LOCUS       NM_023258               2135 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus PYD and CARD domain containing (Pycard), mRNA.
ACCESSION   NM_023258
VERSION     NM_023258.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Saller,B.S., Wohrle,S., Fischer,L., Dufossez,C., Ingerl,I.L.,
            Kessler,S., Mateo-Tortola,M., Gorka,O., Lange,F., Cheng,Y.,
            Neuwirt,E., Marada,A., Koentges,C., Urban,C., Aktories,P.,
            Reuther,P., Giese,S., Kirschnek,S., Mayer,C., Pilic,J.,
            Falquez-Medina,H., Oelgeklaus,A., Deepagan,V.G., Shojaee,F.,
            Zimmermann,J.A., Weber,D., Tai,Y.H., Crois,A., Ciminski,K.,
            Peyronnet,R., Brandenburg,K.S., Wu,G., Baumeister,R.,
            Heimbucher,T., Rizzi,M., Riedel,D., Helmstadter,M., Buescher,J.,
            Neumann,K., Misgeld,T., Kerschensteiner,M., Walentek,P., Kreutz,C.,
            Maurer,U., Rambold,A.S., Vince,J.E., Edlich,F., Malli,R.,
            Hacker,G., Kierdorf,K., Meisinger,C., Kottgen,A., Jakobs,S.,
            Weber,A.N.R., Schwemmle,M., Gross,C.J. and Gross,O.
  TITLE     Acute suppression of mitochondrial ATP production prevents
            apoptosis and provides an essential signal for NLRP3 inflammasome
            activation
  JOURNAL   Immunity 58 (1), 90-107 (2025)
   PUBMED   39571574
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Jena,K.K., Mambu,J., Boehmer,D., Sposito,B., Millet,V., de Sousa
            Casal,J., Muendlein,H.I., Spreafico,R., Fenouil,R., Spinelli,L.,
            Wurbel,S., Riquier,C., Galland,F., Naquet,P., Chasson,L.,
            Elkins,M., Mitsialis,V., Ketelut-Carneiro,N., Bugda Gwilt,K.,
            Thiagarajah,J.R., Ruan,H.B., Lin,Z., Lien,E., Shao,F., Chou,J.,
            Poltorak,A., Ordovas-Montanes,J., Fitzgerald,K.A., Snapper,S.B.,
            Broggi,A. and Zanoni,I.
  TITLE     Type III interferons induce pyroptosis in gut epithelial cells and
            impair mucosal repair
  JOURNAL   Cell 187 (26), 7533-7550 (2024)
   PUBMED   39500322
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   Wang,Y., Zhang,Y., Kim,K., Han,J., Okin,D., Jiang,Z., Yang,L.,
            Subramaniam,A., Means,T.K., Nestle,F.O., Fitzgerald,K.A.,
            Randolph,G.J., Lesser,C.F., Kagan,J.C., Mathis,D. and Benoist,C.
  TITLE     A pan-family screen of nuclear receptors in immunocytes reveals
            ligand-dependent inflammasome control
  JOURNAL   Immunity 57 (12), 2737-2754 (2024)
   PUBMED   39571575
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   Ding,S.Q., Yan,H.Z., Gao,J.X., Chen,Y.Q., Zhang,N., Wang,R.,
            Li,J.Y., Hu,J.G. and Lu,H.Z.
  TITLE     Genetic deletion of the apoptosis associated speck like protein
            containing a card in LysM+ macrophages attenuates spinal cord
            injury by regulating M1/M2 polarization through ASC-dependent
            inflammasome signaling axis
  JOURNAL   Exp Neurol 382, 114982 (2024)
   PUBMED   39353545
  REMARK    GeneRIF: Genetic deletion of the apoptosis associated speck like
            protein containing a card in LysM[+] macrophages attenuates spinal
            cord injury by regulating M1/M2 polarization through ASC-dependent
            inflammasome signaling axis.
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Pizzuto,M., Hurtado-Navarro,L., Molina-Lopez,C., Soubhye,J.,
            Gelbcke,M., Rodriguez-Lopez,S., Ruysschaert,J.M., Schroder,K. and
            Pelegrin,P.
  TITLE     Ornithine lipid is a partial TLR4 agonist and NLRP3 activator
  JOURNAL   Cell Rep 43 (10), 114788 (2024)
   PUBMED   39340778
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   Yamamoto,M., Yaginuma,K., Tsutsui,H., Sagara,J., Guan,X., Seki,E.,
            Yasuda,K., Yamamoto,M., Akira,S., Nakanishi,K., Noda,T. and
            Taniguchi,S.
  TITLE     ASC is essential for LPS-induced activation of procaspase-1
            independently of TLR-associated signal adaptor molecules
  JOURNAL   Genes Cells 9 (11), 1055-1067 (2004)
   PUBMED   15507117
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Mariathasan,S., Newton,K., Monack,D.M., Vucic,D., French,D.M.,
            Lee,W.P., Roose-Girma,M., Erickson,S. and Dixit,V.M.
  TITLE     Differential activation of the inflammasome by caspase-1 adaptors
            ASC and Ipaf
  JOURNAL   Nature 430 (6996), 213-218 (2004)
   PUBMED   15190255
  REMARK    GeneRIF: cell death triggered by stimuli that engage caspase-1 was
            ablated in macrophages lacking either ASC or Ipaf, suggesting a
            coupling between the inflammatory and cell death pathways
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Reed,J.C., Doctor,K., Rojas,A., Zapata,J.M., Stehlik,C.,
            Fiorentino,L., Damiano,J., Roth,W., Matsuzawa,S., Newman,R.,
            Takayama,S., Marusawa,H., Xu,F., Salvesen,G. and Godzik,A.
  CONSRTM   RIKEN GER Group; GSL Members
  TITLE     Comparative analysis of apoptosis and inflammation genes of mice
            and humans
  JOURNAL   Genome Res 13 (6B), 1376-1388 (2003)
   PUBMED   12819136
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Martinon,F., Hofmann,K. and Tschopp,J.
  TITLE     The pyrin domain: a possible member of the death domain-fold family
            implicated in apoptosis and inflammation
  JOURNAL   Curr Biol 11 (4), R118-R120 (2001)
   PUBMED   11250163
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Masumoto,J., Taniguchi,S., Nakayama,K., Ayukawa,K. and Sagara,J.
  TITLE     Murine ortholog of ASC, a CARD-containing protein, self-associates
            and exhibits restricted distribution in developing mouse embryos
  JOURNAL   Exp Cell Res 262 (2), 128-133 (2001)
   PUBMED   11139337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DT921526.1, AK152677.1, CJ137522.1 and BG071362.2.
            
            On Jan 16, 2008 this sequence version replaced NM_023258.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152677.1, AK018682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               DT921526.1         1-141
            142-1405            AK152677.1         2-1265
            1406-1611           CJ137522.1         245-450
            1612-2135           BG071362.2         1-524               c
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.88 cM"
     gene            1..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain containing"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
     exon            1..485
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    182..184
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="upstream in-frame stop codon"
     CDS             215..796
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain-containing protein"
                     /codon_start=1
                     /product="apoptosis-associated speck-like protein
                     containing a CARD"
                     /protein_id="NP_075747.3"
                     /db_xref="CCDS:CCDS21888.1"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
                     /translation="

MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS"

     misc_feature    227..472
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Pyrin Death Domain found in ASC; Region:
                     Pyrin_ASC-like; cd08321"
                     /db_xref="CDD:260033"
     misc_feature    545..787
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Caspase activation and recruitment domain found in
                     Human ASC, NALP1, and similar proteins; Region:
                     CARD_ASC_NALP1; cd08330"
                     /db_xref="CDD:260039"
     misc_feature    791..793
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9EPB4.1); phosphorylation site"
     exon            486..542
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      875
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="major polyA site"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="hexamer: ATTAAA"
     polyA_site      1412
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
ORIGIN      

caaaaagccaagtctgaaagtcttaggggcggaaaccaaaactagtttgctggggaaagaacaggagctgtaagaaaagagggtgggggagtcccagcatgcccatcggcctaagcagctgacttcctggtcttggcgggctggcagcaggcaggctgcagcaggcgagcagcagcaagagtaaaaggtgaccgcggctgcccaccccagagccatggggcgggcacgagatgccatcctggacgctcttgaaaacttgtcaggggatgaactcaaaaagttcaagatgaagctgctgacagtgcaactgcgagaaggctatgggcgcatcccacgcggggccctgctgcagatggacgccatagatctcactgacaaacttgtcagctactatctggagtcgtatggcttggagctcacaatgactgtgcttagagacatgggcttacaggagctggctgagcagctgcaaacgactaaagaagagtctggagctgtggcagctgcagccagtgtccctgctcagagtacagccagaacaggacactttgtggaccagcacaggcaagcactcattgccagggtcacagaagtggacggagtgctggatgctttgcatggcagtgtgctgactgaaggacagtaccaggcagttcgtgcagagaccaccagccaagacaagatgaggaagctcttcagctttgttccatcctggaacctgacctgcaaggactccctcctccaggccttgaaggaaatacatccctacttggtgatggacctggagcagagctgaggtatcttttccagctacattatctagctcctgactttgtatacacaatttttgaaaaaacaatttgtatttgtgtttactctctgggatgtttttgctgagctaagctacatttccaaaggccatatgtggcccagtggtagaacatataccaaacatgcacaaatcagtctccaacacctaaattccacagacccaagttatggcttctccattctagtgcaaaggcttctcaaatgattcctggctccccatttgggagcttttcccatattatcccaaaaggctacctttgagaacacatgtaacattatccaggtcaagctaaaaagcgagagggggggatgggaaccaggtaatggttaatcccagcaaccaggaggcagaggaggctgttccagaacagccaagggtacagagagaaaccctatctcaaaaaaacaaaaacaaaaaaaaaacaggcaatggggcttagaaaggcacctagaaacaaaccagtccgtagaagactttgggctctgggaactcctgtctagacaaggctggcctaactcaagagatctgcccagctctgcctcctgagttatgagattaaatgcatgtatcaccatgcccagcccaagcctttttttttttttaattatgtgtggattttgggggtggggctgttcatatgtgtgcaccatttcatgcaatgccaaccaaagccagaagagggcatcagatcccctggaacaggagttactggctgagcctccacacaatgctgggaatttaacatgggtttcccctggaaaaagcagtcgttacgactgcagaatcatctctccaaccccaagggcttatcctttaaagaaatgaagatcgtattgtgtgaatataaacacgggtgcatgtctgcacagaagcttgaagacaaactccagagttctgtttcttaccttgaggtagggaggagttcctgttctttttgctgcatactaactctgggctgtgagcttcagggccattctgtttctctcatctgtactttcaggtctctcagcagtgctgagattaacaggtattgccatcattcagtggacagaagacaaaaccagggtgaacgaagctctctacaaccgagtttcatccccagtccagtagctactcttccaagtgttatttatggtcctacctatggaattttttgaatctcacttgacccctagatccctgtcttaaataggcctgggatcaaaggcctaacaagttcacgtgaaatcttattagtgattggagaggacaaaaaaggttgccttttattggtatgcacagtctattagaagggtcctttgtg

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