LOCUS       NM_001004628            1154 bp    mRNA    linear   VRT 08-APR-2023
DEFINITION  Danio rerio purine nucleoside phosphorylase 5b (pnp5b), mRNA.
ACCESSION   NM_001004628
VERSION     NM_001004628.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Myllymaki H, Astorga Johansson J, Grados Porro E, Elliot A, Moses T
            and Feng Y.
  TITLE     Metabolic Alterations in Preneoplastic Development Revealed by
            Untargeted Metabolomic Analysis
  JOURNAL   Front Cell Dev Biol 9, 684036 (2021)
   PUBMED   34414180
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Kimura T, Takehana Y and Naruse K.
  TITLE     pnp4a Is the Causal Gene of the Medaka Iridophore Mutant
            guanineless
  JOURNAL   G3 (Bethesda) 7 (4), 1357-1363 (2017)
   PUBMED   28258112
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1154)
  AUTHORS   Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I,
            Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y and
            Bobe J.
  TITLE     Gene evolution and gene expression after whole genome duplication
            in fish: the PhyloFish database
  JOURNAL   BMC Genomics 17, 368 (2016)
   PUBMED   27189481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1154)
  AUTHORS   Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM and
            Raible DW.
  TITLE     Interplay between Foxd3 and Mitf regulates cell fate plasticity in
            the zebrafish neural crest
  JOURNAL   Dev Biol 344 (1), 107-118 (2010)
   PUBMED   20460180
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081397.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081397.1, BC165322.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA4476738, SAMEA4476755
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /chromosome="2"
                     /map="2"
     gene            1..1154
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="purine nucleoside phosphorylase 5b"
                     /db_xref="GeneID:447889"
                     /db_xref="ZFIN:ZDB-GENE-040912-54"
     exon            1..73
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    3..5
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="upstream in-frame stop codon"
     CDS             51..935
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /EC_number="2.4.2.1"
                     /codon_start=1
                     /product="purine nucleoside phosphorylase 5b"
                     /protein_id="NP_001004628.1"
                     /db_xref="GeneID:447889"
                     /db_xref="ZFIN:ZDB-GENE-040912-54"
                     /translation="

MTPDSATGYSYEECTATADWLLKRAPERPLVGIVCGSGLGGLAKMLKDELVINYCDIPNFPQSTVHGHAGKLVFGTLKGKPCVCMQGRFHLYEGYAIQKTTMPIRVFKLMGVETVILTNAAGGLNQDFKVGDIMIIKDHINIPGFAGHNPLVGANDDRFGVRFPCMSDAYDRDLQQLVRAVADELDFSVFMRDGVYSVLGGPSFETIAECRALRQLGADAVGMSTVHEVIVARHCDMRVLALSLITNKAVMDYQSEKKANHEEVLETGALRAKQMEKLVSTVVSRIPNTPTHCS"

     misc_feature    84..899
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="purine nucleoside phosphorylases similar to human
                     PNP and Escherichia coli PNP-II (XapA); Region:
                     PNP-EcPNPII_like; cd09009"
                     /db_xref="CDD:350160"
     misc_feature    order(156..161,312..314,318..320,324..326,402..416,
                     645..647,660..665,678..680,711..722,786..797,825..827,
                     831..833,840..842)
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="active site"
                     /db_xref="CDD:350160"
     misc_feature    order(156..161,252..254,312..314,318..320,405..410,
                     720..722)
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="phosphate binding site [ion binding]; other site"
                     /db_xref="CDD:350160"
     misc_feature    order(159..161,318..320,324..326,408..416,660..665,
                     711..719,786..791,831..833,840..842)
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="purine nucleoside binding site [chemical binding];
                     other site"
                     /db_xref="CDD:350160"
     misc_feature    order(321..335,459..473,483..488,492..497,501..506,
                     537..557,564..566,573..575,585..587,633..635,642..677,
                     681..686,690..698,717..719,738..740,798..800,819..827,
                     831..833)
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /note="homotrimer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:350160"
     exon            74..243
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
     exon            244..347
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
     exon            348..523
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
     exon            524..714
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
     exon            715..1145
                     /gene="pnp5b"
                     /gene_synonym="zgc:101740"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

actgagcctcagagcagagtcacggtaagcggacactacagaacgccgacatgactcctgattccgccacagggtacagctatgaggagtgtacggccacagctgattggctgttaaagcgagctccggagcgccctctggtgggcatcgtgtgcggctctggtctgggtggattggccaaaatgctgaaagatgagctggtcataaactactgcgacatcccaaacttcccccagagcaccgtgcacggccacgctggaaagctggtgttcggcacactaaaaggaaagccgtgtgtttgcatgcagggcaggtttcacctctatgaaggatacgctattcagaagaccaccatgcccatccgggtgttcaagctgatgggtgtagagaccgtcattctgaccaacgctgctggaggactcaaccaggactttaaagtgggcgacatcatgatcattaaagatcatatcaatattccaggattcgccggacacaacccgctagtgggagccaatgacgacaggttcggcgtgaggtttccgtgtatgtcggatgcgtatgaccgggatctgcagcagctggtgcgagccgtggcagatgagctggatttctcagtcttcatgcgggatggggtgtacagtgtgctgggcggcccgtccttcgagaccatcgctgagtgcagagctctgcgccagctcggagccgacgccgtgggcatgagcactgttcatgaggtgatcgtggcgcgtcactgtgacatgcgtgttctggctctgtctctgatcaccaacaaggcggtgatggattaccagagcgagaagaaggccaatcatgaggaggttctggagacgggagcgctgagggccaagcagatggagaaactggtgtccaccgtcgtctctcgcatccccaacacacccacacactgctcataacacacacacagactcacttcaggacctgtcagattggctttatgaagtttacagtccacagacggagcatgtagagacccagctcctgttaaaatgtcttaaaatagaagctttaaagggttagttcatgcaaaaatagtgataatcccatgatttgcttacctttaaagggctacgaaacccccccgtctcagtttagtttgaaaaaaaaaaaaaaaa

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