LOCUS       NM_001382483            1698 bp    mRNA    linear   ROD 19-NOV-2023
DEFINITION  Mus musculus AKT1 substrate 1 (Akt1s1), transcript variant 8, mRNA.
ACCESSION   NM_001382483 XM_006541121
VERSION     NM_001382483.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Cai S, Yang Q, Cao Y, Li Y, Liu J, Wang J, Zhang X, Liu L, Li X and
            Zhang Y.
  TITLE     PF4 antagonizes retinal neovascularization via inhibiting PRAS40
            phosphorylation in a mouse model of oxygen-induced retinopathy
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (3), 165604 (2020)
   PUBMED   31740404
  REMARK    GeneRIF: This study, for the first time, links PF4's anti-RNV
            function to an intracellular signaling molecule PRAS40 and its
            phosphorylation.
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Figlia G, Norrmen C, Pereira JA, Gerber D and Suter U.
  TITLE     Dual function of the PI3K-Akt-mTORC1 axis in myelination of the
            peripheral nervous system
  JOURNAL   Elife 6, e29241 (2017)
   PUBMED   28880149
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1698)
  AUTHORS   Yang W, Yang LF, Song ZQ, Shah SZA, Cui YY, Li CS, Zhao HF, Gao HL,
            Zhou XM and Zhao DM.
  TITLE     PRAS40 alleviates neurotoxic prion peptide-induced apoptosis via
            mTOR-AKT signaling
  JOURNAL   CNS Neurosci Ther 23 (5), 416-427 (2017)
   PUBMED   28294542
  REMARK    GeneRIF: PRAS40 inhibits mTORC1 hyperactivation and plays a key
            role in protecting cells against neurotoxic prion peptide-induced
            apoptosis. Thus, PRAS40 is a potential therapeutic target for prion
            disease.
REFERENCE   4  (bases 1 to 1698)
  AUTHORS   Cordon-Barris L, Pascual-Guiral S, Yang S, Gimenez-Llort L,
            Lope-Piedrafita S, Niemeyer C, Claro E, Lizcano JM and Bayascas JR.
  TITLE     Mutation of the 3-Phosphoinositide-Dependent Protein Kinase 1
            (PDK1) Substrate-Docking Site in the Developing Brain Causes
            Microcephaly with Abnormal Brain Morphogenesis Independently of
            Akt, Leading to Impaired Cognition and Disruptive Behaviors
  JOURNAL   Mol Cell Biol 36 (23), 2967-2982 (2016)
   PUBMED   27644329
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1698)
  AUTHORS   Rho O, Srivastava J, Cho J and DiGiovanni J.
  TITLE     Overexpression of PRAS40(T246A) in the Proliferative Compartment
            Suppresses mTORC1 Signaling, Keratinocyte Migration, and Skin Tumor
            Development
  JOURNAL   J Invest Dermatol 136 (10), 2070-2079 (2016)
   PUBMED   27349859
  REMARK    GeneRIF: Overexpression of PRAS40(T246A) in the Proliferative
            Compartment Suppresses mTORC1 Signaling, Keratinocyte Migration,
            and Skin Tumor Development
REFERENCE   6  (bases 1 to 1698)
  AUTHORS   Saito A, Hayashi T, Okuno S, Nishi T and Chan PH.
  TITLE     Modulation of proline-rich akt substrate survival signaling
            pathways by oxidative stress in mouse brains after transient focal
            cerebral ischemia
  JOURNAL   Stroke 37 (2), 513-517 (2006)
   PUBMED   16397181
  REMARK    GeneRIF: overexpression of SOD1 may affect the PRAS pathway after
            transient focal cerebral ischemia by reducing the direct oxidative
            reaction to pPRAS after reperfusion injury
REFERENCE   7  (bases 1 to 1698)
  AUTHORS   Ballif BA, Villen J, Beausoleil SA, Schwartz D and Gygi SP.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 1698)
  AUTHORS   Saito A, Narasimhan P, Hayashi T, Okuno S, Ferrand-Drake M and Chan
            PH.
  TITLE     Neuroprotective role of a proline-rich Akt substrate in apoptotic
            neuronal cell death after stroke: relationships with nerve growth
            factor
  JOURNAL   J Neurosci 24 (7), 1584-1593 (2004)
   PUBMED   14973226
  REMARK    GeneRIF: PRAS phosphorylation and its interaction with pAkt and
            14-3-3 might play an important role in neuroprotection mediated by
            NGF in apoptotic neuronal cell death after transient focal cerebral
            ischemia
REFERENCE   9  (bases 1 to 1698)
  AUTHORS   Kovacina KS, Park GY, Bae SS, Guzzetta AW, Schaefer E, Birnbaum MJ
            and Roth RA.
  TITLE     Identification of a proline-rich Akt substrate as a 14-3-3 binding
            partner
  JOURNAL   J Biol Chem 278 (12), 10189-10194 (2003)
   PUBMED   12524439
  REMARK    GeneRIF: The protein product encoded by 1110012J22 gene is
            proline-rich substrate of Akt which also binds to 14-3-3.  A
            proposed name for this protein is thus PRAS40, for proline-rich
            substrate of Akt of 40 kDa.
REFERENCE   10 (bases 1 to 1698)
  AUTHORS   Chern JJ and Choi KW.
  TITLE     Lobe mediates Notch signaling to control domain-specific growth in
            the Drosophila eye disc
  JOURNAL   Development 129 (17), 4005-4013 (2002)
   PUBMED   12163404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158231.2.
            
            On May 12, 2020 this sequence version replaced XM_006541121.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.4144190.1,
                                           SRR12282455.25411100.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-234               AC158231.2         123071-123304
            235-623             AC158231.2         125270-125658
            624-701             AC158231.2         126479-126556
            702-871             AC158231.2         126638-126807
            872-1698            AC158231.2         127291-128117
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..1698
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="AKT1 substrate 1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
     exon            1..234
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    65..67
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="upstream in-frame stop codon"
     exon            235..623
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..1015
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="isoform b is encoded by transcript variant 8;
                     proline-rich AKT1 substrate 1; proline-rich AKT substrate;
                     AKT1 substrate 1 (proline-rich)"
                     /codon_start=1
                     /product="proline-rich AKT1 substrate 1 isoform b"
                     /protein_id="NP_001369412.1"
                     /db_xref="GeneID:67605"
                     /db_xref="MGI:MGI:1914855"
                     /translation="

MASGRPEELWEAVVGAAERFQARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIAQAHRAATATRPPGPPPAPQPPSPAPSPPPRPALAREDEEEDEDEPTETETSGERLGGSDNGGLFMMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPTACPQPPATALPTQQYAKSLPVSVPVWAFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY"

     misc_feature    392..394
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); methylation site"
     misc_feature    431..784
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D1F4.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    503..505
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    515..517
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    590..592
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    623..997
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Proline-rich AKT1 substrate 1; Region: PRAS;
                     pfam15798"
                     /db_xref="CDD:406279"
     misc_feature    629..643
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D1F4.1);
                     Region: TOS motif. /evidence=ECO:0000250|UniProtKB:Q96B36"
     misc_feature    791..793
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    830..892
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D1F4.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    848..850
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    851..853
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    875..877
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    878..880
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355,
                     ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    905..907
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96B36; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     misc_feature    980..982
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9D1F4.1); phosphorylation site"
     exon            624..701
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            702..871
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1698
                     /gene="Akt1s1"
                     /gene_synonym="1110012J22Rik; Lobe; Lobel; PRAS40"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agagcaggggcctctctgggctgcgggcgtggtgtagccctggtcggcggcctcacttcggtggtaggatccttcttggacgtcccgcaccgtgtcccgtgctgcggccgttttcttttctatctctcttcacaactgtcctgggaggcctgcaccttcccgctggacagctcccccttcctggtttgccccagagtcttgcctcataggaggccataggcctaggaggttggaggctcggatggcgtctgggcggccagaggaactgtgggaagccgtcgtgggggccgccgagcgctttcaggcccgcactggcacagagctggtattactgactgcagcgccaccgccgccgccccgccctggaccctgtgcctatgccgcccatggccgcggagccctggcagaggcggcccgacgctgcctccacgacatcgcacaggcgcacagggctgccactgccacccgacctcctggtcccccaccagcaccacagccgcccagccctgctcctagtccaccacctcggccagccctggccagggaggatgaggaggaagatgaggacgagcccactgaaacagagacatctggggagcggctgggcggtagcgataatggaggtctcttcatgatggatgaggatgccaccctccaggacctgccccccttctgcgagtcagacccggagagcacagacgacggcagcctgagcgaggagacgcccgccggtcccacagcctgtccccagcccccggccacagccctgcctacccagcagtatgccaagtctctgcccgtgtcggtgccagtgtgggccttcaaggagaagaggacagaagcccgatcgtcagatgaggagaatggcccgccctcctcgcccgacctagaccgaatagcggccagcatgcgcgcgctggtgctgcgggaggctgaggacacccaggtcttcggggatcttccgcggccgcggctcaataccagcgacttccagaagctgaagcggaaatattaagcgaacagaaagccttggcctgcgagaggccactgcctctcggcccagcaagagaacaattctttccgggattggctggctcaggttgggcggggccggttctaaacgctccaagggtcaactctgtttcttgcctagcaaacgaagctttgcccgcacagccttgactcgcggccccagggcggagcccagctgcgggagctgccgctgctcagccagtgtcgggaagagttgcccgccagccttccagagttcccctacccgattggtcccggcctcctgggagtgtggccaagccctccctaacccagaattggcccgaggcctttaatttcttacactactagggctgttaatcaattgtttttgtttttgttgttttctttgcctgagtcctgacaattgtatcccggttccccaggggacagtctgggtccctctctggtctgacgctccctataggttccaccgcacgacagcctgtccatcaaaacttgtcctgtacccaggacagtaccagcttcctgtcccccgaccctaacaggtgccttaaaaggccctctcccacccaaggtgggggggccaggggggcctcactttccggccctagacttgggtgcgggagagtgggatgggggtagggaggggcgctctgagattaaagttttacctctgactagtga

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