LOCUS       XM_074041448            3820 bp    mRNA    linear   ROD 19-MAY-2025
DEFINITION  PREDICTED: Castor canadensis nuclear factor kappa B subunit 1
            (Nfkb1), transcript variant X4, mRNA.
ACCESSION   XM_074041448
VERSION     XM_074041448.1
DBLINK      BioProject: PRJNA1254772
KEYWORDS    RefSeq.
SOURCE      Castor canadensis (American beaver)
  ORGANISM  Castor canadensis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Castorimorpha; Castoridae; Castor.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133394) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_047511655.1-RS_2025_05
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 05/09/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Castor canadensis"
                     /mol_type="mRNA"
                     /db_xref="taxon:51338"
                     /chromosome="9"
     gene            1..3820
                     /gene="Nfkb1"
                     /note="nuclear factor kappa B subunit 1; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 16 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 32 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:109682963"
     CDS             228..3140
                     /gene="Nfkb1"
                     /codon_start=1
                     /product="nuclear factor NF-kappa-B p105 subunit isoform
                     X4"
                     /protein_id="XP_073897549.1"
                     /db_xref="GeneID:109682963"
                     /translation="

MAEDNPYGGTGQIFHLNALPHTIFKAELFPPDMPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTDACVRGYNPGLLVHPELGYLQAEGGGDRQLTDQEKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGAGGSTGNPGPGYGFPHYGFPTYGGITFHPGTTKSNAGMKHGIINTECENDPEGYDNSDDRETVNLSGKESKPTEQDKESGKSSSGNEVTLTYRVGVKEENACRFQDNLFLEKAMQLAKRHANALFDYAVTGDVKVLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLVSDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRSGADLSLLDRLGNSVLHLAAKEGHDTILSIVLKHRKAALLIDHPNGEGLNAIHIAVMSNSLPCLLLLVASGADVNARERKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTKLAALLKAAGADPLVENFEPLYDLDDPWENAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPEFTPDEQGDMKQLNEDAKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELVEALRQMGYTEAIEVIQAAFCTSGTTVSSPKKNTSQAHLLPHSPSTRQHIDELQDSDSICDSGVETSFRKLSFTESLTGSSSLITLNKMPHNYGQEGPIEGKI"

     misc_feature    345..950
                     /gene="Nfkb1"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD) of nuclear factor of kappa B1 (NF-kappa B1); Region:
                     RHD-n_NFkB1; cd07935"
                     /db_xref="CDD:143651"
     misc_feature    order(387..389,393..398,402..407,414..425,648..650,
                     654..659,948..950)
                     /gene="Nfkb1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143651"
     misc_feature    969..1274
                     /gene="Nfkb1"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; Region:
                     IPT_NFkappaB; cd01177"
                     /db_xref="CDD:238582"
     misc_feature    order(972..974,978..986,990..998,1116..1124,1161..1163,
                     1197..1199,1254..1256,1263..1265,1269..1271)
                     /gene="Nfkb1"
                     /note="ankyrin protein binding site [polypeptide binding];
                     other site"
                     /db_xref="CDD:238582"
     misc_feature    order(978..983,987..989,1026..1028,1032..1034,1038..1040,
                     1137..1142,1149..1151,1155..1157)
                     /gene="Nfkb1"
                     /note="dimerization interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:238582"
     misc_feature    order(1041..1043,1047..1049,1140..1145)
                     /gene="Nfkb1"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     misc_feature    1707..2528
                     /gene="Nfkb1"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     Region: ANKYR; COG0666"
                     /db_xref="CDD:440430"
     misc_feature    order(1845..1847,1851..1853,1863..1868,1875..1883,
                     1887..1892,1902..1904,1911..1913,1956..1958,1962..1964,
                     1968..1970,1980..1985,1992..2000,2004..2009,2019..2021,
                     2028..2030,2055..2057,2061..2063,2067..2069,2079..2084,
                     2091..2099,2103..2108,2118..2120,2127..2129)
                     /gene="Nfkb1"
                     /note="oligomer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:293786"
     misc_feature    1845..1958
                     /gene="Nfkb1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    1962..2057
                     /gene="Nfkb1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    order(2169..2171,2175..2177,2187..2192,2199..2207,
                     2211..2216,2226..2228,2235..2237,2262..2264,2271..2273,
                     2277..2279,2289..2294,2301..2309,2313..2318,2331..2333,
                     2340..2342,2367..2369,2373..2375,2379..2381,2391..2396,
                     2403..2411,2415..2417)
                     /gene="Nfkb1"
                     /note="oligomer interface [polypeptide binding]; other
                     site"
                     /db_xref="CDD:293786"
     misc_feature    2169..2264
                     /gene="Nfkb1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2271..2369
                     /gene="Nfkb1"
                     /note="ANK repeat [structural motif]; Region: ANK repeat"
                     /db_xref="CDD:293786"
     misc_feature    2658..2882
                     /gene="Nfkb1"
                     /note="Death domain of the Nuclear Factor-KappaB1
                     precursor protein p105; Region: Death_NFkB1_p105; cd08797"
                     /db_xref="CDD:260063"
     polyA_site      3820
                     /gene="Nfkb1"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cgcgcgtcctgcgcttccctccgccggcgctgcggacatggcgcggggctgactggcccggcccggcccggtgagaccgcgcgccctatccgcacccgggctcgtccgggcttcggctccggccggccgctacctcttctgtccccaacgccgctggccagcgacgcccggagggggagcccgcaggcgcagggaggccgcgcgcggacccgccaccaggctccagaatggctgaagacaatccatatggagggactggacaaatatttcacttgaatgcattgccccatacaatatttaaagcagaattatttccaccagatatgccactgtcaacagatggcccataccttcaaatattagagcaacctaaacagagaggatttcgtttccgttatgtatgtgaaggcccatcacatggtgggcttcctggtgcatctagtgaaaagaacaagaagtcctatcctcaggtcaaaatctgcaactatgtgggacctgcaaaagttattgttcagttggtcaccaatggaaaaaatatccacttgcatgcccacagcctggtgggaaaacactgtgaggacgggatctgcactgtgactgctggacccaaggacatggtggtcggctttgcaaatttgggaatacttcatgtgacgaagaaaaaagtctttgaaacactggaggcacgaatgacggatgcatgtgtaaggggctataatcctggactgttggtgcaccctgaacttggctatctgcaggcagaaggtggaggggaccgacaactcacagatcaagaaaaggagcttatccggcaagcagcgctgcagcagacaaaggagatggatctcagcgtggtgcgcctcatgttcacggcttttctccctgacagcactgggagcttcacaaggcgtctggaacccgtggtatcagacgccatctatgacagcaaagcccccaatgcatccaacttgaaaattgtaagaatggacaggacagcaggatgtgtaactggaggggaagaaatttatcttctctgtgacaaagttcagaaggatgacatccagattcgattttatgaagaggaggaaaacggtggagtttgggagggatttggagatttttctcccacagatgtccatagacaatttgccattgtcttcaaaactccaaagtataaggatgtcaacattacgaaaccagcttctgtgtttgtccaacttcggaggaaatctgatttggaaactagcgaaccaaaaccttttctctactacccggaaatcaaagataaagaggaagtgcagaggaagcggcagaagctcatgcccaacttttcggatagtttcggtggtggcagtggtgcgggagctggaggcggaggcatgtttggtagtggcggtgcaggagggagtactggaaatccaggtccagggtatggctttccacactatggatttcctacatatggtgggattaccttccatcctggaaccactaaatccaatgctgggatgaagcatggaatcataaacactgaatgtgaaaatgaccctgaaggttatgacaacagtgatgacagagagactgtaaatctctctgggaaagaaagcaaacccacagaacaagataaggagtcgggcaagtccagcagcgggaatgaggttactctgacgtacagggtgggagtaaaggaagagaatgcttgtaggtttcaggataacctcttcctagagaaggccatgcagctcgccaaacgtcacgccaatgccctttttgactatgcagtaacgggagatgtgaaggtgctgctggctgtccagcgccatctcactgcagtgcaggatgagaatggagacagtgtcttacacttagccatcatccaccttcatgctcagcttgtgagagatctgctagaagtcacatctggtttggtttctgatgacattatcaacatgagaaacgatctgtaccagacacccttacacttggcagtgatcactaagcaggaagatgtggtggaggacttgctgaggtctggggccgacctgagccttctggaccgtttgggtaactctgttttgcacctagctgccaaagaaggacatgatacaattctcagtatcgtactcaaacacagaaaggcagcactacttattgaccaccccaatggggaaggtctgaatgccatacacatagccgtgatgagcaatagtctgccatgtctgctgctgctggtggcctctggggcagatgtcaatgctcgggagcggaagtctgggcgcacggcactgcacctggctgtggaacatgacaacatttctttggcgggctgcctactcctggagggtgatgcccatgtggacagtaccacctatgatggaactacacccttacatattgcagctgggagagggtccaccaaactggcagcgcttctcaaagcagcaggagcagatcccttggtagagaactttgagcctctgtatgacctagatgacccatgggagaatgctggagaggatgagggagtggtgcctggaaccacacctctggatatggctgccaactggcaggtgtttgacatattaaatgggaaaccatatgagccagagttcacacctgatgaacaaggagatatgaaacagcttaacgaagatgcaaagctacaactctacaagttgctagaaattcctgatccagacaaaaactgggctactctggcacagaaattaggtctggggatacttaataatgcctttcggctgagtcctgctccttctaaaactcttatggataactatgaggtctctgggggaactatcaaagagctggtggaggccttgcgacagatgggctacactgaggcaattgaagtgatccaggcagccttctgcacctcaggaaccacagtgtccagccccaagaagaacacctctcaggctcacttgctgccccactcaccttccacaaggcagcacatagatgagcttcaagacagtgacagcatctgtgacagtggtgtggagacatccttccgcaaactcagctttacagagtctctgactggcagcagctcattgataactctgaacaaaatgccccacaattacgggcaggaaggaccaatagaaggcaaaatttagcctacagacgatttccctcaccagtaaaccaaaaccctaaaattccagtgcatcgtcccaaagaaggaaggtaaagtgcatccagaggtgctcagaggagaaccagcctgccgggatcattctggatttccttccaggccttctaaacttggcttcctttcttggttcttacatgaattttagttggtccacgtacagatagtatatagcaatcacagcactcggggtgagctgacatgtcgctgggggtgacgggtttattgagctttaccggctgctgctggattacagctgcttcctgttgtccttgctgctgtccctctgctgccgtcccaatgtccccacctggtgatctttctagccgccatggcacaatgttcattcagattaaggattaagaaaagagatattttaaaatgagagtcacttgatatgcaataaaaaggcttattgctttttctaatgtgcttctctgtgatttgaaaaaaaaaaacccatgaacttattaatatttaaacatggctgcaatcagtgccgaaaatagtattttcccctttttctgcattttgctattgtaaatatgttttttagatcaaatactttaaaagaaaaaaaatgttggatataaaatgctattttttattttacttttataataaaaggaaaagcaaattgttgaa

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