LOCUS       NM_001423712            3948 bp    mRNA    linear   VRT 13-AUG-2023
DEFINITION  Danio rerio myosin light chain kinase 2 (mylk2), mRNA.
ACCESSION   NM_001423712
VERSION     NM_001423712.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Peng X, Feng G, Zhang Y and Sun Y.
  TITLE     PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere
            Assembly and Cardiac Conduction System Construction
  JOURNAL   Int J Mol Sci 22 (21), 11368 (2021)
   PUBMED   34768802
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Chen F, Yuan W, Mo X, Zhuang J, Wang Y, Chen J, Jiang Z, Zhu X,
            Zeng Q, Wan Y, Li F, Shi Y, Cao L, Fan X, Luo S, Ye X, Chen Y, Dai
            G, Gao J, Wang X, Xie H, Zhu P, Li Y and Wu X.
  TITLE     Role of Zebrafish fhl1A in Satellite Cell and Skeletal Muscle
            Development
  JOURNAL   Curr Mol Med 17 (9), 627-636 (2017)
   PUBMED   29521230
REFERENCE   3  (bases 1 to 3948)
  AUTHORS   Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I,
            Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y and
            Bobe J.
  TITLE     Gene evolution and gene expression after whole genome duplication
            in fish: the PhyloFish database
  JOURNAL   BMC Genomics 17, 368 (2016)
   PUBMED   27189481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3948)
  AUTHORS   Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M,
            Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD,
            Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov
            GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W and
            Hertzano R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3948)
  AUTHORS   Tournoij E, Weber GJ, Akkerman JW, de Groot PG, Zon LI, Moll FL and
            Schulte-Merker S.
  TITLE     Mlck1a is expressed in zebrafish thrombocytes and is an essential
            component of thrombus formation
  JOURNAL   J Thromb Haemost 8 (3), 588-595 (2010)
   PUBMED   20002541
REFERENCE   6  (bases 1 to 3948)
  AUTHORS   Zhong H, Zou H, Semenov MV, Moshinsky D, He X, Huang H, Li S, Quan
            J, Yang Z and Lin S.
  TITLE     Characterization and development of novel small-molecules
            inhibiting GSK3 and activating Wnt signaling
  JOURNAL   Mol Biosyst 5 (11), 1356-1360 (2009)
   PUBMED   19823752
  REMARK    Erratum:[Mol Biosyst. 2009 Nov;5(11):1369]
REFERENCE   7  (bases 1 to 3948)
  AUTHORS   Seguchi O, Takashima S, Yamazaki S, Asakura M, Asano Y, Shintani Y,
            Wakeno M, Minamino T, Kondo H, Furukawa H, Nakamaru K, Naito A,
            Takahashi T, Ohtsuka T, Kawakami K, Isomura T, Kitamura S, Tomoike
            H, Mochizuki N and Kitakaze M.
  TITLE     A cardiac myosin light chain kinase regulates sarcomere assembly in
            the vertebrate heart
  JOURNAL   J Clin Invest 117 (10), 2812-2824 (2007)
   PUBMED   17885681
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX927210.9 and CU469363.8.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GFIL01026547.1 [ECO:0000332]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               BX927210.9         168212-168438
            228-501             BX927210.9         169959-170232
            502-587             BX927210.9         170322-170407
            588-660             BX927210.9         173347-173419
            661-1354            BX927210.9         174539-175232
            1355-1554           CU469363.8         2837-3036
            1555-1663           CU469363.8         5236-5344
            1664-1757           CU469363.8         5433-5526
            1758-1867           CU469363.8         8344-8453
            1868-2009           CU469363.8         8525-8666
            2010-2080           CU469363.8         11378-11448
            2081-2209           CU469363.8         11670-11798
            2210-2281           CU469363.8         13956-14027
            2282-2362           CU469363.8         14249-14329
            2363-2495           CU469363.8         14537-14669
            2496-3948           CU469363.8         14755-16207
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="23"
                     /map="23"
     gene            1..3948
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /note="myosin light chain kinase 2"
                     /db_xref="GeneID:558685"
                     /db_xref="ZFIN:ZDB-GENE-080616-2"
     exon            1..227
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    168..170
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /note="upstream in-frame stop codon"
     exon            228..501
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..2576
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /EC_number="2.7.11.18"
                     /note="skMLCK; skeletal muscle MLCK"
                     /codon_start=1
                     /product="myosin light chain kinase 2, skeletal/cardiac
                     muscle"
                     /protein_id="NP_001410641.1"
                     /db_xref="GeneID:558685"
                     /db_xref="ZFIN:ZDB-GENE-080616-2"
                     /translation="

MSLKDSMKCPVPPSNPTTLEAKVDDLRLKVELLLGSSRSCSMCGTCAQHRALQETLTSQLSTQTRLLDSLEKKVMEMRVALEDLTRGLEDRQCERVSVPLVALQRRKSLPVRSSAEEVYICLDRDRKTMPHAFNKPSEKGILLEVNENNGTRREDHQAKKDSTEVKVEPTLPIKRENGTSSVTPRSEQTQKMNKTENKSMPPTQNHVLIPIRTPAIASQKTEAQEIRKVPPPNIKVPVAAAVIIPNTTGQTCTESVNVTPANHILPKTTVTPAAPPLVNNNTPKLTVSEVPKDFKPSHKYPQTSLPPSVAAQRSEQTPVNMPTTKTTTPQDAALFPPTHKPPKTVGLSVTTATKEASSLRQVHSCPNSLQRLQVPGLKADLVHTVTSTLTSKKRATETVGAAKLTSDGIQIKISSATDKNSAQTKSYNPPKLCFKVIDDVSPQPAPFAHRFVALKSSPPPCDTFSIHTREVLGGGRFGKVHKCTETRTGMRLAAKIINTRNSKERDMALNEMQVMNQLSHPNILQLYEAFEVKNQVVLILEYVEGGELFERIVDESSPLTEVDAMVFVKQICEGVQYMHQMYVLHLDLKPENILLVNRSSHQVKIIDFGLARRYKPREKLKVSFGTPEFLAPEVVNFDFVSFPTDMWTLGVVTYMLLSGLSPFLGDDDGQTLNNVLMGNWYFDEDAFEHVSADARDFVSNLLIRERSGRMSATQCLKHPWLNNIFEKAKSSNIVLKSQVLLKKYMARRLWKKNYIAIAAANRFKKIGSSGSLTSLGI"

     misc_feature    1623..2405
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; Region: Protein
                     Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     misc_feature    order(1656..1667,1674..1676,1680..1682,1719..1721,
                     1725..1727,1812..1814,1860..1871,2001..2003,2013..2018,
                     2022..2024,2058..2063)
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:270870"
     exon            502..587
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            588..660
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            661..1354
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1554
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1663
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1757
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1867
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2009
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2080
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2209
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2281
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2362
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2495
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..3948
                     /gene="mylk2"
                     /gene_synonym="mlck2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ccgctctccacccctgggacagttctgcatgcctctattatcagtttactgtttgcgagtccccttctgtgtgctgcctatttctggtgaccagagataaaagaggaatctgacagactgctactgagcttccacaaaacaccagagatcaacgtgtccaaaatccttagcaaacaaaaaacgcacgtttatgctggcattctccgtgtggaccaacatctttacagcacaaccttactttcatgtctctcaaggactctatgaaatgccccgtgcctccatccaacccaacaaccctggaggccaaggtggatgacctgcggctgaaggtggaactccttttgggctcgtccagaagttgctccatgtgtgggacatgtgcacagcaccgtgccctgcaggagacactgaccagtcagctgtccacccagaccagactcctggactctctagagaagaaagtgatggaaatgagagtagctctggaggatctgacacgcggtctggaggacagacagtgcgagagagtgtctgtgcctttagtggctcttcagagacggaaatcactgcctgttcgaagctctgcggaggaagtctacatttgtttggacagagaccgtaaaacgatgccccatgcttttaataagccctcagagaaaggtattttgctagaggtcaatgagaacaatggaacaagaagagaggaccatcaagccaagaaagattcaacagaggtgaaagttgaacctacattgccaattaaaagggaaaacggcacatccagcgtaaccccaagatccgagcaaacacagaaaatgaataagactgaaaataagagcatgccaccaacccagaatcatgtcctgataccgatcagaactccagccattgcttcccaaaaaacagaagcccaagaaatcaggaaagtgccgccaccaaatattaaagttcctgtagcagcggctgtgattattcccaacacaactggacagacgtgcactgaatctgtgaatgtaaccccagcaaatcatatcttacccaagacaaccgtcactcctgcagcgccaccattagtaaacaacaatacccccaaactcactgtatcggaagtccctaaagacttcaaaccatcccataaatatccacaaacaagtctgccaccatcagtagctgcacagaggtctgaacaaacaccagtaaatatgcctacaacaaaaacaacaacaccacaagatgcagcattgtttcctccaacccacaaaccacctaaaacagtgggtttaagtgttaccactgcaacaaaagaggcttcctccctcagacaagtgcacagctgccccaattctttacaaagattacaggtccctggattaaaggcagatttggtccacactgtaacatcaacactgactagtaaaaaaagagcaactgaaactgttggagcagcaaagctaacatcagatggaatacagattaaaataagttcagctacagacaagaattcagcacagacaaagagctacaaccctcctaaactctgttttaaagttatagatgatgtttcacctcagcctgctccttttgcgcaccggtttgtggcgctgaagtctagccctccgccctgtgacaccttcagtatccacaccagagaagtgcttggaggtggccgctttggtaaggtgcataaatgtacggagactagaacggggatgaggctcgctgccaaaatcatcaacacaagaaattccaaagaaagagacatggcactgaatgagatgcaggtcatgaaccagctgagccaccccaacatcctccagctttatgaggccttcgaggtcaagaatcaagtcgtgctcatcctggaatatgtggagggaggcgagctgttcgagaggattgtggatgaaagcagtccgctgacggaggtggatgcgatggtgtttgtgaagcagatctgtgagggcgttcagtacatgcatcagatgtacgtgcttcatctggaccttaagccagagaacatcctccttgttaatcgctctagccatcaggtgaaaattatcgactttggactagccaggagatataaaccacgagagaagctcaaagtttcatttggaacgcctgagtttttagctccagaggttgtgaactttgattttgtctccttccctacggatatgtggactttgggagtcgtcacttacatgcttttaagtggcctctctccttttctgggtgatgatgacggccagaccctcaataatgtgctcatggggaactggtacttcgacgaggacgcttttgagcacgtgtctgctgacgcgcgggactttgtttccaacctactgatcagagaaagaagtggtcgtatgagtgccacacagtgtctcaaacacccatggctcaacaacatctttgagaaagccaaaagcagtaacatcgtgctcaagtcacaggttctcctcaagaaatacatggccaggcgattgtggaagaaaaactacatagctatcgctgcagcaaataggttcaagaagattggaagttcaggttccttgacctcacttggaatctgagtatgttcaaactcttataatacacaggtcatgtctttgtattgctgtgccgcactgcaaatcacctttcacctctgaagtttgactggctgctgattttaattgcattggtaaaccacacaaatctataatgtgcaagaaccatttatttatacaatacatatatatcttgtatattgtttcaagcattcataaattaaacgtatagctataaacactcaccggccactttattgggtacaccttactagtacagggttggacccccttttggcttcagaactgccttaatcctttgtggcatagattcaacagggtaccggaaatattcctgagattttagtccatattgaagtgatagcagcacacagttgctgcagtttagtttgctgtgcatccatgatgcgaatctcacgttccaccacatccgacaggtgctcagttggattgtgatctggtgactgtgaggaccatttgagtacagtgaactcattgtcatgttcaaaaaaacagtctaagatgatttgctctgcatgacatggtgcattatactgctgaaagtagccatcggaagataggtaggcttaggcgttgacacaatgctcaattggtactattgggcctaaagtgtgccaagaaaatatcctccacactattacaccaccaccacgctgaactattaatacaaggcaggatagatccatgcttcatgttgtagacgccaaaatctgaccctgccatcctaatgtcacagcagaagtcaagactcaatcggccaagcaacatttttccaatcttccattgtccaattttggcgagcctttgcaaattgtatcttcagtttcctgactaaatgcattgagttggtgccatgtgattgactgattagaactttgcgttaatgagcagttggacagttgtacctaataaagtggccagtatgggagagcatgtggctaaaatggaaaatgtcctaaataaaagggagcaacttttcttttttttgatctgataatgcctaagattttattattcatattttaaatggccagtaaatgaagaccacgtatccaaaatgtgacaggaaaataatggtggtctgacatattttacgagaaataattgttggttcatgaacaggaagtaaaaacaagaagttcagtatgaatttttgctcgtatcaacaaagtatttcatgcagaagatcctgttttgaaatgtgacgaaacatttatattcatttgctgatgatgatcagactgtaaatacaggtggttaaataagcagtggcatgttcattcaaattgcatgtgtcattaaatttataatgtgacttccagaaataaacgaataagaaagtattggaaaaaca

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