LOCUS       NM_023258               2135 bp    mRNA    linear   ROD 23-SEP-2025
DEFINITION  Mus musculus PYD and CARD domain containing (Pycard), mRNA.
ACCESSION   NM_023258
VERSION     NM_023258.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Castanheira,F.V.S., Pereira,M.S.F., Ataide,M.A.,
            Mascarenhas,D.P.A., Guerra,R.O., Quirino,G.F.S., Almeida,F. and
            Zamboni,D.S.
  TITLE     Bacterial type IV secretion system induces specific and nonspecific
            protective immunity
  JOURNAL   mBio 16 (8), e0044825 (2025)
   PUBMED   40558085
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Chen,Y., Chen,S., Liu,Z., Wang,Y., An,N., Chen,Y., Peng,Y., Liu,Z.,
            Liu,Q. and Hu,X.
  TITLE     Red blood cells undergo lytic programmed cell death involving NLRP3
  JOURNAL   Cell 188 (11), 3013-3029 (2025)
   PUBMED   40252640
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   Winsor,N.J., Bayer,G., Singh,O., Chan,J.K., Li,L.Y., Lieng,B.Y.,
            Foerster,E., Popovic,A., Tsankov,B.K., Maughan,H., Lemire,P.,
            Tam,E., Streutker,C., Chen,L., Heaver,S.L., Ley,R.E., Parkinson,J.,
            Montenegro-Burke,J.R., Birchenough,G.M.H., Philpott,D.J. and
            Girardin,S.E.
  TITLE     Cross-kingdom-mediated detection of intestinal protozoa through
            NLRP6
  JOURNAL   Cell Host Microbe 33 (3), 388-407 (2025)
   PUBMED   40043701
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   McManus,R.M., Komes,M.P., Griep,A., Santarelli,F., Schwartz,S.,
            Ramon Perea,J., Schlachetzki,J.C.M., Bouvier,D.S., Khalil,M.A.,
            Lauterbach,M.A., Heinemann,L., Schluter,T., Pour,M.S., Lovotti,M.,
            Stahl,R., Duthie,F., Rodriguez-Alcazar,J.F., Schmidt,S.V.,
            Spitzer,J., Noori,P., Maillo,A., Boettcher,A., Herron,B.,
            McConville,J., Gomez-Cabrero,D., Tegner,J., Glass,C.K., Hiller,K.,
            Latz,E. and Heneka,M.T.
  TITLE     NLRP3-mediated glutaminolysis controls microglial phagocytosis to
            promote Alzheimer's disease progression
  JOURNAL   Immunity 58 (2), 326-343 (2025)
   PUBMED   39904338
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Villacampa,N., Sarlus,H., Martorell,P., Bhalla,K., Castro-Gomez,S.,
            Vieira-Saecker,A., Slutzkin,I., Handler,K., Venegas,C., McManus,R.,
            Ulas,T., Beyer,M., Segal,E. and Heneka,M.T.
  TITLE     Proliferating Microglia Exhibit Unique Transcriptional and
            Functional Alterations in Alzheimer's Disease
  JOURNAL   ASN Neuro 17 (1), 2506406 (2025)
   PUBMED   40387894
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   Yamamoto,M., Yaginuma,K., Tsutsui,H., Sagara,J., Guan,X., Seki,E.,
            Yasuda,K., Yamamoto,M., Akira,S., Nakanishi,K., Noda,T. and
            Taniguchi,S.
  TITLE     ASC is essential for LPS-induced activation of procaspase-1
            independently of TLR-associated signal adaptor molecules
  JOURNAL   Genes Cells 9 (11), 1055-1067 (2004)
   PUBMED   15507117
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Mariathasan,S., Newton,K., Monack,D.M., Vucic,D., French,D.M.,
            Lee,W.P., Roose-Girma,M., Erickson,S. and Dixit,V.M.
  TITLE     Differential activation of the inflammasome by caspase-1 adaptors
            ASC and Ipaf
  JOURNAL   Nature 430 (6996), 213-218 (2004)
   PUBMED   15190255
  REMARK    GeneRIF: cell death triggered by stimuli that engage caspase-1 was
            ablated in macrophages lacking either ASC or Ipaf, suggesting a
            coupling between the inflammatory and cell death pathways
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Reed,J.C., Doctor,K., Rojas,A., Zapata,J.M., Stehlik,C.,
            Fiorentino,L., Damiano,J., Roth,W., Matsuzawa,S., Newman,R.,
            Takayama,S., Marusawa,H., Xu,F., Salvesen,G. and Godzik,A.
  CONSRTM   RIKEN GER Group; GSL Members
  TITLE     Comparative analysis of apoptosis and inflammation genes of mice
            and humans
  JOURNAL   Genome Res 13 (6B), 1376-1388 (2003)
   PUBMED   12819136
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Martinon,F., Hofmann,K. and Tschopp,J.
  TITLE     The pyrin domain: a possible member of the death domain-fold family
            implicated in apoptosis and inflammation
  JOURNAL   Curr Biol 11 (4), R118-R120 (2001)
   PUBMED   11250163
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Masumoto,J., Taniguchi,S., Nakayama,K., Ayukawa,K. and Sagara,J.
  TITLE     Murine ortholog of ASC, a CARD-containing protein, self-associates
            and exhibits restricted distribution in developing mouse embryos
  JOURNAL   Exp Cell Res 262 (2), 128-133 (2001)
   PUBMED   11139337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DT921526.1, AK152677.1, CJ137522.1 and BG071362.2.
            
            On Jan 16, 2008 this sequence version replaced NM_023258.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152677.1, AK018682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               DT921526.1         1-141
            142-1405            AK152677.1         2-1265
            1406-1611           CJ137522.1         245-450
            1612-2135           BG071362.2         1-524               c
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.88 cM"
     gene            1..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain containing"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
     exon            1..485
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    182..184
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="upstream in-frame stop codon"
     CDS             215..796
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="PYD and CARD domain-containing protein"
                     /codon_start=1
                     /product="apoptosis-associated speck-like protein
                     containing a CARD"
                     /protein_id="NP_075747.3"
                     /db_xref="CCDS:CCDS21888.1"
                     /db_xref="GeneID:66824"
                     /db_xref="MGI:MGI:1931465"
                     /translation="

MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS"

     misc_feature    227..472
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Pyrin Death Domain found in ASC; Region:
                     Pyrin_ASC-like; cd08321"
                     /db_xref="CDD:260033"
     misc_feature    545..787
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Caspase activation and recruitment domain found in
                     Human ASC, NALP1, and similar proteins; Region:
                     CARD_ASC_NALP1; cd08330"
                     /db_xref="CDD:260039"
     misc_feature    791..793
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9EPB4.1); phosphorylation site"
     exon            486..542
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..2135
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      875
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="major polyA site"
     regulatory      1387..1392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
                     /note="hexamer: ATTAAA"
     polyA_site      1412
                     /gene="Pycard"
                     /gene_synonym="9130417A21Rik; Asc; CARD5; masc; TMS-1;
                     TNS1"
ORIGIN      

caaaaagccaagtctgaaagtcttaggggcggaaaccaaaactagtttgctggggaaagaacaggagctgtaagaaaagagggtgggggagtcccagcatgcccatcggcctaagcagctgacttcctggtcttggcgggctggcagcaggcaggctgcagcaggcgagcagcagcaagagtaaaaggtgaccgcggctgcccaccccagagccatggggcgggcacgagatgccatcctggacgctcttgaaaacttgtcaggggatgaactcaaaaagttcaagatgaagctgctgacagtgcaactgcgagaaggctatgggcgcatcccacgcggggccctgctgcagatggacgccatagatctcactgacaaacttgtcagctactatctggagtcgtatggcttggagctcacaatgactgtgcttagagacatgggcttacaggagctggctgagcagctgcaaacgactaaagaagagtctggagctgtggcagctgcagccagtgtccctgctcagagtacagccagaacaggacactttgtggaccagcacaggcaagcactcattgccagggtcacagaagtggacggagtgctggatgctttgcatggcagtgtgctgactgaaggacagtaccaggcagttcgtgcagagaccaccagccaagacaagatgaggaagctcttcagctttgttccatcctggaacctgacctgcaaggactccctcctccaggccttgaaggaaatacatccctacttggtgatggacctggagcagagctgaggtatcttttccagctacattatctagctcctgactttgtatacacaatttttgaaaaaacaatttgtatttgtgtttactctctgggatgtttttgctgagctaagctacatttccaaaggccatatgtggcccagtggtagaacatataccaaacatgcacaaatcagtctccaacacctaaattccacagacccaagttatggcttctccattctagtgcaaaggcttctcaaatgattcctggctccccatttgggagcttttcccatattatcccaaaaggctacctttgagaacacatgtaacattatccaggtcaagctaaaaagcgagagggggggatgggaaccaggtaatggttaatcccagcaaccaggaggcagaggaggctgttccagaacagccaagggtacagagagaaaccctatctcaaaaaaacaaaaacaaaaaaaaaacaggcaatggggcttagaaaggcacctagaaacaaaccagtccgtagaagactttgggctctgggaactcctgtctagacaaggctggcctaactcaagagatctgcccagctctgcctcctgagttatgagattaaatgcatgtatcaccatgcccagcccaagcctttttttttttttaattatgtgtggattttgggggtggggctgttcatatgtgtgcaccatttcatgcaatgccaaccaaagccagaagagggcatcagatcccctggaacaggagttactggctgagcctccacacaatgctgggaatttaacatgggtttcccctggaaaaagcagtcgttacgactgcagaatcatctctccaaccccaagggcttatcctttaaagaaatgaagatcgtattgtgtgaatataaacacgggtgcatgtctgcacagaagcttgaagacaaactccagagttctgtttcttaccttgaggtagggaggagttcctgttctttttgctgcatactaactctgggctgtgagcttcagggccattctgtttctctcatctgtactttcaggtctctcagcagtgctgagattaacaggtattgccatcattcagtggacagaagacaaaaccagggtgaacgaagctctctacaaccgagtttcatccccagtccagtagctactcttccaagtgttatttatggtcctacctatggaattttttgaatctcacttgacccctagatccctgtcttaaataggcctgggatcaaaggcctaacaagttcacgtgaaatcttattagtgattggagaggacaaaaaaggttgccttttattggtatgcacagtctattagaagggtcctttgtg

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