complex-interacting 135-kDa protein"
                     /codon_start=1
                     /product="deubiquitinating protein VCPIP1"
                     /protein_id="NP_775619.2"
                     /db_xref="CCDS:CCDS14814.1"
                     /db_xref="GeneID:70675"
                     /db_xref="MGI:MGI:1917925"
                     /translation="

MSQPPPPPPLPPPPPPPEAPQTSSSLAAAASPGGLSKRRDRRILSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGRDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPSEWLAPGGKLYNLAKSTHGQLRPDKNYSFPLNNLVCSYDPVKDVLLPDYGLSNLTACNWCHGSSVRRVRGDGSIVYLDGDRTNSRSTGGKCGCGFKHFWEGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDPSLNSNVYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQELESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKPSTTFFELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKGRAEGGPSTAAHSAHTVKQEEIAVTGKLSSKELQEQADKEMYSLCLLATLMGEDVWSYAKGLPHMFQQGGVFYNIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASSHPHIDPRARETLAVRKHNTGTDFSNSSIKTEPPVFTAASSNSELIRIAPGVVTMRDGRQIDPDVVEAQRKKLQEMVSSIQASMDKHLRDQSAEQAPSDLSQRKVEVVSSVRPVNLQTGLPEPFSLTGGTENLNTETTDSHVADVLGAAFATRSKAQKENSMEEPEEMDSQDAETTNTTEPMDHS"

     misc_feature    227..346
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CDG3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    332..829
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="VCIP135 N-terminal; Region: VCIP135_N; pfam19437"
                     /db_xref="CDD:437269"
     misc_feature    713..1303
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="OTU (ovarian tumor) domain of deubiquitinating
                     protein VCIP135; Region: OTU_VCIP135; cd22769"
                     /db_xref="CDD:438606"
     misc_feature    order(839..844,1016..1018,1037..1039,1079..1087,
                     1151..1153,1280..1282,1286..1288)
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]; other site"
                     /db_xref="CDD:438606"
     misc_feature    1445..1447
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); acetylation site"
     misc_feature    2396..2560
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CDG3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    2462..2464
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    2492..2494
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    2510..2512
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    2525..2527
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    2546..2770
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     thioesterase OTU1 and similar proteins; Region: Ubl_OTU1;
                     cd17059"
                     /db_xref="CDD:340579"
     misc_feature    order(2555..2557,2582..2584,2675..2677,2684..2686,
                     2690..2692,2696..2698,2744..2758,2768..2770)
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="VCP interaction site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:340579"
     misc_feature    3188..3250
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CDG3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    3203..3205
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    3215..3217
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    3452..3454
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    3563..3646
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CDG3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    3779..3886
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CDG3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    3812..3814
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     misc_feature    3839..3841
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96JH7; propagated from
                     UniProtKB/Swiss-Prot (Q8CDG3.1); phosphorylation site"
     exon            2934..3020
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..9749
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5154..5159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATACA"
     polyA_site      5179
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="major polyA site"
     polyA_site      5201
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      7940..7945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      7960
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      8416..8421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     polyA_site      8438
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      8864..8869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AGTAAA"
     polyA_site      8886
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      9609..9614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: AATAAA"
     regulatory      9618..9623
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: GATAAA"
     polyA_site      9630
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     polyA_site      9635
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
     regulatory      9723..9728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
                     /note="hexamer: ATTAAA"
     polyA_site      9743
                     /gene="Vcpip1"
                     /gene_synonym="4932442A08; 5730421J18Rik; 5730538E15Rik;
                     mKIAA1850; VCIP135"
ORIGIN      

tgcggggtggggtggtggccgttacgttcggggcaacggctaaggcagtggagaagtgagaagagaaacaacgtccggcgcttccgccttcgcctaggaggaggaggaactggtaggaaaaggaaagccattcgccctgggcctggactagacctcgtcgggccggcggaggtctggctatgtgcctttgagggtcgcttgggacgccgagcgagagccgagagcgatgtctcagccgccgccgcctcctccgctgccgccgccgccgcctccccctgaggctccgcagacttcgtcgtctctggcggcggcggcttctccggggggcctttcgaaacgaagggaccgaagaatcctttccgggagttgcccggatcccaagtgccaggcgcgcctcttcttcccggcctcgggttctgtcagcatcgagtgtaccgagtgcggacagcggcacgagcagcaacagctgctgggagtggaggaggtgaccgacccggacgtagtgctgcacaatctgctgcggaacgcgctactcggagtgacgggggctcccaagaagaacacggaactggtaaaggtgatgggcctctccaactaccactgcaaattattgtcgccaatattggcacgctatggaatggacaaacaaacgggccgggccaagcttctccgggacatgaaccaaggcgaactgttcgattgcgcgttacttggtgaccgggccttcctcatcgaaccagagcacgtcaacacagtgggctatggcaaggaccgctccggaagcctcctgtatttgcacgacacgctcgaggatatcaaacgggctaataaaagtcaagaatgcctcatcccagtgcatgtggatggggatggacactgcctagtgcatgctgtgtctagggctctagtaggccgagagctcttctggcatgctctgagggagaatcttaagcagcactttcagcagcacctagcccgatatcaagccctgtttcatgactttattgatgctgccgagtgggaggacattatcaatgaatgtgaccctctgtttgtgccacctgaaggtgtccccttaggcctgaggaatatccacatattcggccttgccaatgtgttacatcgtcccataattcttttagattccctcagcggcatgagaagctctggtgattattcagccacctttctacctggcctcatacctgcagagaaatgcactgggagagatggtcatttgaacaagccaatctgtattgcgtggagtagctctggtagaaaccattatattcccttggtaggcataaagggggctgccctgcccaaactacccatgaatttgttacctaaagcatggggtgtgcctcaggaccttattaaaaagtacatcaagcttgaggaggatggtggctgtgttattggaggagacagaagtttgcaggataagtacttacttaggctggttgctgctatggaagaagtctttatggacaaacatggtatccatcctagtttggttgctgatgtacatcagtatttctacagaaggactggggtgataggagttcagcctgaggaagttacagcagctgctaaaaaggcagtaatggacaatcgccttcacaagtgtttgctttgtggtgccctttctgaacttcatgtcccttctgagtggttggctccaggagggaaactgtataacttggcaaaaagtactcatgggcagttgaggcctgacaaaaattacagctttcccttaaacaatttggtttgttcatatgatccagtaaaagatgttctgctaccagactatggattgagtaatctaacagcttgtaattggtgccatggctcatctgtgcgaagagtcagaggagatggctctattgtgtatttggatggagacagaactaattctaggtctactggtggcaaatgtggttgtggattcaaacatttttgggaaggtaaagagtatgacaatcttccagaagcttttcctatcactttggagtggggaggaagagtagtcagagaaacagtatattggtttcagtatgaaagcgatccatctttgaatagtaatgtttatgatgttgcaatgaaacttgttaccaagcactttccaggtgaatttgggagtgagatcctagttcagaaagttgtccacactatattgcatcagactgcaaaaaaaaatccggacgattacactcctgtaaatatagatggtgctcatgctcagagagttggagatgtgcaaggacaagaattggagtcgcagctaccaactaaaattattcttactggacagaaaacaaaaactttgcataaggaagaattaaacatgagtaaaactgaaagaactattcaacagaacatcacagaacaagcttctgtgatgcagaaacggaaaacagagaaattaaaacaagagcaaaaggggcagcccagaactgtttctccaagtactattcgtgatgggccatcatctgcacctgccacccccaccaaggctccctactcacctaccacttctaaggagaagaagattcgaataacaactaacgatggacggcagtctatggttacccttaagccttcaacaaccttttttgagcttcaggaaagtatagccagagagttcaacattcctccatatttacagtgtattcgatatggctttcctcctaaagagttaatgccaccccaggcagggatggaaaaggagccagttcctttacagcacggtgacagaattacaatagagatcttaaaaggaagagcagaaggtggtccatccactgctgcacactcagcccacactgtgaaacaagaagagattgctgttactggtaaactgtcatctaaggaacttcaggagcaagctgacaaagaaatgtactccttgtgtcttttagctacattaatgggagaagacgtgtggtcttatgcaaagggacttcctcacatgttccagcagggtggtgtattctacaatattatgaagaaaactatgggcatggctgatggcaaacattgtacttttccacatctacctggcaaaacctttgtttataatgcttctgaagatagactggagttgtgtgtcgatgctgcaggacatttccccattggtcctgatgttgaagatttagttaaagaggctgtaagtcaggtgcgagcagaggctactacaagaagtagggaatcaagcccttcacatgggttattaaaactaggtagtggtggagtagtgaaaaagaaatctgagcaacttcacaatgtaactgcctttcaggggaagggccattctctaggaactgcatctagtcacccgcacattgatcccagagctagggaaactctggctgtgagaaagcataatacagggacagattttagtaatagttccattaaaacagagcctcccgtgttcacagctgcttctagtaatagtgagcttattcgaatagctcctggagtagtaacaatgagagacggtaggcagattgatcctgatgtggttgaggcccagcggaaaaaattgcaggaaatggtttcttctattcaggcttcaatggacaagcacttgcgggatcaaagtgcagagcaagcaccatctgatctttcccaaagaaaagtcgaagttgtgagttctgtgaggcctgtgaatcttcagactggcttgcctgaaccgttttctttaactggtggcactgagaatttgaatactgaaacaactgatagtcatgtagcagatgtactgggagcagcatttgccacaaggtcaaaagcacaaaaagaaaattccatggaggaacctgaagagatggatagtcaagatgctgagacgactaacacaactgagccgatggatcactcgtgatttaatttagaggctaataaaggcagaatgtttattgtgaatatgtaatatttgttggctgggccacataacttgagtagtcattaaaaatcttgtacgtatataattcaaagattatatcttgttattcagtgcatgatagcaagtgtgtgattggcaatagcttttaatatactgctgcccaggctggctctgaattcttgtaaattagtttagatcttttgaaagggtttcttccagatatgtggttcattggaagttctttgtcatttttaattccatgaaagtcttaatttccagacatgaaaattctcttgttaaatgtttggtttctgcacaggccagaattgaagaggaaatgacagtttgtacacattgttttcaaggtagccgtcctacatttcatgagaatgttgatttcaagtttacctgaactaaatgtatcttaaaatacataaaacactttattagattacttataatattatagagacagctattgttggcaaaaatatcttgacagaagagagtagtgatacaacattttcctgatttgagcaaacttgggaagaaatatctaccctagtatgataaaatcagtgtgattcagttagttggtctgagatatggccagttgtgtgctcttgttgataatatgttgccagtattttttaaatcttggccttgtcaagttccacctattatttttaaaatcttacataaaagagaatggcattttgttttagaatatttaaaaataatggtagcagtagttaatttctaattttcagagtatgtcagagtcacaaagagtttatctgtatgaatggttctatagttttgacacagtggtgtgtaagcctgtatatgctaaatgtctctaagcccctatgaaccaaaaatgttttccttttttcttagtgtccttaagattttctttttgtcagcttcacttgagtttggatgcatagtttactccagtagaccattagcatttgcttatattgctttcatgcttatgtagtaaaaacaatgcatattcctgagattggtactgctcttgtattctgttctgtagatttgtatttgtaaatgtgttttcagtccatcttcctttgtttactgaaattaagttgaatttgaatgaacgaatgacaaatgtatgttaatataatctcttagagcattcaaagcccggctatctctgtttgcacatgtaacagatgcagaactgtttgcaattctacaaaattgtatgtcagaaaatacattatcaggtgtttaaaaaaatctcttcctgttcattcgaaaagaatgttgtacttgaactttttctagataagggaggttttttgtttttttctgtgatagtatctttaagtgagtctacatactgaaagtccactttgttgaatgtggaaggaagggagaatctagatgtttttaaagtggagctgagtttaagataatgtacaccaccacacacacctgggttagtgtttatcacatcatactttgaaatattttatctcattcatttatattaaagttccaggaaaatatataactttcatatactaattttgcatgaagtaagatagccaaattcttaacaattagctgtgctttaatgcccccccaacccctccactaaccactattattttggcttagtcagccactgagatactcttctcaattttttttcctggtttaagtacacaaaaactgctcaataagcagaaaatgtactgtgtcaaataaattatataatcaccctgctttaacagatgctcttctgtggcttggtattttaagttaataactggcaggatctcaaagtaggagtatcagaaacaagttttgttttttccagacatggtcttactactatgtagccttggctggcctggagcccactctatagaccaggctggccttaaaaagagatctgcctgcctccgtctcccaagtgttgggactagaaggcatactataccatgcctggctagagactaattttatctctctattaaatacttgaatttcgtgtaaattatgcacatgtgaatgttagaagatggacttaatctctgacaatacttcattgtctctagtgaccgtagtggtatttagggttagaaacttaattgggtataaagaaaagtttgggttctcccttgatagaaaatacagacaaacaaccaaacaaaaaacaggtttgagaattcatcagaattcttgaacatatttcaaatcagttgaatgagattattagtggtttggggtaaattttgagagagtttgttttaaacattatttgtgtattttatgtgagtgtagatgcatttttaccattgtgataccactgtgctatgtcagggcagcacaatagcttgtgatctcaagtaaaaatggttttttagccctagaagtaacataactgtaacttaaagctattcagttctatttgcagtgttcactgaatactgaagtaatactgtaaatatttttcttcagtaatccattttgtaaccataccaagtttgagttgatgacccgaagtccaggcagttaaaggtagtgtatgtccagaaggctggtaatgaacaattgctcaagttgaaggaaacaagacactgccaagtattatgagtagtatacaattagctccaagggaatggtcactcagaaactaaagaacaagcctatctagccatttcatcttccaccccttaaactatgtttcaggtgtttattggaaataattaggaaagagacctgagaaatttagcaaatgctagtatttgaaatgctttataccttcctatagtctttagtttggtttttacaattaagctttactgcttgcagttgagatagcattctggaatgtacttgtgagactagccacaggtcttacagatctttattgccattattttaaggatcatattcattggatattaacactgtcttaaatagggtctcttacctcagggaagataatttgctagcaactcctatattattattgacaggttttggagaactactttgacatatcacatgttagctaccaaatgcaaaaacaagtattatgtagttacctgctgtctgctactgaacatttctctagatatttagggccctattattaagtaacatttaaatacgaaataaacttctaagttctgtcattagatacttcatgttttcactatagtgatactatatttttttaaatttcagaacattaatatagttttgtcctcatttttcaagtcaatttaatttgcccaaacttaaatactcaaatacaaacaaatcttgagaattttatttttcttgcttcccctcccaccacccttgatctcaaagcaagatttttataagattcttataaaatcagagcccttgcaaaattggtttaaattcctctcattttaaacttagattcaaagttacttttttataacataaatagatgcatattctaggttggctagatgttcaactttccaagactataacaaaattgtacaacatagaggctcttcacagttagattttttttttttttcaaacaagaacagctcatgaaaataggccatggtagtaaaatgtgtatttaagtaacgaaggggagataaatcactaatgtcacagagttaattgcaagttctgccattctttgagatgccaagtgtttcaattcatatttaatgatactcaaatcgtggaaattttaacgtttgttccaaagtagcagaaactgtaaaaaaaaaaaaaattgtgaattattgaagaaaataccattacttgtatacctgacatttaaagtttttaattatggagttgacatgtttttgtagtataataatacaatgactattctcttgtaaaagaaaaggaaaaactagtaaagaaaaagttttagttgatgttatttgcttgtatcaattactagtattttaaaatttaaggtattgcatactttacgctagaatgcagaaatccctcctggagcctttatgcctgttgtagagcaataaaggactgaaaagcttatcaatattttcaggtttgtttgactgagtattagtgctaagaattgaagagtctccttttttggagggaaatgctacttagattaaaacaaagccataatagtataaatatttagacaaaaaactaacaagtactttaacttggcagtgaagtagtatagatgctattatctgtaaggcacaagctgttagacattactttctggtttcatggttgtttagtatatattaaaattgagaataaataattggtagcagtataacagtatagtgtttttgagcacacatcactgtacagtatttgaaattttgtattaatagaaaactaaataatatattccccctcaacttcacattggctttttaaaaagtaatgttctttagtttcctctaattagagtttaataccataatgcatgaacaaatagttcagttttataataacatttttagcataataaagttgtttttaaaacttatggccaggcagtggtggcacacgccttttattccagcacttgggaggcagagacgtgcggatttcttagttcgagaccagcctcgtctacagagtgagtaccaggacagccagggctgtacagagaaaccctgtctcgaaaaaggaggaaaaaaatcaaaaacaaacaaaaacgaaaaacaacaacaaaaaaatgcccatacatatgaattacccaggaactgtaatttctttccttaatgcaggttttaatattagatgaaatgaatccaattataaaaaaaaatatgcaaaatttctctagaaattttgggtcatgtaatgcttactaaatatttcaaaatgagatatgttctaattgagaatttatttttgaaaagagtacttgttatgatgaggatatgaagggaaaatatagaaaacagcagtaaagtcctcatagctcaccactcaactgtccactttgatttatttttaagccttagtttatttatttaatatctattggatcacagtattggatcagtggttagaactgccctagactccagactcgggtccaagtcaagcatacaacctactgtgtaacctctgtatcactcccaagaaggatgataatggcatttatcaatagagctgggaggattgatgagttattacctgaacattaaaaccatgtctggtacagtgtaccttcaaatactttctgtggttagtatgcttttaagttgtggtaagagttcacctgccactgacatgcagtacagccttacatgtaggccatgtgtgttatactcttaaatctaaaacttgttgaatgtgtatagtatgttaccaacgagtagaaactggatatttttacatccaaatatttctatgagggcttaatctcacgaactaccaagaacctggagagttggccttctgtgctttgtgattgagatgtgctatggagcctttaaaggcccataaagctgctgtaaccttgaggtttgcagttgtacctctgttctgttgaccataggtacaatgctgagagtagtgctgaagcaacaggcactgtgaactcagaatgctgagactacagtcacaaacttgagcttgaggtcttctgtgtgtgtgcatgccattgtccatataccatcaagatagagctctcaaaacttcagtcaataaagaagataaacattccacttaagatagttcattactggttttagttttattgactggctgtcataccccagtaagtaaaataaggctgaataattgattgtttctaataattaaaaatgtacagagaaaagttgaa

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