LOCUS       XM_058678838            5322 bp    mRNA    linear   MAM 07-AUG-2023
DEFINITION  PREDICTED: Ochotona princeps argonaute RISC catalytic component 3
            (AGO3), transcript variant X3, mRNA.
ACCESSION   XM_058678838
VERSION     XM_058678838.1
DBLINK      BioProject: PRJNA999375
KEYWORDS    RefSeq.
SOURCE      Ochotona princeps (American pika)
  ORGANISM  Ochotona princeps
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Lagomorpha;
            Ochotonidae; Ochotona.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_080833) annotated using gene prediction method: Gnomon,
            supported by mRNA evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_030435755.1-RS_2023_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/28/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Ochotona princeps"
                     /mol_type="mRNA"
                     /isolate="mOchPri1"
                     /db_xref="taxon:9978"
                     /chromosome="2"
                     /sex="male"
                     /tissue_type="muscle"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Grand Mesa, Colorado"
                     /lat_lon="39.087247 N 107.913514 W"
                     /collection_date="2019-07-17"
                     /collected_by="John R. Demboski, Andrea Marie Carrillo"
     gene            1..5322
                     /gene="AGO3"
                     /note="argonaute RISC catalytic component 3; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 mRNA, 3 Proteins"
                     /db_xref="GeneID:101530714"
     CDS             74..1954
                     /gene="AGO3"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X3"
                     /protein_id="XP_058534821.1"
                     /db_xref="GeneID:101530714"
                     /translation="

MCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA"

     misc_feature    74..415
                     /gene="AGO3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(209..211,254..256,296..298,308..310,362..364,
                     383..385,389..391)
                     /gene="AGO3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    548..1825
                     /gene="AGO3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(959..961,971..973,1007..1018,1025..1027,1049..1051,
                     1058..1060,1070..1072,1082..1084)
                     /gene="AGO3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1163..1165,1169..1171,1379..1381,1793..1795)
                     /gene="AGO3"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5322
                     /gene="AGO3"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

aagatgacagtttcagatttcccttggagtttctgccactgccttctacaaagcacaacctgtgattcagttcatgtgtgaagttcttgacattcataatattgatgagcaaccaagacctctgactgattctcatcgggtaaaattcaccaaagagataaaaggtctgaaggttgaagtgactcattgtggaacaatgagacggaaataccgtgtttgtaatgtaacaagaaggcctgccagtcaccaaacctttcctttacagttagaaaatggccaaactgtggaaagaacagtagcgcagtacttcagagagaaatatactctgcagctgaaatacccacaccttccctgtctgcaagtggggcaggaacagaagcatacctacctgcccctggaagtctgtaatattgtggcagggcaacgatgtatcaaaaagctaacagacaatcagacttccactatgattaaggcaacagcaagatctgcaccagatagacaggaggaaattagccgattggtaagaagtgcaaattatgagacagacccgtttgttcaggagtttcagtttaaagttcgggatgagatggcacatgtcactggccgcgtactcccagcgcctatgctccagtatggaggacggaatcgaacagtagcaacaccaagccatggagtatgggacatgcgaggaaagcagttccacacaggagttgaaatcaaaatgtgggccattgcttgttttgccacacagaggcagtgcagagaagaaatactgaaggggttcacagaccaactgcggaagatttccaaggatgcagggatgcctatccagggccagccctgcttctgcaagtacgcacagggggcagacagcgtggagcccatgttccggcatctcaagaacacctactcagggctgcagctcatcatcgtcatcctgccaggaaagacaccagtgtatgctgaggtgaaacgtgtgggagacacacttttgggaatggctacacaatgtgttcaagtcaagaatgtaattaaaacatctcctcagactctctcaaatttgtgcctgaagataaatgttaaacttggagggatcaataatattcttgtacctcatcaaagaccatctgtgttccagcaaccagtgatctttttgggagcagatgtcactcatccacctgctggtgatggaaagaagccttctattgctgccgttgttggaagtatggatgcccacccaagtagatactgtgccacagttagagtacagaggccccggcaggagattattcaggacttagcctccatggttcgggaacttctcattcaattttataagtcgacacggttcaaacctactcgtatcatcttttatcgggatggtgtttcagagggacaatttagacaggttttgtattatgagctgttagcaattcgagaagcttgcatcagtttggagaaagactatcaacctggaataacctatattgtagttcagaagagacatcacactcggttattttgtgctgataggacggaaagggttggaagaagtggcaatatcccagcgggaacaacagttgatacagacattacacacccatatgagtttgatttttacctctgtagccatgctggaatacagggtaccagtcgtccttcccactatcatgttttgtgggatgataactgctttactgcagatgaacttcagctgctgacttaccagctctgccacacatatgtacgctgtacacggtctgtttctattcctgcaccagcgtattacgctcacttggtggcattcagagccagatatcatctcgtggacaaagaacatgacagtgccgaaggaagtcatgtttcaggacaaagcaatgggcgagatccacaagctctcgccaaggctgtacagattcaccaagataccttacgcacaatgtactttgcttaaagagtccaagtatattctctgagaggaagaactgcattgacacacagcctatgttgagtgaagtcaatcgagcaaggagggacgcctccagccatacagaaaccaacacagtggggcccagggtcagatccttatgttggtacaatgaagattgtctgcttcatcaaggaaggcagcaccattatgcaatatgaaaccagccaactgctttttgtgcagtctccgtaggaagtattgcaattgttttcttttcacttcttatagtctaaccgttttaatgcctttacctcaagttgcttggcagcacagctatctttgcaaaaaaaaaaaaaagaagaaaatgtaaatgatggttgaaaaaatacatacctacaggaataatcactgattgttcacaattatgtgcaaaaaaattaacatgcactcatgtattatcttgtaaaagtagtctgtgtatttttaaaatatatacatgcatacttcctatgcatatgtatctggatctagactgataacagatatatatgtatctgtgtatatattttagagctcattccattggggaagcgtctccctttattcccccgtctttgtttccctctcacactgcttgccttcatcagctgtattttttctcctctgatgctgccagtgacattcaaatgttcatgcatctaattcacttgactgtgaagcatggcaaactagatttctatatttattttgcacaccctactcctgtctcctgtctcatacacaattttgagtcctcaaccctgatagtgctttaagaaacaaaattattttctatctgtatctttacacagtgttgtcatatgtctccagactgaacttcacatttgcccctgagcaatgtgtgcataagcagtagcaacctagtattctttatcttcagtgagtttatcaaagctgctacaaatatgaaaccttgtcagaaattgtaactcttcctctctgataagtgacatgtattctaaagccttctgctcctacaggacagacatgaaaattccttaaagcttttataaaggataaatattttaaaaggttggaactctgtagggttgaggcctctgaaattgagtagaggaaagaggattatggagtttttgttttgttttgatttgctttttgctgggttacttgctgcctctcaaaccttaatgtgattttcatatatatttttatatatatgtatgtttgtatatatatatatatataaacatatatgtatgtgttttgaattatagcttccttttcttttatatttattagaatagtgctttaataattataggaccgtgttgacagcatcttttatagaaatataatcatgtttattttaagaactgacaacttgttttttgctatcttttagtgcaataagcagttttaaaggaaagttgtatctttttgacatctctaccatgggtaatgggaggaatgggcacctccataattttataaggatgaagtaatacagccttaatttcagaagggagattttataattttcagttttaaagcatacctttaaaaatcgtaatgaaaatgaagaaagcctaattgcttgcctgagtttgaccttagatcttaaaaataaagttgaacgttaacaagtgacaataaacaacccaacacttaagagatgtctaaattatttgaaaatagattatgtttattgaagattcataccagccaggtgccaatttttatttactaagatgaatcaagaaagtgtgaattgatattttaaagagaatttttgctgatttatcaccagtatgactttccttttaaaactaatgtgatactggcaaaggaagtcgagacttcgatcattttaaattatttaaatcactaaaatgtgggtggggtggggggagttctgcaaaactttcgtctgttgatttcactttaagatgttaagtactaaaaagctgtcaaaaccaagtgtatcaataacattggattcagaggagttttatgccttatagttcatgaaaattgcgttttgactttttttgtttgcactggttaagagtatagatatttcagattgcttccagcgggcattgttattagcctttcagaattccatctgcacatgaaaactaatggatttagatctttagtttgtctcttaccttcaatccactaacattgccaatttactgtattttgagcctaaaatctgagcccatgcccttcaataggcatgaaatcttttgtcccaatgccagaaaactaagaaagtactgtattttgtatctttattattttgaagcatctgaaatgagatttacgatactgtttcaaagcactttacataatatttatatagtatatatacatttttttcctcccagcctgctaccatctcctcagaacaaaaaaaaaaaaatcccattaaagtatgcttgttttcttacattgaaacttcacggaatagagtcaggagcatgagctagagaagacagccatataccctgggtggtgcaagttttatttttctgtgcttgctcagtatgaataaaatgtcttccttcattcgtgggagctacagagactcatgtggaaaatttcagggagcaactgtagctaccctttccctactcactgcttaccctaatctccagtcacttagtgtcacacgtgctagggagaatccgtgaaattaaatgtacttggaaggacattttttaagatgaaaagtattttttctaaaatatagagctttaccttttgagtgatttcttttttagagacttaacactattcagcattgttagtttaaggttgattaacttgaaagaatatgtatagtatatttctattttgcttactaacaaattgatatataaacctaattatttaaaaaaccttatgcatagagttttaagataaaggctaagtggcaaatattggaatttgttcttattattattgcacttttggtgcagaattcctaatctttaaatttttaaaaaggttgctttgagtttctttgtaaatttaaatatgaaactcagaatgatctctctgcttctacccttgctcatgactgaatgctaaattttgatctgctattttctgtgatatgtcatataagagagactttaaaatgtcacccccagcaactgttaaaaatgattttaatgtgaagaagggttgggagtttaatgtatattcctgttggcaatgtctgtgaaaagtgcagatacatgttcttatgttacagtattgagtaactggcattgaggactacgtgtttataatgctgtagctgctatatttatttaagtagggtctgacagttactgcactaaaccttaagatgaacagga

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