LOCUS       XM_030411518            6795 bp    mRNA    linear   VRT 22-JUL-2025
DEFINITION  PREDICTED: Sparus aurata argonaute RISC component 4 (ago4),
            transcript variant X2, mRNA.
ACCESSION   XM_030411518
VERSION     XM_030411518.1
DBLINK      BioProject: PRJNA558036
KEYWORDS    RefSeq.
SOURCE      Sparus aurata (gilthead seabream)
  ORGANISM  Sparus aurata
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Spariformes; Sparidae; Sparus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044189.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900880675.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/21/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Sparus aurata"
                     /mol_type="mRNA"
                     /db_xref="taxon:8175"
                     /chromosome="3"
     gene            1..6795
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 5
                     ESTs, 4 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 14 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:115578518"
     CDS             177..2813
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_030267378.1"
                     /db_xref="GeneID:115578518"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQVTSTEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    258..647
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    678..830
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    831..1193
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(987..989,1032..1034,1074..1076,1086..1088,
                     1140..1142,1161..1163,1167..1169)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1332..2684
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1776..1778,1788..1790,1824..1835,1842..1844,
                     1866..1868,1875..1877,1887..1889,1899..1901)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1980..1982,1986..1988,2238..2240,2652..2654)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

agaaccagcagcaccagcggtagctcaggtcagtcagtccagtcagcagtcatccacgcaactatatattcccttttcttaaattagccactttaagcttcgcgataccgcccccaatttcagcgtcggtacggagccgtcaggccgggacagagacggagagagaggcctcggccatggaagcgctcggacccggccctcctgccccgacctctctgtttcagcctccgcggcgtcccggcctcggcacggtggggaaacccatccggctccttgccaaccactttcaggtccagattcccaagattgatgtctatcactatgatatcgacatcaagcctgagaaacggcctcggagggtcaacagggaagtggtggacaccatggtgcggcacttcaagatgcagatctttggagaccgacagcctggctacgatgggaagaggaacatgtacacggcacatccactgccgattggaagagacagggtggatctggaggtgaccctgccaggcgaggggaaagatcagacgttcaaggtgtctttgcagtgggtgtctgtggtcagtcttcagatgctgctcgaagctctttcaggtcaccttaacgaggtccctgaagactccgttcaggctctggatgtcatcacacggcatctgccttccatgaggtatactccagtggggcgttcattcttctcccctccggagggctattaccatcctctgggtggaggcagggaggtgtggtttggtttccatcagtctgtccgtcctgccatgtggaacatgatgctcaacatcgatgtgtcagcaactgctttctaccgtgctcagcctgtgatagagttcatgtgcgaggtgctggatatacagaacatcaacgaacagaccaaacccctcaccgactcccagcgtgtcaagttcaccaaggaaataagaggcttgaaagttgaggtcacacactgtggtcaaatgaagaggaagtaccgtgtgtgcaatgtaacgcgccgacctgccagccaccaaacgtttcccttacagcttgagaacggccaagccatggagtgcacagttgctcagtatttcaagcagaagtacaatctacagctcaagtatccacatttaccctgcctgcaagtagggcaagaacagaagcacacctaccttcccctagaggtctgtaacatagtagccggccaacgctgtatcaagaaactgacagacaaccagacatccaccatgatcaaagctacagctcgctcagcccccgacagacaggaagagatcagcagactggtcaaaagtaacagcatggtcgggggcccagacccttacctgaaggagtttggcattgtggtgcacaatgacatgacggaggtgacgggccgtgttctcccagcacccatgctacaatatgggggccgggtgagtacagacaccggaagggactgtggcaggaacaaaaccgtggccacgcccaatcagggcgtgtgggacatgagagggaagcagttctatgccggcatcgagatcaaggtctgggctgtggcctgcttcgccccacagaaacagtgtcgagaggacctgctcaagagtttcactgaccagcttcgaaagatctccaaagacgccgggatgcccattcaaggccagccatgtttctgtaaatacgcccaaggagctgacagcgtggagcccatgttcaaacacctcaagatgtcttatgtaggtctgcagctgattgtggtcatcctgcctggcaaaacaccagtctatgctgaggtgaagcgggtgggggatactctccttggcatggcaacccagtgtgtgcaggtgaagaacgtagtgaagacgtcccctcagaccctctccaacctgtgcctcaagatcaacgccaagctgggcggcatcaacaacgttctggtgcctcatcagaggccgtctgtgttccagcagcctgtcatcttcctgggtgcagatgtcacacatcctccagcgggtgacgggaagaagccgtccatcgctgcagtagtgggcagcatggacggccaccccagccgttactgtgcgacagtgcgtgtccaaacatccaggcaggacatgtcgcaggtaacatcgactgagcaactcttcagccaggaggtaatccaagacctgaccaacatggtgcgagagctgctcatccagttctacaagtccacgcgcttcaaacccacaagaatcatctattaccgtgggggtgtgtcagagggacaaatgaaacaggtcgcgtggccggagctgatagccatcaggaaggcatgcatcagtctggaggaggattacaggccgggcatcacctacatagtggttcagaaacgccaccacactcgtctgttctgctctgacaaagctgagagggtcggtaagagcggtaatgtcccagcaggcactacagtggacagcaccatcacacacccgtccgagtttgacttctacctgtgcagccatgctggaattcagggaaccagtcgtccctcccactaccacgtcctgtgggatgacaactgtttcactgccgatgaactgcagctcctcacctaccagctgtgccacacctacgtccgctgcacgcgctccgtctccatcccagcaccggcctactacgccaggctagtggctttccgagcccgctatcacttggtggacaaagaccacgacagtgctgaaggcagccacgtgtcgggtcaaagtaacggacgggacccccaggcgttggccaaggcagttcagatccactatgatacccagcacaccatgtacttcgcctgagctcgcgagcacgtcagccagtccacccgcggattcccttttctctctgtctttttatctcttatttgccagtctctctctctctctctctctctctctcctcttccttcacatctctttccttgtagtctgtctctttctcttcacactcactctactcatttttctgaatctgcaccaaagcggctcttggacgggaaaaatctgctgcgactcaaagtccagcaggtttttgtttttaaaggaacaatctccaaccagcgttcccaattgagccgcctttcaaaccagcaatcaagcactgcgaaccctgagggtttccagtgtgcaaaagaaaaaacaaaccatacacgcatactacaagttgagccattattttaagtttttttttctatttgaatgtctgcactcttgtgttttgtaagatacactgagcaagggagtggattggcacagtctgtgtcagcgcagctctttagctctcccagctaagcaggactcttatctacttttacctcaaagcccctttgggcaaaatctgtcacaaggtcttgggtttattgggtggtggggagggaactctacaggaaaagagacgaaggggagtcgaccttttgagaatgatatatatatatatctatacatatatatattttcctttttatgaacgtgtgttttaacttttctcctttaccgtcttttacaagcgaggtgacctgggtgtcatgcccataaactactttcctcccatcatagcgaagaatacgcaagtctttgtgagtgaatgtgtgtgtgcgcgcgtgtgtgtgtgcgtgtgtgtgtgtgcgtgcgcatctctgtatatacatgtactgctgtatgagagtgtgtttgtgtgtgtgcgcatgtgtttcatggcaggccactgaattgtaaagggaaatcaaggagataactgctgtaaatgcctcagatttgttgtttttatattcacacatacagtacatataagcatatatttacatctatagagcaactctgagacatatgacttaaacaggcctcgtggaggctggtgtgggtatttgtagcagtcctggtttgcttatagattgaaatgttttaaattgcatttcactgcgagcgtgagtttagtgggatttgcacctttgtgcctcagaactccgttttaatcccagaacaaaggtgatttaaatcatttcaagtgtaagtgaagtcaggtctgatgtgtagcagtagtaaccgtggtctggcctgtgactggctcagcattgggacctgttaaaaaggcagaaggcattaaacacagagcatggtcttatcaaccttgctttctgtgtgagaagcaggatcttatttttctttggacctgattatctgaaagattatatgatgacacggctggacgttgcttttctttttttttctttttttttttgttttctttttttagtagtttcaagggccggaaaccaacgtagttttttgaggtctcccttgttaatactggccaattcattgcactgacccgggacgctaggcaagcaacagagggcactctcaatctctcgtacctttatgcatttatacatacgaatcaacaaagcagatttgtaaaagtttaatataagatgttttgtggttatcttaaaaaaaaaaaactgttgtttagaattagtagtttaccattgatgttgttgttgttatcgttgttgtcaatgttgttactattgtgatgaaattttacggttggcagcaaagccagttatagttactagtgcgacttcaggaatagacttcagttgttgagctgcaggtttaacggggttagagctttgggttggaactttttttttttttttttttgtagcattctggttaatggtgagaaggagcagtaggccccaggatggatgaattcatgcttcctctgtaggtttttgaggtaatgtgtagcactccgactggtaggacagatctgccgctgtgtacatcactcaggtggtttggatcagatagatggagacaatcagggattgtaatcagagtgaaactagattaagggattggttaaaaaacggtctgcgaaaggcaatgagttgaatttttagactttttaaaaacggtttctattcctctgtgtgtatttgaagtatacccatcttaaagtctctgctatttacggtgtttgtttcatatttttatgcatttttaacactggattaaatgaattgacaataagcataagcactgcccttggaccccctgtacaaacactcagcacactgtccaccatgaaatccggtggcagttgcacaaatggtgccccctggtgttggagggagagtttttggaggtagaggtcgacgtgcacgcctcatcagggggggacagctcagtggaagcccttttgtaataaaccacccgtaaagcaacaggtttactatgaaatgagtttgagcacctgcctgttggaggcgtgggcgttggcatcggggaacagagaagaaggaaatgagggagcagcggggggggattgggagggtgtcgtcgtggcattgcatgcaataagttttactctttgcagatcaatttttaaaaaaacgtgcactagttttaccaagccaagcgggctgtgattgaatattcatctggcatctctaacggggaatgatttgttttatatccttcacaatattaaactccagtcagatcagtgatcactccctgtgttttgtaacattgcgaagcaatattaacccagttctagttttcaaatgtggaaaaacaaagctataattctactcattgtggttgttggtctactctcgcaaattgtgtctctgtacttttttatttttttgttatttttagtttattttatttgcttgtatcaatcagtgggaatgtgcatttggtggaatagttatgtaggctttagacaatttttctttttaaagtaacaaaatgtagactttgctcgcctgcatgccagagtattgtcctttttaaaaagaaaaaaaaaaagaagaaaaaaacctttttggaatcatatttattatgagaaagaaaaaaatattaaaagcttttttttggtaaatgtttatcaatttagtgggactgttttaggctagcaatatttgtccagtctctctaagatacattttaacgattcctacacaaatgtcagtaatttgggaggtacactctgttttctgaggcaaggggggatcggctctttgcgtcgtgttcaaattcaaacgaggtactttcttcaatcacggacccgtcaaagacccggcccctctcgtcttccccaggttactgtagcgagcgtccattttgtctcctcgttcctggtttgtacctgtggccacgcctctcggtgggggctctcctgtcaccatggcatcagtggagcaggattacctctttttaacctttttaaaaaaaacgatccataattctttctgtgccccgatagaaagagagcatgaattatttttaaattcctctcactttgatatattgttatatcttaacggatttgtaatttgacattgtgtattgtgtatttttattttattttgttttttcctttttttggttccttattttcggggggttgttatagctctgctctgtttcttttgttgtttttttctgctagtggtctctgaagctgtgtgtgattgtctaatagcaataagaatcacagagttggaacattccaatttctgtgaatggaacgttccgattctgtgaatgaacgtagccctttctgagcggttgtgagggcggggcatgttctagccccgccccccagcagagggccattcactcgctgttattgctattatgtgacatgttcaaataccttttttttcttttcttttgttttttttttttttttttttagcctgcagggaattcttgtgttaatgtgcaaagtaataaattggtattttatgcctataacaca

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