LOCUS       XM_058377107            5748 bp    mRNA    linear   VRT 23-JUL-2023
DEFINITION  PREDICTED: Hemibagrus wyckioides argonaute RISC component 1 (ago1),
            mRNA.
ACCESSION   XM_058377107
VERSION     XM_058377107.1
DBLINK      BioProject: PRJNA996633
KEYWORDS    RefSeq.
SOURCE      Hemibagrus wyckioides
  ORGANISM  Hemibagrus wyckioides
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
            Bagridae; Hemibagrus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_080732) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_019097595.1-RS_2023_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/20/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Hemibagrus wyckioides"
                     /mol_type="mRNA"
                     /isolate="EC202008001"
                     /db_xref="taxon:337641"
                     /sex="female"
                     /tissue_type="blood"
                     /dev_stage="sexual maturity"
                     /linkage_group="LG23"
     gene            1..5748
                     /gene="ago1"
                     /note="argonaute RISC component 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 44
                     Proteins"
                     /db_xref="GeneID:131344658"
     CDS             435..3011
                     /gene="ago1"
                     /codon_start=1
                     /product="protein argonaute-1"
                     /protein_id="XP_058233090.1"
                     /db_xref="GeneID:131344658"
                     /translation="

MEPGPSGAVPMGLYPPPLQQVFQAPRRPGMGTVGKPIKLLANYFEVEIPKMDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLDSVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVRDDMAEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    537..929
                     /gene="ago1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    957..1109
                     /gene="ago1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1110..1472
                     /gene="ago1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1266..1268,1311..1313,1353..1355,1365..1367,
                     1419..1421,1440..1442,1446..1448)
                     /gene="ago1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1605..2882
                     /gene="ago1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2016..2018,2028..2030,2064..2075,2082..2084,
                     2106..2108,2115..2117,2127..2129,2139..2141)
                     /gene="ago1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2220..2222,2226..2228,2436..2438,2850..2852)
                     /gene="ago1"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ggaggaggagaaggaggaggaggaggatgaggagggtgtttcagggggagaccgattgcctgcctgactgtttgaccgttttttgttgttgttcctctggagagtcgtgtttggacggtgagcaggaacataaacaggaagaaaaaaagaagaagaaggaggaaaaaaagccctagcgatatctccagcggaaacaagaccgcagtctggaggagagggagaaggagaaagcagccgaaggggagctattgtgttcggggtccgtggagggctccgactcgggggttcagccgggagagggggggggataaagagccgctaggacgccgaaggaaggagcctgagagtcggacacgcttccaggctcgccgccgtggcgatttaactgttccggagaataacgttaaagccgcgggggagcctgagtgaatgggatggagccgggaccatctggagctgtgcccatggggctgtaccccccgcccctgcagcaggtcttccaggctccccggaggccgggcatgggcaccgttgggaagcccatcaagctgctggctaactactttgaagtggagatccccaagatggacgtctaccactacgaggtggacatcaaacccgacaagtgtcctcgtcgagtcaacagggaagtcgtggagtacatggtacagcatttcaaacctcagctcttcggcgaccgcaagccggtgtatgatggcaagaagaacatctacaccgtcctggctcttcctataggaagcgaaaaggtggacttcgaggtcaccataccaggtgaggggaaggaccggatctttaaggtttcgatccgctggctggcgaaggtgtcatggaggctgctgcaggagacgctggtgagcgggcggctccaggtcccgctggattcagttcaggctctggacgtggccatgagacacctggcatccatgaggtacactccagtgggccgctccttcttctctccccctgaagggtactaccatcccctgggaggaggtagagaggtctggttcggcttccatcagtccgtacgcccggctatgtggaagatgatgcttaacattgacgtgtccgccacggctttctacaaagcccagcctgtcatcgagttcatgtgtgaggttctggacatccgcaacatcgacgagcagcccaagaccttaaccgattctcaaagggttcgcttcaccaaggaaatcaaggggctgaaggtggaggtgacgcactgcggtcagatgaaaaggaagtatcgtgtgtgtaacgtcactcgccgtcctgccagccaccagacgttccctctgcagctggagagcggacagacggtggagtgcacggtcgcgcagtattttaaacagaagtacaacctgcagctgaagtaccctcacctgccgtgtctgcaggtcggccaggagcagaaacacacctacctgcccctggaggtgtgtaacatcgtagcagggcagagatgcatcaagaaactgaccgataatcagacgtccactatgatcaaagccacagcacgctcagcaccggacaggcaggaggagatcagcagactgatgaagaatgctaattttaatctggacccctacattcaagagtttgggatcaaggtgcgggacgacatggccgaggtgacgggtcgagtcctgccggctcccatcctgcagtacggaggcaggaaccgagctatcgctacacccaaccagggagtgtgggacatgcgggggaaacagttctacaacgggatcgagattaaagtgtgggccatcgcctgcttcgcccctcagaaacagtgtcgtgaagaggtgctgaagaacttcacagatcagctacgcaagatctcgaaagatgccgggatgcccattcagggtcagccgtgtttctgtaaatacgcccagggagccgacagcgtggagcccatgttcagacaccttaagaacacctactccggtctgcagctcatcatcgtcatcctgcccgggaagacacctgtctatgcggaggttaagcgtgtaggagacactctcctaggaatggccactcagtgtgttcaggtgaagaatgttgtgaagacgtcaccgcagaccctctccaacctctgcctgaagatcaacgtcaaactcggcggcataaacaacatcctggtaccacatcaaaggtccgcagtgttccagcagcccgtcatcttcctgggtgcagacgtcacacacccaccagccggagacgggaaaaagccctccatcactgcagtagtaggcagtatggacgcccaccccagtcggtattgtgccacagtgcgtgtacagaggccgaggcaggagatcatcgaagatctctcctacatggtgcgtgagctcctcatccagttctacaagtccacccgcttcaaacccacccgcatcatcttctacagggacggcgttcccgaagggcagctaccacagatcctgcactatgagcttctggccatccgggacgcctgcatcaagctggagaaggattaccaacccggcatcacctacatcgtggtgcagaagcgccaccacacccggctgttctgcgcagacaagtcagagaggattgggaaaagtggcaacattccagcaggaactacagtcgacaccagcatcacacaccccttcgagtttgatttctacctgtgcagtcatgcaggaattcagggcaccagtcgcccgtcacattactacgtgctgtgggacgacaatcgcttcaccgccgacgagctccagattctcacctaccagttgtgccatacgtacgtgcgctgcacacgttctgtctccatccccgcacccgcctactacgcccggctcgtagccttccgcgcccgctaccacctggtggacaaagagcacgacagtggcgaaggaagccacgtctcaggtcagagtaacggccgagacccccaggctctggccaaagcagtgcagattcaccacgacaccctgaggaccatgtacttcgcctgagagccctgacctggcgccgacatcgaagagcctcgtccacctccctgcgctcgagccagtacacactacaaatccctctcacctcaacaaacagaactaacacagcgtcgtgccctaacctggcaggactgagcctcgtttacttttcgttcttgttgttgattgattgattattttttgtttgtttttaaaaagtgtatcgttgtttactaccttcacaccgtatgtatgtatgttcgtatgcaatacggaggagaagcaaaggcacaaacccacccgaagagctgactgccaatacggcgcttcgtcatttctcgcatcagtgttcaacggaggaaaagaaaaagcgagcgtcgaacaccttggtgttgttttaatggacaattttttttttttttaattgtgtttttattttttgaacagccttgaatgaaatattttagcacttagagttagcttttagacacaggcgtctccccttagctctcgtctctgtacttcggaccgtagcgcgtcggcggatgctggagagacgcagcgctctcgagtgtagccgatcttatttagacgtgcctccgtagtcaggggcggcactagcataacgatagatagtgtttcttttttatggaagtccaaaggggtcatacttttttttattattatttactttttctcgatcacacaggcagctttaagctccactgcatgccgcatagataaggctaggtataaggctgaagtggtagctatcgagttaaaggtagtagttcaacccgaatcaaatgtcgattgttatttcctcccaaatctagtcaaatgatccgtaatacgtgtgacgacgactttagatttgccctgatgctctgaggctaatgtagctaaaaaaaaataaataaatgaaagaacgcttatagcctccgctttagcatcgtgcaaaacgcagatctggataacaaaagaatcagactttggtaggagattttaaaggtggagttatttttgaggtaaaaggggaaatccggtacattttattcttcggatcaaaacatcagggtacacacccttgttcacaccctgtgtttcgcattggagttaaatccagggcgtattcctgcctcacacccagtattcccaggatagactccacctaaccaggataaaccattctgatgaatgactgattaaatgtttcactaatatacaagatagctagctcgttttatttctaagattttgtccatttcccaattggaaatctcggtattcccagtcagggaaccccaaaaacatggcctctatggacttccatacatttaagctaagttccttctacgtgcgcaacacagaaaaccagcattctgaaccagccaaatgtgatgccgaccattcagagaggcacttatctgtttttttgaaatgtgcatctgtttagagtctgtatgtgaggaaattgaaagtgcagcattctggcacgctgtagataaataagagagagagagagacgcaagatcacacacacacacgcaaatctttccttcctattccacaagagccttccagcatctctaaactggtctgtactcaaagcaatacaaggtcttttcacagtggacgtgaacgctctctgctcacgctgcacattccacacaccaagaaggacgtgacacacttcccctcccgccttctctcattcttacgctaactcaaaggacacaggaacatcgggtttttttgttttttttttagtattgttgtgagtacacaccgtgtagctgcccaccaccacctcctacgtagaaagctctgccgtcgggtcgggtcacgcacaacccacccatcctacacacaccctacacacacccacacacccacccacaccacagccttatgattaagaagtcacatccttccacgtagcagccatggtacatacacacctacatatctgcctggatttctgccagatgtgtaccatgtacacagaaatagttgaaaacgggaatttttattattgtttttggttattttttttacccatccatgtcatcgtggtttcatgtattgcatgttttaaatcgtgacccggatccctcagtatccatgctctcttatgttttctagttgttttattaatcgttaactgcacttgttgccccttcggttccttttccgaggggggtcactgtaaggggaggtgggtgggggtggtgtcttcgctttcactttcacttattttttagcctcaaaaagcacctcaaaacaaacatagggtccatcggaattccaaagggatcttggttatgtaatggttgaataggtttacttttcgtttttcttttattaaaaaaaaaatgagtattatgtttcagccaactcgaaatgtatttataacttgaaatggccgctgacggttgggcctatagaattgtgagggctctgatctgatctggtctggtccgatccagcagttttatatcatgatgcagtacttaaactcacaaatcaaatgagaggggagagaaaagaaagaagaaaaaaaaaaaacaagcccctttagacccaaagagaaggaactggagatggaacttgactcagcctcactataaaagcaatacaaagccactgcaaaacgacggcccttatcttagcacttcccaacaggctgattcggaagtacacatgaacaaagttattttggctctgtaatgtaatagtgttggggggagggttatttttt

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