LOCUS       XM_046154475            3229 bp    mRNA    linear   PLN 28-JAN-2022
DEFINITION  Microdochium trichocladiopsis putative RNA interference and gene
            silencing protein (B0I36DRAFT_32743), mRNA.
ACCESSION   XM_046154475
VERSION     XM_046154475.1
DBLINK      BioProject: PRJNA801207
            BioSample: SAMN06297163
KEYWORDS    RefSeq.
SOURCE      Microdochium trichocladiopsis
  ORGANISM  Microdochium trichocladiopsis
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Xylariomycetidae; Xylariales; Microdochiaceae;
            Microdochium.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Mesny,F., Miyauchi,S., Thiergart,T., Pickel,B., Atanasova,L.,
            Karlsson,M., Huettel,B., Barry,K.W., Haridas,S., Chen,C., Bauer,D.,
            Andreopoulos,W., Pangilinan,J., LaButti,K., Riley,R., Lipzen,A.,
            Clum,A., Drula,E., Henrissat,B., Kohler,A., Grigoriev,I.V.,
            Martin,F.M. and Hacquard,S.
  TITLE     Genetic determinants of endophytism in the Arabidopsis root
            mycobiome
  JOURNAL   Nat Commun (2021) In press
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 3229)
  AUTHORS   Haridas,S., Mesny,F., Miyauchi,S., Thiergart,T., Pickel,B.,
            Atanasova,L., Karlsson,M., Huttel,B., Barry,K.W., Chen,C.,
            Bauer,D., Andreopoulos,W., Pangilinan,J., LaButti,K., Riley,R.,
            Lipzen,A., Henrissat,B., Kohler,A., Grigoriev,I.V., Martin,F.M. and
            Hacquard,S.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2021) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NW_025763553).
            
            ##Metadata-START##
            Organism Display Name :: Microdochium trichocladiopsis
                                     MPI-CAGE-CH-0230
            ##Metadata-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Microdochium trichocladiopsis"
                     /mol_type="mRNA"
                     /strain="MPI-CAGE-CH-0230"
                     /db_xref="taxon:1682393"
                     /chromosome="Unknown"
     gene            1..3229
                     /locus_tag="B0I36DRAFT_32743"
                     /db_xref="GeneID:70184021"
     CDS             97..3195
                     /locus_tag="B0I36DRAFT_32743"
                     /codon_start=1
                     /product="putative RNA interference and gene silencing
                     protein"
                     /protein_id="XP_046007728.1"
                     /db_xref="GeneID:70184021"
                     /db_xref="InterPro:IPR003100"
                     /db_xref="InterPro:IPR003165"
                     /db_xref="InterPro:IPR014811"
                     /db_xref="JGIDB:Mictri1_32743"
                     /translation="

MSAPGRGGSFRGGGGGRGPRRGGFGGDSSRARSARGGFQGRGRGGVTVSVFSTPDRIIPVPNTIVAKTEDALLAATKGGLSLAGSKLREGFPCRPGFGTKGTPVTLWANYFEMCPPPKVMFYRYDIAVSPAVAGRKLSQVVRLLLESEELMEFRLDVATDFKSTLVSRQKLPADETVAKIQYRSEDEDEPRQNATQYEVRLLYTNTLSVSHLIEYLTSTNISARFNDAQSLVQAFNIFLNHHSKLTGNLATIGASKKFSLADNADKWDLGTGLTAIRGFFSSVRVATSRLLVNVNVSHGAFYEAGPLDKIMFMYYSAHQRSKYKLNNFLAKLKVGPIHLPERKNKKGEIVRKVKTIVGLANTNDGHGLQHPPRVKEFGAGPKDVEFWLEGGGGGQPASSPGAGSQEEGKKKKQKWKQAGPSDKPSSTSSSGGYISVYEFFWTSESLNACWGAHFIITVRIEYNWQTNPMIPIINVGTRDTPSYLPPEVCVVVPGQNSNSKLNPAQTQQMIQFAVRSPWENAASIVRDGFQTVGLSSQANVLLDRFGVSVPPQLITVSGRVLNEPKVLYKEGKPAPVRSGSWNMVNVKLNTTGTMTNWSYLLISISDRRDAFDQAGLIAMLVKFQEALRKTGMVVAPPALGRRLVLRDQDDPELDNVFKLAAKSLHLLFIILPDTNPLYNRIKHCGDVKYGIQTICVVGHKIAKEKGQDQYFANVALKLNLKLGGNNQLLDKPRLGIINENKTMVVGIDVTHPSPGSSSRAPSIAGMVASVDRWLGQWPAVLRVQAGARQEMVTDLSDMLKSRLQLWKTKGHHQSLPENILVYRDGVSEGQYDKVIDEELPRLRKACTEVYPTSDQKKDLPHFTIVIVGKRHHTRFYPTKEANADRSGNPKPGTIVDRGVTEARNWDFFLQAHAAIQGTARPGHYVVVLDEIFRARYAKTLPPDCSNVADVLEDLTQSLCYVYGRTTKAVSLCTPAYYADIVCERARCYLSGVFDTPTQSAEPSLAGSQTEGLQVGNEDVRIHEKLKDTMFYI"

     misc_feature    376..3189
                     /locus_tag="B0I36DRAFT_32743"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1720..3063
                     /locus_tag="B0I36DRAFT_32743"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2128..2130,2140..2142,2176..2187,2194..2196,
                     2224..2226,2233..2235,2245..2247,2257..2259)
                     /locus_tag="B0I36DRAFT_32743"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2338..2340,2344..2346,2566..2568,3031..3033)
                     /locus_tag="B0I36DRAFT_32743"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ccggctagctcctttttctcctgctctggtttcagttttgatatctgacgttccagagccgagatatcttctcggtcctactcacctattcctgttatgtccgctccaggtcgtggtggctcgtttcgtggtggcggcggaggacgtggccctagacggggaggttttgggggtgattctagtagagcccgcagtgcccgtggaggcttccagggcagaggaaggggcggcgtgactgtttcggtgttctcgactcccgatcgaatcatccctgttccaaacacgattgtcgcgaagaccgaagatgcccttctggccgctaccaaaggtggccttagtctcgcaggctcaaagttacgagaggggtttccttgtcgtcccggcttcggcacgaaaggcacacccgtgacgctgtgggccaactatttcgaaatgtgtccacccccaaaagtcatgttctaccgatacgatattgctgtttcacccgcggtcgccggaagaaagcttagccaggtcgtcaggctgttactggagtccgaagaattgatggaatttcgactggatgtggccactgacttcaagtcaactcttgtgtcgcgtcagaagttgcccgcggatgagacggttgccaaaatccagtaccgctcggaagatgaggacgaacctcgacaaaatgccacccaatacgaagttcgtctcctatacacgaataccttgagtgtctcgcatctgatcgagtatctcacatctacgaacataagcgcgcggttcaacgacgctcagtctctggttcaggccttcaacatcttcctcaaccatcactcaaagctgacggggaatttggctacgattggggctagcaagaaattctctctagccgataacgcggataagtgggatcttggcacaggattgacggcaatacgaggcttcttttcgagcgtgagggtggcaacctcccgcctcctcgtcaatgtgaatgtcagccacggtgccttttatgaagcagggccgctggacaagatcatgttcatgtactactctgctcaccaaagaagcaagtacaaactgaacaacttcctcgcgaagctgaaggtggggcctattcacctgccagaaaggaagaacaaaaagggtgaaatcgtgcggaaagtcaagaccattgttggcctcgccaacacgaatgacggacacggcctccagcaccccccaagggtaaaagagttcggtgcaggtcctaaggatgtggagttctggctcgaaggcggtgggggaggccagccggccagctcaccaggagctggaagtcaggaggaaggtaaaaagaaaaagcagaaatggaaacaggcaggcccttccgacaagccatcgtcgacctcgtccagtggcggatacatcagtgtttacgaattcttttggaccagtgagtctctgaatgcctgctggggagctcattttataataactgtccgaatagaatataattggcagaccaaccccatgatccccatcatcaacgtcggtacacgagataccccaagctatcttccacctgaggtctgtgtcgtcgttccaggtcagaactcaaactcaaagctcaaccccgcgcaaacccaacaaatgatacagtttgctgtacggagcccatgggagaatgctgcttcaatagtccgcgatggttttcaaacagttggtctttcatcacaggcgaacgtgcttctggatcggtttggcgtatcggtcccacctcagttgatcactgtgtcagggagggttctcaacgagcctaaggtcttgtacaaagaggggaagcctgccccggtgcgctctggcagctggaatatggttaacgtgaagttaaacaccaccgggactatgacgaattggtcgtacctactcatttcgatatctgatcggcgggacgcatttgatcaagcaggtctcatcgctatgctcgtcaagtttcaagaggctttgcgcaaaaccggaatggtcgtggcccccccggctctgggacgaagactagtcctgagagaccaggatgacccggagctcgacaacgtcttcaaattagctgccaagtccttacacctgcttttcattatcctaccagataccaaccccctctacaacaggatcaaacactgcggcgacgttaagtacgggatccaaactatctgcgttgttggccataagattgccaaagagaagggccaggaccagtacttcgccaatgtcgccctgaagctcaacctgaaactgggcggaaacaaccagctactcgataaaccacgccttggtataatcaacgagaataagactatggttgtcggtattgacgtcacacacccgtctcctgggtctagttctcgcgcgccaagcatcgccgggatggttgccagcgttgataggtggcttggccagtggcctgcagttctccgggtccaggccggcgctcgccaggaaatggtcacggacctgtcagacatgcttaaatcacgcctccagctgtggaaaaccaaaggacaccaccagtccctgcccgagaatatcttggtataccgtgacggcgtctccgagggtcagtatgacaaagtcatcgatgaggaacttccgcggctgcgcaaggcatgtacggaggtgtatccgacctctgaccagaaaaaggacctcccacatttcaccatcgtcattgtcggtaagcgtcaccacacgcgattttacccgaccaaggaagccaacgccgaccggtccggcaacccgaagcccggcaccatcgtcgaccgcggtgtcacagaggcacgtaactgggacttcttcctccaagcgcacgcagccatccagggcaccgcgcgtccagggcactacgttgtcgtgctcgatgagatcttccgagctcgctacgccaagacgttgccgccggactgcagcaacgtggcggatgtgcttgaggacctaacgcagagcttgtgctatgtgtatgggcgtacgaccaaggcagtcagtctgtgtacgccggcttactatgctgatattgtctgtgagagggctaggtgctatctgagtggtgtgtttgacacgccgacacagtccgcggagccgtcactggccggaagtcagacggaggggttgcaagttgggaatgaagatgtgaggattcatgagaagttgaaggataccatgttttatatttaagcagcggaagaagcggggaagctgggatcttgca

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