LOCUS       NM_001083856            2472 bp    mRNA    linear   VRT 24-JUL-2022
DEFINITION  Danio rerio taste receptor, type 1, member 2, tandem duplicate 2
            (tas1r2.2), mRNA.
ACCESSION   NM_001083856 XM_001332313
VERSION     NM_001083856.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Del Vecchio G, Murashita K, Verri T, Gomes AS and Ronnestad I.
  TITLE     Leptin receptor-deficient (knockout) zebrafish: Effects on nutrient
            acquisition
  JOURNAL   Gen Comp Endocrinol 310, 113832 (2021)
   PUBMED   34089707
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Gao S, Liu S, Yao J, Zhou T, Li N, Li Q, Dunham R and Liu Z.
  TITLE     Taste receptors and gustatory associated G proteins in channel
            catfish, Ictalurus punctatus
  JOURNAL   Comp Biochem Physiol Part D Genomics Proteomics 21, 1-9 (2017)
   PUBMED   27806254
REFERENCE   3  (bases 1 to 2472)
  AUTHORS   Ohmoto M, Okada S, Nakamura S, Abe K and Matsumoto I.
  TITLE     Mutually exclusive expression of Galphaia and Galpha14 reveals
            diversification of taste receptor cells in zebrafish
  JOURNAL   J Comp Neurol 519 (8), 1616-1629 (2011)
   PUBMED   21452212
REFERENCE   4  (bases 1 to 2472)
  AUTHORS   Oike H, Nagai T, Furuyama A, Okada S, Aihara Y, Ishimaru Y, Marui
            T, Matsumoto I, Misaka T and Abe K.
  TITLE     Characterization of ligands for fish taste receptors
  JOURNAL   J Neurosci 27 (21), 5584-5592 (2007)
   PUBMED   17522303
REFERENCE   5  (bases 1 to 2472)
  AUTHORS   Ishimaru Y, Okada S, Naito H, Nagai T, Yasuoka A, Matsumoto I and
            Abe K.
  TITLE     Two families of candidate taste receptors in fishes
  JOURNAL   Mech Dev 122 (12), 1310-1321 (2005)
   PUBMED   16274966
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB289806.1.
            
            On Apr 5, 2007 this sequence version replaced XM_001332313.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB289806.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA2168448, SAMEA3505384
                                           [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /chromosome="8"
                     /map="8"
     gene            1..2472
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="taste receptor, type 1, member 2, tandem duplicate
                     2"
                     /db_xref="GeneID:664686"
                     /db_xref="ZFIN:ZDB-GENE-070917-2"
     CDS             1..2472
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="taste receptor, type 1, member 2b; zfT1R2b; taste
                     receptor, type 1, member 2.2"
                     /codon_start=1
                     /product="taste receptor type 1 member 2.2 precursor"
                     /protein_id="NP_001077325.1"
                     /db_xref="GeneID:664686"
                     /db_xref="ZFIN:ZDB-GENE-070917-2"
                     /translation="

MLPCYSFLLAFINVVSCLESEFSLKGDYLLGGLFPIHEGKPATHLFTPEAIECFRYTFSKSGYQMLQVMRFAVEEINNSTTLLPDVSLGYEIFDHCSDTKNFPSVLSFISKNGSIKPKLKLNNYEPEVIALTGPYGSTTTITIAPLITMDLIPLVNYGASSYSLSNKLMYPSFVRTVPSNKDLIHMIIQIIQWFGWNWVAFLGDQDDYSEDGLRLFNTFISNSGICLAYQEALSQKTNYSLTFEMIDMLNVNVIVVFAEQQYASNIIKAAIANDVRDKVWIASETWSMNQQLPREPGIEKIGTVIGITERFLSLPGFNEFIYKERRSVDDAGHNHGMGEVKSQTCNQDCDCCTLLTAEEIITENPTFTFGIYSAIYTIAHALHKVLQCDVNVCDKNTTAKPYMLLEQIKKLDFPLNDRQVKYDANGDPTISFAIILWHTETDPPHFDKVGMFDTYPEVTFTINNTLLPWHNNVPFSNCSAECKEGFAREHDRFHTCCFLCKKCPRNSYVDYTRDPYTCFPCAESEWSDEGSTACKNRSVAYLQLTEASSITVLFSATCLMTVLIAIFVLFACNYNTPVVRSAGGSMCFLMLACLIMSTVSVFFFFGEPTFEHCILRNVIFAYFFSVFLSCMAVRSFQIVCIFKMAAKFPSMHSLWVKHNGQWLFVGFFSIINIVSCVLYMTVSPPKPFRDLVTFKDQLILSCEIGNTVTISMVMFIAWFLGFLCLVFSYMGRDLPKNYNEAKSITFSLILYYLSWIVYFTTYLMLKSKYIQLVNAMTELSSIYGILFSYFIPKSYIIIFQPHKNTPAYFQTSIQSYTQTISRT"

     sig_peptide     1..51
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="COORDINATES: ab initio prediction:SignalP:4.0"
     misc_feature    64..1416
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="Type 1 periplasmic binding fold superfamily;
                     Region: Periplasmic_Binding_Protein_type1; cl10011"
                     /db_xref="CDD:471960"
     misc_feature    1420..1581
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="Nine Cysteines Domain of family 3 GPCR; Region:
                     NCD3G; pfam07562"
                     /db_xref="CDD:462210"
     misc_feature    1639..2397
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="type 1 taste receptor subtype 2a and similar
                     proteins, member of the class C of seven-transmembrane G
                     protein-coupled receptors; Region: 7tmC_TAS1R2a-like;
                     cd15287"
                     /db_xref="CDD:320414"
     misc_feature    1639..1716
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 1 [structural motif]; Region: TM helix 1"
                     /db_xref="CDD:320414"
     misc_feature    order(1648..1650,1657..1662,1669..1674,1678..1683,
                     1783..1785,1795..1797,1804..1809,1831..1833,1843..1845)
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="putative dimer interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:320414"
     misc_feature    1750..1815
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 2 [structural motif]; Region: TM helix 2"
                     /db_xref="CDD:320414"
     misc_feature    order(1810..1812,1846..1851,1858..1863,1870..1872,
                     2110..2112,2128..2130,2137..2142,2149..2151,2251..2253,
                     2260..2265,2272..2274,2284..2286,2308..2313,2320..2322,
                     2329..2331,2341..2343)
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="putative allosteric modulator binding site
                     [chemical binding]; other site"
                     /db_xref="CDD:320414"
     misc_feature    1840..1914
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 3 [structural motif]; Region: TM helix 3"
                     /db_xref="CDD:320414"
     misc_feature    1981..2043
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 4 [structural motif]; Region: TM helix 4"
                     /db_xref="CDD:320414"
     misc_feature    2116..2196
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 5 [structural motif]; Region: TM helix 5"
                     /db_xref="CDD:320414"
     misc_feature    2218..2289
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 6 [structural motif]; Region: TM helix 6"
                     /db_xref="CDD:320414"
     misc_feature    2302..2379
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /note="TM helix 7 [structural motif]; Region: TM helix 7"
                     /db_xref="CDD:320414"
     exon            1..164
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
     exon            165..462
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
     exon            463..1209
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1416
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1537
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..2472
                     /gene="tas1r2.2"
                     /gene_synonym="tas1r2b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

atgcttccatgttactcattccttttggcctttattaacgtcgtttcttgcttagaatcagagttcagcctgaagggagattacttattaggtgggctttttcctatacatgaaggtaaacctgcaacacatctgttcaccccagaagccattgaatgtttcagatacactttctcaaaatctggctatcagatgttgcaagtgatgaggtttgcagttgaggagattaacaactccacaactcttcttcccgatgtttctctgggctatgaaatctttgaccactgttctgacacaaagaatttcccttcagttttaagttttatctcaaaaaatggatcgataaaacctaaattaaaactcaacaactacgaacctgaagtgattgctttaacagggccatatggaagcacaaccactataactattgcaccgttgatcacaatggaccttattccactggtgaattatggagcttccagctattcgttgagtaataaactcatgtacccgtcttttgtaagaacggtgcccagcaacaaagacctgatacatatgattattcagatcatacagtggtttggatggaactgggtcgcctttcttggtgaccaggatgattatagtgaagatggcttaaggctgtttaacacgtttataagcaactctggcatttgtctggcctatcaagaggctttgagccaaaagacaaactacagtctaacgtttgaaatgattgatatgcttaacgtcaatgtcatcgttgtttttgctgaacagcagtatgccagcaacattatcaaagcagcgatagcaaacgacgttcgtgacaaagtatggattgcgagtgaaacatggtcaatgaatcagcagctaccaagggagccagggattgagaaaatcggtacagtcattggcattacagagcgattcttgtcgttacctggattcaatgaattcatctataaagaaaggcgatcagttgatgatgctggtcataatcatggtatgggtgaagtcaaaagccagacatgcaatcaagactgcgattgctgcacattgttgactgcagaggagattataactgaaaatccaacattcactttcggtatctattcggccatatataccatagctcacgcactgcataaagtcctgcagtgtgacgtgaatgtatgtgacaaaaacacaacagctaagccttacatgcttcttgaacaaataaagaagctggatttcccactcaatgaccgtcaggtgaaatatgacgcaaacggtgatccaactatcagctttgcgatcatactctggcatactgaaacagatcctccacattttgataaagtgggcatgtttgatacatatcctgaagtaacttttaccatcaacaacactctcttgccctggcataacaatgttcctttctcaaactgttctgctgagtgcaaggaaggatttgcaagggaacacgatagatttcatacctgctgttttctgtgcaaaaaatgtccacggaacagctatgtggattatactcgggacccctatacatgttttccatgtgctgagagtgaatggtctgatgagggcagcacagcatgtaagaatcgttctgttgcctacctccagctcacagaagcctcctccatcactgtcctgttttccgccacatgccttatgacggtcctcattgccatatttgtccttttcgcctgcaattacaacacacctgtggtcaggtctgctggcggtagcatgtgtttcttgatgttagcatgtttgatcatgtctaccgtaagcgtgttcttcttcttcggagaaccaacatttgaacattgcatccttagaaatgtcatctttgcatatttcttctctgtttttctttcctgcatggctgtgcgctcctttcaaattgtttgcattttcaaaatggctgccaagtttcctagcatgcacagcctttgggtcaagcacaatgggcagtggctctttgtcggttttttttcaatcattaatatagtttcttgcgtgctgtatatgactgtctcacctcccaagcccttcagagacttggtaacctttaaagatcagctcattctcagctgtgagattgggaacactgtaactattagcatggttatgtttatagcatggtttctcggtttcctttgtctcgtattttcgtacatgggaagagatctgcctaagaattacaatgaggcaaaatcgataaccttcagtctcatcctgtactacctgagctggattgtgtacttcacaacatacctcatgcttaaaagcaaatacattcagcttgttaatgcgatgacagagttatccagcatttatggaattctcttcagctacttcataccaaagtcttacataataatattccaaccacataagaacactccggcatactttcagacatctattcagagttacacccaaaccattagtagaacttaa

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