LOCUS       XM_072247428            6158 bp    mRNA    linear   VRT 18-MAR-2025
DEFINITION  PREDICTED: Mobula birostris protein argonaute-1 (LOC140190656),
            transcript variant X5, mRNA.
ACCESSION   XM_072247428
VERSION     XM_072247428.1
DBLINK      BioProject: PRJNA1225701
KEYWORDS    RefSeq.
SOURCE      Mobula birostris (giant manta)
  ORGANISM  Mobula birostris
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
            Elasmobranchii; Batoidea; Myliobatiformes; Mobula.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_092399) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_030028105.1-RS_2025_03
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 03/17/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Mobula birostris"
                     /mol_type="mRNA"
                     /isolate="sMobBir1"
                     /db_xref="taxon:1983395"
                     /chromosome="30"
                     /sex="female"
                     /tissue_type="heart muscle"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Marineland, Florida"
                     /lat_lon="29.6689 N 81.2134 W"
                     /collection_date="2010-05-07"
                     /collected_by="Georgia Aquarium"
     gene            1..6158
                     /gene="LOC140190656"
                     /note="protein argonaute-1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 3
                     Proteins"
                     /db_xref="GeneID:140190656"
     CDS             248..2332
                     /gene="LOC140190656"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X4"
                     /protein_id="XP_072103529.1"
                     /db_xref="GeneID:140190656"
                     /translation="

MRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVPGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLVIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVRTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    278..430
                     /gene="LOC140190656"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    431..793
                     /gene="LOC140190656"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(587..589,632..634,674..676,686..688,740..742,
                     761..763,767..769)
                     /gene="LOC140190656"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    926..2203
                     /gene="LOC140190656"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1337..1339,1349..1351,1385..1396,1403..1405,
                     1427..1429,1436..1438,1448..1450,1460..1462)
                     /gene="LOC140190656"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1541..1543,1547..1549,1757..1759,2171..2173)
                     /gene="LOC140190656"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      6158
                     /gene="LOC140190656"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

actacttcgaagttgatattccgaaaatcgatgtttaccattatgaggtggatataaagccagacaagtgtcctcggagagtaaacaggttgattttgaggtgaccattcctggtgaaggaaaagatcggatttttaaggtttccattaaatggctggcagtagtaagttggagactgctgcatgaggcccttgtcagtggtcgaatccaagttccgttagactctgttcaagcattggatgtagcaatgaggcacctggcatcaatgaggtatactcctgttggtcgctcattcttttctccacctgagggttactatcacccactgggaggaggtagagaagtctggtttggtttccatcaatctgtccgacctgccatgtggaaaatgatgttgaacatcgatgtgtccgccacagctttctacaaagcgcagccagtgatagagttcatgtgtgaagtcctagacatacggaacatcgatgagcaaccaaagcctcttacggactctcagagagtgcgttttacaaaagaaattaaaggtttaaaagtggaagtgacccactgtggacagatgaagaggaaatatcgagtgtgcaatgttacacgaaggcccgccagtcatcaaacgttcccactacagctggaaagtggacagactgtagagtgcacagtggctcagtatttcaaacagaaatacaatctgcagctaaaatacccacacttaccctgtctacaggtgggacaggagcagaagcacacttacctaccactagaggtctgcaacatagtaccagggcagcgatgtatcaaaaaactcacagacaatcagacatcaacaatgatcaaagcaacagcacggtcagcgcccgaccggcaggaagagattagtcgattgatgaagaatgccagctacaaccttgatccttatattcaggaatttgggataaaggtgaaagatgacatgacagaagtcacgggccgagttttgccagcgccaattttacaatatggaggccggaatcgagcaattgccacgccgaaccaaggcgtgtgggacatgcgtggcaaacagttctacaatgggattgagatcaaagtctgggccatcgcctgctttgcaccgcagaagcaatgccgggaggaagtgctaaagagtttcactgaccagctgcggaagatatctaaggatgcagggatgccaatccagggacagccatgcttttgtaaatacgcacaaggagctgacagtgtggaaccgatgttcaggcatctaaagaatacctattcaggcctccagcttgtcattgttattctacctgggaaaacacctgtctatgctgaagtgaagcgtgtaggtgatactcttcttggtatggccacacaatgtgttcaggtgaaaaatgttgtgagaacatctcctcagacactttcaaacctctgcctgaagatcaatgtgaaattgggtggaatcaacaacattctagtgcctcatcaacgttcagctgtgttccagcagcctgtgatcttcttgggagctgatgtcacacacccaccagctggagatggcaagaagccctcaataacagcagtggttggcagcatggatgctcatccaagtcgatactgtgctacggtccgtgttcagcgaccccgtcaagagatcatcgaagacctttcttacatggttcgagaactcctcatccagttttacaaatcaacccgtttcaagccaacgaggattattttctatagagatggtgtccctgaaggacaattaccacagattttacactatgagctcctggctattcgtgatgcgtgtatcaaactggaaaaagattatcaacctggaattacctacatagttgtccagaaacgacatcatacgcgactcttctgtgcagataaaagtgagaggattggtaaaagtgggaacataccagcaggaacaacagttgataccaacatcacccacccatttgagttcgatttctacttgtgcagccatgctgggattcagggcaccagcagaccttcgcattattatgtattgtgggatgacaatcgattcacagcagatgagcttcaaatcctgacctaccagttgtgtcacacgtatgtacgctgtactcgctcagtctccattccagcacctgcctactatgccagattggtagcattcagggcaaggtaccacctggtggacaaagaacatgacagtggagaaggcagtcacatctctggacagagtaatgggcgagatccccaggcactggctaaagctgtacaagttcaccaggacaccttacgcacaatgtactttgcttaactctgtgattgtttagtcacttcaacttgccagccttctggactataaacagccatgcaagaacacaaaaccaccttctacagaaaatgtcttagtgttggaaggggtcaacatgtattttacaaaggagcaattttcaaagaactaataactggtggagaaagattgaccaagcagattataggaaagatgtcaaactcttctacatgacaaaaacacatttcacatttgttttattttttacatgtatttatgcaatatattgataacttaatgtcaagtgcaaatgttcaccactgccaaactcctgctagtttttagagacacatggattcaagaaattgcttggtatgcagctacttcatcggaattattcaagaatttttaaaaatcttataggtccaaaagctgaagtttgcaacttagtgcctggattagtaattatttgaatctgtaagtctgatcgacttggcagaatttttttcttacatgttccgaagttggcaccaaacatatcagtcactttcagcctgtttatgttccttgtgatgtctcatttcttttattgtgctttcaaatttcattctaacaagtgcagtaagttgtgtatttacacaatctaatggaatcttaatgttatacaatcactttctaaagtctggactttggaaagttattatgaatatatggaaacagtactaagtcctgaggcagagcaaatggaaacttgtaaattatttttttaaaatatcacattaagaatcttaataagttgtgatggctacacttcactcttactgatattgtgagccacacctgcacagccccctttaaacacaagagggtgtgaatacagcagcaatgaaacgtagatttatagattcagttttagcagacagctgaatgaataaagctgagccttggggtccttttaagaattgctgttctctgaatgtctttagttattcagcagaagaaatgcacactaactgaattcgcacagtagttctcttcagaagctgctaattacttggaagtatatgaggatgaacaagaagcacagtttaagtctgatctttttttatgaagtgaacctgtacaaaggtgaagtttgttttaaatattttaatccttatttggatttgtaatcttcttgctcccacttacagaccacttattttatgaagagagaaggatggtattatgttgacgatgggaaagatttgactgcttgtaatctgaagattttctttaagatttgattttgcaacccactctcaacataaaatatgttccggggcatcaccttattaaggtgtatggaagatatttcctgcattgttggtgtgaatgcttatagtcttaaaagagtgtccctcctctgcaggtatcacacttcatttattggaccaaccatcctgtgaaaccactaaatagaacttttgagcagctttgcctattaatcatactgccaactctaaattgagacaagtacaaacttaaagaagtaattctagctagaaaatcatcctgggacccatgtaatatcccagtgctatatttacagaatttaatgtaaatggaagttcttccacagtactcactccaagattcaatattcatattgtggttcagttttatattcaccatttgcacaagaaaagacaataaggcacaatccagcctaaaaacctatatgtaacactaacattaaaagatcactacaaattgacagataaaatgcttctgtcatgcaggtatgattatcaagagaaaaccatgtaagcatttcacaaaatactttatcctcaccctctccaattttcatccattggaatgtggtgtgttgatgttccctgctggtggatgtctcgtacaaagtatcttttatgtccaatgaaggaaatgaataattaagaaaccattcaaagatcaaggtcatgattgcccactcccttttcccgctcagtatgaccgaagttgctttgcagtagcaaatgttgctttctagcaacagaaatcccgattaatgtgccctttactggataaagtcatttttaacttggccatcttcagcagctgtctgtctgaaattcagtacttgatgcagtcttaaatcacatagaggatgccaaatctcatggctcagtttacatgctgcctttggcagctgctgaattgtagcatcatttttctgaagaaccactgatttttatgttatggcaaagtatctcaagtaacttaaggagcattttcaatttaaatatgtcatcaagccacttagaaagctgttttgggagatgaccataagcttgatcaaaaagttgatttgaggtgtgatttagagcaagaatcttaaagtgcagcatagcgcccaagcatttgaaggcatttatcaaatgcatagcaattttatttggggaagatcaaaagccttgaattgaaggacagcagatatcttgtatggttccagggctggaggagattatagagatcccagcacaaaatatggaagatttaagagcctttttgagaaaaaacactgccccaaatccaactgaaatagatacaagattaaattgacatctataggagaatgatcattacctcaagttactttgtttctgtctcttataactggccatagcagagctttatatttgtgacacctccattaactattaggcatgcaacgcacaaaaagtgggcagatccttataagttggcttatcttggtgtgaagtaacggaagagaagatgcacttgattggaatataactggaattttaaccttccaagaaaagttacagaaaaggggtcatcaaactcctagtaatggaaaagcctgtcgctcatttatggtcagtgtacgatgcactgcctgttggagttcacctgactttagctgaatactgaggaagtggaaaattaaatcaggctgagcaactgttgtctatcccagatatactgtgttttgggatatacagaaacttggaagctagacacctttgcccttaagaaataatgagtaataagaaaagcacaaattaacttcatatatacacaactttcattctccctgctgccttaaataatgaaattcgaacaaggacttagtttacagaggttgattaactgcatattcttggctaatgcaaacacggagggtctttgtggaaatgacacagggctttcccagtaactgaaatatattcagtaaattgctgtgtctcaaattcaatcattgatatttgttgagttagtcgattctaatttgtcacatcattatcttcatattaggcacctgcgtctggtcactctccaccaatccttgctaaagtaacgggtatggattatcaggattggtcatggtgcctaattgctgtaaagatcaacatgaaatcatggaactagaatgaaggtggcgcctttaagttcaaatcagaaaaagagaggagggtaaatctggtttgagcaaagttgaaaggagcttaaaacatccacatttcagcatcatcggttaagaaggtatgagatgatcagtatattatcccccaattatctataagctcgtaactcatcaatccaatcacctggctttacctcctgaagtagagactaatcccgagccatgtctggatattcctgttgaaataaaatgttctcagctaaggtgttgccttggtgctgggagttatgctcgaggcaaaagtagtttattttcttacactgaaccaatatactgaagttaacaatatttataattttctatgtttcttttccttgctgcacaagtgtttttaagaaataaaatgatttgaagcaa

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