LOCUS       NM_023258               2135 bp    mRNA    linear   ROD 08-JUL-2025
DEFINITION  Mus musculus PYD and CARD domain containing (Pycard), mRNA.
ACCESSION   NM_023258
VERSION     NM_023258.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Chen,Y., Chen,S., Liu,Z., Wang,Y., An,N., Chen,Y., Peng,Y., Liu,Z.,
            Liu,Q. and Hu,X.
  TITLE     Red blood cells undergo lytic programmed cell death involving NLRP3
  JOURNAL   Cell 188 (11), 3013-3029 (2025)
   PUBMED   40252640
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Winsor,N.J., Bayer,G., Singh,O., Chan,J.K., Li,L.Y., Lieng,B.Y.,
            Foerster,E., Popovic,A., Tsankov,B.K., Maughan,H., Lemire,P.,
            Tam,E., Streutker,C., Chen,L., Heaver,S.L., Ley,R.E., Parkinson,J.,
            Montenegro-Burke,J.R., Birchenough,G.M.H., Philpott,D.J. and
            Girardin,S.E.
  TITLE     Cross-kingdom-mediated detection of intestinal protozoa through
            NLRP6
  JOURNAL   Cell Host Microbe 33 (3), 388-407 (2025)
   PUBMED   40043701
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   McManus,R.M., Komes,M.P., Griep,A., Santarelli,F., Schwartz,S.,
            Ramon Perea,J., Schlachetzki,J.C.M., Bouvier,D.S., Khalil,M.A.,
            Lauterbach,M.A., Heinemann,L., Schluter,T., Pour,M.S., Lovotti,M.,
            Stahl,R., Duthie,F., Rodriguez-Alcazar,J.F., Schmidt,S.V.,
            Spitzer,J., Noori,P., Maillo,A., Boettcher,A., Herron,B.,
            McConville,J., Gomez-Cabrero,D., Tegner,J., Glass,C.K., Hiller,K.,
            Latz,E. and Heneka,M.T.
  TITLE     NLRP3-mediated glutaminolysis controls microglial phagocytosis to
            promote Alzheimer's disease progression
  JOURNAL   Immunity 58 (2), 326-343 (2025)
   PUBMED   39904338
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   Alarcon-Vila,C., Hurtado-Navarro,L., Mateo,S.V., Penin-Franch,A.,
            Martinez,C.M., Molina-Lopez,C., Banos,M.C., Gomez,A.I.,
            Gomez-Roman,J., Baroja-Mazo,A., Arostegui,J.I., Palmou-Fontana,N.,
            Martinez-Garcia,J.J. and Pelegrin,P.
  TITLE     The inflammasome adaptor protein ASC promotes amyloid deposition in
            cryopyrin-associated periodic syndromes
  JOURNAL   EMBO Mol Med 17 (1), 41-53 (2025)
   PUBMED   39639164
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Villacampa,N., Sarlus,H., Martorell,P., Bhalla,K., Castro-Gomez,S.,
            Vieira-Saecker,A., Slutzkin,I., Handler,K., Venegas,C., McManus,R.,
            Ulas,T., Beyer,M., Segal,E. and Heneka,M.T.
  TITLE     Proliferating Microglia Exhibit Unique Transcriptional and
            Functional Alterations in Alzheimer's Disease
  JOURNAL   ASN Neuro 17 (1), 2506406 (2025)
   PUBMED   40387894
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   Yamamoto,M., Yaginuma,K., Tsutsui,H., Sagara,J., Guan,X., Seki,E.,
            Yasuda,K., Yamamoto,M., Akira,S., Nakanishi,K., Noda,T. and
            Taniguchi,S.
  TITLE     ASC is essential for LPS-induced activation of procaspase-1
            independently of TLR-associated signal adaptor molecules
  JOURNAL   Genes Cells 9 (11), 1055-1067 (2004)
   PUBMED   15507117
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Mariathasan,S., Newton,K., Monack,D.M., Vucic,D., French,D.M.,
            Lee,W.P., Roose-Girma,M., Erickson,S. and Dixit,V.M.
  TITLE     Differential activation of the inflammasome by caspase-1 adaptors
            ASC and Ipaf
  JOURNAL   Nature 430 (6996), 213-218 (2004)
   PUBMED   15190255
  REMARK    GeneRIF: cell death triggered by stimuli that engage caspase-1 was
            ablated in macrophages lacking either ASC or Ipaf, suggesting a
            coupling between the inflammatory and cell death pathways
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Reed,J.C., Doctor,K., Rojas,A., Zapata,J.M., Stehlik,C.,
            Fiorentino,L., Damiano,J., Roth,W., Matsuzawa,S., Newman,R.,
            Takayama,S., Marusawa,H., Xu,F., Salvesen,G. and Godzik,A.
  CONSRTM   RIKEN GER Group; GSL Members
  TITLE     Comparative analysis of apoptosis and inflammation genes of mice
            and humans
  JOURNAL   Genome Res 13 (6B), 1376-1388 (2003)
   PUBMED   12819136
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Martinon,F., Hofmann,K. and Tschopp,J.
  TITLE     The pyrin domain: a possible member of the death domain-fold family
            implicated in apoptosis and inflammation
  JOURNAL   Curr Biol 11 (4), R118-R120 (2001)
   PUBMED   11250163
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Masumoto,J., Taniguchi,S., Nakayama,K., Ayukawa,K. and Sagara,J.
  TITLE     Murine ortholog of ASC, a CARD-containing protein, self-associates
            and exhibits restricted distribution in developing mouse embryos
  JOURNAL   Exp Cell Res 262 (2), 128-133 (2001)
   PUBMED   11139337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DT921526.1, AK152677.1, CJ137522.1 and BG071362.2.
            
            On Jan 16, 2008 this sequence version replaced NM_023258.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152677.1, AK018682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               DT921526.1         1-141
            142-1405            AK152677.1         2-1265
            1406-1611           CJ137522.1         245-450
            1612-2135           BG071362.2         1-524               c
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.88 cM"
     gene            1..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain containing"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
     exon            1..485
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    182..184
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="upstream in-frame stop codon"
     CDS             215..796
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain-containing protein"
                     /codon_start=1
                     /product="apoptosis-associated speck-like protein
                     containing a CARD"
                     /protein_id="NP_075747.3"
                     /db_xref="CCDS:CCDS21888.1"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
                     /translation="

MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS"

     misc_feature    227..472
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Pyrin Death Domain found in ASC; Region:
                     Pyrin_ASC-like; cd08321"
                     /db_xref="CDD:260033"
     misc_feature    545..787
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Caspase activation and recruitment domain found in
                     Human ASC, NALP1, and similar proteins; Region:
                     CARD_ASC_NALP1; cd08330"
                     /db_xref="CDD:260039"
     misc_feature    791..793
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9EPB4.1); phosphorylation site"
     exon            486..542
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      875
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="major polyA site"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="hexamer: ATTAAA"
     polyA_site      1412
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
ORIGIN      

caaaaagccaagtctgaaagtcttaggggcggaaaccaaaactagtttgctggggaaagaacaggagctgtaagaaaagagggtgggggagtcccagcatgcccatcggcctaagcagctgacttcctggtcttggcgggctggcagcaggcaggctgcagcaggcgagcagcagcaagagtaaaaggtgaccgcggctgcccaccccagagccatggggcgggcacgagatgccatcctggacgctcttgaaaacttgtcaggggatgaactcaaaaagttcaagatgaagctgctgacagtgcaactgcgagaaggctatgggcgcatcccacgcggggccctgctgcagatggacgccatagatctcactgacaaacttgtcagctactatctggagtcgtatggcttggagctcacaatgactgtgcttagagacatgggcttacaggagctggctgagcagctgcaaacgactaaagaagagtctggagctgtggcagctgcagccagtgtccctgctcagagtacagccagaacaggacactttgtggaccagcacaggcaagcactcattgccagggtcacagaagtggacggagtgctggatgctttgcatggcagtgtgctgactgaaggacagtaccaggcagttcgtgcagagaccaccagccaagacaagatgaggaagctcttcagctttgttccatcctggaacctgacctgcaaggactccctcctccaggccttgaaggaaatacatccctacttggtgatggacctggagcagagctgaggtatcttttccagctacattatctagctcctgactttgtatacacaatttttgaaaaaacaatttgtatttgtgtttactctctgggatgtttttgctgagctaagctacatttccaaaggccatatgtggcccagtggtagaacatataccaaacatgcacaaatcagtctccaacacctaaattccacagacccaagttatggcttctccattctagtgcaaaggcttctcaaatgattcctggctccccatttgggagcttttcccatattatcccaaaaggctacctttgagaacacatgtaacattatccaggtcaagctaaaaagcgagagggggggatgggaaccaggtaatggttaatcccagcaaccaggaggcagaggaggctgttccagaacagccaagggtacagagagaaaccctatctcaaaaaaacaaaaacaaaaaaaaaacaggcaatggggcttagaaaggcacctagaaacaaaccagtccgtagaagactttgggctctgggaactcctgtctagacaaggctggcctaactcaagagatctgcccagctctgcctcctgagttatgagattaaatgcatgtatcaccatgcccagcccaagcctttttttttttttaattatgtgtggattttgggggtggggctgttcatatgtgtgcaccatttcatgcaatgccaaccaaagccagaagagggcatcagatcccctggaacaggagttactggctgagcctccacacaatgctgggaatttaacatgggtttcccctggaaaaagcagtcgttacgactgcagaatcatctctccaaccccaagggcttatcctttaaagaaatgaagatcgtattgtgtgaatataaacacgggtgcatgtctgcacagaagcttgaagacaaactccagagttctgtttcttaccttgaggtagggaggagttcctgttctttttgctgcatactaactctgggctgtgagcttcagggccattctgtttctctcatctgtactttcaggtctctcagcagtgctgagattaacaggtattgccatcattcagtggacagaagacaaaaccagggtgaacgaagctctctacaaccgagtttcatccccagtccagtagctactcttccaagtgttatttatggtcctacctatggaattttttgaatctcacttgacccctagatccctgtcttaaataggcctgggatcaaaggcctaacaagttcacgtgaaatcttattagtgattggagaggacaaaaaaggttgccttttattggtatgcacagtctattagaagggtcctttgtg

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