LOCUS       NM_001033226            1932 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus calreticulin 4 (Calr4), transcript variant 2, mRNA.
ACCESSION   NM_001033226 XM_205476 XM_897727 XM_897738 XM_916228
VERSION     NM_001033226.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Diaz Del Moral S, Barrena S, Hernandez-Torres F, Aranega A,
            Villaescusa JM, Gomez Doblas JJ, Franco D, Jimenez-Navarro M,
            Munoz-Chapuli R and Carmona R.
  TITLE     Deletion of the Wilms' Tumor Suppressor Gene in the Cardiac
            Troponin-T Lineage Reveals Novel Functions of WT1 in Heart
            Development
  JOURNAL   Front Cell Dev Biol 9, 683861 (2021)
   PUBMED   34368133
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Ansarullah, Jain C, Far FF, Homberg S, Wissmiller K, von Hahn FG,
            Raducanu A, Schirge S, Sterr M, Bilekova S, Siehler J, Wiener J,
            Oppenlander L, Morshedi A, Bastidas-Ponce A, Collden G, Irmler M,
            Beckers J, Feuchtinger A, Grzybek M, Ahlbrecht C, Feederle R,
            Plettenburg O, Muller TD, Meier M, Tschop MH, Coskun U and Lickert
            H.
  TITLE     Inceptor counteracts insulin signalling in beta-cells to control
            glycaemia
  JOURNAL   Nature 590 (7845), 326-331 (2021)
   PUBMED   33505018
  REMARK    Erratum:[Nature. 2021 Apr;592(7852):E1. PMID: 33712809]
REFERENCE   3  (bases 1 to 1932)
  AUTHORS   Dey S and Matsunami H.
  TITLE     Calreticulin chaperones regulate functional expression of
            vomeronasal type 2 pheromone receptors
  JOURNAL   Proc Natl Acad Sci U S A 108 (40), 16651-16656 (2011)
   PUBMED   21933956
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK163350.1, AK139540.1 and BG083829.2.
            
            On Oct 24, 2013 this sequence version replaced NM_001033226.3.
            
            Transcript Variant: This variant (2) has alternate 5' exon
            structure and it thus differs in the 5' UTR and 5' coding region,
            compared to variant 1. The encoded isoform (b) has a distinct and
            shorter N-terminus, compared to isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK163350.1, SRR10662773.1061812.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1603              AK163350.1         2-1604
            1604-1912           AK139540.1         1583-1891
            1913-1932           BG083829.2         516-535
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 51.1 cM"
     gene            1..1932
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="calreticulin 4"
                     /db_xref="GeneID:108802"
                     /db_xref="MGI:MGI:2140435"
     exon            1..244
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    91..93
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="upstream in-frame stop codon"
     CDS             94..1356
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="isoform b precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="calreticulin 4 isoform b precursor"
                     /protein_id="NP_001028398.1"
                     /db_xref="CCDS:CCDS18461.1"
                     /db_xref="GeneID:108802"
                     /db_xref="MGI:MGI:2140435"
                     /translation="

MCWFFFFSLSLSPFSTDGWTKRWVQSKHQSDYGQFQLASGKFYQDKERDKGLQTTEDAKFYALSTRFKPFSNENETLVVQFSVKHEQGIDCGGGYVKLFPAALNQEDMHSESQYYIMFGPDICGFGNNRLQVILSHKGKYHENNKTLKCRINKDTHLYTLILRPNATYEVKIDNQKVTSGGLEDDWDFLPPKKIKDPYARKPRKWDERQQIEDPDDKKPEDWEDSEFIPDPDAKKPDDWNEAMDGVWEGPLIPNVKYMGEWKPRIIDNPNYQGEWIHPEIDNPKYRPDPTIGHYHNISVLGLDLWQVKSGSIFDNFLLTNDEEFAEEVGNMTWGLRKDVEQQWRELYEEMEKQKEAEETKKKREKERARQEDVWGLDEEDEEDAEEDDEEEAEERLKQEGSAARASLDQKEAHLDQKDEL"

     sig_peptide     94..147
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    130..1047
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /note="Calreticulin family; Region: Calreticulin;
                     pfam00262"
                     /db_xref="CDD:459737"
     exon            245..448
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            449..543
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            544..753
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            754..867
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1011
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1104
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1932
                     /gene="Calr4"
                     /gene_synonym="4933403L16Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ggtaagcgtgatttcaggaatattctgagtcacctgttactttttccctctgcccgtagaacttaattagcaatggcaacttgattcatttgaatgtgttggtttttttttttttctctctctctctctcctttctccacagatggatggacaaaacggtgggtacaatctaaacatcagtcagattacggccaattccagctagcctcgggaaaattttatcaagataaagagagagataaaggtctccagaccacagaagatgccaagttctacgccctttccaccaggtttaagccattcagcaatgagaatgagaccttggtggttcagttttcagtaaaacacgagcaaggcatcgattgcggcggtggctacgtgaaactctttcctgccgcactgaaccaggaggacatgcactcagaatcccagtattacatcatgttcggcccggacatctgtggctttggcaacaacagactacaggtcatcctttctcacaaagggaaataccatgagaacaacaagaccctcaagtgcaggattaataaagacactcacctgtacactctgatccttcgccctaatgctacttatgaggttaaaattgacaaccagaaggtgacaagtggaggactggaagatgactgggacttcttgcctcccaagaaaataaaagacccctacgccaggaaaccaaggaaatgggatgaacgacaacagatagaggatcctgacgataagaaacctgaggactgggaggactctgaattcatcccagacccggatgccaagaagccagatgactggaatgaggcgatggatggagtgtgggaagggcccttgataccaaacgtcaagtacatgggagaatggaaacctcggatcattgacaaccccaattaccagggcgagtggattcacccagagatagacaaccccaaatacaggcctgaccccaccattggtcactatcacaacattagtgtccttggtctggatctttggcaggtgaaatcaggcagcatctttgacaatttccttctaacaaatgatgaagagtttgctgaagaggttggaaatatgacctggggattaagaaaggatgtggagcagcagtggagagagctgtatgaggaaatggagaaacagaaggaggcggaggagaccaagaagaagagggaaaaggagagagccaggcaagaggacgtctggggactagatgaggaggatgaggaggacgctgaagaggacgacgaggaggaagcagaggaaaggctcaagcaggaaggcagtgcagcgagggcgtctctggaccagaaggaagcccacttggatcagaaggatgaactgtaactgcacagacactgggtgtggccgaagtcagagacggtgcttagagagggaaatgggccctcgcttagcaaattatgggaagtaaaacgactcattctttttagataaactttggatttatagttctactccccttccctgctctatttgctcattggtagaaagggaatgataattttatctcatgaaatggcccttgggactgaatgaggcaatgcctgtaacatgcccaaagtaaatacttgtaaaaaaaatgtagtagttaaaactactatcattatcatggtgtttatgaaattaattgttggtaaataagcttctatataaacttggttcttactttcaactggggtttttcttttgtttaaatatatgcaattcttagtatttatggagcagtgctaacagttgaacccaatgtttccttttttttttttttttttaaatgtattgagcacctaaagccaaaagcagaacatcctttggacgtttggaaaaatacttcatgaaagtcaccttgtttgcctgctgctcttgccttatgtttaaacaattgttacttgtttgcaagtttgc

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